Yevheniia Husak | Sumy State University (original) (raw)

Papers by Yevheniia Husak

Materials, Sep 30, 2020

Despite the high biocompatibility and clinical effectiveness of Ti-based implants, surface functi... more Despite the high biocompatibility and clinical effectiveness of Ti-based implants, surface functionalization (with complex osteointegrative/antibacterial strategies) is still required. To enhance the dental implant surface and to provide additional osteoinductive and antibacterial properties, plasma electrolytic oxidation of a pure Ti was performed using a nitrilotriacetic acid (NTA)-based Ag nanoparticles (AgNP)-loaded calcium-phosphate solution. Chemical and structural properties of the surface-modified titanium were assessed using scanning electron microscopy (SEM) with energy dispersive X-ray (EDX) and contact angle measurement. A bacterial adhesion test and cell culture biocompatibility with collagen production were performed to evaluate biological effectiveness of the Ti after the plasma electrolytic process. The NTA-based calcium-phosphate solution with Ag nanoparticles (AgNPs) can provide formation of a thick, porous plasma electrolytic oxidation (PEO) layer enriched in silver oxide. Voltage elevation leads to increased porosity and a hydrophilic nature of the newly formed ceramic coating. The silver-enriched PEO layer exhibits an effective antibacterial effect with high biocompatibility and increased collagen production that could be an effective complex strategy for dental and orthopedic implant development.

Biomedicines, May 21, 2021

This article is an open access article distributed under the terms and conditions of the Creative... more This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY

Materials

Biodegradable Magnesium (Mg) implants are promising alternatives to permanent metallic prosthesis... more Biodegradable Magnesium (Mg) implants are promising alternatives to permanent metallic prosthesis. To improve the biocompatibility and with the aim of degradation control, we provided Plasma Electrolytic Oxidation (PEO) of pure Mg implant in silicate-based solution with NaOH (S1 250 V) and Ca(OH)2 (S2 300 V). Despite the well-structured surface, S1 250 V implants induced enormous innate immunity reaction with the prevalence of neutrophils (MPO+) and M1-macrophages (CD68+), causing secondary alteration and massive necrosis in the peri-implant area in a week. This reaction was also accompanied by systemic changes in visceral organs affecting animals’ survival after seven days of the experiment. In contrast, S2 300 V implantation was associated with focal lymphohistiocytic infiltration and granulation tissue formation, defining a more favorable outcome. This reaction was associated with the prevalence of M2-macrophages (CD163+) and high density of αSMA+ myofibroblasts, implying a resol...

ACS Applied Materials & Interfaces

Nanomaterials

Maxillary sinus augmentation is a commonly used procedure for the placement of dental implants. H... more Maxillary sinus augmentation is a commonly used procedure for the placement of dental implants. However, the use of natural and synthetic materials in this procedure has resulted in postoperative complications ranging from 12% to 38%. To address this issue, we developed a novel calcium deficient HA/β-TCP bone grafting nanomaterial using a two-step synthesis method with appropriate structural and chemical parameters for sinus lifting applications. We demonstrated that our nanomaterial exhibits high biocompatibility, enhances cell proliferation, and stimulates collagen expression. Furthermore, the degradation of β-TCP in our nanomaterial promotes blood clot formation, which supports cell aggregation and new bone growth. In a clinical trial involving eight cases, we observed the formation of compact bone tissue 8 months after the operation, allowing for the successful installation of dental implants without any early postoperative complications. Our results suggest that our novel bone ...

2022 IEEE 12th International Conference Nanomaterials: Applications & Properties (NAP)

Progress on Chemistry and Application of Chitin and its Derivatives

Chitosan electrospun nanofibres were synthesised in two different trifluoroacetic acid (TFA)/dich... more Chitosan electrospun nanofibres were synthesised in two different trifluoroacetic acid (TFA)/dichloromethane (DCM) solvent ratios and then neutralised in aqueous and ethanol sodium-based solutions (NaOH and Na2CO3) to produce insoluble materials with enhanced biological properties for regenerative and tissue engineering applications. Structural, electronic, and optical properties and the swelling capacity of the prepared nanofibre membrane were studied by scanning electron microscopy, Fourier-transform infrared spectroscopy, and photoluminescence. Cell viability (with the U2OS cell line) and antibacterial properties (against Staphylococcus aureus and Escherichia coli) assays were used to assess the biomedical potential of the neutralised chitosan nanofibrous membranes. A 7:3 TFA/DCM ratio allows for an elaborate nanofibrous membrane with a more uniform fibre size distribution. Neutralisation in aqueous NaOH only maintains a partial fibrous structure. At the same time, neutralisation...

