Dharmawaty M. Taher | Institut Pertanian Bogor (IPB) (original) (raw)

Papers by Dharmawaty M. Taher

Research paper thumbnail of Potensi cengkeh (Syzygium aromaticum) varietas Afo sebagai larvasida alami nyamuk Anopheles subpictus dan Aedes aegypti

Prosiding Seminar Nasional Masyarakat Biodiversitas Indonesia, Sep 1, 2015

Mer & Perry) varietas afo dikenal sebagai cengkeh tertua di dunia karena mencapai usia ratusan ta... more Mer & Perry) varietas afo dikenal sebagai cengkeh tertua di dunia karena mencapai usia ratusan tahun dan tumbuh di kawasan pegunungan Gamalama Ternate Maluku Utara. Kandungan minyak atsiri daun cengkeh afo sebesar 3.20% dan kadar eugenol 90.53% dapat dimanfaatkan sebagai larvasida alami. Pengendalian vektor nyamuk Anopheles sp. dan Aedes aegypti adalah salah satu langkah pengendalian penting untuk menanggulangi bahaya penyakit malaria dan (DBD) di Indonesia. Penelitian ini bertujuan untuk mengetahui efektivitas daun cengkeh varietas afo terhadap kematian larva nyamuk Anopheles subpictus dan Ae. aegypti. Metode penelitian adalah eksperimen dengan Rancangan Acak Lengkap (RAL). Larva nyamuk An. subpictus instar III sejumlah 330 ekor dibagi menjadi 10 perlakuan ekstrak air daun cengkeh (10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90% dan 100%) ditambah 1 kontrol dengan 3 kali ulangan. Setiap perlakuan berisi 10 ekor larva dan diamati setiap selang waktu 5 menit selama 30 menit. Pengujian larva Ae. aegypti instar III menggunakan ekstrak air dalam bentuk infusa daun cengkeh. Jumlah larva yang digunakan sebanyak 400 ekor dengan 4 perlakuan (5%, 10%, 15% dan 20%) ditambah 1 kontrol. Masing-masing kelompok perlakuan berisi 20 ekor larva dan dilakukan 4 kali ulangan. Pengamatan dilakukan setiap selang waktu 3 jam selama 15 jam dan dihitung jumlah larva yang mati. Hasil penelitian menunjukan bahwa efektivitas ekstrak daun cengkeh var. Afo terhadap larva An. subpictus terjadi pada menit ke 10 setelah perlakuan dengan konsentrasi 80%, tingkat kematian mencapai 33,3% dan semakin meningkat seiring lamanya waktu aplikasi. Konsentrasi infusa daun cengkeh 20% efektif terhadap kematian larva nyamuk Ae. aegypti pada 3 jam pertama aplikasi, dan mencapai kematian larva 85% pada aplikasi selama 15 jam. Cengkeh varietas Afo berpotensi sebagai larvasida alami untuk penanggulangan vektor nyamuk malaria dan DBD.

Research paper thumbnail of Case method to increase student participation and problem-solving skills in biochemistry subject lesson study integrated at Khairun University

Bio-Inoved : Jurnal Biologi-Inovasi Pendidikan, Feb 16, 2023

The Biochemistry course is a basic and compulsory subject for Biology Education students. This st... more The Biochemistry course is a basic and compulsory subject for Biology Education students. This study aims to determine students' level of participation and problem-solving skills through the ability to solve cases of Biomolecules and proteins on Student Worksheets (LKM). This is an Action Research integrated with lesson study with two cycles. The instruments used were student participation rubrics in the case method and LKM problemsolving skills in the Biochemistry course. The subjects in this study were 24 third-semester students of the Biology Education Study Program. Data analysis techniques through descriptive statistics with percentages. The results showed an increase in the percentage from cycle 1 to cycle 2. Student participation in collaborative activities increased from 71.5% to 73.3%, while problem-solving skills increased from 53.5% to 71.7%. Based on the observer transcripts, some students still do not understand the material and cases discussed in lectures in class and the laboratory. This is because students do not actively ask questions and do not have curiosity about the material and case explanations presented by lecturers or fellow students. This study shows that implementing the integrated case method in lesson study can increase student participation activities and problem-solving skills in Biochemistry courses.

Research paper thumbnail of Bioassay and efficacy of clove flower stalk extract of Afo variety as a natural larvicidal

International Journal of Biological and Pharmaceutical Sciences Archive, Mar 30, 2023

