Susheel Kumar | TERI University (original) (raw)

Papers by Susheel Kumar

Research paper thumbnail of Identification of antifungal principle in the solvent extract of an endophytic fungus Chaetomium globosum, from Withania somnifera

SpringerPlus, Feb 6, 2013

Extracts of Chaetomium globosum EF18, isolated as endophytic fungus from Withania somnifera, were... more Extracts of Chaetomium globosum EF18, isolated as endophytic fungus from Withania somnifera, were found effective against Sclerotinia sclerotiorum. Ethyl acetate and methanol extracts were more effective than hexane extract showing >80% growth inhibition. Bioactive compound (antibiotic Sch 210971, m/z 445 and lamdamax 290) having antifungal activity against S. sclerotiorum has been isolated in pure form from the ethyl acetate extract following bioassay guided fractionation. Apart from this compound other fractions of polar to medium polarity were also found effective. Fraction no. VIII from VLC (Vacuum liquid chromatography) column of ethyl acetate extract was most active having IC50 value 35.4 mug/ml.

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Research paper thumbnail of Endophytic Fungi Isolated from Oil-Seed Crop Jatropha curcas Produces Oil and Exhibit Antifungal Activity

PLoS ONE, Feb 8, 2013

Jatropha curcas L., a perennial plant grown in tropics and subtropics is popularly known for its ... more Jatropha curcas L., a perennial plant grown in tropics and subtropics is popularly known for its potential as biofuel. The plant is reported to survive under varying environmental conditions having tolerance to stress and an ability to manage pest and diseases. The plant was explored for its endophytic fungi for use in crop protection. Endophytic fungi were isolated from leaf of Jatropha curcas, collected from New Delhi, India. Four isolates were identified as Colletotrichum truncatum, and other isolates were identified as Nigrospora oryzae, Fusarium proliferatum, Guignardia cammillae, Alternaria destruens, and Chaetomium sp. Dual plate culture bioassays and bioactivity assays of solvent extracts of fungal mycelia showed that isolates of Colletotrichum truncatum were effective against plant pathogenic fungi Fusarium oxysporum and Sclerotinia sclerotiorum. Isolate EF13 had highest activity against S. sclerotiorum. Extracts of active endophytic fungi were prepared and tested against S. sclerotiorum. Ethyl acetate and methanol extract of C. truncatum EF10 showed 71.7% and 70% growth inhibition, respectively. Hexane extracts of C. truncatum isolates EF9, EF10, and EF13 yielded oil and the oil from EF10 was similar to oil of the host plant, i.e., J. curcas.

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Research paper thumbnail of Metabolites of endophytic fungi as novel source of biofungicide: a review

Phytochemistry Reviews, Jan 2013

Endophytic fungi are known to harbour compound(s) beneficial for plant health as well as human he... more Endophytic fungi are known to harbour compound(s) beneficial for plant health as well as human health. Among the metabolites of agrochemical and pharmaceutical importance alkaloids are the major. Apart from alkaloids several terpernoids and steroids, isocoumarins and chromones, phenolics and volatiles have also been reported. Cryptocin and cytochalasins alkaloids were isolated during early phase of innovation and proved to be antifungal. Some of these metabolites produced by endophytic fungi were originally isolated from the host plants.

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Research paper thumbnail of Endophytic fungi for pest and disease management

… of Diseases Caused by …, Jan 1, 2008

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Research paper thumbnail of Isolation, characterization, and bioactivity of endophytic fungi of Tylophora indica

World Journal of …

Dothediomycetes sp., Alternaria tenuissima, Thielavia subthermophila, Alternaria sp., Nigrospora ... more Dothediomycetes sp., Alternaria tenuissima, Thielavia subthermophila, Alternaria sp., Nigrospora oryzae, Colletotrichum truncatum, and Chaetomium sp. were isolated as endophytic fungi from leaves and stems of the medicinal plant, Tylophora indica, based on rDNA sequencing of ITS region and microscopic examination. Alternaria tenuissima, Colletotrichum truncatum, and Alternaria sp. were found to be active against both Sclerotinia sclerotiorum and Fusarium oxysporum. Chaetomium sp. showed very mild activity against Sclerotinia sclerotiorum and Fusarium oxysporum. Whereas Dothideomycete sp. and Thielavia subthermophila showed high activity against Sclerotinia sclerotiorum. Methanol extract of Dothediomycetes sp. showed 66.5% growth inhibition (GI) at 500 μg/ml.

