Structural determinants of the interaction between the erbB2 receptor and the Src homology 2 domain of Grb7 - PubMed (original) (raw)
. 1997 Mar 28;272(13):8490-7.
doi: 10.1074/jbc.272.13.8490.
Affiliations
- PMID: 9079677
- DOI: 10.1074/jbc.272.13.8490
Free article
Structural determinants of the interaction between the erbB2 receptor and the Src homology 2 domain of Grb7
P W Janes et al. J Biol Chem. 1997.
Free article
Abstract
The Src homology 2 (SH2) domain-containing protein Grb7 and the erbB2 receptor tyrosine kinase are overexpressed in a subset of human breast cancers. They also co-immunoprecipitate from cell lysates and associate directly in vitro. Whereas the Grb7 SH2 domain binds strongly to erbB2, the SH2 domain of Grb14, a protein closely related to Grb7, does not. We have investigated the preferred binding site of Grb7 within the erbB2 intracellular domain and the SH2 domain residues that determine the high affinity of Grb7 compared with Grb14 for this site. Phosphopeptide competition and site-directed mutagenesis revealed that Tyr-1139 of erbB2 is the major binding site for the Grb7 SH2 domain, indicating an overlap in binding specificity between the Grb7 and Grb2 SH2 domains. Substituting individual amino acids in the Grb14 SH2 domain with the corresponding residues from Grb7 demonstrated that a Gln to Leu change at the betaD6 position imparted high affinity erbB2 interaction, paralleled by a marked increase in affinity for the Tyr-1139 phosphopeptide. The reverse switch at the betaD6 position abrogated Grb7 binding to erbB2. This residue therefore represents an important determinant of SH2 domain specificity within the Grb7 family.
Similar articles
- Solution structure of the human Grb14-SH2 domain and comparison with the structures of the human Grb7-SH2/erbB2 peptide complex and human Grb10-SH2 domain.
Scharf PJ, Witney J, Daly R, Lyons BA. Scharf PJ, et al. Protein Sci. 2004 Sep;13(9):2541-6. doi: 10.1110/ps.04884704. Protein Sci. 2004. PMID: 15322292 Free PMC article. - Identification of novel non-phosphorylated ligands, which bind selectively to the SH2 domain of Grb7.
Pero SC, Oligino L, Daly RJ, Soden AL, Liu C, Roller PP, Li P, Krag DN. Pero SC, et al. J Biol Chem. 2002 Apr 5;277(14):11918-26. doi: 10.1074/jbc.M111816200. Epub 2002 Jan 24. J Biol Chem. 2002. PMID: 11809769 - Structural basis for dimerization of the Grb10 Src homology 2 domain. Implications for ligand specificity.
Stein EG, Ghirlando R, Hubbard SR. Stein EG, et al. J Biol Chem. 2003 Apr 11;278(15):13257-64. doi: 10.1074/jbc.M212026200. Epub 2003 Jan 27. J Biol Chem. 2003. PMID: 12551896 - The intertwining of structure and function: proposed helix-swapping of the SH2 domain of Grb7, a regulatory protein implicated in cancer progression and inflammation.
Pias S, Peterson TA, Johnson DL, Lyons BA. Pias S, et al. Crit Rev Immunol. 2010;30(3):299-304. doi: 10.1615/critrevimmunol.v30.i3.70. Crit Rev Immunol. 2010. PMID: 20370637 Free PMC article. Review. - The Grb7 family of signalling proteins.
Daly RJ. Daly RJ. Cell Signal. 1998 Oct;10(9):613-8. doi: 10.1016/s0898-6568(98)00022-9. Cell Signal. 1998. PMID: 9794242 Review.
Cited by
- GRB7-mediated enhancement of cell malignant characteristics induced by Helicobacter pylori infection.
Zhao H, Chen S, Bai X, Zhang J, Liu S, Sun Z, Cao X, Wang J, Zhang Y, Li B, Ji X. Zhao H, et al. Front Microbiol. 2024 Sep 18;15:1469953. doi: 10.3389/fmicb.2024.1469953. eCollection 2024. Front Microbiol. 2024. PMID: 39360313 Free PMC article. - TCF12-regulated GRB7 facilitates the HER2+ breast cancer progression by activating Notch1 signaling pathway.
Wang G, Wu Y, Su Y, Qu N, Chen B, Zhou D, Yuan L, Yin M, Liu M, Zhou W. Wang G, et al. J Transl Med. 2024 Aug 7;22(1):745. doi: 10.1186/s12967-024-05536-6. J Transl Med. 2024. PMID: 39113057 Free PMC article. - Enhancing the Bioactivity of Bicyclic Peptides Targeted to Grb7-SH2 by Restoring Cell Permeability.
Sturre NP, Colson RN, Shah N, Watson GM, Yang X, Wilce MCJ, Price JT, Wilce JA. Sturre NP, et al. Biomedicines. 2022 May 16;10(5):1145. doi: 10.3390/biomedicines10051145. Biomedicines. 2022. PMID: 35625882 Free PMC article. - The intracellular domains of the EphB6 and EphA10 receptor tyrosine pseudokinases function as dynamic signalling hubs.
Liang LY, Roy M, Horne CR, Sandow JJ, Surudoi M, Dagley LF, Young SN, Dite T, Babon JJ, Janes PW, Patel O, Murphy JM, Lucet IS. Liang LY, et al. Biochem J. 2021 Sep 17;478(17):3351-3371. doi: 10.1042/BCJ20210572. Biochem J. 2021. PMID: 34431498 Free PMC article. - Store-Operated Calcium Entry: Shaping the Transcriptional and Epigenetic Landscape in Pancreatic Cancer.
Kutschat AP, Johnsen SA, Hamdan FH. Kutschat AP, et al. Cells. 2021 Apr 21;10(5):966. doi: 10.3390/cells10050966. Cells. 2021. PMID: 33919156 Free PMC article. Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Research Materials
Miscellaneous