Arash Khaki | Tabriz University Of Medical Sciences (original) (raw)
Papers by Arash Khaki
Maturitas, 2009
Objectives: Testosterone deficiency is having an increasing impact on men's health because of glo... more Objectives: Testosterone deficiency is having an increasing impact on men's health because of global aging, increasing obesity, diabetes and environmental factors and an explanation is needed why so few androgen deficient men are being treated. Methods: Demographic data for men over the age of 50 from different regions of the world have been compared with the number of men in that age group estimated from sales figures to be receiving testosterone treatment. Results: Based on the 20% of men over 50 in the general population who are expected to have testosterone deficiency symptoms, on average only 0.69% these men in most European countries were receiving treatment. The proportion was higher in the UK (1.00%) and Germany (1.89%), but lower in France (0.49%), Italy (0.51%) and Russia (0.54%). Australia had higher figures (1.64%), in spite of tight state control measures on androgen use. The USA has the highest treatment rate (7.96%) and this is increasing rapidly. Based on symptoms plus low total and free testosterone levels, androgen deficiency would be diagnosed in at least 5% of men over 50, and percentage treatment rates therefore four times higher. However, even on that basis, only in the USA do these exceed 10%. Conclusions: International action is urgently needed to raise awareness in the medical profession in the various countries of these unacceptably low levels of testosterone treatment. Improvement in this requires education and motivation of doctors and those regulating the healthcare systems.
Polycystic ovary syndrome (PCOS) is the most frequent cause of female infertility, affecting abou... more Polycystic ovary syndrome (PCOS) is the most frequent cause of female infertility, affecting about 5 - 10% of women in age of p rocreation. See antioxidants effects of Allium cepa seeds ethanol extract on experimental PCO induced by estradiol - valerat ( PPA) in rats. Wistar female rat (n=60) were allocated into three groups, control (n=30), C1: an equal volume of (0.9% NaCl); C2: ext ract ( 0.3 cc/rat/orally/daily); C3: Sesame oil(0 . 3cc/rat/orally/daily) and test groups (n=30), that subdivided into groups of 3 , one group received extract supplement (0. 3 cc Sesame oil+ 0. 3 cc Allium Cepa /rat/orally/daily), second and third groups were indu ced PCO by single injection of estradiol - valerate (4mg/rat/IM),third group , was received extract supplement, for 60 consequence day s . Animals were kept in standard conditions. In last day of study the blood samples of r ats in whole groups were removed and pr epared to biochemical analysis. level of TAC, Superoxide dismutase and catalas...
PCOS produces symptoms in approximately 5% to 10% of women of reproductive age (12–45 years old).... more PCOS produces symptoms in approximately 5% to 10% of women of reproductive age (12–45 years old). It is thought to be one of the leading causes of female subfertility. To confirm the role of nutrition with omega-3 polyunsaturated fatty acid on controlled of experimental PCO induced by estradiol-valerat (PPA) in rats, this study was done. Wistar female rat (n=50) were allocated into control (n=10) and test groups (n=40), test group subdivided into groups of 4, G1, received omega-3 (60 mg/rat/orally/daily),G2 induced PCO by single injection of estradiolvalerate (4mg/rat/IM), G3 and G4 groups were induced PCO by single injection of estradiol-valerate (4mg/rat/IM),G3 & G4 groups was received omega-3 (60 mg/rat) ,and G4, received lower carbohydrate feeding ,for 60 consequence day. In sixty day 5cc blood samples and ovarian tissues of Rats in whole groups were removed and prepared to biochemical pathological analysis. hemorrhage, hyperemia and fibrosis were seen in pco groups, these side ...
The present study examined the efficacy of Ocimum basilicum extract, a natural herb, with antioxi... more The present study examined the efficacy of Ocimum basilicum extract, a natural herb, with antioxidant properties, against testicular toxicity induced by Lead, which is one of the most important toxic heavy metals. The intoxicated rats showed decreased cell proliferation was reflected by a decrease in Ki-67 expression, whereas the increase in apoptotic rate was associated with a decrease in the Bcl/Bax ratio. Concomitant treatment with aqueous basil extract led to an improvement in histological, morphometrical and immunohistochemical changes induced by lead. The beneficial effects of basil extract could be attributed to its antioxidant properties.
Crescent Journal of Medical and Biological Sciences, 2016
Objective: Infectious infertility is considered by the World Health Organization (WHO) as a main ... more Objective: Infectious infertility is considered by the World Health Organization (WHO) as a main problem in sexual life and public health. The aim of the present study was to investigate the antioxidant properties and the effect of Allium cepa (onion) juice on the tissue of testis and seminiferous tubules affected by Escherichia coli. Materials and Methods: Thirty-Two adult Wistar male rats aging 2.5 to 3 months divided to four groups of 8 rats. Enterotoxigenic E. coli (serotype 0114) used to infect the rats. Onions prepared from the district Ilkhichi, Iran which were used for two groups. Following the infection, pathologic samples were prepared from the tissue of the sperms which were investigated through hematoxylin & eosin (H & E) staining. In addition, the motility, vitality, the number of sperms, total antioxidant capacity (TAC), luteinizing hormone (LH), and testosterone were evaluated as well. Results: Results indicated that in the control group all the seminiferous tubules a...
