Amin A Seleem | Sohag University (original) (raw)
Papers by Amin A Seleem
The Journal of Basic and Applied Zoology, 2018
Background: Acrylamide is reported for its toxicity on the central and the peripheral nervous sys... more Background: Acrylamide is reported for its toxicity on the central and the peripheral nervous system and causes paralysis. Bee venom (BV) and bradykinin-potentiating factor (BPF) have been documented for their potential therapeutic effects as anti-neuroinflammation. The study aimed to ameliorate the neurotoxic effects of acrylamide or chips by using BV or its extracted BPF. Mice were divided into 15 subgroups: control (G1.1, G1.2, G1.3) at 30, 45, and 60 days, respectively; acrylamide-(10 mg/kg b.w.; orally daily) administered subgroup for 30 days (G2.1), 45 days (G2.2), and 60 days (G2.3); chips feeding group (1/3 of daily diet) for 30 days (G3.1), 45 days (G3.2), and 60 days (G3.3); bee venom-treated group for 60 days (1.319 mg/kg b.w.) (G4.1); BPF-treated group for 60 days (2.314 mg/kg b.w.) (G4.2), day after the other day; and acrylamide-or chips-administered groups combined either with BV (G5.1, G6.1) or BPF treatment (G5.2, G6.2) for 60 days. Results: The results indicated that the approximate LD50 for BV and BPF equal to 13.19 mg/kg and 23.14 mg/kg, respectively, and the extracted BPF contains 15 amino acids. Also, the results showed abnormal gait in mice of acrylamide-administered groups which was accompanied by histopathological changes in the hippocampus, cerebellum, and cerebral cortex. A marked gradual increase of alpha-synuclein expression was noted at the studied region in the acrylamide-and chips-treated groups at 60 days of treatment as compared to control. Both BV-and BPF-treated groups either separately or in co-administration with acrylamide or with chips did not show any histopathological changes in the studied regions with downregulated expression of alpha-synuclein. Conclusion: The study concluded the neuroprotective effect of BV and its extracted BPF against neurotoxicity induced by acrylamide or chips administration.
Some Cd (II), Mn (II) and Ag (I) complexes derived from Schiff base ligand, obtained by the conde... more Some Cd (II), Mn (II) and Ag (I) complexes derived from Schiff base ligand, obtained by the condensation of 4-Nitrobenzaldehyde and 2-amino-3-hydroxypyridine were synthesized. The complexes were characterized by elemental analysis, molar conductivity, magnetic susceptibility, IR, UV-Vis spectral data and thermal analysis. The complexes were found to be non-electrolytic in nature depending on value of molar conductance. From the spectral data, an octahedral geometry has been approached for all the complexes except Ag (I) complex which is tetrahedral. Moreover, the metal complexes have been tested for their antibacterial and antifungal activity. Furthermore, DNA interaction of these complexes was tempted by using Electronic spectra, viscosity measurements and gel electrophoresis. The experimental results indicated that the investigated complexes could associate with DNA via intercalative mode and showed a different DNA binding activity.
The Journal of Basic & Applied Zoology, 2016
International Journal of Nanomaterials and Chemistry, 2016
Some Cd (II), Mn (II) and Ag (I) complexes derived from Schiff base ligand, obtained by the conde... more Some Cd (II), Mn (II) and Ag (I) complexes derived from Schiff base ligand, obtained by the condensation of 4-Nitrobenzaldehyde and 2-amino-3-hydroxypyridine were synthesized. The complexes were characterized by elemental analysis, molar conductivity, magnetic susceptibility, IR, UV-Vis spectral data and thermal analysis. The complexes were found to be non-electrolytic in nature depending on value of molar conductance. From the spectral data, an octahedral geometry has been approached for all the complexes except Ag (I) complex which is tetrahedral. Moreover, the metal complexes have been tested for their antibacterial and antifungal activity. Furthermore, DNA interaction of these complexes was tempted by using Electronic spectra, viscosity measurements and gel electrophoresis. The experimental results indicated that the investigated complexes could associate with DNA via intercalative mode and showed a different DNA binding activity.