This study aims to test the larvicidal bioassay of clove stalk extract against Aedes aegypti on a... more This study aims to test the larvicidal bioassay of clove stalk extract against Aedes aegypti on a laboratory scale and field scale efficacy test in a bath. Samples of sun-dried clove flower stalks were made into water extracts and coarse powder measuring 8-10 mesh for efficacy tests in the bath. The experimental method with a completely randomized design model was used to test the effectiveness of the larvicide, which consisted of two stages: The aqueous extract was tested against Ae. aegypti third instar larvae for three concentrations, namely 10%, 20%, and 30%, plus control treatment with three repetitions; observations were made at 30 minutes and 60 minutes after application to see the number of dead larvae as stage one. The second stage was the efficacy test in the bath which was carried out by inserting clove coarse powder in packs of 20 grams, 30 grams, and 40 grams, as well as the control treatment with five replicates, observed every day to see the time of emergence of mosquito larvae in a matter of days. The results of the first stage test showed an effect of the concentration of clove flower stalk extract on the mortality rate of the larvae, which was indicated by a positive relationship, where the mortality rate of the larvae reached 100% at 60 minutes after application. In comparison, the second stage test showed that there was an effect of clove flower stalk coarse powder on the time of larvae emergence, where the average time for larvae emergence was up to 8.8 days in the treatment of 40 g of clove flower stalk coarse powder, 1.83 times longer than the control (4 .8 days). Clove flower stalk extract is effective as a larvicidal mosquito Ae. aegypti.

Research paper thumbnail of Pengaruh Model Pembelajaran Talking Stick Terintegrasi Saintifik Berbantuan Media Charta Terhadap Hasil Belajar Kognitif Peserta Didik Kelas VIII SMP Negeri 16 Halmahera Selatan

JURNAL BIOEDUKASI

The scientifically integrated talking stick learning model assisted by charta media is a learning... more The scientifically integrated talking stick learning model assisted by charta media is a learning stage following the steps of the talking stick learning model with charta-assisted media to improve the ability of students' cognitive learning outcomes. This study aims to determine the effect of the scientifically integrated talking stick learning model assisted by chart media on students' cognitive learning outcomes. This research is a quasi-experimental research (Quasy Experiment). The sample of this study consisted of two classes, namely class VIII-1 as the experimental class and VIII-2 as the control class with a total of 35 students. The instrument used is a test to measure cognitive learning outcomes. Data analysis used the SPSS-assisted normality and homogeneity test, while the hypothesis test used the Anacova test. The results showed that the talking stick learning model with chart media had an effect on learning outcomes with a significant level of 0.05 0.001.

Research paper thumbnail of Case method to increase student participation and problem-solving skills in biochemistry subject lesson study integrated at Khairun University

BIO-INOVED : Jurnal Biologi-Inovasi Pendidikan

The Biochemistry course is a basic and compulsory subject for Biology Education students. This st... more The Biochemistry course is a basic and compulsory subject for Biology Education students. This study aims to determine students' level of participation and problem-solving skills through the ability to solve cases of Biomolecules and proteins on Student Worksheets (LKM). This is an Action Research integrated with lesson study with two cycles. The instruments used were student participation rubrics in the case method and LKM problem-solving skills in the Biochemistry course. The subjects in this study were 24 third-semester students of the Biology Education Study Program. Data analysis techniques through descriptive statistics with percentages. The results showed an increase in the percentage from cycle 1 to cycle 2. Student participation in collaborative activities increased from 71.5% to 73.3%, while problem-solving skills increased from 53.5% to 71.7%. Based on the observer transcripts, some students still do not understand the material and cases discussed in lectures in class...

Research paper thumbnail of Bioassay and efficacy of clove flower stalk extract of Afo variety as a natural larvicidal

International Journal of Biological and Pharmaceutical Sciences Archive

This study aims to test the larvicidal bioassay of clove stalk extract against Aedes aegypti on a... more This study aims to test the larvicidal bioassay of clove stalk extract against Aedes aegypti on a laboratory scale and field scale efficacy test in a bath. Samples of sun-dried clove flower stalks were made into water extracts and coarse powder measuring 8-10 mesh for efficacy tests in the bath. The experimental method with a completely randomized design model was used to test the effectiveness of the larvicide, which consisted of two stages: The aqueous extract was tested against Ae. aegypti third instar larvae for three concentrations, namely 10%, 20%, and 30%, plus control treatment with three repetitions; observations were made at 30 minutes and 60 minutes after application to see the number of dead larvae as stage one. The second stage was the efficacy test in the bath which was carried out by inserting clove coarse powder in packs of 20 grams, 30 grams, and 40 grams, as well as the control treatment with five replicates, observed every day to see the time of emergence of mosqu...

Research paper thumbnail of Pengembangan Perangkat Pembelajaran Biologi Berbasis Inkuiri Terbimbing Terintegrasi Saintifik Serta Pengaruhnya Terhadap Hasil Belajar Kognitif Dan Keterampilan Proses Sains Pada Siswa Kelas XI Sma Negeri 3 Halmahera Selatan

EDUKASI

The purpose of this study was (1) to determine whether the scientifically integrated guided inqui... more The purpose of this study was (1) to determine whether the scientifically integrated guided inquiry-based Biology learning device that was developed could be declared feasible to be used in class XI biology learning at SMA Negeri 3 Halmahera Selatan (2) to determine the effect of the scientifically integrated Guided Inquiry learning model on learning outcomes. cognitive skills in class XI SMA Negeri 3 South Halmahera (3) to determine the effect of the scientifically integrated Guided Inquiry learning model on science process skills in class XI students at SMA Negeri 3 Halmahera Selatan. This research is a research development or Research and Development (R&D). The sample of this study consisted of two classes, namely XI IPA-1 as an experimental class and Class XI IPA-2 as a control class with 36 students. The instrument used was an essay test for science process skills and for cognitive learning outcomes in the form of a multiple choice test (PG). ). Analysis of the data using data ...

