Dual function of Ras in Raf activation - PubMed (original) (raw)

. 1998 Dec;125(24):4999-5008.

doi: 10.1242/dev.125.24.4999.

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Dual function of Ras in Raf activation

W Li et al. Development. 1998 Dec.

Abstract

The small guanine nucleotide binding protein p21(Ras) plays an important role in the activation of the Raf kinase. However, the precise mechanism by which Raf is activated remains unclear. It has been proposed that the sole function of p21(Ras )in Raf activation is to recruit Raf to the plasma membrane. We have used Drosophila embryos to examine the mechanism of Raf (Draf) activation in the complete absence of p21(Ras) (Ras1). We demonstrate that the role of Ras1 in Draf activation is not limited to the translocation of Draf to the membrane through a Ras1-Draf association. In addition, Ras1 is essential for the activation of an additional factor which in turn activates Draf.

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