Vishnu Kumar | Dr. Ram Manohar Lohiya Avadh University Faizabad (original) (raw)

Papers by Vishnu Kumar

book chapter, 2017

Antidiabetic, antidyslipoproteinemic and antioxidant activities of Anthocephalus indicus; KADAM, ... more Antidiabetic, antidyslipoproteinemic and antioxidant activities of Anthocephalus indicus; KADAM, roots and fruits, Hibiscus rosa sinensis roots, Tinospora cordifolia stem and Cassia tora seeds in Triton, High Fat Diet (HFD), Alloxan and Streptozotocin (STZ) model of dyslipoproteinemia were studied. In vitro studies were also carried out to explore antioxidant activities of all above plants in vitro. Clinical trial was carried out for T. cordifolia only. In experimental models of dyslipoproteinemia values of Cholic acid and Deoxycholic acid were decreased in feces of rats. While value of Super oxide dismutase, Catalase, Lipoprotein lipase, Triglyceride lipase and Lecithin cholesterol acyl transferase were found decreased in liver homogenate and serum. In Alloxan and Streptozotocin model marked increase in plasma level of glucose and lipid peroxide accompanied with an elevation in the lipid and apoprotein levels of lipoproteins following decrease in lipid and protein constituents of High Density Lipoproteins were observed. However, oral administration of natural products (500 mg/ kg body weight per oral) for one month reversed these levels. To the best of activities of Hibiscus roots and Anthocephalus indicus roots and fruits.

Abstract The present study was undertaken to evaluate antidiabetic and antioxidant activities of ... more Abstract The present study was undertaken to evaluate
antidiabetic and antioxidant activities of Cassia tora (C.
tora) seeds extract against streptozotocin induced diabetes
in experimental rats to scientifically validate its use against
diabetes. Ethanolic extract of C. tora seeds extract and
standard drug (glibenclamide) prepared in aqueous gum
acacia (2 %, w/v) suspension and fed orally to streptozotocin
induced male adult diabetic rats of Charles Foster
strain for 15 days. Biochemical parameters in normal,
diabetic control, standard (600 lg/kg bw p.o.) and treated
(500 mg/kg bw p.o.) animal groups were quantified and
compared. Treatment of streptozotocin induced diabetic
rats with ethanolic seeds extract caused significant
(p\0.001) reduction in blood glucose (270–220 mg/dl),
total cholesterol (140–104 mg/dl), triglyceride
(149–99 mg/dl), phospholipids (100–74 mg/dl), free fatty
acid (2.39–2.00 lmol/l), lipid peroxide (9–5.63 nmol
MDA/dl) and significantly increased post heparin lipolytic
activity (11–14 nmol FFA released/h/l plasma)
(p\0.001). Furthermore, the seeds extract (100–400 lg)
when tested for its antioxidant activity in vitro, showed
significant (p\0.001) inhibition in the generation of super
oxide anions in enzymic system a (46–37, 33, 23, 21 nmol
uric acid formed/min), in enzymic system b (113–91, 77,
60, 51 nmol formazon formed/min), non-enzymic system
(324–230, 211, 161, 141 nmol uric acid formed/min) and
hydroxyl radicals in enzymic system (544–501, 411, 319,
291 nmol 2,3-dihydroxybenzoate formed/h) and non-enzymic
system (28–21, 17, 14, 12). The results of the present
study demonstrated antidiabetic, antidyslipidemic and
antioxidant activities of C. tora seeds which could help in
prevention of diabeticdyslipidemia and related
complications.
Keywords Cassia tora seeds extract Anti-dyslipidemic
agent Post heparin lipolytic activity Streptozotocin
induced diabetes Plant antioxidant