Research paper thumbnail of Potensi cengkeh (Syzygium aromaticum) varietas Afo sebagai larvasida alami nyamuk Anopheles subpictus dan Aedes aegypti Potention of clove (Syzygium aromaticum) Afo variety as natural mosquito larvicidal of Anopheles subpictus and Aedes aegypti

Mer & Perry) varietas afo dikenal sebagai cengkeh tertua di dunia karena mencapai usia ratusan ta... more Mer & Perry) varietas afo dikenal sebagai cengkeh tertua di dunia karena mencapai usia ratusan tahun dan tumbuh di kawasan pegunungan Gamalama Ternate Maluku Utara. Kandungan minyak atsiri daun cengkeh afo sebesar 3.20% dan kadar eugenol 90.53% dapat dimanfaatkan sebagai larvasida alami. Pengendalian vektor nyamuk Anopheles sp. dan Aedes aegypti adalah salah satu langkah pengendalian penting untuk menanggulangi bahaya penyakit malaria dan (DBD) di Indonesia. Penelitian ini bertujuan untuk mengetahui efektivitas daun cengkeh varietas afo terhadap kematian larva nyamuk Anopheles subpictus dan Ae. aegypti. Metode penelitian adalah eksperimen dengan Rancangan Acak Lengkap (RAL). Larva nyamuk An. subpictus instar III sejumlah 330 ekor dibagi menjadi 10 perlakuan ekstrak air daun cengkeh (10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90% dan 100%) ditambah 1 kontrol dengan 3 kali ulangan. Setiap perlakuan berisi 10 ekor larva dan diamati setiap selang waktu 5 menit selama 30 menit. Pengujian larva Ae. aegypti instar III menggunakan ekstrak air dalam bentuk infusa daun cengkeh. Jumlah larva yang digunakan sebanyak 400 ekor dengan 4 perlakuan (5%, 10%, 15% dan 20%) ditambah 1 kontrol. Masing-masing kelompok perlakuan berisi 20 ekor larva dan dilakukan 4 kali ulangan. Pengamatan dilakukan setiap selang waktu 3 jam selama 15 jam dan dihitung jumlah larva yang mati. Hasil penelitian menunjukan bahwa efektivitas ekstrak daun cengkeh var. Afo terhadap larva An. subpictus terjadi pada menit ke 10 setelah perlakuan dengan konsentrasi 80%, tingkat kematian mencapai 33,3% dan semakin meningkat seiring lamanya waktu aplikasi. Konsentrasi infusa daun cengkeh 20% efektif terhadap kematian larva nyamuk Ae. aegypti pada 3 jam pertama aplikasi, dan mencapai kematian larva 85% pada aplikasi selama 15 jam. Cengkeh varietas Afo berpotensi sebagai larvasida alami untuk penanggulangan vektor nyamuk malaria dan DBD.

Research paper thumbnail of Pengujian Antimalaria Menggunakan Tanaman Cengkeh (Syzygium aromaticum (L.) Merr & Perry) (Studi in vitro, in vivo, Toksisitas dan Screening Senyawa Aktif)

Research paper thumbnail of ANTIMALARIAL ACTIVITY OF METHANOL EXTRACT OF CLOVE FLOWER AND CLOVE STALK FLOWER OF TUNI BURU SELATAN VARIETIES ON Plasmodium berghei

The methanol extract of clove flower and clove flower stalk varieties of tuni Buru selatan were e... more The methanol extract of clove flower and clove flower stalk varieties of tuni Buru selatan were evaluated for in vivo antimalarial activity, in a 4-day suppression test against Plasmodium berghei in mice. The in vivo model for testing the antimalarial effects of plant extracts is described. Selected mice (20-30 g body weight) were divided into 4 groups, each consisting of 5 mice. Each animal is injected with red blood cells Plasmodium berghei. Testing was done by Peter test, mice were given oral treatment with diluted content 25 mg / kg bw, 50 mg / kg bw and 100 mg / kg bw for 4 groups. One group served as negative control. Negative controls were given 0.5% CMC Na solution. ED50 extract methanol of clove flower and clove stalk flower varieties of Tuni Buru selatan in Plasmodium berghei mice were 39,96 and 20,023 mg / kg bw. Keywords: antimalarial, clove flower, clove flower stalk, Plasmodium berghei

Research paper thumbnail of Pendampingan pembuatan hand sanitizer berbasis kearifan lokal (ekstrak tangkai bunga cengkeh)

ABSYARA: Jurnal Pengabdian Pada Masyarakat, 2020

Efforts to prevent viral infection are a shared responsibility of all Indonesian citizens, includ... more Efforts to prevent viral infection are a shared responsibility of all Indonesian citizens, including academics. Prevention starts from keeping a distance, wearing a mask, and washing hands. Another alternative to washing hands is to use a hand sanitizer. This community service activity program aims to educate and assist the community in preventing infection with the Covid-19 virus based on local culture through assisting students in making hand sanitizers made from local products, namely clove flower stem extract, and distributing them to the community. The methods used in this activity are production, product distribution, and education (lectures). The team will distribute the product to the Khairun University campus area and six schools in Ternate city. This community service program's result is 300 bottles of clove flower stalk extract and distributed to the public. In general, the community's response to the hand sanitizer product from clove flower stalk extract is that ...

