Frequent clones of p53-mutated keratinocytes in normal human skin - PubMed (original) (raw)
Frequent clones of p53-mutated keratinocytes in normal human skin
A S Jonason et al. Proc Natl Acad Sci U S A. 1996.
Abstract
The multiple genetic hit model of cancer predicts that normal individuals should have stable populations of cancer-prone, but noncancerous, mutant cells awaiting further genetic hits. We report that whole-mount preparations of human skin contain clonal patches of p53-mutated keratinocytes, arising from the dermal-epidermal junction and from hair follicles. These clones, 60-3000 cells in size, are present at frequencies exceeding 40 cells per cm2 and together involve as much as 4% of the epidermis. In sun-exposed skin, clones are both more frequent and larger than in sun-shielded skin. We conclude that, in addition to being a tumorigenic mutagen, sunlight acts as a tumor promoter by favoring the clonal expansion of p53-mutated cells. These combined actions of sunlight result in normal individuals carrying a substantial burden of keratinocytes predisposed to cancer.
Figures
Figure 1
p53-immunopositive clones in whole-mount preparations of human epidermis. Clones arising from (A) the dermal–epidermal junction and (B) a hair follicle. In both cases, view is from the basal surface to show follicles. (×100.)
Figure 2
Increase of clone frequency with sun exposure. Histogram shows the average frequency of p53-mutated patches from sun-shielded, intermittently exposed, and chronically sun-exposed skin. Mean patch frequency for intermittently exposed skin excludes the outlier. Bars represent SEM.
Figure 3
Conical clone of p53-mutated keratinocytes. Shown is the side view of a three-dimensionally reconstructed immunofluorescent cone from an epidermal whole mount. Opacity of the surrounding background epidermis has been adjusted to allow complete false-color visualization of all immunostaining cells in the_z_-scan. The cone’s apex lies at the bottom of the view, along the plane of the basal surface. Confocal images were captured using a Bio-Rad MRC 600 laser scanning confocal microscope with ×20 objective.
Figure 4
Increase of clone size with sun exposure. Dot histogram shows the area in mm2 of individual p53-mutated patches from sun-shielded, intermittently exposed, and chronically sun-exposed skin. Each point represents an individual clone.
Comment in
- Sunlight and skin cancer: another link revealed.
Kraemer KH. Kraemer KH. Proc Natl Acad Sci U S A. 1997 Jan 7;94(1):11-4. doi: 10.1073/pnas.94.1.11. Proc Natl Acad Sci U S A. 1997. PMID: 8990152 Free PMC article. Review. No abstract available.
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