INDONESIAN JOURNAL OF PHARMACY, 2013
Citrullus vulgaris is an antioxidant and has been shown to reduce oxidative stress. Previous stud... more Citrullus vulgaris is an antioxidant and has been shown to reduce oxidative stress. Previous studies confirmed that antioxidants have essential effect on infertility through participating in reactive oxygen’s species. Chronic hyperglycemia is known to cause infertility in diabetes disease. Wistar male rats (n=40) were allocated into three groups: control group(n=10), Citrullus vulgaris seeds extract (CVE) group that received 55mg/kg by gavage method (n=10), and Diabetic group that received 55mg/kg (IP) streptozotocin (STZ) (n=20). The last group was subdivided into two groups of 10. STZ group and treatment group. Treatment group received 55mg/kg (IP) STZ plus 55mg/kg CVE, daily for 4weeks; however, the control group just received an equal volume of (0.9% NaCl) daily (gavage). Diabetes was induced by a single (IP) injection of streptozotocin (55mg/kg). Animals were kept in standard condition. In 28th day, 5cc bloodn sample was taken from every rat for biochemical analysis. Collecting...
life science journal, 2013
Polycystic ovary syndrome (PCOS) is the most frequent cause of female infertility, affecting abou... more Polycystic ovary syndrome (PCOS) is the most frequent cause of female infertility, affecting about 5– 10% of women in age of procreation. Apoptotic effects of Allium cepa seeds ethanol extract on experimental PCO induced by estradiol-valerat (PPA) in rats. Wistar female rat (n=60) were allocated into three groups, control (n=30); C1, an equal volume of (0.9% NaCl); C2, extract (0.3cc/rat/orally/daily); C3, Sesame oil(0.3cc/rat/orally/daily) and test groups (n=30), that subdivided into groups of 3 ,one group received extract supplement (0.3cc Sesame oil+ 0.3cc Allium Cepa /rat/orally/daily),second and third groups were induced PCO by single injection of estradiolvalerate (4mg/rat/IM),third group received extract supplement, for 60 consequence days. Animals were kept in standard conditions. In last day of study the blood samples and ovarian tissue of rats in whole groups were removed and prepared to biochemical and pathology analysis. Means of hyperaemia, number of cyst and granulosa ...
Cell Journal (Yakhteh), 2019
Objective Panax ginseng is a popular traditional herb that has been used in complementary and alt... more Objective Panax ginseng is a popular traditional herb that has been used in complementary and alternative medicine in eastern Asia, and it possesses pharmacologically active compounds like ginsenosides (GSs). This study aimed to investigate the impact of Panax ginseng extract (PGE) at different concentrations on in vitro follicular function and development in a three-dimensional (3D) culture system fabricated using sodium alginate after 12 days of culture. Materials and Methods In this experimental study, preantral follicles (n=661) were mechanically isolated from the ovaries of 14-day-old female NMRI mice using 29-gauge insulin syringes. Follicles were individually capsulated within sodium alginate, and divided into four groups including control and experimental groups 1, 2, and 3. Then, they were cultured for 12 days in the medium supplemented with different concentrations of PGE (0, 50, 100, and 500 µg/ mL, for control groups and groups 1, 2 and 3, respectively). At the end of th...
International Journal of Fertility & Sterility, 2018
Background There is some evidence indicating that Matricaria chamomile (MC) had protective effect... more Background There is some evidence indicating that Matricaria chamomile (MC) had protective effects on ischemia- reperfusion. In the present study, a rat model was used to investigate the effect of hydroalcoholic extract of MC on torsion/detorsion-induced testis tissue damage. Materials and Methods In this experimental study, 28 male Wistar rats were randomly divided into 4 groups as follows: G1, Sham operated; G2, testicular torsion/detorsion (T/D); G3, rats with testicular torsion/detorsion that received 300 mg/kg of MC extracts 30 minutes before detorsion (T/DMC); and G4, healthy rats that received 300 mg/kg of MC extracts (MC). Also, the reperfusion period was 24 hours. After blood sampling, the oxidative stress marker [e.g. superoxide dismutase (SOD) levels], blood levels of testosterone, and anti-oxidant enzyme levels [e.g. glutathione peroxidase (GPx)] were assessed by ELISA methods. Serum activity of malondialdehyde (MDA) was evaluated by spectrophotometry. Another assessment...