Research paper thumbnail of Ekstrak Metanol Cengkeh (Syzygium Aromaticum (L.) Merry & Perry) Varietas Tuni Buru Selatan sebagai Antimalaria

Acta VETERINARIA Indonesiana, 2018

Malaria masih menjadi salah satu masalah kesehatan di dunia dan Indonesia. Cengkeh (Syzygium arom... more Malaria masih menjadi salah satu masalah kesehatan di dunia dan Indonesia. Cengkeh (Syzygium aromaticum (L.) Merry…

Research paper thumbnail of Pengetahuan Lokal Masyarakat Kepulauan Tentang Tumbuhan Anti-nyamuk

Techno: Jurnal Penelitian, 2020

Pemanfaatan bahan alami dari tanaman lokal sebagai anti-nyamuk dimulai dari pendalaman informasi ... more Pemanfaatan bahan alami dari tanaman lokal sebagai anti-nyamuk dimulai dari pendalaman informasi tentang pengetahuan lokal masyarakat. Upaya ini perlu dilakukan untuk mengatasi beberapa permasalahan dari penggunaan anti-nyamuk sintetik yang beredar di pasaran, juga sebagai bagian dari upaya melestarikan kearifan lokal masyarakat yang mulai tergerus oleh kemajuan zaman. Tujuan penelitian ini adalah untuk mendeskripsikan pengetahuan lokal masyarakat kepulauan tentang pemanfaatan tumbuhan anti-nyamuk dan menganalisis potensi tumbuhan yang paling banyak digunakan masyarakat di wilayah kepulauan sebagai produk lotion anti-nyamuk. Metode yang digunakan adalah metode survei eksploratif. Pengumpulan data dilakukan dengan teknik wawancara, observasi secara mendalam terhadap informan dan dokumentasi. Data jenis tumbuhan anti-nyamuk yang paling banyak digunakan dianalisis komponen fitokimia. Hasil penelitian menunjukkan terdapat 9 jenis tumbuhan anti-nyamuk yang digunakan masyarakat yaitu Tagetes erecta L., Lavandula L., Cymbopogon citratus, Citrus aurantifolia, Syzygium aromaticum, Evodia suaveolens, Catharanthus roseus (L) G. Don., Alphitonia incana BL. dan Polygala paniculata. Jenis Alphitonia incana BL paling banyak digunakan masyarakat dan mengandung senyawa fitokimia alkaloid, terpenoid, flavonoid, fenolik dan tanin. Jenis Alphitonia incana BL berpotensi untuk dikembangkan sebagai produk lotion anti-nyamuk.

Research paper thumbnail of Serological and molecular prevalence of equine piroplasmosis in Western Java, Indonesia

Veterinary Parasitology: Regional Studies and Reports, 2018

Equine piroplasmosis is an economically significant disease caused by Theileria equi and Babesia ... more Equine piroplasmosis is an economically significant disease caused by Theileria equi and Babesia caballi, which are tick-borne hemoprotozoan parasites. Infections with these parasite species had never been reported in horses in Indonesia. The aim of the present study was to investigate the prevalence of T. equi and B. caballi in horses reared in parts of Western Java, Indonesia. Blood samples were collected randomly from 235 horses in four different districts (Bandung, Depok, Tangerang, and Bogor) in Western Java, Indonesia. Thin blood smears prepared from the sampled animals were stained by Giemsa and observed under a light microscope. Serum samples prepared from blood were screened by enzyme-linked immunosorbent assays (ELISAs) based on recombinant forms of EMA-2 and BC48 antigens to determine the seroprevalence of T. equi and B. caballi, respectively. DNA samples extracted from the same blood samples were screened by EMA-2 and BC48 gene-based nested polymerase chain reaction (nPCR) assays for T. equi and B. caballi infections, respectively. Of 235 surveyed animals, five (2.1%) and 15 (6.4%) were seropositive for T. equi and B. caballi, respectively, whereas one and four horses were nPCR-positive for T. equi and B. caballi, respectively. All of the surveyed animals were negative for T. equi and B. caballi by microscopy. The T. equi EMA-2 and B. caballi BC48 gene fragments amplified by the nPCR assays were cloned, sequenced, and subjected to bioinformatic and phylogenetic analyses. The T. equi EMA-2 gene sequence from an Indonesian horse was identical to sequences from Florida and Washington strains and clustered together with these sequences in phylogeny. On the other hand, four Indonesian BC48 gene sequences shared 99.8-100% identity scores. This present study is the first to report T. equi and B. caballi in horses in Indonesia. Our findings highlight the need for monitoring horses in Indonesia for clinical piroplasmosis caused by T. equi and B. caballi.

