Inhibition of tumor cell growth by RTP/rit42 and its responsiveness to p53 and DNA damage - PubMed (original) (raw)

. 1998 Oct 1;58(19):4439-44.

Affiliations

PMID: 9766676

Inhibition of tumor cell growth by RTP/rit42 and its responsiveness to p53 and DNA damage

S K Kurdistani et al. Cancer Res. 1998.

Abstract

Through a differential screening technique, we have identified a cDNA clone with differential expression in normal versus tumor cells. This clone, designated rit42 (reduced in tumor, 42 kDa), was previously isolated as a homocysteine-inducible gene in human endothelial cells (RTP), and the same or a highly related androgen-responsive gene in mouse has also been identified. Both Northern blot analysis and in situ hybridization demonstrated a significantly diminished expression in tumor cells, including those derived from breast and prostate when compared with normal cells. It was shown that RTP/rit42 mRNA cycles with cell division, peaking at G1 and G2-M, with lower expression in S phase. The biphasic expression of RTP/rit42 mRNA was absent in tumor cells. Introduction of rit42 cDNA into human cancer cells reduced cell growth both in vitro and in nude mice. Moreover, analysis of a tetracycline-regulated p53-inducible system in null-p53 cell lines showed that RTP/rit42 mRNA expression increased concomitantly with p53 expression and followed a similar time course. In addition, DNA-damaging agents induced RTP/rit42 expression in a p53-dependent manner but independent of a p53-mediated G1 arrest. Immunofluorescence analysis of a FLAG epitope-tagged RTP/rit42 protein revealed a cytoplasmic localization pattern with redistribution to the nucleus upon DNA damage. We have localized RTP/rit42 to human chromosome 8q24.3. Taken together, these results are consistent with a growth inhibitory role for RTP/rit42, and its down-regulation may contribute to the tumor malignant phenotype.

PubMed Disclaimer

Cited by

Aberrant NDRG1 methylation associated with its decreased expression and clinicopathological significance in breast cancer.
Han LL, Hou L, Zhou MJ, Ma ZL, Lin DL, Wu L, Ge YL. Han LL, et al. J Biomed Sci. 2013 Jul 30;20(1):52. doi: 10.1186/1423-0127-20-52. J Biomed Sci. 2013. PMID: 23899187 Free PMC article.
Two major branches of anti-cadmium defense in the mouse: MTF-1/metallothioneins and glutathione.
Wimmer U, Wang Y, Georgiev O, Schaffner W. Wimmer U, et al. Nucleic Acids Res. 2005 Oct 12;33(18):5715-27. doi: 10.1093/nar/gki881. Print 2005. Nucleic Acids Res. 2005. PMID: 16221973 Free PMC article.
MicroRNA-769-3p down-regulates NDRG1 and enhances apoptosis in MCF-7 cells during reoxygenation.
Luo EC, Chang YC, Sher YP, Huang WY, Chuang LL, Chiu YC, Tsai MH, Chuang EY, Lai LC. Luo EC, et al. Sci Rep. 2014 Aug 1;4:5908. doi: 10.1038/srep05908. Sci Rep. 2014. PMID: 25081069 Free PMC article.
PI3K-mediated PDGFRα signaling regulates survival and proliferation in skeletal development through p53-dependent intracellular pathways.
Fantauzzo KA, Soriano P. Fantauzzo KA, et al. Genes Dev. 2014 May 1;28(9):1005-17. doi: 10.1101/gad.238709.114. Genes Dev. 2014. PMID: 24788519 Free PMC article.
N-myc downstream-regulated gene 1 inhibits the proliferation and invasion of hepatocellular carcinoma cells via the regulation of integrin β3.
Song Y, Wu G, Zhang M, Kong Q, Du J, Zheng Y, Yue L, Cao L. Song Y, et al. Oncol Lett. 2017 May;13(5):3599-3607. doi: 10.3892/ol.2017.5924. Epub 2017 Mar 27. Oncol Lett. 2017. PMID: 28521460 Free PMC article.

Inhibition of tumor cell growth by RTP/rit42 and its responsiveness to p53 and DNA damage - PubMed (original) (raw)