Biswash Sapkota | Rajiv Gandhi University of Health Sciences (original) (raw)

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Papers by Biswash Sapkota

Ethnobotany of the Himalayas

American Journal of Psychiatry and Neuroscience

Pharmaceutical Sciences and Research

Journal of Pharmacognosy and Phytochemistry , 2019

Nepalese medicinal plants were selected to study phytochemical presence, antioxidant, anti-inflam... more Nepalese medicinal plants were selected to study phytochemical presence, antioxidant, anti-inflammatory
and analgesic activity of plants. Extraction was carried out by double maceration by ethanol and water.
Phytochemical screening was performed by standard methods. Total phenol content was determined by
Folin Ciocalteu method, flavonoid content was determined by aluminium chloride colorimetric method.
The antioxidant activity was tested by DPPH free radical scavenging assay. In Vivo anti-inflammatory
and analgesic activity was performed by carrageenan induced rat hind paw edema and hot plate methods
respectively. The phytochemical analysis revealed the presence of flavonoids, alkaloids, tannins,
saponins, phenols, terpenoids, glycosides and carbohydrates. The ethanolic and aqueous extract of
Viscum album showed total phenols content, total flavonoids content and has significant antioxidant
activity. Similarly, the aqueous extract of Viscum album has dose dependent analgesic activity. Thus,
medicinal plants possess different compounds with valuable antioxidant properties which can contribute
to the development of modern medicines.

world Journal of Pharmacy and Pharmaceutical Sciences , 2017

Silver is nontoxic, safe inorganic agent and used for management of wounds and ulcer. They have w... more Silver is nontoxic, safe inorganic agent and used for management of wounds and ulcer. They have wide range of application in form of nanoparticles(NPs). Synthesis of silver nanoparticle (AgNPs) through cost effective and environmental friendly methods is of great concern. This review focuses on green synthesis of silver nano-particles from fruits. A detailed study of synthesis of silver nanoparticles from various fruits extracts was demonstrated. Synthesis was observed as a color change of the mixture. The X-ray diffraction patterns, UV-Vis spectrum, Scanning Electron Microscope, Transmission Electron Microscope, Fourier Transform Infrared Spectroscopy, were used for characterization of AgNPs. Antibacterial activitiy of AgNPs was also observed against Gram-positive and Gram-negative bacteria. Various information's of AgNPs synthesis, characterization techniques, properties and their application are summarised and critically discussed in this review.

Objective The objective of the study was to investigate the antioxidant activity of selected medi... more Objective The objective of the study was to investigate the antioxidant activity of selected medicinal plants. Method Collected plant species were subjected to maceration in methanol for 72 hrs. Antioxidant activity of plant extracts was assessed by using DPPH free radical scavenging method in different concentrations (1 µg/ml, 3 µg/ml, 5 µg/ ml, 7 µg/ml and 10 µg/ml) and percentage inhibition and effective concentration (EC50) values were calculated. Result Result indicates that, EC50 of D. boryanum (3.75 µg/ml) and P. guajava (3.89 µg/ml) was less, EC50 of R. nepalensis (5.03 µg/ml) and S. japonica (6.75 µg/ml) was comparable and EC50 M. macrophylla (7.86 µg/ml), B. asiatica (9.14 µg/ml), E. adenophorum (7.78 µg/ml), E. crassipes (8.21 µg/ml), and N. arbortritis (8.16 µg/ml) was slightly higher than ascorbic acid (4.73 µg/ml). Conclusion Our result shows that, D. boryanum and P. gvajava possess higher antioxidant activity than the ascorbic acid implying that, they could be good free radical scavenging agents and should be urbanized as a future pharmaceutical agent.

Awareness regarding H1N1 is important for individual protection and to cease the spread of virus.... more Awareness regarding H1N1 is important for individual protection and to cease the spread of virus. Adequate knowledge, positive attitude and healthy practice of people regarding swine flu are cornerstone in prevention of disease outbreak. The objective of this study was child health promotion by improving knowledge, attitude and practice (KAP) of children. It was a cross sectional study which was conducted during the year 2017/18. A closed ended questionnaire was designed to collect information about social demographic information, knowledge, attitude and behavior of children. Same questionnaire was used in pre and post test, before and 1 month after implementation of the awareness program. Results indicate that there was a remarkable increase in the level of knowledge, attitude and practice subsequent to the accomplishment of the awareness program.