Archives of Medical Science, 2020
Introduction: To evaluate the effect of a herbal mixture (i.e., Mentha spicata, Zingiber officina... more Introduction: To evaluate the effect of a herbal mixture (i.e., Mentha spicata, Zingiber officinale, Cinnamomum zeylanicum, and Citrus sinensis) alone and in combination with clomiphene citrate (CC) compared to CC on the treatment of polycystic ovary syndrome (PCOS). Material and methods: This single-blind randomized clinical trial was conducted on 60 infertile participants with PCOS who were randomly divided into three groups. After spontaneous or progestin-induced withdrawal bleeding, group 1 (n = 20) received routine treatment with CC (50-150 mg) for three menstrual cycles from the 3 rd to 5 th day of menstruation for 5 days while group 2 (n = 20) and group 3 (n = 20) received herbal mixture capsules 700 mg/day and the herbal mixture along with CC for 3 months, respectively. Finally, several related parameters were measured, including the level of sex steroids, homeostatic model assessment for insulin resistance (HOMA-IR), lipid profile (primary outcomes), thyroid hormones, and clinical features. The analysis was based on intention-to-treat analysis. Results: No statistically significant differences were observed between the groups in terms of socio-demographic characteristics. However, after adjustment for baseline, luteinizing hormone (aMD = 4.9; 95% CI: 3.7-6.2), luteinizing hormone/follicle-stimulating hormone (aMD = 0.9; 95% CI: 0.7-1.2), total testosterone (aMD =-0.12; 95% CI:-0.2 to-0.01) in group 2 and free testosterone (aMD =-6.0; 95% CI:-9.7 to-2.3) in group 3 revealed a significant difference compared to group 1. In addition, HOMA-IR in group 2 (aMD =-1.3; 95% CI:-2.4 to-0.2) decreased significantly compared to group 1. Further, total cholesterol, triglycerides, low-density lipoprotein cholesterol, and very low-density lipoprotein cholesterol decreased significantly
Biomolecules, 2019
This paper aimed to evaluate the effect of herbal mixture (Mentha spicata, Zingiber officinale, C... more This paper aimed to evaluate the effect of herbal mixture (Mentha spicata, Zingiber officinale, Cinnamomum zeylanicum, and Citrus sinensis) only and along with clomiphene citrate (CC) compared to CC on serum antioxidants, glycemic status, menstrual regulation, and rate of pregnancy. This single-blind randomized clinical trial was carried out on 60 infertile participants with polycystic ovary syndrome (PCOS) willing to be pregnant. They were randomly allocated into group 1 (n = 20) who received routine dose of CC pills (50–150 mg) for three menstrual cycles from the fifth day of menstruation for five days; group 2 (n = 20) who consumed herbal mixture daily (700 mg); and group 3 (n = 20) who used up herbal mixture along with CC for 3 months. Catalase (CAT), glutathione peroxidase (GPx), superoxide dismutase (SOD), malondialdehyde (MDA), fasting blood sugar (FBS), insulin, and homeostatic model assessment for insulin resistance (HOMA-IR) were measured in their blood samples. No statist...
International Journal of Women's Health and Reproduction Sciences, 2015
Introduction Infertility is defined by the failure to achieve a clinical pregnancy after 12 month... more Introduction Infertility is defined by the failure to achieve a clinical pregnancy after 12 months or more of regular unprotected sexual intercourse which involves 10%-15% of couples. Male factor is responsible for 40% of infertility cases. The leading cause of male infertility is impaired sperm production caused by various factors such as infections, genetic disorders, medications, aging, social and environmental factors (1-5). Oxidative damage due to free radicals is one of the major causes of idiopathic oligospermia, even with undesirable impact on the structure of sperm produces abnormal sperm. For example, in diabetic patients by producing reactive oxygen species (ROS), free radicals of nitric oxide cause oxidative stress and thus result in testicular insufficiency through affecting spermatogenesis and estrogenic pathways (6-8). Varicocele which has been observed in 19% to 41% of infertile men is a common cause of male infertility (9-11). Increased levels of ROS and oxidative levels (oxidative stress) is one of the proposed mechanisms for impaired spermatogenesis. Vacuole formation in testis tissue is a sign of oxidative stress and is associated with aging which can be neutralized by antioxidants (12-15). Lack of vitamin A, elements such as flavonoids, carnitine, folate, zinc and selenium and overall reduction of antioxidants in the diet can be one of the reasons for infertility especially oligospermia and asthenozoospermia in humans (16-18). Antioxidants like vitamin E, vitamin C, glutathione, coenzyme Q10 and natural polyphenols such as flavonoids and phenolic acids by reducing the damage caused by free radicals, strengthening blood-testis barrier and protecting and repairing sperm DNA, are effective in the treatment of male infertility (19-21). Assisted reproductive techniques (ART) such as in vitro fertilization (IVF), zygote intra-fallopian transfer (ZIFT) and intra-cytoplasmic sperm insemination (ICSI) has solved many problems of infertility; however, due to high cost, they are not affordable by many of infertile couples. On the other hand, there are effective medications which are less expensive and available for a wide range of couples. The bioactive compounds in plant extracts are highly regarded because it is derived from natural sources and compatible with vital systems. Using herbal medicine to increase fertility and improve factors such as hormonal imbalance, oligospermia, low motility of sperm, prostatitis, varicocele, etc has been considered for a long time. Previous studies on laboratory animals indicated that plants such as Allium cepa, Zingiber officinale, Ocimum basilicum, Cinnamomum verum, Citrus sinensis' peel, Citrullus lanatus and Daucus carota' seeds as a rich source of
International Journal of Women's Health and Reproduction Sciences, 2014
Objectives: Polycystic ovary syndrome (PCOS) is the most frequent cause of female infertility, af... more Objectives: Polycystic ovary syndrome (PCOS) is the most frequent cause of female infertility, affecting about 4% to 8% of women in the age of procreation. For evaluation the protective effects of omega-3 polyunsaturated fatty acid on ovarian structure in experimental PCO induced by estradiol-valerat, this research was done. Materials and Methods: Wistar female rats (n=40) were allocated into four groups, one control (n=10) and three test groups (n=30), that one group received omega-3 (60 mg/rat/orally/daily), second and third groups were induced PCO by single injection of estradiol-valerate (16mg/ kg/ i.m), third group also received omega-3 (240 mg/kg) for 60 consequence days. Animals were kept in standard conditions. On day 60, the ovarian tissue of Rats in whole groups were removed and prepared for pathological analysis. Results: vacuolated area and rough endoplasmic reticulum expanded, de-granulated, disorganized were seen in PCO groups; however, these side effects decreased in the groups that received omega-3 significantly (p<0.05) in comparison to experiment groups and ovarian weights in PCO experimental decreased significantly (p<0.05). Conclusion: Results revealed that administration of omega-3 could significantly treat PCO. This suggested that polyunsaturated fatty acid could diminish negative side effects of PCO on ovary tissue.