The hypolipidemic activity of Cassia tora (Chakvat, Chakunda) (Family: Caesalpiniaceae) seeds ext... more The hypolipidemic activity of Cassia tora (Chakvat, Chakunda) (Family: Caesalpiniaceae) seeds extract have been studied in alloxan-induced diabetic rats. In this model oral administration of seed extract (500 mg/kg bw.P.o.) for 15 days in diabetic-dyslipidemic rats exerted significant lipid lowering effect as assessed by the reversal of serum levels of total cholesterol, phospholipids, triglyceride, free fatty acids, protein and lipid peroxide. The decrease of lipids and apoprotein levels of Very Low Density Lipoprotein and Low Density Lipoprotein were followed by stimulation of plasma post-heparin lipolytic activity as well as lecithin cholesterol acyltransferase, hepatic superoxide dismutase, catalase, triglyceride lipase and lipoprotein lipase activities with increase in reduced glutathione. Lipid and apoprotein level of High Density Lipoprotein were also recovered partially on treatment with seeds extract. The results of the present study demonstrated antidyslipidemic and antioxidant activities in seed extract of C. tora which could be used in prevention of diabetic dyslipidemia and related complications. The hypolipidemic activity of Cassia tora seeds was compared with a standard drug glibenclamide (600 µg/ kg body wt/ day p.o.).

The present study was carried out to explore anti-diabetic, antidyslipoproteinemic and anti oxida... more The present study was carried out to explore anti-diabetic, antidyslipoproteinemic and anti oxidant activities of Tinospora cordifolia (Menispermaceae) in type 2 diabetic patients. In this study clinical trial was conducted on type 2 diabetic patients attending the diabetes OPD, at Kaya Chikitsha Vibhag, Rajkiya Ayurvedic Chikitsha Mahavidyalaya, Touriya Ganj, Lucknow, following all ethical guide lines. All biochemical assays were carried out by the standard kit methods. A marked increase in plasma levels of fasting blood sugar, lipid profile accompanied with increase in the lipids and apo-protein levels of serum β lipoproteins following decrease in lipid and protein constituents of α lipoprotein were noted in type 2 diabetic patients compared to healthy controls. The results after prescription of Tinospora cordifoia (powered stem; 50 mg/kg body weight, p. o.) for 15 days in type 2 diabetic patients resulted significant decrease in the level of fasting blood sugar, total cholesterol, β lipoproteins and triglycerides. The decrease in lipids and apoprotein levels of β lipoproteins was accompanied with stimulation of plasma lecithin cholesterol acyltransferase (LCAT). Lipid and apoprotein level of α lipoproteins were also recovered partially. Results of this study may provide scope for further research and leading to development of a potent anti diabetic, antidyslipoproteinemic anti oxidant drug from Tinospora cordifolia (T. cordifolia) stem.

Abstract Diabetes mellitus is a group of metabolic disorder characterized by hyperglycemia with g... more Abstract
Diabetes mellitus is a group of metabolic disorder characterized by hyperglycemia with glycosuria and it is well documented that increased level of blood glucose is a marker of disorder of carbohydrate metabolism and is associated with the initiation of diabetic dyslipoproteinemia and other complications. Prolonged free radical mediated lipotoxicity and abnormal glucose tolerance are involved in the pathogenesis of diabetes mellitus. Diabetic dyslipoproteinemia is characterized by the increased level of cholesterol, reduced HDL and high triglyceride (TG) levels.The present study was carried out to explore the status of oxidative stress, antioxidant enzymes, lipids and lipoprotein profile in type-2 diabetic patients. This study was conducted on type 2 diabetic patients attending the diabetes OPD, Era’s Lucknow Medical & Hospital, Sarfaraz Ganj, Hardoi Road, Lucknow. All biochemical assays were carried out by the standard kit methods. A marked increase in plasma levels of fasting blood sugar, lipid peroxide, lipid profile accompanied with increase in the lipids and apo-protein levels of serum β lipoproteins following decrease in lipid and protein constituents of α lipoprotein, antioxidant enzymes and reduced glutathione were noted in type 2 diabetic patients compared to healthy controls. Type 2 diabetic patients are consistently associated with disorder of metabolism and disturb redox status.

Key words: Lipid profile-Lipoprotein profile-Oxidative stress-Antioxidants-LCAT.

