Acid sphingomyelinase-deficient human lymphoblasts and mice are defective in radiation-induced apoptosis - PubMed (original) (raw)
Comparative Study
. 1996 Jul 26;86(2):189-99.
doi: 10.1016/s0092-8674(00)80091-4.
Affiliations
- PMID: 8706124
- DOI: 10.1016/s0092-8674(00)80091-4
Free article
Comparative Study
Acid sphingomyelinase-deficient human lymphoblasts and mice are defective in radiation-induced apoptosis
P Santana et al. Cell. 1996.
Free article
Abstract
Stress is believed to activate sphingomyelinase to generate ceramide, which serves as a second messenger in initiating the apoptotic response. Conclusive evidence for this paradigm, however, is lacking. In the present study, we used a genetic approach to address this issue directly. We show that lymphoblasts from Niemann-Pick patients, which have an inherited deficiency of acid sphingomyelinase activity, fail to respond to ionizing radiation with ceramide generation and apoptosis. These abnormalities are reversible up on restoration of acid sphingomyelinase activity by retroviral transfer of human acid sphingomyelinase cDNA. Acid sphingomyelinase knockout mice also expressed defects in radiation-induced ceramide generation and apoptosis in vivo. Comparison with p53 knockout mice revealed that acid sphingomyelinase-mediated apoptosis and p53-mediated apoptosis are likely distinct and independent. These genetic models provide definitive evidence for the involvement of acid sphingomyelinase in one form of stress-induced apoptosis.
Similar articles
- Cell autonomous apoptosis defects in acid sphingomyelinase knockout fibroblasts.
Lozano J, Menendez S, Morales A, Ehleiter D, Liao WC, Wagman R, Haimovitz-Friedman A, Fuks Z, Kolesnick R. Lozano J, et al. J Biol Chem. 2001 Jan 5;276(1):442-8. doi: 10.1074/jbc.M006353200. J Biol Chem. 2001. PMID: 11031259 - Signaling pathway for radiation-induced apoptosis in the lymphoblasts from neuronopathic (type A) and non-neuronopathic (type B) forms of Niemann-Pick disease.
Mikami T, Takahashi T, Ishida A, Minamiya Y, Ida H, Takada G. Mikami T, et al. J Neurol Sci. 2002 Jul 15;199(1-2):39-43. doi: 10.1016/s0022-510x(02)00106-5. J Neurol Sci. 2002. PMID: 12084440 - Lysosomal sphingomyelinase is not solicited for apoptosis signaling.
Bezombes C, Ségui B, Cuvillier O, Bruno AP, Uro-Coste E, Gouazé V, Andrieu-Abadie N, Carpentier S, Laurent G, Salvayre R, Jaffrézou JP, Levade T. Bezombes C, et al. FASEB J. 2001 Feb;15(2):297-9. doi: 10.1096/fj.00-0466fje. Epub 2000 Dec 8. FASEB J. 2001. PMID: 11156942 - Acid sphingomyelinase, cell membranes and human disease: lessons from Niemann-Pick disease.
Schuchman EH. Schuchman EH. FEBS Lett. 2010 May 3;584(9):1895-900. doi: 10.1016/j.febslet.2009.11.083. Epub 2009 Nov 26. FEBS Lett. 2010. PMID: 19944693 Review. - [Niemann-Pick disease types A and B].
Ohno K. Ohno K. Nihon Rinsho. 1995 Dec;53(12):3014-8. Nihon Rinsho. 1995. PMID: 8577051 Review. Japanese.
Cited by
- Radiation-induced gastrointestinal and cutaneous injuries: understanding models, pathologies, assessments, and clinically accepted practices.
Tamarat R, Satyamitra MM, Benderitter M, DiCarlo AL. Tamarat R, et al. Int J Radiat Biol. 2024;100(7):969-981. doi: 10.1080/09553002.2024.2356544. Epub 2024 May 24. Int J Radiat Biol. 2024. PMID: 38787685 Free PMC article. - Diabetic retinopathy is a ceramidopathy reversible by anti-ceramide immunotherapy.
Dorweiler TF, Singh A, Ganju A, Lydic TA, Glazer LC, Kolesnick RN, Busik JV. Dorweiler TF, et al. Cell Metab. 2024 Jul 2;36(7):1521-1533.e5. doi: 10.1016/j.cmet.2024.04.013. Epub 2024 May 7. Cell Metab. 2024. PMID: 38718792 - Blocking lipid synthesis induces DNA damage in prostate cancer and increases cell death caused by PARP inhibition.
Ribeiro CF, Rodrigues S, Bastos DC, Fanelli GN, Pakula H, Foiani M, Zadra G, Loda M. Ribeiro CF, et al. Sci Signal. 2024 Apr 9;17(831):eadh1922. doi: 10.1126/scisignal.adh1922. Epub 2024 Apr 9. Sci Signal. 2024. PMID: 38593154 Free PMC article. - Cell Death, by Any Other Name….
Kandouz M. Kandouz M. Cells. 2024 Feb 10;13(4):325. doi: 10.3390/cells13040325. Cells. 2024. PMID: 38391938 Free PMC article. Review. - Ceramides are fuel gauges on the drive to cardiometabolic disease.
Wilkerson JL, Tatum SM, Holland WL, Summers SA. Wilkerson JL, et al. Physiol Rev. 2024 Jul 1;104(3):1061-1119. doi: 10.1152/physrev.00008.2023. Epub 2024 Feb 1. Physiol Rev. 2024. PMID: 38300524 Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Research Materials
Miscellaneous