A minisatellite "core" element constitutes a novel, chromatin-specific activator of mts1 gene transcription - PubMed (original) (raw)
. 1998 Jul 10;280(2):227-36.
doi: 10.1006/jmbi.1998.1857.
Affiliations
- PMID: 9654447
- DOI: 10.1006/jmbi.1998.1857
A minisatellite "core" element constitutes a novel, chromatin-specific activator of mts1 gene transcription
E B Prokhortchouk et al. J Mol Biol. 1998.
Abstract
Expression of the mts1 gene is often associated with malignant transformation of tumor cells. Transcription of the gene is controlled by a number of positive and negative regulatory elements, all of them being localized in the first intron (+38 to +1215) of the mts1 gene. Through analysis of the distribution of DNase I hypersensitive sites in the first intron of the gene we revealed a structurally conserved region that consisted of a non-canonical NFkB binding site and a minisatellite "core" element. Deletion of the minisatellite core DNA in the context of the first intron had no effect on its regulatory capacity when assayed in transient transfections, while a fivefold decrease was observed in a pool of stably transfected cells. The minisatellite core sequence CTGGGCAGGCAG is involved in DNA-protein interactions in vivo, and is similar to a binding site for the previously identified minisatellite DNA sequence binding protein (Msbp-1). The core DNA interacted in vitro with a protein that had an apparent molecular mass of 40 kDa. These data indicate that the minisatellite DNA represents the novel, chromatin-specific element in the mts1 complex enhancer.
Copyright 1998 Academic Press
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