Association of the parainfluenza virus fusion and hemagglutinin-neuraminidase glycoproteins on cell surfaces - PubMed (original) (raw)
Association of the parainfluenza virus fusion and hemagglutinin-neuraminidase glycoproteins on cell surfaces
Q Yao et al. J Virol. 1997 Jan.
Abstract
We previously observed that cell fusion caused by human parainfluenza virus type 2 or type 3 requires the expression of both the fusion (F) and hemagglutinin-neuraminidase (HN) glycoproteins from the same virus type, indicating that a type-specific interaction between F and HN is needed for the induction of cell fusion. In the present study we have further investigated the fusion properties of F and HN proteins of parainfluenza virus type 1 (PI1), type 2 (PI2), and type 3 (PI3), Sendai virus (SN), and simian virus 5 (SV5) by expression of their glycoprotein genes in HeLa T4 cells using the vaccinia virus-T7 transient expression system. Consistent with previous results, cell fusion was observed in cells transfected with homotypic F/HN proteins; with one exception, coexpression of any combination of F and HN proteins from different viruses did not result in cell fusion. The only exception was found with the closely related PI1 HN and SN HN glycoproteins, either of which could interact with SN F to induce cell fusion upon coexpression as previously reported. By specific labeling and coprecipitation of proteins expressed on the cell surface, we observed that anti-PI2 HN antiserum coprecipitated PI2 F when the homotypic PI2 F and PI2 HN were coexpressed, but not the F proteins of other paramyxoviruses when heterotypic F genes were coexpressed with PI2 HN, suggesting that the homotypic F and HN proteins are physically associated with each other on cell surfaces. Furthermore, we observed that PI3 F was found to cocap with PI3 HN but not with PI2 HN, also indicating a specific association between the homotypic proteins. These results indicate that the homotypic F and HN glycoproteins are physically associated with each other on the cell surface and suggest that such association is crucial to cell fusion induced by paramyxoviruses.
Similar articles
- Functional interactions between the fusion protein and hemagglutinin-neuraminidase of human parainfluenza viruses.
Hu XL, Ray R, Compans RW. Hu XL, et al. J Virol. 1992 Mar;66(3):1528-34. doi: 10.1128/JVI.66.3.1528-1534.1992. J Virol. 1992. PMID: 1310764 Free PMC article. - Alternative mechanisms of interaction between homotypic and heterotypic parainfluenza virus HN and F proteins.
Tong S, Compans RW. Tong S, et al. J Gen Virol. 1999 Jan;80 ( Pt 1):107-115. doi: 10.1099/0022-1317-80-1-107. J Gen Virol. 1999. PMID: 9934692 - Paramyxovirus mediated cell fusion requires co-expression of both the fusion and hemagglutinin-neuraminidase glycoproteins.
Heminway BR, Yu Y, Galinski MS. Heminway BR, et al. Virus Res. 1994 Jan;31(1):1-16. doi: 10.1016/0168-1702(94)90066-3. Virus Res. 1994. PMID: 8165862 - Paramyxovirus membrane fusion: lessons from the F and HN atomic structures.
Lamb RA, Paterson RG, Jardetzky TS. Lamb RA, et al. Virology. 2006 Jan 5;344(1):30-7. doi: 10.1016/j.virol.2005.09.007. Virology. 2006. PMID: 16364733 Free PMC article. Review.
Cited by
- The Respiratory Syncytial Virus (RSV) G Protein Enhances the Immune Responses to the RSV F Protein in an Enveloped Virus-Like Particle Vaccine Candidate.
McGinnes Cullen L, Luo B, Wen Z, Zhang L, Durr E, Morrison TG. McGinnes Cullen L, et al. J Virol. 2023 Jan 31;97(1):e0190022. doi: 10.1128/jvi.01900-22. Epub 2023 Jan 5. J Virol. 2023. PMID: 36602367 Free PMC article. - Vimentin inhibits α-tubulin acetylation via enhancing α-TAT1 degradation to suppress the replication of human parainfluenza virus type 3.
Liu P, Zhang S, Ma J, Jin D, Qin Y, Chen M. Liu P, et al. PLoS Pathog. 2022 Sep 15;18(9):e1010856. doi: 10.1371/journal.ppat.1010856. eCollection 2022 Sep. PLoS Pathog. 2022. PMID: 36108090 Free PMC article. - Differential In Vitro Growth and Cell Killing of Cancer versus Benign Prostate Cells by Oncolytic Parainfluenza Virus.
Kedarinath K, Parks GD. Kedarinath K, et al. Pathogens. 2022 Apr 21;11(5):493. doi: 10.3390/pathogens11050493. Pathogens. 2022. PMID: 35631014 Free PMC article. - Antivirals targeting paramyxovirus membrane fusion.
Contreras EM, Monreal IA, Ruvalcaba M, Ortega V, Aguilar HC. Contreras EM, et al. Curr Opin Virol. 2021 Dec;51:34-47. doi: 10.1016/j.coviro.2021.09.003. Epub 2021 Sep 27. Curr Opin Virol. 2021. PMID: 34592709 Free PMC article. Review. - Genetic Characteristics of Human Parainfluenza Virus Types 1-4 From Patients With Clinical Respiratory Tract Infection in China.
Shao N, Liu B, Xiao Y, Wang X, Ren L, Dong J, Sun L, Zhu Y, Zhang T, Yang F. Shao N, et al. Front Microbiol. 2021 Jul 15;12:679246. doi: 10.3389/fmicb.2021.679246. eCollection 2021. Front Microbiol. 2021. PMID: 34335501 Free PMC article.
References
- Nature. 1994 Sep 1;371(6492):37-43 - PubMed
- Rev Infect Dis. 1980 Jan-Feb;2(1):40-61 - PubMed
- J Virol. 1994 Nov;68(11):7654-8 - PubMed
- Virology. 1994 Nov 1;204(2):506-14 - PubMed
- Virology. 1995 Jun 1;209(2):457-69 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Research Materials
Miscellaneous