Dr. Souravh Bais | SAGE (original) (raw)

Papers by Dr. Souravh Bais

Pharmaceutica Analytica Acta, Apr 20, 2015

INNOSC theranostics & pharmacological sciences, Feb 3, 2023

The aim of the present study is to formulate and evaluate a polyherbal gel that contains Curcuma ... more The aim of the present study is to formulate and evaluate a polyherbal gel that contains Curcuma longa extract. The ethanolic extract of C. longa's rhizome was used to create a gel formulation in different concentrations (1, 2, 3, and 4%). Topical anti-inflammatory activity of gel was also assessed. Gel was prepared using Carbopol ® 940 (1% w/v), C. longa extract, ethanol, propylene glycol 400, methyl paraben, propyl paraben, ethylenediaminetetraacetic acid, tri-ethanolamine, and the necessary amount of distilled water. The prepared formulations were assessed for their physical qualities, pH, spreadability, and ability to irritate skin to detect toxicity or side effects. The findings suggested that gel compositions were good in terms of appearance and uniformity.

International journal of pharma and bio sciences, Oct 11, 2018

International Journal of Green Pharmacy, 2013

Background: Amorphophallus companulatus (Araceae) is a tuberous medicinal plant commonly used in ... more Background: Amorphophallus companulatus (Araceae) is a tuberous medicinal plant commonly used in Ayuvedic medicines as well as tribal medicines of India. Aim: Aim of the present study was to investigate the protective effect of A. companulatus tuber extracts against H 2 O 2 induced oxidative damage in human erythrocytes and leucocytes. Setting and Design: The experiment was set and design as per available method in the literatures. Three measurements were performed under each set of extracts. Materials and Methods: The extracts of tuber of A. companulatus such as methanol, ethanol, acetone (70%) and hydro-alcohol (1:1) used to assess catalase (CAT), superoxide dismutase (SOD), glutathione peroxidase (GPx), glutathione (GSH) and lipid peroxidation (LPO) levels of human erythrocytes and leucocytes. Statistical Analysis: All experimental data were statistically analysed and expressed as means ± SD by using the one-way analysis of variance. Results: Results of present studies revealed that, increased in the CAT, SOD, GPx and reduction of GSH and LPO levels in H 2 O 2 group compared with control group. The extracts of tuber of A. companulatus treated groups showed effective reduction of CAT, SOD, GPx and increased the GSH and LPO levels as compared with H 2 O 2 group on human erythrocytes and leucocytes. The methanol extract was found more effective than others. Conclusion: The present findings suggest that, the extracts of tuber of A. companulatus possess protective effect against H 2 O 2 induced oxidative damage. Furthermore, these tuber extracts may appeared to be beneficial in preventing H 2 O 2 oxidative human red blood cell (RBC) damage in human and can improve RBC membrane permanence. The tubers of A. companulatus are the potential source of natural antioxidants for the treatment and prevention of disease in which LPO takes place.

Obesity Medicine, Jun 1, 2020

In vitro anti diabetic and anti obesity effect of J. Communis Extract on 3T3L1 mouse adipocytes: ... more In vitro anti diabetic and anti obesity effect of J. Communis Extract on 3T3L1 mouse adipocytes: a possible role of MAPK/ERK activation

