TNF-induced enterocyte apoptosis in mice is mediated by the TNF receptor 1 and does not require p53 - PubMed (original) (raw)
TNF-induced enterocyte apoptosis in mice is mediated by the TNF receptor 1 and does not require p53
P F Piguet et al. Eur J Immunol. 1998 Nov.
Free article
Abstract
Injection of recombinant mouse TNF into mice is known to induce a shrinkage of the duodenal villi, which becomes evident 30-90 min later and is associated with a detachment of enterocytes in the lumen. These cells can be collected by lavage and are all apoptotic, i.e. hypodiploid as seen by flow cytometric analysis. Thus the count of detached cells was used as an evaluation of the TNF-induced cell loss and apoptosis in the mucosa. TNF injection induced a cell loss of similar magnitude in wild-type (+/+) or in mice lacking the TNF receptor (TNFR)2 (p75, TNFR2-/-), while mice lacking the TNFR1 (p55, TNFR1-/-) were completely resistant to this effect. TNF increased the expression of p53 tumor suppressor gene in the enterocytes from the crypts but not from the villi, as seen by Western blots and histochemistry. TNF increased the expression of p53 in both TNFR2-/- and TNFR1-/- mice. Furthermore, enterocyte cell loss was not attenuated in p53-/- mice. The results indicate that TNF, acting on its receptor 1, induces an apoptotic detachment of the enterocytes from the tip of the villi (i.e. the old enterocytes), while in the enterocytes from the crypts (the young enterocytes) TNF increases, via either TNFR1 or TNFR2, the expression of p53, without inducing apoptosis.
Similar articles
- TNF-induced enterocyte apoptosis and detachment in mice: induction of caspases and prevention by a caspase inhibitor, ZVAD-fmk.
Piguet PF, Vesin C, Donati Y, Barazzone C. Piguet PF, et al. Lab Invest. 1999 Apr;79(4):495-500. Lab Invest. 1999. PMID: 10212002 - p53-dependent radiation-induced crypt intestinal epithelial cells apoptosis is mediated in part through TNF-TNFR1 system.
Inagaki-Ohara K, Yada S, Takamura N, Reaves M, Yu X, Liu E, Rooney I, Nicholas S, Castro A, Ware CF, Green DR, Lin T. Inagaki-Ohara K, et al. Oncogene. 2001 Feb 15;20(7):812-8. doi: 10.1038/sj.onc.1204172. Oncogene. 2001. PMID: 11314015 - Tumor necrosis factor (TNF) receptor type 1 (p55) is a main mediator for TNF-alpha-induced skin inflammation.
Kondo S, Sauder DN. Kondo S, et al. Eur J Immunol. 1997 Jul;27(7):1713-8. doi: 10.1002/eji.1830270718. Eur J Immunol. 1997. PMID: 9247582 - Caspase inhibition reveals functional cooperation between p55- and p75-TNF receptors in cell necrosis.
Pelagi M, Curnis F, Colombo B, Rovere P, Sacchi A, Manfredi AA, Corti A. Pelagi M, et al. Eur Cytokine Netw. 2000 Dec;11(4):580-8. Eur Cytokine Netw. 2000. PMID: 11125300 - Role of TNFR1 and TNFR2 in TNF-induced platelet consumption in mice.
Tacchini-Cottier F, Vesin C, Redard M, Buurman W, Piguet PF. Tacchini-Cottier F, et al. J Immunol. 1998 Jun 15;160(12):6182-6. J Immunol. 1998. PMID: 9637537
Cited by
- A hierarchy of cell death pathways confers layered resistance to shigellosis in mice.
Roncaioli JL, Babirye JP, Chavez RA, Liu FL, Turcotte EA, Lee AY, Lesser CF, Vance RE. Roncaioli JL, et al. Elife. 2023 Jan 16;12:e83639. doi: 10.7554/eLife.83639. Elife. 2023. PMID: 36645406 Free PMC article. - Neutrophils and macrophages drive TNF-induced lethality via TRIF/CD14-mediated responses.
Muendlein HI, Connolly WM, Cameron J, Jetton D, Magri Z, Smirnova I, Vannier E, Li X, Martinot AJ, Batorsky R, Poltorak A. Muendlein HI, et al. Sci Immunol. 2022 Dec 23;7(78):eadd0665. doi: 10.1126/sciimmunol.add0665. Epub 2022 Dec 23. Sci Immunol. 2022. PMID: 36563168 Free PMC article. - Group 3 innate lymphoid cells produce the growth factor HB-EGF to protect the intestine from TNF-mediated inflammation.
Zhou L, Zhou W, Joseph AM, Chu C, Putzel GG, Fang B, Teng F, Lyu M, Yano H, Andreasson KI, Mekada E, Eberl G, Sonnenberg GF. Zhou L, et al. Nat Immunol. 2022 Feb;23(2):251-261. doi: 10.1038/s41590-021-01110-0. Epub 2022 Jan 31. Nat Immunol. 2022. PMID: 35102343 Free PMC article. - Epithelium-autonomous NAIP/NLRC4 prevents TNF-driven inflammatory destruction of the gut epithelial barrier in Salmonella-infected mice.
Fattinger SA, Geiser P, Samperio Ventayol P, Di Martino ML, Furter M, Felmy B, Bakkeren E, Hausmann A, Barthel-Scherrer M, Gül E, Hardt WD, Sellin ME. Fattinger SA, et al. Mucosal Immunol. 2021 May;14(3):615-629. doi: 10.1038/s41385-021-00381-y. Epub 2021 Mar 17. Mucosal Immunol. 2021. PMID: 33731826 Free PMC article. - Regulation of inflammatory cytokines for spinal cord injury recovery.
Lin S, Xu C, Lin J, Hu H, Zhang C, Mei X. Lin S, et al. Histol Histopathol. 2021 Feb;36(2):137-142. doi: 10.14670/HH-18-262. Epub 2020 Oct 1. Histol Histopathol. 2021. PMID: 33001420 Review.
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Research Materials
Miscellaneous