Journal of Obstetrics and Gynaecology, 2021
Abstract The current study was designed to investigate the effect of eugenol on histopathological... more Abstract The current study was designed to investigate the effect of eugenol on histopathological changes and oxidative stress caused by torsion/detorsion in the ovary of adult female rats. In this study, forty-eight female Wistar rats were classified into six groups; Sham and 2 healthy group receiving 30, 60 mg/kg eugenol; ovarian torsion/detorsion; and 2 experimental groups receiving 30, 60 mg/kg eugenol. After ten days, the plasma levels of oestrogen, testosterone, and some oxidative stress markers were assessed. also, the histomorphometric study was performed. A marked degenerative changes in the TD group was observed (p < .001). The oestrogen, GPX, and SOD levels were remarkably declined in the G2 group, while they were reversed to the baseline values in groups receiving eugenol. The concentration of malondialdehyde (MDA) was remarkably increased during the ischaemia (p < .001). The treatment with eugenol significantly diminished MDA levels in different groups (p < .001). Our finding indicated that eugenol could protect the ovarian tissue against oxidative stress and tissue injury induced by torsion/detorsion. IMPACT STATEMENT What is already known about this subject? Ovarian torsion is one of the commonest gynecological emergencies in all age groups of the female gender. Timely diagnosis and management of ovarian torsion are crucial, especially for women of reproductive age. Detorsion is one of the interventions used for the prevention of ovarian tissue damage. Ovarian ischaemia/reperfusion is a pathophysiological condition in which decreased blood flow, and oxygen deficiency (ischaemia) are observed in ovarian tissues as a result of ovarian torsion. Following torsion, the inflammatory response induced by detorsion (reperfusion) leads to vascular endothelial cell apoptosis and microcirculation abnormalities, which are responsible for the cause of ovarian tissue damage. What do the results of this study add? This study found that eugenol, an antioxidant and anti-inflammatory agent, could be used experimentally to diminish the I/R damage in the ovary through the attenuation of detrimental histological events, decreasing the serum level of MDA and testosterone, and increasing the level of SOD and GPX enzymes. To date, there is no report on the application of eugenol for diminishing T/D-induced oxidative stress in the ovary. What are the implications of these findings for clinical practice? Eugenol has been shown to possess therapeutic properties in patients with ovarian torsion. Further clinical studies are necessary to prove the beneficial effect of eugenol on the prevention of I/R-induced ovarian damage.
Objectives: Rupture of membranes prior to the onset of labor is known as Premature Rupture of Mem... more Objectives: Rupture of membranes prior to the onset of labor is known as Premature Rupture of Membranes ( PROM). Early and correct diagnosis is crucial in order to prevent fetal and maternal risks that can be life threatening. We aimed to investigate the diagnostic ability of the tests in PROM. Materials and Methods: Nitrazine test, fern test, amnio-dye test, biochemical tests (insulin-like growth factor binding protein-1 and placental alpha microglobulin-1) were evaluated in terms of effectiveness in diagnosis of PROM. Results: A gold standard method has not yet been defined in PROM. Diagnostic tests (nitrazine test, fern test, Insulin-like Growth Factor Binding Protein-1 IGFBP-1 and Placental Alpha Microglobulin-1 PAMG-1) should be used when the diagnosis is not certain following history, examination with sterile speculum and ultrasonography evaluation. Conclusion: IGFBP-1 and PAMG-1 are tests based on bedside immunochromatographic method. Especially, PAMG-1 comes into prominence ...
Academic Journals, Apr 30, 2009
The aim of this study was to investigate the comparative effects of aminoglycosides and fluoroqui... more The aim of this study was to investigate the comparative effects of aminoglycosides and fluoroquinolones on testis tissue in rats. Thirty male Wister rats were randomly divided into control (n=10) and experimental (n=20) groups. The experimental groups subdivided into two groups of ten. Each received 5 mg/kg (IP) Gentamicin and 72 mg/kg (IP) Ofloxacin daily for 14 days, respectively; however, the control group just received vehicle (IP). In the fourteenth day, rats were killed and testis tissues were also prepared for light and electron microscopic study.Allanimalsexposedtodrugswereseenasdepletion of germ cells, germinal cells necrosis, especially in spermatogonia and Leydig cells had an abnormal fibroblast-like appearance, abnormal space between neighbor sertoli cells, mitochondria were lost cristae and vacuolated (none energized), lyzosome seen more in cytoplasm of sertoli cells, nucleus of moiled cell was done heterochromatin. Gentamicin and Ofloxacin have negative effects on testis architecture and germinal cells damagesn rats. Therefore, it is recommended that usage of this drug have harmful side effects on male fertility.
Background and Objectives: Ciprofloxacin is a synthetic antibacterial agent belonging to Fluoroqu... more Background and Objectives: Ciprofloxacin is a synthetic antibacterial agent belonging to Fluoroquinolone drugs affecting effectively on gram-negative bacterial infectious. The aim of this study was to assess the effect of ciprofloxacin in the spermatogenesis period.