Research Journal of Phytochemistry, Feb 1, 2015

The aim of the present study was to investigate the protective effects of amentoflavone (AF), iso... more The aim of the present study was to investigate the protective effects of amentoflavone (AF), isolated from six species of Juniperus (AF1-AF6) (Cupressaceae) Juniperus communis L. (AF1), Juniperus wallichiana L. (AF2), Juniperus indica L. (AF3), Juniperus macropoda L. (AF4), juniperus recurva L. (AF5) and Juniperus polycarpos L. (AF6)] against H 2 O 2 induced oxidative damage in human erythrocytes and leucocytes. The experiment was set and designed as per method available in literature. Amentoflavone was isolated from aerial parts of Juniperus species (above six) and authenticated by TLC, IR, HPLC and physicochemical parameters. The two concentration of isolated AF (A = 250 µL, B = 500 µL) were used to assessed its protective effects against H 2 O 2 induced oxidative damage in human erythrocytes and leucocytes. The measurements of different antioxidant parameters like, CAT, SOD and GPx, LPO and GSH levels were performed under each set extracts. Juniperus communis L. (AF1 B) (500 µL) and Juniperus indica L. (AF3 B) (500 µL) were the most significant one on CAT (28.35±2.980 and 27.73±1.580 AU gG 1 Hb), GPx (128.53±3.10 and 41.7±1.420 µg gG 1 Hb) and SOD (1193.5±19.7 and 1532.6±17.60 U gG 1 Hb) enzyme systems of erythrocytes. Among plant extracts, Juniperus communis L. (AF1 B) showed the highest activities on CAT (13.79±1.832 AU mgG 1 protein) and SOD (820±14.50 U mgG 1 protein) while Juniperus indica L. (AF3 B) showed significant effects on Gpx (152.72±3.70 U mgG 1 protein), GSH (25.93±1.560 µg mgG 1 protein) and LPO (10.54±2.90 nmol mgG 1 protein) enzyme systems of leucocytes. The study concludes that isolated fractions of AF from Juniperus species (among six species), has a potential source of natural antioxidants for treatment and prevention of diseases in which oxidative stress takes place.

Ancient Science of Life, 2014

The seeds of Macrotyloma uniflorum Lam. (Family Fabaceae) contain extractable total and tannins w... more The seeds of Macrotyloma uniflorum Lam. (Family Fabaceae) contain extractable total and tannins with reported hepatoprotective, hypocholesterolemic and antioxidant activity. In this study, dietary M. uniflorum seed, methanolic and acetone extracts (ME and AE) were examined for their bile-antilithogenic potential. Mice fed with 1% cholesterol and 0.5% cholic acid lithogenic (LG) diet for 8 weeks resulted in cholesterol super saturation in gallbladder bile, which promotes the formation of cholesterol gallstones (CGSs). AE reduced the CGS incidence by 60.21%, and serum total cholesterol, triglyceride (TG), very low density lipoprotein (LDL) and LDL compared to control animals. Seed extracts at 300 mg/kg dose markedly reduced biliary cholesterol (BC) and decreased bile salt content. The ratio of BC to phospholipid which was 2.64 in the LG diet group was reduced to 1.57-1.35 in the M. uniflorum seed extracts treated groups. Liver cholesterol and TG were decreased significantly by feeding of ME and AE at 300 mg/kg dose. AE significantly reversed the changes in apolipoproteins A-I and C-II level disturbed by LG diet. M. uniflorum seed exerted antilithogenic influence by decreasing the cholesterol hyper-secretion into bile and increasing the bile acid output, thus decreasing the formation of LG bile in mice. The effect was maximum in the AE as it also reduced papillary proliferation of gallbladder and fatty degeneration of the liver. The potential antilithogenic effect of the AE of M. uniflorum may be due to antioxidant property of its rich total polyphenol and tannins content.

International Journal of Pharmacology, Aug 1, 2018

Obesity Medicine, Dec 1, 2019

Background: Adiponectin is a protein hormone secreted by adipocytes. Aim: The aim of present revi... more Background: Adiponectin is a protein hormone secreted by adipocytes. Aim: The aim of present review is to enlist them all, and mention their significance in obesity, which helps researchers in drug discovery and future medicine. Material and methods: The last 20 years data has been searched and studied to mention their current role in obesity treatment and ailments of disease. Results and conclusion: Adiponectin exert its action through receptors AdipoR1, AdipoR2, T-cadherin. In muscle AdipoR1 is exhibit, while in liver AdipoR2 is mainly exhibit. AMPK and PPARα pathway in liver and skeletal muscle are magnified by adiponectin. Adiponectin show many function like expand fatty acids oxidation, control glucose level and manage receptor activity. Circulating level of adiponectin is lower in case of obesity, insulin resistance and other metabolic disorder. In this review we will focus on role of adiponectin and its receptors in metabolic disorder obesity.