Complexes of Fe (II) and Cu (II) with a tridentate Schiff base, 2-((z)-(pyridine-2-ylimino) methy... more Complexes of Fe (II) and Cu (II) with a tridentate Schiff base, 2-((z)-(pyridine-2-ylimino) methyl) naphthalene-1-ol derived from 2-hydroxy-1-napthaldehyde and 2-aminopyridine were synthesized. Both the ligand and its complexes were characterized on the basis of microanalysis, melting point, 1 H and 13 CNMR, molar conductivity, thermal analysis, IR and UV/Vis spectral studies. From analytical data, the stoichiometry of the complexes was found to be 1: 2 (metal: ligand). The magnetic susceptibilities of all complexes at room temperature were consistent with octahedral geometry. The molar conductance values suggest nonelectrolytes. The IR spectra revealed that the metal ions coordinated through azomethine nitrogen, pyridine nitrogen of and phenolic oxygen of the ligand. The particle size of iron and copper complexes has been investigated by TEM. Antibacterial and antifungal activities in vitro were performed against three types of G+ and G-bacteria, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and three types of fungi, Aspergillus flavus, Trichophyton rubrum and Candida albicans with determination of minimum inhibitory concentrations of ligand and metal complexes. Both complexes showed highly effective antibacterial activities against the tested bacteria and fungi; therefore, these complexes can be used as antibiotic. Furthermore, DNA interaction of Schiff base complexes was monitored by electronic spectra, hydrodynamic measurements and gel electrophoresis. It was found that the prepared complexes could bind to DNA in an intercalating mode.
The Journal of Basic & Applied Zoology, 2015
Lead is a poison that affects virtually every system in the body. The current study was planned t... more Lead is a poison that affects virtually every system in the body. The current study was planned to examine the toxic effects of lead acetate on the histological picture of testes, and the protective roles of DMSA, combined vitamins C and E, and DMSA combined with vitamin C plus vitamin E against the histopathological changes in the testes of albino rats induced by lead acetate. Oral administration of lead acetate caused necrosis of spermatogenic cells in the seminiferous tubules, congestion of interstitial blood vessels, severe interstitial edema and complete necrosis in the seminiferous tubules. Co-administration of DMSA with lead acetate minimized the histopathological changes exhibited by lead acetate in the affected organ compared with lead acetateintoxicated rats. Lead acetate combined with vitamin C plus vitamin E supplemented rats showed mild congestion of the interstitial blood vessels and the seminiferous tubules with its components appeared normal compared to DMSA treated rats. Treatment with DMSA combined with vitamin C plus vitamin E showed more or less normal histological appearance of the testes in lead acetate induced histopathological changes in the affected organ. These results show that DMSA, as a chelating agent for lead, and the combination of vitamins C and E as antioxidants reduced the toxic effects of lead on the histological structure of testes in albino rats but did not provide complete protection. Whereas, the supplementation of DMSA combined with both vitamins C and E provide complete protection against toxicity induced by lead in the testes of albino rats.
The Journal of Basic and Applied Zoology, 2018
Background: Acrylamide is reported for its toxicity on the central and the peripheral nervous sys... more Background: Acrylamide is reported for its toxicity on the central and the peripheral nervous system and causes paralysis. Bee venom (BV) and bradykinin-potentiating factor (BPF) have been documented for their potential therapeutic effects as anti-neuroinflammation. The study aimed to ameliorate the neurotoxic effects of acrylamide or chips by using BV or its extracted BPF. Mice were divided into 15 subgroups: control (G1.1, G1.2, G1.3) at 30, 45, and 60 days, respectively; acrylamide-(10 mg/kg b.w.; orally daily) administered subgroup for 30 days (G2.1), 45 days (G2.2), and 60 days (G2.3); chips feeding group (1/3 of daily diet) for 30 days (G3.1), 45 days (G3.2), and 60 days (G3.3); bee venom-treated group for 60 days (1.319 mg/kg b.w.) (G4.1); BPF-treated group for 60 days (2.314 mg/kg b.w.) (G4.2), day after the other day; and acrylamide-or chips-administered groups combined either with BV (G5.1, G6.1) or BPF treatment (G5.2, G6.2) for 60 days. Results: The results indicated that the approximate LD50 for BV and BPF equal to 13.19 mg/kg and 23.14 mg/kg, respectively, and the extracted BPF contains 15 amino acids. Also, the results showed abnormal gait in mice of acrylamide-administered groups which was accompanied by histopathological changes in the hippocampus, cerebellum, and cerebral cortex. A marked gradual increase of alpha-synuclein expression was noted at the studied region in the acrylamide-and chips-treated groups at 60 days of treatment as compared to control. Both BV-and BPF-treated groups either separately or in co-administration with acrylamide or with chips did not show any histopathological changes in the studied regions with downregulated expression of alpha-synuclein. Conclusion: The study concluded the neuroprotective effect of BV and its extracted BPF against neurotoxicity induced by acrylamide or chips administration.