В основу гіпотези наукової розробки покладені відомості про наявність гемостатичних властивостей ... more В основу гіпотези наукової розробки покладені відомості про наявність гемостатичних властивостей деяких похідних хітозану. Для перевірки гіпотези нами створена лінійка гелей з хітозану різної молекулярної маси з використанням різних розчинників (ацетат, аскорбінова, молочна кислоти). З гелей шляхом ліофільного висушування формувались губки, гемостатичні властивості яких перевірялись методом blood-clotting тесту. В результаті виконання етапу були розроблені моделі кровотечі з судин великого діаметру та паренхіматозних органів, які дозволять проводити відтворюваний експеримент з дослідження гемостатичної активності засобів медичного призначення. Доведено, що сорбція рідкої частини крові не має залежності від виду матеріалу з хітозану, в той час як молекулярна маса хітозану суттєво впливає на процеси тромбоутворення. Матеріали з молекулярною масою 200 кДа викликають адгезію тромбоцитів на поверхні зразків та активують їх, що виявляється у зміні форми елементів крові. Доведено, що механ...

ACS Applied Materials & Interfaces

Molecules

Chitosan, a natural biopolymer, is an ideal candidate to prepare biomaterials capable of preventi... more Chitosan, a natural biopolymer, is an ideal candidate to prepare biomaterials capable of preventing microbial infections due to its antibacterial properties. Electrospinning is a versatile method ideally suited to process biopolymers with minimal impact on their physicochemical properties. However, fabrication parameters and post-processing routine can affect biological activity and, therefore, must be well adjusted. In this study, nanofibrous membranes were prepared using trifluoroacetic acid and dichloromethane and evaluated for physiochemical and antimicrobial properties. The use of such biomaterials as potential antibacterial agents was extensively studied in vitro using Staphylococcus aureus and Escherichia coli as test organisms. The antibacterial assay showed inhibition of bacterial growth and eradication of the planktonic cells of both E. coli and S. aureus in the liquid medium for up to 6 hrs. The quantitative assay showed a significant reduction in bacteria cell viability ...

2018 IEEE 8th International Conference Nanomaterials: Application & Properties (NAP)

Magnesium alloys attract great attention as prospective bone implants due to their biocompatibili... more Magnesium alloys attract great attention as prospective bone implants due to their biocompatibilities, physical properties and an ability to degrade completely under physiological conditions, what eliminates the need for surgical reintervention. The main problem of developing advanced Mg alloys for medical application is matching degradation with tissue healing rate. Orthopaedic metallic implants should maintain its mechanical property for at least 3 month to avoid the second fracture occurrence resulting from their fast degradation. The purpose of this research was assessment of in-vitro corrosion and surface morphology after short term in-vivo implantation of Mg based implant covered by HA.Mg alloys with the addition of Zr(0,65%), Al(1,85%) and Nd(1,25%) were used. Ca-P-based coatings were used to improve the corrosion resistance of magnesium and its alloys as well as their surface bioactivity. Hydroxyapatite (HA) coatings were obtained on Mg alloy substrates by dipping method. Simulated body fluid (SBF; pH 7,4) with ion concentrations approximately equal to those of human blood plasma resembling physiological conditions and citrate buffer with pH 5 - simulating inflammation were selected as modeling environments for in-vitro degradation test. The rod samples were implanted into the tibia bone of rats and after 1 and 5 days of implantation were taken out to observe cells adhesion on surface samples. SEM was used to assess surface morphology after in-vitro and in-vivo tests. We determined different mechanisms of HA layer corrosion - SBF solution causes the partial dissolution, while citrate solution caused complete disappearance of the coating. HA coated layer coused lower degradaion without significant pH change during the static immersion test in SBF and citrate buffer. The HA coating favored cell adhesion and rapid fibrous tissue formation.

Eastern Ukrainian Medical Journal, 2021

Introduction. Overuse and misuse of antibiotics in humans, animals, and agriculture has led to th... more Introduction. Overuse and misuse of antibiotics in humans, animals, and agriculture has led to the widespread rise of antibiotic resistance and strengthened nosocomial pathogenes' impact. Klebsiella pneumoniae became an increasing threat to public health. Nanomaterials are promising alternatives to conventional antibiotics in the fight against multi-resistant germs. Silver nanoparticles are well-known metallic nanoparticles with antimicrobial activity. Our research aimed to evaluate the spreading of K. pneumonia resistant to antibiotics at hospital and assess the effectiveness of Ag NPs against multi-resistant clinical strains of K. pneumoniae. Material and methods. K. pneumoniae strains were isolated and identified with the use of conventional bacteriological techniques. Susceptibility of the microorganisms was assessed to inhibitors of β-lactamases, carbapenems, macrolides, oxazolidinones, and other groups of antibiotics with use Kirby-Bauer disk diffusion method. The capabili...