Journal of applied pharmaceutical research, Apr 29, 2017

Journal of Addiction Research and Therapy, Aug 13, 2013

P atients who are seen in clinical practice commonly have multiple problems, yet efficacy data of... more P atients who are seen in clinical practice commonly have multiple problems, yet efficacy data often reflect treatment of a single illness. Thus, it is useful to know how standard treatment approaches need to be modified for comorbid disorders. This article briefly describes prevalence, assessment, clinical features, and treatment of comorbid major depression and alcohol dependence. Comorbid is characterised by symptoms of both depression and anxiety. Patients who have comorbid depression and anxiety have higher severity of illness, higher chronicity and significantly greater impairment in work functioning, psychosocial functioning and quality of life than patients only suffering from single disease like depression or anxiety. One of the most important clinical reasons to screen for comorbidity is that unrecognized depression/anxiety comorbidity is associated with an increased rate of psychiatric hospitalization and an increased rate of suicide attempts. Sometimes physicians are not exactly aware of the comorbidity because the symptoms of depression and anxiety are common pathophysiologically. Both major depression and alcohol dependence carry a significant risk for the development of the other. Severity in one disorder is associated with severity in the other. Moreover, alcohol dependence prolongs the course of depression, and persistent depression during abstinence from alcohol is a risk factor for relapse to heavy drinking. Thus, logic dictates that both disorders be identified and managed concurrently and aggressively. Integrated psychosocial outpatient treatment programs and the ability to treat alcohol and depression simultaneously have reinforced the need to revisit the traditional management of comorbid major depression and alcohol dependence more formally.

Human & Experimental Toxicology, 2022

Background Dietary quinic acid given as the nutritional supplement, which may leads to tryptophan... more Background Dietary quinic acid given as the nutritional supplement, which may leads to tryptophan and nicotinamide production in the intestinal tract and NAD+ precursor which can prevent from the negative consequences of high fat diet (HFD) consumption. Objective The present study was designed to assess in vivo and in vitro effect of D-(−)-Quinic acid in high-fat diet induced hyperlipidemia in mice. Material and methods Thirty six albino mice were randomly divided in six groups and each group had six mice. Group I, controlled mice given normal pellet diet, Group-II mice, administered with high fat diet (HFD), Group-III mice given standard drug, Atorvastatin (20 mg/kg, p.o.) along with HFD to mice and Group IV, V and VI mice received D-(−)-Quinic acid at a dose of 75, 150 and 300 mg/kg, respectively in separate group along with HFD to mice. After completion of trial (49 days) the animals were sacrificed and evaluated for body weight, organ fat pad weight, and changes in weight of liver, heart and kidney and also for biochemical parameters, expression of adipogenic and inflammation markers in adipose tissues, and histology examination of liver tissue. Results In vitro testing results showed, D-(−)-Quinic acid potentially inhibit α-glucosidase enzyme activity as compared to acarbose. The D-(−)-Quinic acid showed significant hypolipidemic activity by decreasing the increased level of cholesterol, triglyceride level, LDL, VLDL and other hepatic parameters like SGOT and SGPT in serum. D-(−)-Quinic acid reduces the mRNA expression level of PPAR-γ2, TNF-α, IL-1β and IL-6 in adipose tissue in hyperlipidemic mice.

Obesity Medicine, Dec 1, 2020

Abstract Background gallstone disease or cholelithiasis is a disease caused by change in bile sec... more Abstract Background gallstone disease or cholelithiasis is a disease caused by change in bile secretions and cholesterol saturation to form crystal and blockage in bile duct. Aim the aim of present study evaluate protective effect of Juniperus Communis extract by inhibition of Pro-nucleating agents in lithogenic diet induced cholelithiasis in mice. Material and methods Mice fed with diet containing 1% cholesterol and 0.5% for six weeks resulted in cholesterol super saturation in gallbladder bile, which promoted the formation of cholesterol gallstones (CGS). In this study, methanolic extract of J. Communis (MEJC) were examined for their antilithogenic potential along with lithogenic (LG) diet for seven weeks. Results J. Communis extracts were reduced the CGS incidence by 15–39%, the effect being a maximum in the methanol extract group. The extract markedly reduced biliary cholesterol. The cholesterol: phospholipids ratio which was 1.56 in the LG diet group was reduced to 0.92–0.97 in the J. Communis extracts groups. Serum and liver cholesterol was decreased by feeding J. Communis extracts to the LG diet. Conclusion J. Communis extracts exerted antilithogenic influence by decreasing the cholesterol hyper-secretion into bile and increasing the bile acid output thus decreasing the formation of lithogenic bile in experimental mice.