Research paper thumbnail of Potensi cengkeh (Syzygium aromaticum) varietas Afo sebagai larvasida alami nyamuk Anopheles subpictus dan Aedes aegypti

Prosiding Seminar Nasional Masyarakat Biodiversitas Indonesia, Sep 1, 2015

Mer & Perry) varietas afo dikenal sebagai cengkeh tertua di dunia karena mencapai usia ratusan ta... more Mer & Perry) varietas afo dikenal sebagai cengkeh tertua di dunia karena mencapai usia ratusan tahun dan tumbuh di kawasan pegunungan Gamalama Ternate Maluku Utara. Kandungan minyak atsiri daun cengkeh afo sebesar 3.20% dan kadar eugenol 90.53% dapat dimanfaatkan sebagai larvasida alami. Pengendalian vektor nyamuk Anopheles sp. dan Aedes aegypti adalah salah satu langkah pengendalian penting untuk menanggulangi bahaya penyakit malaria dan (DBD) di Indonesia. Penelitian ini bertujuan untuk mengetahui efektivitas daun cengkeh varietas afo terhadap kematian larva nyamuk Anopheles subpictus dan Ae. aegypti. Metode penelitian adalah eksperimen dengan Rancangan Acak Lengkap (RAL). Larva nyamuk An. subpictus instar III sejumlah 330 ekor dibagi menjadi 10 perlakuan ekstrak air daun cengkeh (10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90% dan 100%) ditambah 1 kontrol dengan 3 kali ulangan. Setiap perlakuan berisi 10 ekor larva dan diamati setiap selang waktu 5 menit selama 30 menit. Pengujian larva Ae. aegypti instar III menggunakan ekstrak air dalam bentuk infusa daun cengkeh. Jumlah larva yang digunakan sebanyak 400 ekor dengan 4 perlakuan (5%, 10%, 15% dan 20%) ditambah 1 kontrol. Masing-masing kelompok perlakuan berisi 20 ekor larva dan dilakukan 4 kali ulangan. Pengamatan dilakukan setiap selang waktu 3 jam selama 15 jam dan dihitung jumlah larva yang mati. Hasil penelitian menunjukan bahwa efektivitas ekstrak daun cengkeh var. Afo terhadap larva An. subpictus terjadi pada menit ke 10 setelah perlakuan dengan konsentrasi 80%, tingkat kematian mencapai 33,3% dan semakin meningkat seiring lamanya waktu aplikasi. Konsentrasi infusa daun cengkeh 20% efektif terhadap kematian larva nyamuk Ae. aegypti pada 3 jam pertama aplikasi, dan mencapai kematian larva 85% pada aplikasi selama 15 jam. Cengkeh varietas Afo berpotensi sebagai larvasida alami untuk penanggulangan vektor nyamuk malaria dan DBD.

Research paper thumbnail of Case method to increase student participation and problem-solving skills in biochemistry subject lesson study integrated at Khairun University

Bio-Inoved : Jurnal Biologi-Inovasi Pendidikan, Feb 16, 2023

The Biochemistry course is a basic and compulsory subject for Biology Education students. This st... more The Biochemistry course is a basic and compulsory subject for Biology Education students. This study aims to determine students' level of participation and problem-solving skills through the ability to solve cases of Biomolecules and proteins on Student Worksheets (LKM). This is an Action Research integrated with lesson study with two cycles. The instruments used were student participation rubrics in the case method and LKM problemsolving skills in the Biochemistry course. The subjects in this study were 24 third-semester students of the Biology Education Study Program. Data analysis techniques through descriptive statistics with percentages. The results showed an increase in the percentage from cycle 1 to cycle 2. Student participation in collaborative activities increased from 71.5% to 73.3%, while problem-solving skills increased from 53.5% to 71.7%. Based on the observer transcripts, some students still do not understand the material and cases discussed in lectures in class and the laboratory. This is because students do not actively ask questions and do not have curiosity about the material and case explanations presented by lecturers or fellow students. This study shows that implementing the integrated case method in lesson study can increase student participation activities and problem-solving skills in Biochemistry courses.

Research paper thumbnail of Bioassay and efficacy of clove flower stalk extract of Afo variety as a natural larvicidal

International Journal of Biological and Pharmaceutical Sciences Archive, Mar 30, 2023

This study aims to test the larvicidal bioassay of clove stalk extract against Aedes aegypti on a... more This study aims to test the larvicidal bioassay of clove stalk extract against Aedes aegypti on a laboratory scale and field scale efficacy test in a bath. Samples of sun-dried clove flower stalks were made into water extracts and coarse powder measuring 8-10 mesh for efficacy tests in the bath. The experimental method with a completely randomized design model was used to test the effectiveness of the larvicide, which consisted of two stages: The aqueous extract was tested against Ae. aegypti third instar larvae for three concentrations, namely 10%, 20%, and 30%, plus control treatment with three repetitions; observations were made at 30 minutes and 60 minutes after application to see the number of dead larvae as stage one. The second stage was the efficacy test in the bath which was carried out by inserting clove coarse powder in packs of 20 grams, 30 grams, and 40 grams, as well as the control treatment with five replicates, observed every day to see the time of emergence of mosquito larvae in a matter of days. The results of the first stage test showed an effect of the concentration of clove flower stalk extract on the mortality rate of the larvae, which was indicated by a positive relationship, where the mortality rate of the larvae reached 100% at 60 minutes after application. In comparison, the second stage test showed that there was an effect of clove flower stalk coarse powder on the time of larvae emergence, where the average time for larvae emergence was up to 8.8 days in the treatment of 40 g of clove flower stalk coarse powder, 1.83 times longer than the control (4 .8 days). Clove flower stalk extract is effective as a larvicidal mosquito Ae. aegypti.