Anti-oxidative effects of citro flavonoids on spermatogenesis in rat
Maturitas, 2009
Objectives: Testosterone deficiency is having an increasing impact on men's health because of glo... more Objectives: Testosterone deficiency is having an increasing impact on men's health because of global aging, increasing obesity, diabetes and environmental factors and an explanation is needed why so few androgen deficient men are being treated. Methods: Demographic data for men over the age of 50 from different regions of the world have been compared with the number of men in that age group estimated from sales figures to be receiving testosterone treatment. Results: Based on the 20% of men over 50 in the general population who are expected to have testosterone deficiency symptoms, on average only 0.69% these men in most European countries were receiving treatment. The proportion was higher in the UK (1.00%) and Germany (1.89%), but lower in France (0.49%), Italy (0.51%) and Russia (0.54%). Australia had higher figures (1.64%), in spite of tight state control measures on androgen use. The USA has the highest treatment rate (7.96%) and this is increasing rapidly. Based on symptoms plus low total and free testosterone levels, androgen deficiency would be diagnosed in at least 5% of men over 50, and percentage treatment rates therefore four times higher. However, even on that basis, only in the USA do these exceed 10%. Conclusions: International action is urgently needed to raise awareness in the medical profession in the various countries of these unacceptably low levels of testosterone treatment. Improvement in this requires education and motivation of doctors and those regulating the healthcare systems.
Polycystic ovary syndrome (PCOS) is the most frequent cause of female infertility, affecting abou... more Polycystic ovary syndrome (PCOS) is the most frequent cause of female infertility, affecting about 5 - 10% of women in age of p rocreation. See antioxidants effects of Allium cepa seeds ethanol extract on experimental PCO induced by estradiol - valerat ( PPA) in rats. Wistar female rat (n=60) were allocated into three groups, control (n=30), C1: an equal volume of (0.9% NaCl); C2: ext ract ( 0.3 cc/rat/orally/daily); C3: Sesame oil(0 . 3cc/rat/orally/daily) and test groups (n=30), that subdivided into groups of 3 , one group received extract supplement (0. 3 cc Sesame oil+ 0. 3 cc Allium Cepa /rat/orally/daily), second and third groups were indu ced PCO by single injection of estradiol - valerate (4mg/rat/IM),third group , was received extract supplement, for 60 consequence day s . Animals were kept in standard conditions. In last day of study the blood samples of r ats in whole groups were removed and pr epared to biochemical analysis. level of TAC, Superoxide dismutase and catalas...
PCOS produces symptoms in approximately 5% to 10% of women of reproductive age (12–45 years old).... more PCOS produces symptoms in approximately 5% to 10% of women of reproductive age (12–45 years old). It is thought to be one of the leading causes of female subfertility. To confirm the role of nutrition with omega-3 polyunsaturated fatty acid on controlled of experimental PCO induced by estradiol-valerat (PPA) in rats, this study was done. Wistar female rat (n=50) were allocated into control (n=10) and test groups (n=40), test group subdivided into groups of 4, G1, received omega-3 (60 mg/rat/orally/daily),G2 induced PCO by single injection of estradiolvalerate (4mg/rat/IM), G3 and G4 groups were induced PCO by single injection of estradiol-valerate (4mg/rat/IM),G3 & G4 groups was received omega-3 (60 mg/rat) ,and G4, received lower carbohydrate feeding ,for 60 consequence day. In sixty day 5cc blood samples and ovarian tissues of Rats in whole groups were removed and prepared to biochemical pathological analysis. hemorrhage, hyperemia and fibrosis were seen in pco groups, these side ...
The present study examined the efficacy of Ocimum basilicum extract, a natural herb, with antioxi... more The present study examined the efficacy of Ocimum basilicum extract, a natural herb, with antioxidant properties, against testicular toxicity induced by Lead, which is one of the most important toxic heavy metals. The intoxicated rats showed decreased cell proliferation was reflected by a decrease in Ki-67 expression, whereas the increase in apoptotic rate was associated with a decrease in the Bcl/Bax ratio. Concomitant treatment with aqueous basil extract led to an improvement in histological, morphometrical and immunohistochemical changes induced by lead. The beneficial effects of basil extract could be attributed to its antioxidant properties.
Crescent Journal of Medical and Biological Sciences, 2016
Objective: Infectious infertility is considered by the World Health Organization (WHO) as a main ... more Objective: Infectious infertility is considered by the World Health Organization (WHO) as a main problem in sexual life and public health. The aim of the present study was to investigate the antioxidant properties and the effect of Allium cepa (onion) juice on the tissue of testis and seminiferous tubules affected by Escherichia coli. Materials and Methods: Thirty-Two adult Wistar male rats aging 2.5 to 3 months divided to four groups of 8 rats. Enterotoxigenic E. coli (serotype 0114) used to infect the rats. Onions prepared from the district Ilkhichi, Iran which were used for two groups. Following the infection, pathologic samples were prepared from the tissue of the sperms which were investigated through hematoxylin & eosin (H & E) staining. In addition, the motility, vitality, the number of sperms, total antioxidant capacity (TAC), luteinizing hormone (LH), and testosterone were evaluated as well. Results: Results indicated that in the control group all the seminiferous tubules a...