The Natural products journal, Apr 12, 2018

Current research in neuroscience, Dec 15, 2015

PLOS Neglected Tropical Diseases, Dec 11, 2015

Schistosomiasis, caused by parasitic flatworms of the genus Schistosoma, is a neglected tropical ... more Schistosomiasis, caused by parasitic flatworms of the genus Schistosoma, is a neglected tropical disease affecting hundreds of millions globally. Praziquantel (PZQ), the only drug currently available for treatment and control, is largely ineffective against juvenile worms, and reports of PZQ resistance lend added urgency to the need for development of new therapeutics. Ion channels, which underlie electrical excitability in cells, are validated targets for many current anthelmintics. Transient receptor potential (TRP) channels are a large family of non-selective cation channels. TRP channels play key roles in sensory transduction and other critical functions, yet the properties of these channels have remained essentially unexplored in parasitic helminths. TRP channels fall into several (7-8) subfamilies, including TRPA and TRPV. Though schistosomes contain genes predicted to encode representatives of most of the TRP channel subfamilies, they do not appear to have genes for any TRPV channels. Nonetheless, we find that the TRPV1-selective activators capsaicin and resiniferatoxin (RTX) induce dramatic hyperactivity in adult worms; capsaicin also increases motility in schistosomula. SB 366719, a highly-selective TRPV1 antagonist, blocks the capsaicin-induced hyperactivity in adults. Mammalian TRPA1 is not activated by capsaicin, yet knockdown of the single predicted TRPA1-like gene (SmTRPA) in S. mansoni effectively abolishes capsaicin-induced responses in adult worms, suggesting that SmTRPA is required for capsaicin sensitivity in these parasites. Based on these results, we hypothesize that some schistosome TRP channels have novel pharmacological sensitivities that can be targeted to disrupt normal parasite neuromuscular function. These results also have implications for understanding the phylogeny of metazoan TRP channels and may help identify novel targets for new or repurposed therapeutics.

Chinese Journal of Biology (Online), Mar 15, 2015

We evaluated anti-Parkinson's activity of methanolic extract of Juniperus communis (MEJC) leaves ... more We evaluated anti-Parkinson's activity of methanolic extract of Juniperus communis (MEJC) leaves in chlorpromazine (CPZ) induced experimental animal model. In this study effects of Juniperus communis (100 and 200 mg/kg, i.p.) were studied using various behavior parameters like catalepsy (bar test), muscle rigidity (rotarod test), and locomotor activity (actophotometer) and its effect on neurochemical parameters (TBARS, GSH, nitrite, and total protein) in rats. The experiment was designed, by giving chlorpromazine (3 mg/kg, i.p.) for 21 days to induce Parkinson's disease-like symptoms. Chlorpromazine significantly induced motor dysfunctions (catalepsy, muscle rigidity, and hypolocomotion) in a period of 21 days. The MEJC significantly (< 0.001) reduced catalepsy and muscle rigidity and significantly (< 0.001) increased locomotor activity in rats. The maximum reduction was observed on the 21st day at a dose of 200 mg/kg (i.p.). The MEJC extract also showed an increase in the level of reduced gutathione (GSH) (< 0.001) and total protein (< 0.001) and decreased the elevated levels of TBARS (< 0.001) and nitrite (< 0.001) preferably at a higher dose (200 mg/kg) as compared to chlorpromazine group. Thus the present study showed the neuroprotective effect of MEJC against CPZ induced Parkinson's disease-like symptoms or anti-Parkinson's activity.