2020 IEEE 10th International Conference Nanomaterials: Applications & Properties (NAP), 2020

Pure magnesium (Mg) degrades very quickly and uncontrollably upon contact with water. It also oxi... more Pure magnesium (Mg) degrades very quickly and uncontrollably upon contact with water. It also oxidizes in the air. Plasma electrolytic oxidation (PEO) is a promising method for surface modification of metal alloys, which can contribute to controlling corrosion of Mg. Additionally, modifications with the PEO lead to the formation of mesoporous topography and changes in physical and chemical properties of Mg surfaces, e.g., their wettability, and to increase in their surface area. Our project aimed at modification of surfaces of Mg implant by the PEO method using silicate-based bath electrolyte in order to enhance resistance to corrosion, biocompatibility, and antibacterial properties of the implants. The use of silicate in the PEO bath electrolyte leads to changes in the surface morphology. The obtained surface layers acquire rich morphology with pores and craters of various sizes. This substantially increases the total surface area and change wettability. In addition, the formation of silicate oxide layers provides protection from corrosion and can lead to an improvement in the long-term stability of implants.

2020 IEEE 10th International Conference Nanomaterials: Applications & Properties (NAP), 2020

Plasma electrolytic oxidation (PEO) is a promising method for the development of a new generation... more Plasma electrolytic oxidation (PEO) is a promising method for the development of a new generation of oxide coatings for degradable medical devices made from Mg and its alloys. PEO coatings could protect Mg-based products from fast uncontrolled corrosion and improve biocompatibility. It is still under development the ideal solution that provides a uniform biocompatible and corrosion-resistant layer. Current research investigates the morphology characteristics of silicate and phosphate-based ceramic coatings made by PEO in different modes. PEO in a silicate-based solution leads to the formation of a ceramic layer with a crater-like morphology with uniformly distributed pores and demonstrates high wettability. At the same time, the PEO coating is formed in a phosphate-based solution that characterizes bigger pore size and lower pore distribution. Oxide layer films prevent initial S. aureus adhesion on the surfaces of the specimens. This makes it possible to consider the formed PEO coatings for degradable medical implants.

2017 IEEE 7th International Conference Nanomaterials: Application & Properties (NAP), 2017

In recent years new class of biodegradable metal emerges as an alternative for biomedical implant... more In recent years new class of biodegradable metal emerges as an alternative for biomedical implants. There are three metals (Iron, Zink and Magnesium) considered as the basis of biodegradable materials for medical application. Degradation of Mg under the physiological conditions avoids reoperation to remove bone implant, but still they didn't become the optimal material controlling biodegradation and tailoring alloy composition and microstructure depending on texture, grain size, manufacturing method and post-processing techniques. The purpose of this research was assessment of physical and chemical characteristics of Mg alloys in different environments that simulated natural. Mg alloys with the addition of Zr(0,65%), Al(1,85%) and Nd(1,25%) were used in experiment. Static and dynamic degradation in Simulated Body Fluid and citrate buffer were used to determine alloy corrosion properties. The pH value of the solution was recorded after immersion tests every 3, 6, 18 and 24 hours....

2021 IEEE 11th International Conference Nanomaterials: Applications & Properties (NAP), 2021

Degradable implants occupy the significant position in orthopedic industry. Magnesium is an appro... more Degradable implants occupy the significant position in orthopedic industry. Magnesium is an appropriate degradable material due to its good biocompatibility and degradability properties in addition to advantageous mechanical properties. Improving the material quality, especially corrosion resistance and surface active, could be occur through the chemicals and parameters of the Plasma Electrolytic Oxidation process. Voltage reduction could reduce cost involved and make more environmentally friendly. Doubling the concentration of the sodium phosphate worked at low voltage. The surface morphology showed well-developed structure. Obtained coatings were without cracks and with good thickness size. Increasing wettability point to enhancing bioactive properties.

Materials, Sep 30, 2020

Biomedicines, May 21, 2021

Materials

ACS Applied Materials & Interfaces

Nanomaterials

2022 IEEE 12th International Conference Nanomaterials: Applications & Properties (NAP)

Progress on Chemistry and Application of Chitin and its Derivatives

ACS Applied Materials & Interfaces

Molecules

2018 IEEE 8th International Conference Nanomaterials: Application & Properties (NAP)

Eastern Ukrainian Medical Journal, 2021

2020 IEEE 10th International Conference Nanomaterials: Applications & Properties (NAP), 2020

2017 IEEE 7th International Conference Nanomaterials: Application & Properties (NAP), 2017

2021 IEEE 11th International Conference Nanomaterials: Applications & Properties (NAP), 2021