INDONESIAN JOURNAL OF PHARMACY, 2013
Citrullus vulgaris is an antioxidant and has been shown to reduce oxidative stress. Previous stud... more Citrullus vulgaris is an antioxidant and has been shown to reduce oxidative stress. Previous studies confirmed that antioxidants have essential effect on infertility through participating in reactive oxygen’s species. Chronic hyperglycemia is known to cause infertility in diabetes disease. Wistar male rats (n=40) were allocated into three groups: control group(n=10), Citrullus vulgaris seeds extract (CVE) group that received 55mg/kg by gavage method (n=10), and Diabetic group that received 55mg/kg (IP) streptozotocin (STZ) (n=20). The last group was subdivided into two groups of 10. STZ group and treatment group. Treatment group received 55mg/kg (IP) STZ plus 55mg/kg CVE, daily for 4weeks; however, the control group just received an equal volume of (0.9% NaCl) daily (gavage). Diabetes was induced by a single (IP) injection of streptozotocin (55mg/kg). Animals were kept in standard condition. In 28th day, 5cc bloodn sample was taken from every rat for biochemical analysis. Collecting...
life science journal, 2013
Polycystic ovary syndrome (PCOS) is the most frequent cause of female infertility, affecting abou... more Polycystic ovary syndrome (PCOS) is the most frequent cause of female infertility, affecting about 5– 10% of women in age of procreation. Apoptotic effects of Allium cepa seeds ethanol extract on experimental PCO induced by estradiol-valerat (PPA) in rats. Wistar female rat (n=60) were allocated into three groups, control (n=30); C1, an equal volume of (0.9% NaCl); C2, extract (0.3cc/rat/orally/daily); C3, Sesame oil(0.3cc/rat/orally/daily) and test groups (n=30), that subdivided into groups of 3 ,one group received extract supplement (0.3cc Sesame oil+ 0.3cc Allium Cepa /rat/orally/daily),second and third groups were induced PCO by single injection of estradiolvalerate (4mg/rat/IM),third group received extract supplement, for 60 consequence days. Animals were kept in standard conditions. In last day of study the blood samples and ovarian tissue of rats in whole groups were removed and prepared to biochemical and pathology analysis. Means of hyperaemia, number of cyst and granulosa ...
Cell Journal (Yakhteh), 2019
Objective Panax ginseng is a popular traditional herb that has been used in complementary and alt... more Objective Panax ginseng is a popular traditional herb that has been used in complementary and alternative medicine in eastern Asia, and it possesses pharmacologically active compounds like ginsenosides (GSs). This study aimed to investigate the impact of Panax ginseng extract (PGE) at different concentrations on in vitro follicular function and development in a three-dimensional (3D) culture system fabricated using sodium alginate after 12 days of culture. Materials and Methods In this experimental study, preantral follicles (n=661) were mechanically isolated from the ovaries of 14-day-old female NMRI mice using 29-gauge insulin syringes. Follicles were individually capsulated within sodium alginate, and divided into four groups including control and experimental groups 1, 2, and 3. Then, they were cultured for 12 days in the medium supplemented with different concentrations of PGE (0, 50, 100, and 500 µg/ mL, for control groups and groups 1, 2 and 3, respectively). At the end of th...
International Journal of Fertility & Sterility, 2018
Background There is some evidence indicating that Matricaria chamomile (MC) had protective effect... more Background There is some evidence indicating that Matricaria chamomile (MC) had protective effects on ischemia- reperfusion. In the present study, a rat model was used to investigate the effect of hydroalcoholic extract of MC on torsion/detorsion-induced testis tissue damage. Materials and Methods In this experimental study, 28 male Wistar rats were randomly divided into 4 groups as follows: G1, Sham operated; G2, testicular torsion/detorsion (T/D); G3, rats with testicular torsion/detorsion that received 300 mg/kg of MC extracts 30 minutes before detorsion (T/DMC); and G4, healthy rats that received 300 mg/kg of MC extracts (MC). Also, the reperfusion period was 24 hours. After blood sampling, the oxidative stress marker [e.g. superoxide dismutase (SOD) levels], blood levels of testosterone, and anti-oxidant enzyme levels [e.g. glutathione peroxidase (GPx)] were assessed by ELISA methods. Serum activity of malondialdehyde (MDA) was evaluated by spectrophotometry. Another assessment...