Ethnobotany of the Himalayas

American Journal of Psychiatry and Neuroscience

Pharmaceutical Sciences and Research

Journal of Pharmacognosy and Phytochemistry , 2019

Nepalese medicinal plants were selected to study phytochemical presence, antioxidant, anti-inflam... more Nepalese medicinal plants were selected to study phytochemical presence, antioxidant, anti-inflammatory
and analgesic activity of plants. Extraction was carried out by double maceration by ethanol and water.
Phytochemical screening was performed by standard methods. Total phenol content was determined by
Folin Ciocalteu method, flavonoid content was determined by aluminium chloride colorimetric method.
The antioxidant activity was tested by DPPH free radical scavenging assay. In Vivo anti-inflammatory
and analgesic activity was performed by carrageenan induced rat hind paw edema and hot plate methods
respectively. The phytochemical analysis revealed the presence of flavonoids, alkaloids, tannins,
saponins, phenols, terpenoids, glycosides and carbohydrates. The ethanolic and aqueous extract of
Viscum album showed total phenols content, total flavonoids content and has significant antioxidant
activity. Similarly, the aqueous extract of Viscum album has dose dependent analgesic activity. Thus,
medicinal plants possess different compounds with valuable antioxidant properties which can contribute
to the development of modern medicines.

world Journal of Pharmacy and Pharmaceutical Sciences , 2017

Silver is nontoxic, safe inorganic agent and used for management of wounds and ulcer. They have w... more Silver is nontoxic, safe inorganic agent and used for management of wounds and ulcer. They have wide range of application in form of nanoparticles(NPs). Synthesis of silver nanoparticle (AgNPs) through cost effective and environmental friendly methods is of great concern. This review focuses on green synthesis of silver nano-particles from fruits. A detailed study of synthesis of silver nanoparticles from various fruits extracts was demonstrated. Synthesis was observed as a color change of the mixture. The X-ray diffraction patterns, UV-Vis spectrum, Scanning Electron Microscope, Transmission Electron Microscope, Fourier Transform Infrared Spectroscopy, were used for characterization of AgNPs. Antibacterial activitiy of AgNPs was also observed against Gram-positive and Gram-negative bacteria. Various information's of AgNPs synthesis, characterization techniques, properties and their application are summarised and critically discussed in this review.

Objective The objective of the study was to investigate the antioxidant activity of selected medi... more Objective The objective of the study was to investigate the antioxidant activity of selected medicinal plants. Method Collected plant species were subjected to maceration in methanol for 72 hrs. Antioxidant activity of plant extracts was assessed by using DPPH free radical scavenging method in different concentrations (1 µg/ml, 3 µg/ml, 5 µg/ ml, 7 µg/ml and 10 µg/ml) and percentage inhibition and effective concentration (EC50) values were calculated. Result Result indicates that, EC50 of D. boryanum (3.75 µg/ml) and P. guajava (3.89 µg/ml) was less, EC50 of R. nepalensis (5.03 µg/ml) and S. japonica (6.75 µg/ml) was comparable and EC50 M. macrophylla (7.86 µg/ml), B. asiatica (9.14 µg/ml), E. adenophorum (7.78 µg/ml), E. crassipes (8.21 µg/ml), and N. arbortritis (8.16 µg/ml) was slightly higher than ascorbic acid (4.73 µg/ml). Conclusion Our result shows that, D. boryanum and P. gvajava possess higher antioxidant activity than the ascorbic acid implying that, they could be good free radical scavenging agents and should be urbanized as a future pharmaceutical agent.

Awareness regarding H1N1 is important for individual protection and to cease the spread of virus.... more Awareness regarding H1N1 is important for individual protection and to cease the spread of virus. Adequate knowledge, positive attitude and healthy practice of people regarding swine flu are cornerstone in prevention of disease outbreak. The objective of this study was child health promotion by improving knowledge, attitude and practice (KAP) of children. It was a cross sectional study which was conducted during the year 2017/18. A closed ended questionnaire was designed to collect information about social demographic information, knowledge, attitude and behavior of children. Same questionnaire was used in pre and post test, before and 1 month after implementation of the awareness program. Results indicate that there was a remarkable increase in the level of knowledge, attitude and practice subsequent to the accomplishment of the awareness program.