Mitotic phosphorylation of the TBP-containing factor SL1 represses ribosomal gene transcription - PubMed (original) (raw)

Comparative Study

. 1998 Nov 20;284(1):1-5.

doi: 10.1006/jmbi.1998.2164.

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Comparative Study

Mitotic phosphorylation of the TBP-containing factor SL1 represses ribosomal gene transcription

A Kuhn et al. J Mol Biol. 1998.

Abstract

Entry into mitosis is accompanied by a global repression of transcription. To investigate the molecular mechanisms which shut-down rRNA synthesis during mitosis, we have compared RNA polymerase I (Pol I) transcription in extracts from asynchronous and mitotic HeLa cells. We show by several experimental approaches that phosphorylation by cdc2/cyclin B inactivates the TBP-containing factor SL1 and thus abrogates Pol I transcription during mitosis. This finding links the cell's cycle with the transcriptional activity of Pol I and suggests a common mechanism for mitotic silencing of all three classes of nuclear RNA polymerases, i.e. reversible inactivation of the respective TBP-TAF complexes by (a) mitotic kinase(s).

Copyright 1998 Academic Press

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