Abstract Hyperglycemia, oxidative stress and dyslipoproteinemia are well known factors for Coron... more Abstract

Hyperglycemia, oxidative stress and dyslipoproteinemia are well known factors for Coronary artery disease (CAD) which is the major cause of mortality and morbidity worldwide. The incident of CAD with type 2 diabetes mellitus (T2DM) is rising and they are predicted to be the biggest cause of death by 2020 in India. Therefore, the aim of the present study was to assess the association of hyperglycemia, oxidative stress, antioxidant enzymes, lipid profile and lipoprotein sub fractions in CAD with T2DM patients as well as to compare the results with age-sex matched healthy human volunteers. Three hundred participants were enrolled for the present study, with their ages ranging from 45 to 55 years from Era’s Lucknow Medical College & Hospital, Lucknow. Out of which one hundred fifty were clinically new diagnosed case of CAD with T2DM like hyperglycemia, angina pectoris and Myocardial infarction (MI), remaining one hundred fifty were healthy controls. All biochemical assays were carried out by the standard kit methods. These participants were investigated for serum lipid profile, Lipoprotein sub fractions along with blood levels of lipid peroxide, reduced glutathione and antioxidant enzymes. A marked impairment in plasma levels of lipid profile accompanied with increase in the lipids and apo-protein levels of serum β lipoproteins following decrease in lipid and protein constituents of α lipoprotein, reduced glutathione as well as level of antioxidant enzymes were noted in CAD with T2DM patients with respected to healthy controls.

Abstract The antidyslipidemic activities of Tinospora cordifolia (T. cordifolia): family Menisper... more Abstract The antidyslipidemic activities of Tinospora
cordifolia (T. cordifolia): family Menispermaceae, Hindi
name: Giloya; Gurch or Tinospora in english, extract have
been studied in alloxan induced diabetic rats. In this model,
oral administration of stem extract (500 mg/kg bw. p.o.)
for 30 days resulted significant decreased in the levels of
blood glucose, plasma lipids and reactivated post heparin
lipoprotein lipase activity in alloxan induced diabetic rats.
Furthermore, the stem extract (50–500 lg) when tested for
its antioxidant activity, shown to inhibit the generation of
super oxide anions and hydroxyl radicals, in both enzymic
and non-enzymic systems in vitro. The results of the present
study demonstrated antidyslipidemic and antioxidant
activities of T. cordifolia which could help in prevention of
diabetic-dyslipidemia and related complications.
Keywords Tinospora cordifolia Anti-dyslipidemic
agent Natural antioxidants Post heparin lipolytic
activity Alloxan induced-diabetes Hypoglycemic agent

QUALITY MANUAL HOSPITAL LABORATORY SERVICES, ERA’S LUCKNOW MEDICAL COLLEGE & HOSPITAL (ELMC&H)... more QUALITY MANUAL

HOSPITAL LABORATORY SERVICES,
ERA’S LUCKNOW MEDICAL COLLEGE & HOSPITAL (ELMC&H),
SARFARAZ GANJ, HARDOI ROAD, LUCKNOW – 226003

DOCUMENT NO. : NABL 160
ISSUE DATE : dd/mm /yy
COPY NO. :
ISSUED BY : Dr…………………………
HOLDER’S NAME :
QUALITY MANAGER : DR VISHNU KUMAR

HOSPITAL LABORATORY SERVICES, ERA’S LUCKNOW MEDICAL COLLEGE & HOSDPITAL, SARFARAZ GANJ, HARDOI R... more HOSPITAL LABORATORY SERVICES,
ERA’S LUCKNOW MEDICAL COLLEGE & HOSDPITAL, SARFARAZ GANJ, HARDOI ROAD, LUCKNOW - 226003

QUALITY MANUAL
(Written by Dr Vishnu Kumar)

ABSTRACT: The hypolipidemic activity of Cassia tora (Chakvat, Chakunda) (Family: Caesalpiniaceae)... more ABSTRACT:
The hypolipidemic activity of Cassia tora (Chakvat, Chakunda) (Family: Caesalpiniaceae) seeds extract have been studied in alloxan-induced diabetic rats. In this model oral administration of seed extract (500 mg/kg bw. P. o.) for 15 days in diabetic-dyslipidemic rats exerted significant lipid lowering effect as assessed by the reversal of serum levels of total cholesterol, phospholipids, triglyceride, free fatty acids, protein and lipid peroxide. The decrease of lipids and apoprotein levels of Very Low Density Lipoprotein and Low Density Lipoprotein were followed by stimulation of plasma post-heparin lipolytic activity as well as lecithin cholesterol acyltransferase, hepatic superoxide dismutase, catalase, triglyceride lipase and lipoprotein lipase activities with increase in reduced glutathione. Lipid and apoprotein level of High Density Lipoprotein were also recovered partially on treatment with seeds extract. The results of the present study demonstrated antidyslipidemic and antioxidant activities in seed extract of C. tora which could be used in prevention of diabetic dyslipidemia and related complications. The hypolipidemic activity of Cassia tora seeds was compared with a standard drug glibenclamide (600 µg/ kg body wt/ day p.o.).

