The Saccharomyces cerevisiae RAD9, RAD17, RAD24 and MEC3 genes are required for tolerating irreparable, ultraviolet-induced DNA damage - PubMed (original) (raw)
The Saccharomyces cerevisiae RAD9, RAD17, RAD24 and MEC3 genes are required for tolerating irreparable, ultraviolet-induced DNA damage
A G Paulovich et al. Genetics. 1998 Sep.
Abstract
In wild-type Saccharomyces cerevisiae, a checkpoint slows the rate of progression of an ongoing S phase in response to exposure to a DNA-alkylating agent. Mutations that eliminate S phase regulation also confer sensitivity to alkylating agents, leading us to suggest that, by regulating the S phase rate, cells are either better able to repair or better able to replicate damaged DNA. In this study, we determine the effects of mutations that impair S phase regulation on the ability of excision repair-defective cells to replicate irreparably UV-damaged DNA. We assay survival after UV irradiation, as well as the genetic consequences of replicating a damaged template, namely mutation and sister chromatid exchange induction. We find that RAD9, RAD17, RAD24, and MEC3 are required for UV-induced (although not spontaneous) mutagenesis, and that RAD9 and RAD17 (but not REV3, RAD24, and MEC3) are required for maximal induction of replication-dependent sister chromatid exchange. Therefore, checkpoint genes not only control cell cycle progression in response to damage, but also play a role in accommodating DNA damage during replication.
Similar articles
- RAD9, RAD17, and RAD24 are required for S phase regulation in Saccharomyces cerevisiae in response to DNA damage.
Paulovich AG, Margulies RU, Garvik BM, Hartwell LH. Paulovich AG, et al. Genetics. 1997 Jan;145(1):45-62. doi: 10.1093/genetics/145.1.45. Genetics. 1997. PMID: 9017389 Free PMC article. - G2/M checkpoint genes of Saccharomyces cerevisiae: further evidence for roles in DNA replication and/or repair.
Lydall D, Weinert T. Lydall D, et al. Mol Gen Genet. 1997 Nov;256(6):638-51. doi: 10.1007/s004380050612. Mol Gen Genet. 1997. PMID: 9435789 - Role of PSO genes in repair of DNA damage of Saccharomyces cerevisiae.
Brendel M, Bonatto D, Strauss M, Revers LF, Pungartnik C, Saffi J, Henriques JA. Brendel M, et al. Mutat Res. 2003 Nov;544(2-3):179-93. doi: 10.1016/j.mrrev.2003.06.018. Mutat Res. 2003. PMID: 14644320 Review.
Cited by
- Rad51-mediated replication of damaged templates relies on monoSUMOylated DDK kinase.
Joseph CR, Dusi S, Giannattasio M, Branzei D. Joseph CR, et al. Nat Commun. 2022 May 5;13(1):2480. doi: 10.1038/s41467-022-30215-9. Nat Commun. 2022. PMID: 35513396 Free PMC article. - Non-Recombinogenic Functions of Rad51, BRCA2, and Rad52 in DNA Damage Tolerance.
Prado F. Prado F. Genes (Basel). 2021 Sep 29;12(10):1550. doi: 10.3390/genes12101550. Genes (Basel). 2021. PMID: 34680945 Free PMC article. Review. - Non-recombinogenic roles for Rad52 in translesion synthesis during DNA damage tolerance.
Cano-Linares MI, Yáñez-Vilches A, García-Rodríguez N, Barrientos-Moreno M, González-Prieto R, San-Segundo P, Ulrich HD, Prado F. Cano-Linares MI, et al. EMBO Rep. 2021 Jan 7;22(1):e50410. doi: 10.15252/embr.202050410. Epub 2020 Dec 2. EMBO Rep. 2021. PMID: 33289333 Free PMC article. - Conditional genome engineering reveals canonical and divergent roles for the Hus1 component of the 9-1-1 complex in the maintenance of the plastic genome of Leishmania.
Damasceno JD, Obonaga R, Silva GLA, Reis-Cunha JL, Duncan SM, Bartholomeu DC, Mottram JC, McCulloch R, Tosi LRO. Damasceno JD, et al. Nucleic Acids Res. 2018 Dec 14;46(22):11835-11846. doi: 10.1093/nar/gky1017. Nucleic Acids Res. 2018. PMID: 30380080 Free PMC article. - Coordination of DNA damage tolerance mechanisms with cell cycle progression in fission yeast.
Callegari AJ, Kelly TJ. Callegari AJ, et al. Cell Cycle. 2016;15(2):261-73. doi: 10.1080/15384101.2015.1121353. Cell Cycle. 2016. PMID: 26652183 Free PMC article.
References
- Genetics. 1980 May;95(1):63-80 - PubMed
- Mol Gen Genet. 1997 Nov;256(6):638-51 - PubMed
- Genetics. 1997 Jan;145(1):45-62 - PubMed
- J Bacteriol. 1989 Oct;171(10):5659-67 - PubMed
- Genetics. 1991 May;128(1):79-88 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Research Materials
Miscellaneous