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Research paper thumbnail of Identification of antifungal principle in the solvent extract of an endophytic fungus Chaetomium globosum, from Withania somnifera

SpringerPlus, Feb 6, 2013

Extracts of Chaetomium globosum EF18, isolated as endophytic fungus from Withania somnifera, were... more Extracts of Chaetomium globosum EF18, isolated as endophytic fungus from Withania somnifera, were found effective against Sclerotinia sclerotiorum. Ethyl acetate and methanol extracts were more effective than hexane extract showing >80% growth inhibition. Bioactive compound (antibiotic Sch 210971, m/z 445 and lamdamax 290) having antifungal activity against S. sclerotiorum has been isolated in pure form from the ethyl acetate extract following bioassay guided fractionation. Apart from this compound other fractions of polar to medium polarity were also found effective. Fraction no. VIII from VLC (Vacuum liquid chromatography) column of ethyl acetate extract was most active having IC50 value 35.4 mug/ml.

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Research paper thumbnail of Endophytic Fungi Isolated from Oil-Seed Crop Jatropha curcas Produces Oil and Exhibit Antifungal Activity

PLoS ONE, Feb 8, 2013

Jatropha curcas L., a perennial plant grown in tropics and subtropics is popularly known for its ... more Jatropha curcas L., a perennial plant grown in tropics and subtropics is popularly known for its potential as biofuel. The plant is reported to survive under varying environmental conditions having tolerance to stress and an ability to manage pest and diseases. The plant was explored for its endophytic fungi for use in crop protection. Endophytic fungi were isolated from leaf of Jatropha curcas, collected from New Delhi, India. Four isolates were identified as Colletotrichum truncatum, and other isolates were identified as Nigrospora oryzae, Fusarium proliferatum, Guignardia cammillae, Alternaria destruens, and Chaetomium sp. Dual plate culture bioassays and bioactivity assays of solvent extracts of fungal mycelia showed that isolates of Colletotrichum truncatum were effective against plant pathogenic fungi Fusarium oxysporum and Sclerotinia sclerotiorum. Isolate EF13 had highest activity against S. sclerotiorum. Extracts of active endophytic fungi were prepared and tested against S. sclerotiorum. Ethyl acetate and methanol extract of C. truncatum EF10 showed 71.7% and 70% growth inhibition, respectively. Hexane extracts of C. truncatum isolates EF9, EF10, and EF13 yielded oil and the oil from EF10 was similar to oil of the host plant, i.e., J. curcas.

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Research paper thumbnail of Metabolites of endophytic fungi as novel source of biofungicide: a review

Phytochemistry Reviews, Jan 2013

Endophytic fungi are known to harbour compound(s) beneficial for plant health as well as human he... more Endophytic fungi are known to harbour compound(s) beneficial for plant health as well as human health. Among the metabolites of agrochemical and pharmaceutical importance alkaloids are the major. Apart from alkaloids several terpernoids and steroids, isocoumarins and chromones, phenolics and volatiles have also been reported. Cryptocin and cytochalasins alkaloids were isolated during early phase of innovation and proved to be antifungal. Some of these metabolites produced by endophytic fungi were originally isolated from the host plants.

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Research paper thumbnail of Endophytic fungi for pest and disease management

… of Diseases Caused by …, Jan 1, 2008

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Research paper thumbnail of Isolation, characterization, and bioactivity of endophytic fungi of Tylophora indica

World Journal of …

Dothediomycetes sp., Alternaria tenuissima, Thielavia subthermophila, Alternaria sp., Nigrospora ... more Dothediomycetes sp., Alternaria tenuissima, Thielavia subthermophila, Alternaria sp., Nigrospora oryzae, Colletotrichum truncatum, and Chaetomium sp. were isolated as endophytic fungi from leaves and stems of the medicinal plant, Tylophora indica, based on rDNA sequencing of ITS region and microscopic examination. Alternaria tenuissima, Colletotrichum truncatum, and Alternaria sp. were found to be active against both Sclerotinia sclerotiorum and Fusarium oxysporum. Chaetomium sp. showed very mild activity against Sclerotinia sclerotiorum and Fusarium oxysporum. Whereas Dothideomycete sp. and Thielavia subthermophila showed high activity against Sclerotinia sclerotiorum. Methanol extract of Dothediomycetes sp. showed 66.5% growth inhibition (GI) at 500 μg/ml.

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