Iulia Lupan | Babes-Bolyai University (original) (raw)
Papers by Iulia Lupan
Children
The three types of neurofibromatosis, namely type 1, type 2, and schwannomatosis, are generally a... more The three types of neurofibromatosis, namely type 1, type 2, and schwannomatosis, are generally associated with various benign tumors affecting the skin and the nervous system. On rare occasions, especially in patients with neurofibromatosis type 1 (NF1), malignant neoplasms may also be present, several of them possessing a more aggressive course than in individuals without this syndrome. As such, a clear delineation between the three variants of neurofibromatosis is crucial to establish the correct diagnosis and management, as well as predict the neoplasm-related outcomes. Neurofibromin, the principal product of the NF1 gene, is a potent inhibitor of cellular proliferation, having been linked to several key signaling pathways involved in tumor growth. Therefore, it may provide a useful therapeutic target for tumor management in these patients. In this article, we want to present the association between deficiency of neurofibromin and the consequences of the lack of this protein lea...
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The Protein Journal
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Allergologia et Immunopathologia
Atopic dermatitis (AD) is mainly considered an allergy, exacerbated by allergic factors. Is there... more Atopic dermatitis (AD) is mainly considered an allergy, exacerbated by allergic factors. Is there evidence to suggest the existence of autoimmune components in the pathophysiology of the illness? Studies in the literature that dealt with the occurrence of autoimmunity in children with AD were analyzed. We followed the studies published in PubMed for 10 years, from 2001 to 2021. Clinical signs and symptoms were similar to other autoimmune diseases, having periods of remission and relapses. Other correlations between AD and autoimmune diseases have been described, and patients with AD can also present with a wide range of autoimmune comorbidities. Three major factors contribute to the pathogenesis of AD: damage of the skin barrier, disorders of the immune response, and imbalances of the skin microbiome—all based on genetic changes and influenced by environmental factors. Predominant activation of Th 2 cells, with the increase of Th 1, Th 17, and Th 22 subsets, promotes skin inflammati...
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Research Square (Research Square), Sep 8, 2022
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International Journal of Celiac Disease, 2016
Introduction. Celiac disease has a prevalence of 1% of the global population and it is increasing... more Introduction. Celiac disease has a prevalence of 1% of the global population and it is increasing every year. Viral infections such as hepatitis C and hepatitis B are the most common liver diseases and studies show that may be a link between them and celiac disease. Recent studies show attempts to find a link between viral infections and celiac disease. We focused our study on the association between viral infections and celiac disease. Material and Method. We studied 63 articles from the Pub Med Database that revealed a possible connection between viral infections and celiac disease. Results. Since it is more frequent, hepatitis C is associated with the development of celiac disease, but celiac disease is not the autoimmune manifestation of hepatitis C. Patients with celiac disease have a significantly decreased response to hepatitis B vaccine. Other viruses that are associated with celiac disease are: rotavirus, adenovirus, eterovirus and Epstein-Barr virus. Conclusions. The serol...
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International Journal of Celiac Disease, 2016
Celiac disease percentage is continuously increasing in recent years. Helicobacter pylori infecti... more Celiac disease percentage is continuously increasing in recent years. Helicobacter pylori infection is found in more than 50% of the population. Recent studies have attempted to discover a link between celiac disease and H. pylori infection. We analyzed the main immunopathological aspects of celiac disease associated with H. pylori infection. If celiac disease is associated with H. pylori infection, clinical evidence can occur: gastric lymphocytosis, duodenal lymphocytosis.
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Scientific Reports, 2020
An amendment to this paper has been published and can be accessed via a link at the top of the pa... more An amendment to this paper has been published and can be accessed via a link at the top of the paper.
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Romanian Journal of Neurology, 2021
The pathology of Dravet syndrome is of particular importance in children. In this article we anal... more The pathology of Dravet syndrome is of particular importance in children. In this article we analyze the involvement of the SCN1A gene in Dravet syndrome, we approach the new treatment strategies in Dravet syndrome.
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International Journal of Celiac Disease, 2016
The celiac disease screening with IgA tissue transglutaminase antibodies depends on the cut-off r... more The celiac disease screening with IgA tissue transglutaminase antibodies depends on the cut-off reaction, therefore many patients with values less but close to the cut-off may be undiagnosed. Material and Method. We conducted the CD screening with IgA tissue transglutaminase antibodies in a group of 1616 children during 2014 at a cut-off of 25 U/ml and at a cut-off of 10 U/ml. We also wanted to compare our results with IgA tissue transglutaminase antibodies prevalence from other countries. Results. We found a IgA tissue transglutaminase antibodies prevalence rate of 3% for values higher 25 U/ml and a IgA tissue transglutaminase antibodies prevalence rate of 1.6% for values between 10-25 U/ml. A prevalence of 13.6% for IgA tissue transglutaminase antibodies was observed in , and . Conclusions. We observed a 4.6% IgA tissue transglutaminase antibodies prevalence at a cut-off of 10 U/ml. Prevalence of IgA tissue transglutaminase antibodies from Romania is lowered compared with other Eu...