Research paper thumbnail of Pengaruh Model Pembelajaran Talking Stick Terintegrasi Saintifik Berbantuan Media Charta Terhadap Hasil Belajar Kognitif Peserta Didik Kelas VIII SMP Negeri 16 Halmahera Selatan

JURNAL BIOEDUKASI

The scientifically integrated talking stick learning model assisted by charta media is a learning... more The scientifically integrated talking stick learning model assisted by charta media is a learning stage following the steps of the talking stick learning model with charta-assisted media to improve the ability of students' cognitive learning outcomes. This study aims to determine the effect of the scientifically integrated talking stick learning model assisted by chart media on students' cognitive learning outcomes. This research is a quasi-experimental research (Quasy Experiment). The sample of this study consisted of two classes, namely class VIII-1 as the experimental class and VIII-2 as the control class with a total of 35 students. The instrument used is a test to measure cognitive learning outcomes. Data analysis used the SPSS-assisted normality and homogeneity test, while the hypothesis test used the Anacova test. The results showed that the talking stick learning model with chart media had an effect on learning outcomes with a significant level of 0.05 0.001.

Research paper thumbnail of Case method to increase student participation and problem-solving skills in biochemistry subject lesson study integrated at Khairun University

BIO-INOVED : Jurnal Biologi-Inovasi Pendidikan

The Biochemistry course is a basic and compulsory subject for Biology Education students. This st... more The Biochemistry course is a basic and compulsory subject for Biology Education students. This study aims to determine students' level of participation and problem-solving skills through the ability to solve cases of Biomolecules and proteins on Student Worksheets (LKM). This is an Action Research integrated with lesson study with two cycles. The instruments used were student participation rubrics in the case method and LKM problem-solving skills in the Biochemistry course. The subjects in this study were 24 third-semester students of the Biology Education Study Program. Data analysis techniques through descriptive statistics with percentages. The results showed an increase in the percentage from cycle 1 to cycle 2. Student participation in collaborative activities increased from 71.5% to 73.3%, while problem-solving skills increased from 53.5% to 71.7%. Based on the observer transcripts, some students still do not understand the material and cases discussed in lectures in class...

Research paper thumbnail of Bioassay and efficacy of clove flower stalk extract of Afo variety as a natural larvicidal

International Journal of Biological and Pharmaceutical Sciences Archive

This study aims to test the larvicidal bioassay of clove stalk extract against Aedes aegypti on a... more This study aims to test the larvicidal bioassay of clove stalk extract against Aedes aegypti on a laboratory scale and field scale efficacy test in a bath. Samples of sun-dried clove flower stalks were made into water extracts and coarse powder measuring 8-10 mesh for efficacy tests in the bath. The experimental method with a completely randomized design model was used to test the effectiveness of the larvicide, which consisted of two stages: The aqueous extract was tested against Ae. aegypti third instar larvae for three concentrations, namely 10%, 20%, and 30%, plus control treatment with three repetitions; observations were made at 30 minutes and 60 minutes after application to see the number of dead larvae as stage one. The second stage was the efficacy test in the bath which was carried out by inserting clove coarse powder in packs of 20 grams, 30 grams, and 40 grams, as well as the control treatment with five replicates, observed every day to see the time of emergence of mosqu...

Research paper thumbnail of Pengembangan Perangkat Pembelajaran Biologi Berbasis Inkuiri Terbimbing Terintegrasi Saintifik Serta Pengaruhnya Terhadap Hasil Belajar Kognitif Dan Keterampilan Proses Sains Pada Siswa Kelas XI Sma Negeri 3 Halmahera Selatan

EDUKASI

The purpose of this study was (1) to determine whether the scientifically integrated guided inqui... more The purpose of this study was (1) to determine whether the scientifically integrated guided inquiry-based Biology learning device that was developed could be declared feasible to be used in class XI biology learning at SMA Negeri 3 Halmahera Selatan (2) to determine the effect of the scientifically integrated Guided Inquiry learning model on learning outcomes. cognitive skills in class XI SMA Negeri 3 South Halmahera (3) to determine the effect of the scientifically integrated Guided Inquiry learning model on science process skills in class XI students at SMA Negeri 3 Halmahera Selatan. This research is a research development or Research and Development (R&D). The sample of this study consisted of two classes, namely XI IPA-1 as an experimental class and Class XI IPA-2 as a control class with 36 students. The instrument used was an essay test for science process skills and for cognitive learning outcomes in the form of a multiple choice test (PG). ). Analysis of the data using data ...