KEYWORDS: Alloxan model of hyperglycemia – Cholic and Deoxycholic acid – Antioxidant enzymes - Lipases - Lecithin cholesterol acyltransferase – Plant antioxidants.

Quality Manual is controlled document of HLS, while NABL ISO 15189 copy of Quality Manual is unco... more Quality Manual is controlled document of HLS, while NABL ISO 15189 copy of Quality Manual is uncontrolled document. This is First Version of Quality Manual written by DR VISHNU KUMAR AWASTHI, ASSISTANT PROFESSOR - IN - CHARGE HLS.

ABSTRACT The antidyslipidemic activity of Tinospora cordifolia (T. cordifolia): family Menisperma... more ABSTRACT
The antidyslipidemic activity of Tinospora cordifolia (T. cordifolia): family Menispermaceae, Hindi name: Giloya; Gurch or Tinospora in english, extract has been studied in alloxan induced diabetic rats. In this model, oral administration of stem extract (500 mg/kg bw. p.o.) for 30 days resulted significant decreased in the levels of blood glucose, plasma lipids and reactivated post heparin lipoprotein lipase activity in alloxan induced diabetic rats. Furthermore, the stem extract (50-500 µg) when tested for its antioxidant activity, shown to inhibit the generation of super oxide anions and hydroxyl radicals, in both enzymic and non-enzymic systems in vitro. The results of the present study demonstrated antidyslipidemic and antioxidant activities of Tinospora cordifolia which could help in prevention of diabetic- dyslipidemia and related complications.

KEY WORDS
Tinospora cordifolia, Anti-dyslipidemic agent, Natural Antioxidants, Post heparin lipolytic activity, Alloxan induced-diabetes. Hypoglycemic agent.

The lipid lowering activity of Anthocephalus indicus (family Rubiaceae; Hindi name Kadamba) root ... more The lipid lowering activity of Anthocephalus indicus (family Rubiaceae; Hindi name Kadamba) root extract has been studied in triton WR-1339 induced hyperlipidemia in rats. In this model, feeding with root extract (500 mg kg À1 b.w.) lowered plasma lipids and reactivated post-heparin lipolytic activity in hyperlipidemic rats. Furthermore, the root extract (50-500 mM) inhibited the generation of superoxide anions and hydroxyl radicals, in both enzymic and non-enzymic systems, in vitro. The results of the present study demonstrated both lipid lowering and antioxidant activities in root extract of A. indicus, which could help prevention of hyperlipidemia and related diseases.

The hypolipidemic action of Anthocephalus indicus (family, Rubiaceae: Hindi name, Kadam) fruit ex... more The hypolipidemic action of Anthocephalus indicus (family, Rubiaceae: Hindi name, Kadam) fruit extract has been studied in hyperlipidemic rats fed a triton-and cholesterol-rich high-fat diet. In triton WR-1339-induced hyperlipidemic rats, feeding with the fruit extract (500 mg/kg b.w.) exerted a lipid-lowering effect as assessed by reversal of plasma levels of total cholesterol, phospholipids, and triglyceride following reactivation of the post-heparin lipolytic activity. In another model, chronic feeding of this natural product (500 mg/kg, b.w.) to animals simultaneously fed a high-fat diet for 30 days caused lowering of lipid levels in plasma and liver accompanied with stimulation of hepatic lipolytic activity. The hypolipidemic activity of Anthocephalus indicus fruit extract iscompared with guggulipid, a known lipid-lowering drug, in both models.

