The role of evolutionarily conserved sequences in alternative splicing at the 3' end of Drosophila melanogaster myosin heavy chain RNA - PubMed (original) (raw)
The role of evolutionarily conserved sequences in alternative splicing at the 3' end of Drosophila melanogaster myosin heavy chain RNA
D Hodges et al. Genetics. 1999 Jan.
Abstract
Exon 18 of the muscle myosin heavy chain gene (Mhc) of Drosophila melanogaster is excluded from larval transcripts but included in most adult transcripts. To identify cis-acting elements regulating this alternative RNA splicing, we sequenced the 3' end of Mhc from the distantly related species D. virilis. Three noncoding regions are conserved: (1) the nonconsensus splice junctions at either end of exon 18; (2) exon 18 itself; and (3) a 30-nucleotide, pyrimidine-rich sequence located about 40 nt upstream of the 3' splice site of exon 18. We generated transgenic flies expressing Mhc mini-genes designed to test the function of these regions. Improvement of both splice sites of adult-specific exon 18 toward the consensus sequence switches the splicing pattern to include exon 18 in all larval transcripts. Thus nonconsensus splice junctions are critical to stage-specific exclusion of this exon. Deletion of nearly all of exon 18 does not affect stage-specific utilization. However, splicing of transcripts lacking the conserved pyrimidine sequence is severely disrupted in adults. Disruption is not rescued by insertion of a different polypyrimidine tract, suggesting that the conserved pyrimidine-rich sequence interacts with tissue-specific splicing factors to activate utilization of the poor splice sites of exon 18 in adult muscle.
Similar articles
- Positive and negative intronic regulatory elements control muscle-specific alternative exon splicing of Drosophila myosin heavy chain transcripts.
Standiford DM, Sun WT, Davis MB, Emerson CP Jr. Standiford DM, et al. Genetics. 2001 Jan;157(1):259-71. doi: 10.1093/genetics/157.1.259. Genetics. 2001. PMID: 11139507 Free PMC article. - Splice-junction elements and intronic sequences regulate alternative splicing of the Drosophila myosin heavy chain gene transcript.
Standiford DM, Davis MB, Sun W, Emerson CP Jr. Standiford DM, et al. Genetics. 1997 Oct;147(2):725-41. doi: 10.1093/genetics/147.2.725. Genetics. 1997. PMID: 9335608 Free PMC article. - Suboptimal 5' and 3' splice sites regulate alternative splicing of Drosophila melanogaster myosin heavy chain transcripts in vitro.
Hodges D, Bernstein SI. Hodges D, et al. Mech Dev. 1992 May;37(3):127-40. doi: 10.1016/0925-4773(92)90075-u. Mech Dev. 1992. PMID: 1498040 - Conserved alternative splicing patterns and splicing signals in the Drosophila sodium channel gene para.
Thackeray JR, Ganetzky B. Thackeray JR, et al. Genetics. 1995 Sep;141(1):203-14. doi: 10.1093/genetics/141.1.203. Genetics. 1995. PMID: 8536968 Free PMC article.
Cited by
- Analysis of mutually exclusive alternatively spliced serpin-1 isoforms and identification of serpin-1 proteinase complexes in Manduca sexta hemolymph.
Ragan EJ, An C, Yang CT, Kanost MR. Ragan EJ, et al. J Biol Chem. 2010 Sep 17;285(38):29642-50. doi: 10.1074/jbc.M110.125419. Epub 2010 Jul 12. J Biol Chem. 2010. PMID: 20624920 Free PMC article. - Positive and negative intronic regulatory elements control muscle-specific alternative exon splicing of Drosophila myosin heavy chain transcripts.
Standiford DM, Sun WT, Davis MB, Emerson CP Jr. Standiford DM, et al. Genetics. 2001 Jan;157(1):259-71. doi: 10.1093/genetics/157.1.259. Genetics. 2001. PMID: 11139507 Free PMC article. - Assembly of thick filaments and myofibrils occurs in the absence of the myosin head.
Cripps RM, Suggs JA, Bernstein SI. Cripps RM, et al. EMBO J. 1999 Apr 1;18(7):1793-804. doi: 10.1093/emboj/18.7.1793. EMBO J. 1999. PMID: 10202143 Free PMC article.
References
- Genetics. 1995 Sep;141(1):203-14 - PubMed
- Biotechniques. 1989 Sep;7(8):798-800 - PubMed
- J Cell Biol. 1995 Dec;131(6 Pt 1):1471-81 - PubMed
- Proc Natl Acad Sci U S A. 1990 Aug;87(15):5916-20 - PubMed
- Genes Dev. 1990 Jun;4(6):885-95 - PubMed
Publication types
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Molecular Biology Databases
Research Materials