Archives of Medical Science, 2020
Introduction: To evaluate the effect of a herbal mixture (i.e., Mentha spicata, Zingiber officina... more Introduction: To evaluate the effect of a herbal mixture (i.e., Mentha spicata, Zingiber officinale, Cinnamomum zeylanicum, and Citrus sinensis) alone and in combination with clomiphene citrate (CC) compared to CC on the treatment of polycystic ovary syndrome (PCOS). Material and methods: This single-blind randomized clinical trial was conducted on 60 infertile participants with PCOS who were randomly divided into three groups. After spontaneous or progestin-induced withdrawal bleeding, group 1 (n = 20) received routine treatment with CC (50-150 mg) for three menstrual cycles from the 3 rd to 5 th day of menstruation for 5 days while group 2 (n = 20) and group 3 (n = 20) received herbal mixture capsules 700 mg/day and the herbal mixture along with CC for 3 months, respectively. Finally, several related parameters were measured, including the level of sex steroids, homeostatic model assessment for insulin resistance (HOMA-IR), lipid profile (primary outcomes), thyroid hormones, and clinical features. The analysis was based on intention-to-treat analysis. Results: No statistically significant differences were observed between the groups in terms of socio-demographic characteristics. However, after adjustment for baseline, luteinizing hormone (aMD = 4.9; 95% CI: 3.7-6.2), luteinizing hormone/follicle-stimulating hormone (aMD = 0.9; 95% CI: 0.7-1.2), total testosterone (aMD =-0.12; 95% CI:-0.2 to-0.01) in group 2 and free testosterone (aMD =-6.0; 95% CI:-9.7 to-2.3) in group 3 revealed a significant difference compared to group 1. In addition, HOMA-IR in group 2 (aMD =-1.3; 95% CI:-2.4 to-0.2) decreased significantly compared to group 1. Further, total cholesterol, triglycerides, low-density lipoprotein cholesterol, and very low-density lipoprotein cholesterol decreased significantly
Biomolecules, 2019
This paper aimed to evaluate the effect of herbal mixture (Mentha spicata, Zingiber officinale, C... more This paper aimed to evaluate the effect of herbal mixture (Mentha spicata, Zingiber officinale, Cinnamomum zeylanicum, and Citrus sinensis) only and along with clomiphene citrate (CC) compared to CC on serum antioxidants, glycemic status, menstrual regulation, and rate of pregnancy. This single-blind randomized clinical trial was carried out on 60 infertile participants with polycystic ovary syndrome (PCOS) willing to be pregnant. They were randomly allocated into group 1 (n = 20) who received routine dose of CC pills (50–150 mg) for three menstrual cycles from the fifth day of menstruation for five days; group 2 (n = 20) who consumed herbal mixture daily (700 mg); and group 3 (n = 20) who used up herbal mixture along with CC for 3 months. Catalase (CAT), glutathione peroxidase (GPx), superoxide dismutase (SOD), malondialdehyde (MDA), fasting blood sugar (FBS), insulin, and homeostatic model assessment for insulin resistance (HOMA-IR) were measured in their blood samples. No statist...
International Journal of Women's Health and Reproduction Sciences, 2015
Introduction Infertility is defined by the failure to achieve a clinical pregnancy after 12 month... more Introduction Infertility is defined by the failure to achieve a clinical pregnancy after 12 months or more of regular unprotected sexual intercourse which involves 10%-15% of couples. Male factor is responsible for 40% of infertility cases. The leading cause of male infertility is impaired sperm production caused by various factors such as infections, genetic disorders, medications, aging, social and environmental factors (1-5). Oxidative damage due to free radicals is one of the major causes of idiopathic oligospermia, even with undesirable impact on the structure of sperm produces abnormal sperm. For example, in diabetic patients by producing reactive oxygen species (ROS), free radicals of nitric oxide cause oxidative stress and thus result in testicular insufficiency through affecting spermatogenesis and estrogenic pathways (6-8). Varicocele which has been observed in 19% to 41% of infertile men is a common cause of male infertility (9-11). Increased levels of ROS and oxidative levels (oxidative stress) is one of the proposed mechanisms for impaired spermatogenesis. Vacuole formation in testis tissue is a sign of oxidative stress and is associated with aging which can be neutralized by antioxidants (12-15). Lack of vitamin A, elements such as flavonoids, carnitine, folate, zinc and selenium and overall reduction of antioxidants in the diet can be one of the reasons for infertility especially oligospermia and asthenozoospermia in humans (16-18). Antioxidants like vitamin E, vitamin C, glutathione, coenzyme Q10 and natural polyphenols such as flavonoids and phenolic acids by reducing the damage caused by free radicals, strengthening blood-testis barrier and protecting and repairing sperm DNA, are effective in the treatment of male infertility (19-21). Assisted reproductive techniques (ART) such as in vitro fertilization (IVF), zygote intra-fallopian transfer (ZIFT) and intra-cytoplasmic sperm insemination (ICSI) has solved many problems of infertility; however, due to high cost, they are not affordable by many of infertile couples. On the other hand, there are effective medications which are less expensive and available for a wide range of couples. The bioactive compounds in plant extracts are highly regarded because it is derived from natural sources and compatible with vital systems. Using herbal medicine to increase fertility and improve factors such as hormonal imbalance, oligospermia, low motility of sperm, prostatitis, varicocele, etc has been considered for a long time. Previous studies on laboratory animals indicated that plants such as Allium cepa, Zingiber officinale, Ocimum basilicum, Cinnamomum verum, Citrus sinensis' peel, Citrullus lanatus and Daucus carota' seeds as a rich source of
International Journal of Women's Health and Reproduction Sciences, 2014
Objectives: Polycystic ovary syndrome (PCOS) is the most frequent cause of female infertility, af... more Objectives: Polycystic ovary syndrome (PCOS) is the most frequent cause of female infertility, affecting about 4% to 8% of women in the age of procreation. For evaluation the protective effects of omega-3 polyunsaturated fatty acid on ovarian structure in experimental PCO induced by estradiol-valerat, this research was done. Materials and Methods: Wistar female rats (n=40) were allocated into four groups, one control (n=10) and three test groups (n=30), that one group received omega-3 (60 mg/rat/orally/daily), second and third groups were induced PCO by single injection of estradiol-valerate (16mg/ kg/ i.m), third group also received omega-3 (240 mg/kg) for 60 consequence days. Animals were kept in standard conditions. On day 60, the ovarian tissue of Rats in whole groups were removed and prepared for pathological analysis. Results: vacuolated area and rough endoplasmic reticulum expanded, de-granulated, disorganized were seen in PCO groups; however, these side effects decreased in the groups that received omega-3 significantly (p<0.05) in comparison to experiment groups and ovarian weights in PCO experimental decreased significantly (p<0.05). Conclusion: Results revealed that administration of omega-3 could significantly treat PCO. This suggested that polyunsaturated fatty acid could diminish negative side effects of PCO on ovary tissue.
