Beta2-agonists prevent Th1 development by selective inhibition of interleukin 12 - PubMed (original) (raw)

Beta2-agonists prevent Th1 development by selective inhibition of interleukin 12

P Panina-Bordignon et al. J Clin Invest. 1997.

Abstract

Interleukin 12 (IL-12) plays a central role in the immune system by skewing the immune response towards T helper 1 (Th1) type responses which are characterized by high interferon-gamma and low IL-4 production. In this report we present evidence that beta2-agonists inhibit IL-12 production by both human monocytes in response to lipopolysaccharide (LPS) and dendritic cells stimulated via CD40. Inhibition of IL-12 production is selective, as other cytokines produced by monocytes are unaffected. IL-12 inhibition is dependent on beta2-adrenoceptor stimulation and correlates with increased levels of intracellular cAMP. In conjunction with their ability to suppress IL-12 production, when beta2-agonists are added at priming of neonatal T lymphocytes, they inhibit the development of Th1-type cells, while promoting T helper 2 (Th2) cell differentiation. Further, the in vivo administration of a therapeutic dose of salbutamol results in the selective inhibition of IL-12 production by whole blood lymphocytes stimulated in vitro with LPS. These findings provide new insight into the immunological consequences of the clinical use of beta2-agonists and may suggest new approaches for the treatment of Th1-mediated diseases.

PubMed Disclaimer

Similar articles

• Curcumin inhibits Th1 cytokine profile in CD4+ T cells by suppressing interleukin-12 production in macrophages.
  Kang BY, Song YJ, Kim KM, Choe YK, Hwang SY, Kim TS. Kang BY, et al. Br J Pharmacol. 1999 Sep;128(2):380-4. doi: 10.1038/sj.bjp.0702803. Br J Pharmacol. 1999. PMID: 10510448 Free PMC article.
• Impact of plastic adhesion in vitro on analysis of Th1 and Th2 cytokines and immune cell distribution from mice with multiple low-dose streptozotocin-induced diabetes.
  Thorvaldson L, Johansson SE, Höglund P, Sandler S. Thorvaldson L, et al. J Immunol Methods. 2005 Dec 20;307(1-2):73-81. doi: 10.1016/j.jim.2005.09.008. Epub 2005 Oct 17. J Immunol Methods. 2005. PMID: 16263129
• Cytokine profiles of canine monocyte-derived dendritic cells as a function of lipopolysaccharide- or tumor necrosis factor-alpha-induced maturation.
  Wang YS, Chi KH, Chu RM. Wang YS, et al. Vet Immunol Immunopathol. 2007 Aug 15;118(3-4):186-98. doi: 10.1016/j.vetimm.2007.05.010. Epub 2007 Jun 2. Vet Immunol Immunopathol. 2007. PMID: 17617471
• IL-12 as a therapeutic target for pharmacological modulation in immune-mediated and inflammatory diseases: regulation of T helper 1/T helper 2 responses.
  Haskó G, Szabó C. Haskó G, et al. Br J Pharmacol. 1999 Jul;127(6):1295-304. doi: 10.1038/sj.bjp.0702689. Br J Pharmacol. 1999. PMID: 10455278 Free PMC article. Review.
• TH1/TH2 cytokines in the central nervous system.
  Sredni-Kenigsbuch D. Sredni-Kenigsbuch D. Int J Neurosci. 2002 Jun;112(6):665-703. doi: 10.1080/00207450290025725. Int J Neurosci. 2002. PMID: 12325311 Review.

Cited by

• Without nerves, immunology remains incomplete -in vivo veritas.
  Shepherd AJ, Downing JE, Miyan JA. Shepherd AJ, et al. Immunology. 2005 Oct;116(2):145-63. doi: 10.1111/j.1365-2567.2005.02223.x. Immunology. 2005. PMID: 16162264 Free PMC article. Review.
• beta-2-adrenergic receptor gene polymorphism confers susceptibility to Graves disease.
  Jazdzewski K, Bednarczuk T, Stepnowska M, Liyanarachchi S, Suchecka-Rachon K, Limon J, Narkiewicz K. Jazdzewski K, et al. Int J Mol Med. 2007 Jan;19(1):181-6. Int J Mol Med. 2007. PMID: 17143563 Free PMC article.
• A randomized controlled double-masked trial of albuterol add-on therapy in patients with multiple sclerosis.
  Khoury SJ, Healy BC, Kivisäkk P, Viglietta V, Egorova S, Guttmann CR, Wedgwood JF, Hafler DA, Weiner HL, Buckle G, Cook S, Reddy S. Khoury SJ, et al. Arch Neurol. 2010 Sep;67(9):1055-61. doi: 10.1001/archneurol.2010.222. Arch Neurol. 2010. PMID: 20837847 Free PMC article. Clinical Trial.
• The IL-33-induced p38-/JNK1/2-TNFα axis is antagonized by activation of β-adrenergic-receptors in dendritic cells.
  Helbig C, Weber F, Andreas N, Herdegen T, Gaestel M, Kamradt T, Drube S. Helbig C, et al. Sci Rep. 2020 May 18;10(1):8152. doi: 10.1038/s41598-020-65072-3. Sci Rep. 2020. PMID: 32424229 Free PMC article.
• Corticotropin-releasing hormone deficiency unmasks the proinflammatory effect of epinephrine.
  Karalis KP, Kontopoulos E, Muglia LJ, Majzoub JA. Karalis KP, et al. Proc Natl Acad Sci U S A. 1999 Jun 8;96(12):7093-7. doi: 10.1073/pnas.96.12.7093. Proc Natl Acad Sci U S A. 1999. PMID: 10359844 Free PMC article.

References

1. 1. Eur J Immunol. 1996 Mar;26(3):659-68 - PubMed
2. 1. J Immunol. 1996 Mar 1;156(5):1722-27 - PubMed
3. 1. J Exp Med. 1996 Aug 1;184(2):741-6 - PubMed
4. 1. J Exp Med. 1996 Aug 1;184(2):747-52 - PubMed
5. 1. J Immunol Methods. 1996 Sep 27;196(2):137-51 - PubMed

MeSH terms

Substances

LinkOut - more resources

• Full Text Sources

  • American Society for Clinical Investigation
  • Europe PubMed Central
  • Ovid Technologies, Inc.
  • PubMed Central

• Other Literature Sources

  • The Lens - Patent Citations Database

• Research Materials

  • NCI CPTC Antibody Characterization Program