Expression of a constitutively active Akt Ser/Thr kinase in 3T3-L1 adipocytes stimulates glucose uptake and glucose transporter 4 translocation - PubMed (original) (raw)
. 1996 Dec 6;271(49):31372-8.
doi: 10.1074/jbc.271.49.31372.
Affiliations
- PMID: 8940145
- DOI: 10.1074/jbc.271.49.31372
Free article
Expression of a constitutively active Akt Ser/Thr kinase in 3T3-L1 adipocytes stimulates glucose uptake and glucose transporter 4 translocation
A D Kohn et al. J Biol Chem. 1996.
Free article
Abstract
Akt is a serine/threonine kinase that requires a functional phosphatidylinositol 3-kinase to be stimulated by insulin and other growth factors. When directed to membranes by the addition of a src myristoylation sequence, Akt becomes constitutively active. In the present studies, the constitutively active Akt and a nonmyristoylated control mutant were expressed in 3T3-L1 cells that can be induced to differentiate into adipocytes. The constitutively active Akt induced glucose uptake into adipocytes in the absence of insulin by stimulating translocation of the insulin-responsive glucose transporter 4 to the plasma membrane. The constitutively active Akt also increased the synthesis of the ubiquitously expressed glucose transporter 1. The increased glucose influx in the 3T3-L1 adipocytes directed lipid but not glycogen synthesis. These results indicate that Akt can regulate glucose uptake and metabolism.
Similar articles
- Activation of the mammalian target of rapamycin pathway acutely inhibits insulin signaling to Akt and glucose transport in 3T3-L1 and human adipocytes.
Tremblay F, Gagnon A, Veilleux A, Sorisky A, Marette A. Tremblay F, et al. Endocrinology. 2005 Mar;146(3):1328-37. doi: 10.1210/en.2004-0777. Epub 2004 Dec 2. Endocrinology. 2005. PMID: 15576463 - Membrane localization of 3-phosphoinositide-dependent protein kinase-1 stimulates activities of Akt and atypical protein kinase C but does not stimulate glucose transport and glycogen synthesis in 3T3-L1 adipocytes.
Egawa K, Maegawa H, Shi K, Nakamura T, Obata T, Yoshizaki T, Morino K, Shimizu S, Nishio Y, Suzuki E, Kashiwagi A. Egawa K, et al. J Biol Chem. 2002 Oct 11;277(41):38863-9. doi: 10.1074/jbc.M203132200. Epub 2002 Jul 29. J Biol Chem. 2002. PMID: 12147684 - An inhibitor of p38 mitogen-activated protein kinase prevents insulin-stimulated glucose transport but not glucose transporter translocation in 3T3-L1 adipocytes and L6 myotubes.
Sweeney G, Somwar R, Ramlal T, Volchuk A, Ueyama A, Klip A. Sweeney G, et al. J Biol Chem. 1999 Apr 9;274(15):10071-8. doi: 10.1074/jbc.274.15.10071. J Biol Chem. 1999. PMID: 10187787 - A role for the serine/threonine kinase, Akt, in insulin-stimulated glucose uptake.
Summers SA, Birnbaum MJ. Summers SA, et al. Biochem Soc Trans. 1997 Aug;25(3):981-8. doi: 10.1042/bst0250981. Biochem Soc Trans. 1997. PMID: 9388586 Review. No abstract available. - Role of Akt/protein kinase B in metabolism.
Whiteman EL, Cho H, Birnbaum MJ. Whiteman EL, et al. Trends Endocrinol Metab. 2002 Dec;13(10):444-51. doi: 10.1016/s1043-2760(02)00662-8. Trends Endocrinol Metab. 2002. PMID: 12431841 Review.
Cited by
- Effect of metabolically divergent pig breeds and tissues on mesenchymal stem cell expression patterns during adipogenesis.
Ponsuksili S, Siengdee P, Li S, Kriangwanich W, Oster M, Reyer H, Wimmers K. Ponsuksili S, et al. BMC Genomics. 2024 Apr 25;25(1):407. doi: 10.1186/s12864-024-10308-z. BMC Genomics. 2024. PMID: 38664635 Free PMC article. - Presence of active AKT in the nucleus upon adipocyte differentiation of 3T3-L1 cells.
Goris M, Jacobsen RG, Lewis AE. Goris M, et al. MicroPubl Biol. 2024 Feb 29;2024:10.17912/micropub.biology.001140. doi: 10.17912/micropub.biology.001140. eCollection 2024. MicroPubl Biol. 2024. PMID: 38495585 Free PMC article. - Redox Regulation of PTEN by Reactive Oxygen Species: Its Role in Physiological Processes.
Trinh VH, Nguyen Huu T, Sah DK, Choi JM, Yoon HJ, Park SC, Jung YS, Lee SR. Trinh VH, et al. Antioxidants (Basel). 2024 Feb 4;13(2):199. doi: 10.3390/antiox13020199. Antioxidants (Basel). 2024. PMID: 38397797 Free PMC article. Review. - Ceramides are fuel gauges on the drive to cardiometabolic disease.
Wilkerson JL, Tatum SM, Holland WL, Summers SA. Wilkerson JL, et al. Physiol Rev. 2024 Jul 1;104(3):1061-1119. doi: 10.1152/physrev.00008.2023. Epub 2024 Feb 1. Physiol Rev. 2024. PMID: 38300524 Review. - Partners in diabetes epidemic: A global perspective.
Wang H, Akbari-Alavijeh S, Parhar RS, Gaugler R, Hashmi S. Wang H, et al. World J Diabetes. 2023 Oct 15;14(10):1463-1477. doi: 10.4239/wjd.v14.i10.1463. World J Diabetes. 2023. PMID: 37970124 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Miscellaneous