The present study was carried out to investigate the antidyslipidemic activity of Tinospora cordi... more The present study was carried out to investigate the antidyslipidemic activity of Tinospora cordifolia stem extract in alloxan-induced (150 mg/kg body wt.) diabetic rats. There was significant increase in plasma markers of diabeticdyslipidemia namely: the levels of glucose, glycosylated hemoglobin(Hb A1c ) lipid peroxide, total lipid and free fatty acids (FFA).The abnormalities with lipid metabolism was .accompanied with increase in the lipids and apoprotein levels of serum very low density lipoprotein (VLDL) and low density lipoprotein (LDL) following decrease in high density lipoprotein (HDL) and diminution of plasma lecithin cholesterol acyltransferase(LCAT ) and post-heparin lipolytic activity (PHLA ).The alteration in lipoprotein pattern was associated with inhibition of hepatic lipolytic (LPL) and antioxidant enzymes; superoxide desmutase(SOD) and catalase as well as decreased excretion of bile acids through feces of diabetic rats. Oral administration of Tinospora cordifolia stem extract (500 mg/kg body weight) for 15 days in dyslipidemic animals resulted in significant decrease in plasma glucose, Hb A1c lipid peroxide, total lipid and FFA. The decrease in the plasma level of lipid peroxide and lipid and apoprotein components of VLDL and LDL were also accompanied by stimulation of plasma PHLA, LCAT and hepatic LPL, SOD and catalase activities. Lipid and apoprotein level of HDL were also recovered partially by the treatment with root extract. Although anti diabetic effect of T. cordifolia was less than glibenclamide, however, its anti dyslipidemic activity was more pronounced in above test model .The result of present study leads to research and development of a potent antidyslipidemic drug from Tinospora cordifolia.

book chapter, 2017

Key words: Lipid profile-Lipoprotein profile-Oxidative stress-Antioxidants-LCAT.

Abstract Hyperglycemia, oxidative stress and dyslipoproteinemia are well known factors for Coron... more Abstract

QUALITY MANUAL HOSPITAL LABORATORY SERVICES, ERA’S LUCKNOW MEDICAL COLLEGE & HOSPITAL (ELMC&H)... more QUALITY MANUAL

HOSPITAL LABORATORY SERVICES,
ERA’S LUCKNOW MEDICAL COLLEGE & HOSPITAL (ELMC&H),
SARFARAZ GANJ, HARDOI ROAD, LUCKNOW – 226003

DOCUMENT NO. : NABL 160
ISSUE DATE : dd/mm /yy
COPY NO. :
ISSUED BY : Dr…………………………
HOLDER’S NAME :
QUALITY MANAGER : DR VISHNU KUMAR

QUALITY MANUAL
(Written by Dr Vishnu Kumar)

KEYWORDS: Alloxan model of hyperglycemia – Cholic and Deoxycholic acid – Antioxidant enzymes - Lipases - Lecithin cholesterol acyltransferase – Plant antioxidants.

KEY WORDS
Tinospora cordifolia, Anti-dyslipidemic agent, Natural Antioxidants, Post heparin lipolytic activity, Alloxan induced-diabetes. Hypoglycemic agent.

Formerly King George's Medical University), Lucknow. His vast ocean of knowledge, exceptional exp... more Formerly King George's Medical University), Lucknow. His vast ocean of knowledge, exceptional expression power and his zeal for the subject has earned him international recognition. He has been a constant source of inspiration for me to strive for perfection. His affectionate behavior, fatherly control, selfless and sympathetic attitude means a lot to me. I am highly indebted to him for all the efforts and pains taken by him in not only seeing this work through but also inculcating in me the zeal to become a good human being and a good scholar. His academic flair constantly reminds me the famous words of Jacques Barzan: "The test and the use of man's education is that he finds pleasure in exercise of his mind".

The present study was designed, to explore the hypoglycemic, antioxidant and lipid lowering activ... more The present study was designed, to explore the hypoglycemic, antioxidant and lipid lowering activities in few medicinal plants on diabetic models.

With deep sense of gratitude and indebtedness I express my reverence to my esteemed suprevisor Dr... more With deep sense of gratitude and indebtedness I express my reverence to my esteemed suprevisor Dr. (Mrs.) Ranjana Singh, Senior Demonstrator, Department of Biochemistry, King George's Medical College, Lucknow. Her vast ocean of knowledge, exceptional expression power and her zeal for the subject has earned her international recognition. She has been a constant source of inspiration for me to strive for perfection. Her affectionate behavior, selfless and sympathetic attitude means a lot to me. I am highly indebted to her for all the efforts and pains taken by her in not only seeing this work through but also inculcating in me the zeal to become a good human being and a good scholar.