Some Cd (II), Mn (II) and Ag (I) complexes derived from Schiff base ligand, obtained by the conde... more Some Cd (II), Mn (II) and Ag (I) complexes derived from Schiff base ligand, obtained by the condensation of 4-Nitrobenzaldehyde and 2-amino-3-hydroxypyridine were synthesized. The complexes were characterized by elemental analysis, molar conductivity, magnetic susceptibility, IR, UV-Vis spectral data and thermal analysis. The complexes were found to be non-electrolytic in nature depending on value of molar conductance. From the spectral data, an octahedral geometry has been approached for all the complexes except Ag (I) complex which is tetrahedral. Moreover, the metal complexes have been tested for their antibacterial and antifungal activity. Furthermore, DNA interaction of these complexes was tempted by using Electronic spectra, viscosity measurements and gel electrophoresis. The experimental results indicated that the investigated complexes could associate with DNA via intercalative mode and showed a different DNA binding activity.
The Journal of Basic & Applied Zoology, 2016
International Journal of Nanomaterials and Chemistry, 2016
Some Cd (II), Mn (II) and Ag (I) complexes derived from Schiff base ligand, obtained by the conde... more Some Cd (II), Mn (II) and Ag (I) complexes derived from Schiff base ligand, obtained by the condensation of 4-Nitrobenzaldehyde and 2-amino-3-hydroxypyridine were synthesized. The complexes were characterized by elemental analysis, molar conductivity, magnetic susceptibility, IR, UV-Vis spectral data and thermal analysis. The complexes were found to be non-electrolytic in nature depending on value of molar conductance. From the spectral data, an octahedral geometry has been approached for all the complexes except Ag (I) complex which is tetrahedral. Moreover, the metal complexes have been tested for their antibacterial and antifungal activity. Furthermore, DNA interaction of these complexes was tempted by using Electronic spectra, viscosity measurements and gel electrophoresis. The experimental results indicated that the investigated complexes could associate with DNA via intercalative mode and showed a different DNA binding activity.
Complexes of Fe (II) and Cu (II) with a tridentate Schiff base, 2-((z)-(pyridine-2-ylimino) methy... more Complexes of Fe (II) and Cu (II) with a tridentate Schiff base, 2-((z)-(pyridine-2-ylimino) methyl) naphthalene-1-ol derived from 2-hydroxy-1-napthaldehyde and 2-aminopyridine were synthesized. Both the ligand and its complexes were characterized on the basis of microanalysis, melting point, 1 H and 13 CNMR, molar conductivity, thermal analysis, IR and UV/Vis spectral studies. From analytical data, the stoichiometry of the complexes was found to be 1: 2 (metal: ligand). The magnetic susceptibilities of all complexes at room temperature were consistent with octahedral geometry. The molar conductance values suggest nonelectrolytes. The IR spectra revealed that the metal ions coordinated through azomethine nitrogen, pyridine nitrogen of and phenolic oxygen of the ligand. The particle size of iron and copper complexes has been investigated by TEM. Antibacterial and antifungal activities in vitro were performed against three types of G+ and G-bacteria, Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus and three types of fungi, Aspergillus flavus, Trichophyton rubrum and Candida albicans with determination of minimum inhibitory concentrations of ligand and metal complexes. Both complexes showed highly effective antibacterial activities against the tested bacteria and fungi; therefore, these complexes can be used as antibiotic. Furthermore, DNA interaction of Schiff base complexes was monitored by electronic spectra, hydrodynamic measurements and gel electrophoresis. It was found that the prepared complexes could bind to DNA in an intercalating mode.
The Journal of Basic & Applied Zoology, 2015
Lead is a poison that affects virtually every system in the body. The current study was planned t... more Lead is a poison that affects virtually every system in the body. The current study was planned to examine the toxic effects of lead acetate on the histological picture of testes, and the protective roles of DMSA, combined vitamins C and E, and DMSA combined with vitamin C plus vitamin E against the histopathological changes in the testes of albino rats induced by lead acetate. Oral administration of lead acetate caused necrosis of spermatogenic cells in the seminiferous tubules, congestion of interstitial blood vessels, severe interstitial edema and complete necrosis in the seminiferous tubules. Co-administration of DMSA with lead acetate minimized the histopathological changes exhibited by lead acetate in the affected organ compared with lead acetateintoxicated rats. Lead acetate combined with vitamin C plus vitamin E supplemented rats showed mild congestion of the interstitial blood vessels and the seminiferous tubules with its components appeared normal compared to DMSA treated rats. Treatment with DMSA combined with vitamin C plus vitamin E showed more or less normal histological appearance of the testes in lead acetate induced histopathological changes in the affected organ. These results show that DMSA, as a chelating agent for lead, and the combination of vitamins C and E as antioxidants reduced the toxic effects of lead on the histological structure of testes in albino rats but did not provide complete protection. Whereas, the supplementation of DMSA combined with both vitamins C and E provide complete protection against toxicity induced by lead in the testes of albino rats.