Journal of Obstetrics and Gynaecology, 2021
Abstract The current study was designed to investigate the effect of eugenol on histopathological... more Abstract The current study was designed to investigate the effect of eugenol on histopathological changes and oxidative stress caused by torsion/detorsion in the ovary of adult female rats. In this study, forty-eight female Wistar rats were classified into six groups; Sham and 2 healthy group receiving 30, 60 mg/kg eugenol; ovarian torsion/detorsion; and 2 experimental groups receiving 30, 60 mg/kg eugenol. After ten days, the plasma levels of oestrogen, testosterone, and some oxidative stress markers were assessed. also, the histomorphometric study was performed. A marked degenerative changes in the TD group was observed (p < .001). The oestrogen, GPX, and SOD levels were remarkably declined in the G2 group, while they were reversed to the baseline values in groups receiving eugenol. The concentration of malondialdehyde (MDA) was remarkably increased during the ischaemia (p < .001). The treatment with eugenol significantly diminished MDA levels in different groups (p < .001). Our finding indicated that eugenol could protect the ovarian tissue against oxidative stress and tissue injury induced by torsion/detorsion. IMPACT STATEMENT What is already known about this subject? Ovarian torsion is one of the commonest gynecological emergencies in all age groups of the female gender. Timely diagnosis and management of ovarian torsion are crucial, especially for women of reproductive age. Detorsion is one of the interventions used for the prevention of ovarian tissue damage. Ovarian ischaemia/reperfusion is a pathophysiological condition in which decreased blood flow, and oxygen deficiency (ischaemia) are observed in ovarian tissues as a result of ovarian torsion. Following torsion, the inflammatory response induced by detorsion (reperfusion) leads to vascular endothelial cell apoptosis and microcirculation abnormalities, which are responsible for the cause of ovarian tissue damage. What do the results of this study add? This study found that eugenol, an antioxidant and anti-inflammatory agent, could be used experimentally to diminish the I/R damage in the ovary through the attenuation of detrimental histological events, decreasing the serum level of MDA and testosterone, and increasing the level of SOD and GPX enzymes. To date, there is no report on the application of eugenol for diminishing T/D-induced oxidative stress in the ovary. What are the implications of these findings for clinical practice? Eugenol has been shown to possess therapeutic properties in patients with ovarian torsion. Further clinical studies are necessary to prove the beneficial effect of eugenol on the prevention of I/R-induced ovarian damage.
Objectives: Rupture of membranes prior to the onset of labor is known as Premature Rupture of Mem... more Objectives: Rupture of membranes prior to the onset of labor is known as Premature Rupture of Membranes ( PROM). Early and correct diagnosis is crucial in order to prevent fetal and maternal risks that can be life threatening. We aimed to investigate the diagnostic ability of the tests in PROM. Materials and Methods: Nitrazine test, fern test, amnio-dye test, biochemical tests (insulin-like growth factor binding protein-1 and placental alpha microglobulin-1) were evaluated in terms of effectiveness in diagnosis of PROM. Results: A gold standard method has not yet been defined in PROM. Diagnostic tests (nitrazine test, fern test, Insulin-like Growth Factor Binding Protein-1 IGFBP-1 and Placental Alpha Microglobulin-1 PAMG-1) should be used when the diagnosis is not certain following history, examination with sterile speculum and ultrasonography evaluation. Conclusion: IGFBP-1 and PAMG-1 are tests based on bedside immunochromatographic method. Especially, PAMG-1 comes into prominence ...
Academic Journals, Apr 30, 2009
The aim of this study was to investigate the comparative effects of aminoglycosides and fluoroqui... more The aim of this study was to investigate the comparative effects of aminoglycosides and fluoroquinolones on testis tissue in rats. Thirty male Wister rats were randomly divided into control (n=10) and experimental (n=20) groups. The experimental groups subdivided into two groups of ten. Each received 5 mg/kg (IP) Gentamicin and 72 mg/kg (IP) Ofloxacin daily for 14 days, respectively; however, the control group just received vehicle (IP). In the fourteenth day, rats were killed and testis tissues were also prepared for light and electron microscopic study.Allanimalsexposedtodrugswereseenasdepletion of germ cells, germinal cells necrosis, especially in spermatogonia and Leydig cells had an abnormal fibroblast-like appearance, abnormal space between neighbor sertoli cells, mitochondria were lost cristae and vacuolated (none energized), lyzosome seen more in cytoplasm of sertoli cells, nucleus of moiled cell was done heterochromatin. Gentamicin and Ofloxacin have negative effects on testis architecture and germinal cells damagesn rats. Therefore, it is recommended that usage of this drug have harmful side effects on male fertility.
Background and Objectives: Ciprofloxacin is a synthetic antibacterial agent belonging to Fluoroqu... more Background and Objectives: Ciprofloxacin is a synthetic antibacterial agent belonging to Fluoroquinolone drugs affecting effectively on gram-negative bacterial infectious. The aim of this study was to assess the effect of ciprofloxacin in the spermatogenesis period.
Anti-oxidative effects of citro flavonoids on spermatogenesis in rat