CD40 mediated activation of gingival and periodontal ligament fibroblasts - PubMed (original) (raw)
CD40 mediated activation of gingival and periodontal ligament fibroblasts
G D Sempowski et al. J Periodontol. 1997 Mar.
Abstract
CD40 is a 50 kDa transmembrane protein important for regulating B lymphocyte proliferation and differentiation. This novel activation antigen is primarily expressed by hematopoietic cells including B lymphocytes, follicular dendritic cells, and monocytes. Recently, human fibroblasts from a variety of tissues were shown to display CD40; however, its function was unknown. Cellular responses mediated by CD40 are naturally triggered by its counter-receptor, the CD40 ligand, which is displayed on activated T cells, mast cells, eosinophils, basophils, and B lineage cells. This study investigated the functional significance of CD40 expression on periodontal fibroblasts, in the context of periodontal inflammation. The experiments reported herein demonstrate constitutive CD40 expression on cultured periodontal ligament (PDL) and gingival fibroblasts. Interestingly, cells of gingival origin displayed up to 13-fold higher constitutive levels of CD40, versus fibroblasts from PDL. Interferon gamma (IFN gamma) treatment enhanced CD40 expression on PDL and gingival fibroblasts, with up to 61-fold induction of expression. Immunohistochemical staining was used to detect CD40 on fibroblastic cells in both normal and acutely inflamed gingival tissue. Expression of CD40 in inflamed tissue was significantly higher than in uninflamed tissue. Western blot analysis of anti-CD40 triggered cells revealed the induction of tyrosine phosphorylation on a 50 kDa protein in PDL and gingival fibroblasts. These results indicate that CD40 is an active signaling conduit in periodontal fibroblasts. This concept was further substantiated by the fact that CD40 engagement stimulated interleukin 6 (IL-6) production by gingival fibroblasts, but not periodontal ligament fibroblasts. Overall, these results demonstrate that CD40 on periodontal fibroblasts may functionally interact with CD40L-expressing cells. This CD40/CD40L interaction can stimulate fibroblast activation and synthesis of the proinflammatory cytokine IL-6.
Similar articles
- Role of cytokine in the induction of adhesion molecules on cultured human gingival fibroblasts.
Takahashi K, Takigawa M, Takashiba S, Nagai A, Miyamoto M, Kurihara H, Murayama Y. Takahashi K, et al. J Periodontol. 1994 Mar;65(3):230-5. doi: 10.1902/jop.1994.65.3.230. J Periodontol. 1994. PMID: 7513022 - Periodontal ligament and gingival fibroblasts from periodontitis patients are more active in interaction with Porphyromonas gingivalis.
Scheres N, Laine ML, Sipos PM, Bosch-Tijhof CJ, Crielaard W, de Vries TJ, Everts V. Scheres N, et al. J Periodontal Res. 2011 Aug;46(4):407-16. doi: 10.1111/j.1600-0765.2011.01353.x. Epub 2011 Feb 17. J Periodontal Res. 2011. PMID: 21332474 - Accessory signaling by CD40 for T cell activation: induction of Th1 and Th2 cytokines and synergy with interleukin-12 for interferon-gamma production.
Peng X, Kasran A, Warmerdam PA, de Boer M, Ceuppens JL. Peng X, et al. Eur J Immunol. 1996 Jul;26(7):1621-7. doi: 10.1002/eji.1830260732. Eur J Immunol. 1996. PMID: 8766570 - The CD40 antigen and its ligand.
Banchereau J, Bazan F, Blanchard D, Brière F, Galizzi JP, van Kooten C, Liu YJ, Rousset F, Saeland S. Banchereau J, et al. Annu Rev Immunol. 1994;12:881-922. doi: 10.1146/annurev.iy.12.040194.004313. Annu Rev Immunol. 1994. PMID: 7516669 Review. - Perspective of cytokine regulation for periodontal treatment: fibroblast biology.
Takashiba S, Naruishi K, Murayama Y. Takashiba S, et al. J Periodontol. 2003 Jan;74(1):103-10. doi: 10.1902/jop.2003.74.1.103. J Periodontol. 2003. PMID: 12593604 Review.
Cited by
- Immunoexpression of interferon-gamma in the interdental gingiva of chronic periodontitis patients with interferon-gamma (+874A/T) rs62559044 polymorphism.
Sheibak N, Heidari Z, Mahmoudzadeh-Sagheb H. Sheibak N, et al. J Oral Biol Craniofac Res. 2022 Sep-Oct;12(5):727-732. doi: 10.1016/j.jobcr.2022.08.019. Epub 2022 Aug 26. J Oral Biol Craniofac Res. 2022. PMID: 36106180 Free PMC article. - CD18 Mediates Neutrophil Imperviousness to the Aggregatibacter actinomycetemcomitans JP2 Clone in Molar-Incisor Pattern Periodontitis.
Hashai K, Chapple IL, Shapira L, Assadi W, Dadon S, Polak D. Hashai K, et al. Front Immunol. 2022 May 18;13:847372. doi: 10.3389/fimmu.2022.847372. eCollection 2022. Front Immunol. 2022. PMID: 35663998 Free PMC article. - Dermal Drivers of Injury-Induced Inflammation: Contribution of Adipocytes and Fibroblasts.
Cooper PO, Haas MR, Noonepalle SKR, Shook BA. Cooper PO, et al. Int J Mol Sci. 2021 Feb 16;22(4):1933. doi: 10.3390/ijms22041933. Int J Mol Sci. 2021. PMID: 33669239 Free PMC article. Review. - Defensin production by human limbo-corneal fibroblasts infected with mycobacteria.
Castañeda-Sánchez JI, García-Pérez BE, Muñoz-Duarte AR, Baltierra-Uribe SL, Mejia-López H, López-López C, Bautista-De Lucio VM, Robles-Contreras A, Luna-Herrera J. Castañeda-Sánchez JI, et al. Pathogens. 2013 Feb 4;2(1):13-32. doi: 10.3390/pathogens2010013. Pathogens. 2013. PMID: 25436879 Free PMC article. - Soluble CD40 ligand: a novel biomarker in the pathogenesis of periodontal disease.
Chaturvedi R, Gupta M, Jain A, Das T, Prashar S. Chaturvedi R, et al. Clin Oral Investig. 2015 Jan;19(1):45-52. doi: 10.1007/s00784-014-1216-3. Epub 2014 Mar 5. Clin Oral Investig. 2015. PMID: 24595688
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials