Exploiting the basis of proline recognition by SH3 and WW domains: design of N-substituted inhibitors - PubMed (original) (raw)
Exploiting the basis of proline recognition by SH3 and WW domains: design of N-substituted inhibitors
J T Nguyen et al. Science. 1998.
Abstract
Src homology 3 (SH3) and WW protein interaction domains bind specific proline-rich sequences. However, instead of recognizing critical prolines on the basis of side chain shape or rigidity, these domains broadly accepted amide N-substituted residues. Proline is apparently specifically selected in vivo, despite low complementarity, because it is the only endogenous N-substituted amino acid. This discriminatory mechanism explains how these domains achieve specific but low-affinity recognition, a property that is necessary for transient signaling interactions. The mechanism can be exploited: screening a series of ligands in which key prolines were replaced by nonnatural N-substituted residues yielded a ligand that selectively bound the Grb2 SH3 domain with 100 times greater affinity.
Similar articles
- FBP WW domains and the Abl SH3 domain bind to a specific class of proline-rich ligands.
Bedford MT, Chan DC, Leder P. Bedford MT, et al. EMBO J. 1997 May 1;16(9):2376-83. doi: 10.1093/emboj/16.9.2376. EMBO J. 1997. PMID: 9171351 Free PMC article. - Distinct ligand preferences of Src homology 3 domains from Src, Yes, Abl, Cortactin, p53bp2, PLCgamma, Crk, and Grb2.
Sparks AB, Rider JE, Hoffman NG, Fowlkes DM, Quillam LA, Kay BK. Sparks AB, et al. Proc Natl Acad Sci U S A. 1996 Feb 20;93(4):1540-4. doi: 10.1073/pnas.93.4.1540. Proc Natl Acad Sci U S A. 1996. PMID: 8643668 Free PMC article. - Interaction between the amino-terminal SH3 domain of CRK and its natural target proteins.
Matsuda M, Ota S, Tanimura R, Nakamura H, Matuoka K, Takenawa T, Nagashima K, Kurata T. Matsuda M, et al. J Biol Chem. 1996 Jun 14;271(24):14468-72. doi: 10.1074/jbc.271.24.14468. J Biol Chem. 1996. PMID: 8662907 - SH2 and SH3-containing adaptor proteins: redundant or independent mediators of intracellular signal transduction.
Birge RB, Knudsen BS, Besser D, Hanafusa H. Birge RB, et al. Genes Cells. 1996 Jul;1(7):595-613. doi: 10.1046/j.1365-2443.1996.00258.x. Genes Cells. 1996. PMID: 9078388 Review. - Characterization of a novel protein-binding module--the WW domain.
Sudol M, Chen HI, Bougeret C, Einbond A, Bork P. Sudol M, et al. FEBS Lett. 1995 Aug 1;369(1):67-71. doi: 10.1016/0014-5793(95)00550-s. FEBS Lett. 1995. PMID: 7641887 Review.
Cited by
- A Peptoid-Chelator Selective to Cu2+ That Can Extract Copper from Metallothionein-2 and Lead to the Production of ROS.
Behar AE, Maayan G. Behar AE, et al. Antioxidants (Basel). 2023 Nov 22;12(12):2031. doi: 10.3390/antiox12122031. Antioxidants (Basel). 2023. PMID: 38136151 Free PMC article. - Recognition of tandem PxxP motifs as a unique Src homology 3-binding mode triggers pathogen-driven actin assembly.
Aitio O, Hellman M, Kazlauskas A, Vingadassalom DF, Leong JM, Saksela K, Permi P. Aitio O, et al. Proc Natl Acad Sci U S A. 2010 Dec 14;107(50):21743-8. doi: 10.1073/pnas.1010243107. Epub 2010 Nov 22. Proc Natl Acad Sci U S A. 2010. PMID: 21098279 Free PMC article. - Harnessing the PD-L1 interface peptide for positron emission tomography imaging of the PD-1 immune checkpoint.
Hu K, Xie L, Hanyu M, Zhang Y, Li L, Ma X, Nagatsu K, Suzuki H, Wang W, Zhang MR. Hu K, et al. RSC Chem Biol. 2020 Aug 27;1(4):214-224. doi: 10.1039/d0cb00070a. eCollection 2020 Oct 1. RSC Chem Biol. 2020. PMID: 34458761 Free PMC article. - Diverse recognition of non-PxxP peptide ligands by the SH3 domains from p67(phox), Grb2 and Pex13p.
Kami K, Takeya R, Sumimoto H, Kohda D. Kami K, et al. EMBO J. 2002 Aug 15;21(16):4268-76. doi: 10.1093/emboj/cdf428. EMBO J. 2002. PMID: 12169629 Free PMC article. - Efficient production of gamma-aminobutyric acid using Escherichia coli by co-localization of glutamate synthase, glutamate decarboxylase, and GABA transporter.
Dung Pham V, Somasundaram S, Lee SH, Park SJ, Hong SH. Dung Pham V, et al. J Ind Microbiol Biotechnol. 2016 Jan;43(1):79-86. doi: 10.1007/s10295-015-1712-8. Epub 2015 Nov 30. J Ind Microbiol Biotechnol. 2016. PMID: 26620318
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Molecular Biology Databases
Research Materials
Miscellaneous