Research paper thumbnail of Potensi cengkeh (Syzygium aromaticum) varietas Afo sebagai larvasida alami nyamuk Anopheles subpictus dan Aedes aegypti Potention of clove (Syzygium aromaticum) Afo variety as natural mosquito larvicidal of Anopheles subpictus and Aedes aegypti

Mer & Perry) varietas afo dikenal sebagai cengkeh tertua di dunia karena mencapai usia ratusan ta... more Mer & Perry) varietas afo dikenal sebagai cengkeh tertua di dunia karena mencapai usia ratusan tahun dan tumbuh di kawasan pegunungan Gamalama Ternate Maluku Utara. Kandungan minyak atsiri daun cengkeh afo sebesar 3.20% dan kadar eugenol 90.53% dapat dimanfaatkan sebagai larvasida alami. Pengendalian vektor nyamuk Anopheles sp. dan Aedes aegypti adalah salah satu langkah pengendalian penting untuk menanggulangi bahaya penyakit malaria dan (DBD) di Indonesia. Penelitian ini bertujuan untuk mengetahui efektivitas daun cengkeh varietas afo terhadap kematian larva nyamuk Anopheles subpictus dan Ae. aegypti. Metode penelitian adalah eksperimen dengan Rancangan Acak Lengkap (RAL). Larva nyamuk An. subpictus instar III sejumlah 330 ekor dibagi menjadi 10 perlakuan ekstrak air daun cengkeh (10%, 20%, 30%, 40%, 50%, 60%, 70%, 80%, 90% dan 100%) ditambah 1 kontrol dengan 3 kali ulangan. Setiap perlakuan berisi 10 ekor larva dan diamati setiap selang waktu 5 menit selama 30 menit. Pengujian larva Ae. aegypti instar III menggunakan ekstrak air dalam bentuk infusa daun cengkeh. Jumlah larva yang digunakan sebanyak 400 ekor dengan 4 perlakuan (5%, 10%, 15% dan 20%) ditambah 1 kontrol. Masing-masing kelompok perlakuan berisi 20 ekor larva dan dilakukan 4 kali ulangan. Pengamatan dilakukan setiap selang waktu 3 jam selama 15 jam dan dihitung jumlah larva yang mati. Hasil penelitian menunjukan bahwa efektivitas ekstrak daun cengkeh var. Afo terhadap larva An. subpictus terjadi pada menit ke 10 setelah perlakuan dengan konsentrasi 80%, tingkat kematian mencapai 33,3% dan semakin meningkat seiring lamanya waktu aplikasi. Konsentrasi infusa daun cengkeh 20% efektif terhadap kematian larva nyamuk Ae. aegypti pada 3 jam pertama aplikasi, dan mencapai kematian larva 85% pada aplikasi selama 15 jam. Cengkeh varietas Afo berpotensi sebagai larvasida alami untuk penanggulangan vektor nyamuk malaria dan DBD.

Research paper thumbnail of Pengujian Antimalaria Menggunakan Tanaman Cengkeh (Syzygium aromaticum (L.) Merr & Perry) (Studi in vitro, in vivo, Toksisitas dan Screening Senyawa Aktif)

Research paper thumbnail of ANTIMALARIAL ACTIVITY OF METHANOL EXTRACT OF CLOVE FLOWER AND CLOVE STALK FLOWER OF TUNI BURU SELATAN VARIETIES ON Plasmodium berghei

The methanol extract of clove flower and clove flower stalk varieties of tuni Buru selatan were e... more The methanol extract of clove flower and clove flower stalk varieties of tuni Buru selatan were evaluated for in vivo antimalarial activity, in a 4-day suppression test against Plasmodium berghei in mice. The in vivo model for testing the antimalarial effects of plant extracts is described. Selected mice (20-30 g body weight) were divided into 4 groups, each consisting of 5 mice. Each animal is injected with red blood cells Plasmodium berghei. Testing was done by Peter test, mice were given oral treatment with diluted content 25 mg / kg bw, 50 mg / kg bw and 100 mg / kg bw for 4 groups. One group served as negative control. Negative controls were given 0.5% CMC Na solution. ED50 extract methanol of clove flower and clove stalk flower varieties of Tuni Buru selatan in Plasmodium berghei mice were 39,96 and 20,023 mg / kg bw. Keywords: antimalarial, clove flower, clove flower stalk, Plasmodium berghei

Research paper thumbnail of Pendampingan pembuatan hand sanitizer berbasis kearifan lokal (ekstrak tangkai bunga cengkeh)

ABSYARA: Jurnal Pengabdian Pada Masyarakat, 2020

Efforts to prevent viral infection are a shared responsibility of all Indonesian citizens, includ... more Efforts to prevent viral infection are a shared responsibility of all Indonesian citizens, including academics. Prevention starts from keeping a distance, wearing a mask, and washing hands. Another alternative to washing hands is to use a hand sanitizer. This community service activity program aims to educate and assist the community in preventing infection with the Covid-19 virus based on local culture through assisting students in making hand sanitizers made from local products, namely clove flower stem extract, and distributing them to the community. The methods used in this activity are production, product distribution, and education (lectures). The team will distribute the product to the Khairun University campus area and six schools in Ternate city. This community service program's result is 300 bottles of clove flower stalk extract and distributed to the public. In general, the community's response to the hand sanitizer product from clove flower stalk extract is that ...

Research paper thumbnail of Ekstrak Metanol Cengkeh (Syzygium Aromaticum (L.) Merry & Perry) Varietas Tuni Buru Selatan sebagai Antimalaria

Acta VETERINARIA Indonesiana, 2018

Malaria masih menjadi salah satu masalah kesehatan di dunia dan Indonesia. Cengkeh (Syzygium arom... more Malaria masih menjadi salah satu masalah kesehatan di dunia dan Indonesia. Cengkeh (Syzygium aromaticum (L.) Merry…

Research paper thumbnail of Pengetahuan Lokal Masyarakat Kepulauan Tentang Tumbuhan Anti-nyamuk

Techno: Jurnal Penelitian, 2020

Pemanfaatan bahan alami dari tanaman lokal sebagai anti-nyamuk dimulai dari pendalaman informasi ... more Pemanfaatan bahan alami dari tanaman lokal sebagai anti-nyamuk dimulai dari pendalaman informasi tentang pengetahuan lokal masyarakat. Upaya ini perlu dilakukan untuk mengatasi beberapa permasalahan dari penggunaan anti-nyamuk sintetik yang beredar di pasaran, juga sebagai bagian dari upaya melestarikan kearifan lokal masyarakat yang mulai tergerus oleh kemajuan zaman. Tujuan penelitian ini adalah untuk mendeskripsikan pengetahuan lokal masyarakat kepulauan tentang pemanfaatan tumbuhan anti-nyamuk dan menganalisis potensi tumbuhan yang paling banyak digunakan masyarakat di wilayah kepulauan sebagai produk lotion anti-nyamuk. Metode yang digunakan adalah metode survei eksploratif. Pengumpulan data dilakukan dengan teknik wawancara, observasi secara mendalam terhadap informan dan dokumentasi. Data jenis tumbuhan anti-nyamuk yang paling banyak digunakan dianalisis komponen fitokimia. Hasil penelitian menunjukkan terdapat 9 jenis tumbuhan anti-nyamuk yang digunakan masyarakat yaitu Tagetes erecta L., Lavandula L., Cymbopogon citratus, Citrus aurantifolia, Syzygium aromaticum, Evodia suaveolens, Catharanthus roseus (L) G. Don., Alphitonia incana BL. dan Polygala paniculata. Jenis Alphitonia incana BL paling banyak digunakan masyarakat dan mengandung senyawa fitokimia alkaloid, terpenoid, flavonoid, fenolik dan tanin. Jenis Alphitonia incana BL berpotensi untuk dikembangkan sebagai produk lotion anti-nyamuk.

Research paper thumbnail of Serological and molecular prevalence of equine piroplasmosis in Western Java, Indonesia

Veterinary Parasitology: Regional Studies and Reports, 2018

Equine piroplasmosis is an economically significant disease caused by Theileria equi and Babesia ... more Equine piroplasmosis is an economically significant disease caused by Theileria equi and Babesia caballi, which are tick-borne hemoprotozoan parasites. Infections with these parasite species had never been reported in horses in Indonesia. The aim of the present study was to investigate the prevalence of T. equi and B. caballi in horses reared in parts of Western Java, Indonesia. Blood samples were collected randomly from 235 horses in four different districts (Bandung, Depok, Tangerang, and Bogor) in Western Java, Indonesia. Thin blood smears prepared from the sampled animals were stained by Giemsa and observed under a light microscope. Serum samples prepared from blood were screened by enzyme-linked immunosorbent assays (ELISAs) based on recombinant forms of EMA-2 and BC48 antigens to determine the seroprevalence of T. equi and B. caballi, respectively. DNA samples extracted from the same blood samples were screened by EMA-2 and BC48 gene-based nested polymerase chain reaction (nPCR) assays for T. equi and B. caballi infections, respectively. Of 235 surveyed animals, five (2.1%) and 15 (6.4%) were seropositive for T. equi and B. caballi, respectively, whereas one and four horses were nPCR-positive for T. equi and B. caballi, respectively. All of the surveyed animals were negative for T. equi and B. caballi by microscopy. The T. equi EMA-2 and B. caballi BC48 gene fragments amplified by the nPCR assays were cloned, sequenced, and subjected to bioinformatic and phylogenetic analyses. The T. equi EMA-2 gene sequence from an Indonesian horse was identical to sequences from Florida and Washington strains and clustered together with these sequences in phylogeny. On the other hand, four Indonesian BC48 gene sequences shared 99.8-100% identity scores. This present study is the first to report T. equi and B. caballi in horses in Indonesia. Our findings highlight the need for monitoring horses in Indonesia for clinical piroplasmosis caused by T. equi and B. caballi.