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International Journal of Celiac Disease, 2017
In the accordance of readers, we are introducing a new chapter in Journal, which will consist in ... more In the accordance of readers, we are introducing a new chapter in Journal, which will consist in Clinical Scenarios for Medical Students. First clinical scenario is presented by associate professor Sur Genel and his team: a 12-year-old girl diagnosed with a combination of four autoimmune diseases: autoimmune hepatitis, thyroiditis, celiac disease and type 1 diabetes mellitus.
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Journal of Clinical Medicine, 2020
Objectives. The aim of this study is to provide information about prevalence, etiology, risk fact... more Objectives. The aim of this study is to provide information about prevalence, etiology, risk factors, clinical characteristics and endoscopic features of various types of infectious esophagitis in children. Methods. We performed a total of 520 upper gastrointestinal tract endoscopies in Pediatric Clinic II, Emergency Hospital for Children, Cluj-Napoca. Indications for endoscopy in our cohort were gastrointestinal tract symptoms such as dysphagia, heartburn, or appetite loss. Results. The prevalence of infectious esophagitis in the study population was 2.11% (11 patients). Candida albicans (C. albicans) was the most frequent cause. Our data illustrates that herpes simplex virus (HSV)-induced esophagitis is common in immunocompromised patients and should be systematically suspected in cases of severe dysphagia, heartburn, or hematemesis. In the present study, all cytomegalovirus (CMV) esophagitis patients were immunocompromised. Immunodeficiency (81.8%) and prolonged antibiotic therap...
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Expert Review of Proteomics, 2019
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Journal of Laboratory Medicine, 2019
Background This study aimed to investigate the implications of antinuclear antibodies (ANAs) in j... more Background This study aimed to investigate the implications of antinuclear antibodies (ANAs) in juvenile idiopathic arthritis (JIA), the association with uveitis and the favorable evolution of the disease. Methods We followed 45 pediatric patients suffering from JIA, according to the International League of Associations for Rheumatology (ILAR), for a period of 2 years. ANAs were followed through immunofluorescence methods using a high-performance immunofluorescence automatic Helios device. Results A total of 15 of the ANA-positive patients presented an oligoarticular form of the disease, eight of them presented a polyarticular form of the disease and two of them presented a systemic form. The most severe forms have associated iridocyclitis. ANA-positive patients presented an earlier onset of disease in comparison with ANA-negative ones. ANA-positive patients from different ILAR categories had similar characteristics of the disease such as onset age (earlier), higher prevalence in fe...
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Central European Journal of Immunology, 2019
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Clinical laboratory, 2018
The detection of sideroblastic anemia in a newborn may suggest developing Pearson syndrome. The p... more The detection of sideroblastic anemia in a newborn may suggest developing Pearson syndrome. The prognosis of these patients is severe and death occurs in the first 3 years of life, so it is important to find new ways of diagnosis. Case Presentation: In the case of our patient the diagnosis was supported only at the age of 5 months, highlighting the difficulties of diagnosis at this age. The diagnosis of Pearson syndrome with neonatal onset is difficult to sustain or even impossible at that age. This diagnosis can be confirmed and supported during disease progression.
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International Journal of Celiac Disease, 2016
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International Journal of Celiac Disease, 2014
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Scientific Reports, 2020
A mud volcano (MV) is a naturally hydrocarbon-spiked environment, as indicated by the presence of... more A mud volcano (MV) is a naturally hydrocarbon-spiked environment, as indicated by the presence of various quantities of PAHs and aromatic isotopic shifts in its sediments. Recurrent expulsion of various hydrocarbons consolidates the growth of hydrocarbonoclastic bacterial communities in the areas around MVs. In addition to the widely-known availability of biologically malleable alkanes, MVs can represent hotbeds of polyaromatic hydrocarbons (PAHs), as well - an aspect that has not been previously explored. This study measured the availability of highly recalcitrant PAHs and the isotopic signature of MV sediments both by GC-MS and δ13C analyses. Subsequently, this study highlighted both the occurrence and distribution of putative PAH-degrading bacterial OTUs using a metabarcoding technique. The putative hydrocarbonoclastic taxa incidence are the following: Enterobacteriaceae (31.5%), Methylobacteriaceae (19.9%), Bradyrhizobiaceae (16.9%), Oxalobacteraceae (10.2%), Comamonadaceae (7.6...
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Life
Viral infections are a key issue in modern medicine. SARS-CoV-2 infection confirms that we are no... more Viral infections are a key issue in modern medicine. SARS-CoV-2 infection confirms that we are not sufficiently prepared for these unforeseen infections. The COVID-19 pandemic has cultivated a great sense of fear and distrust in patients. If viral infections, in this case, SARS-CoV-2, overlap with another infection, the symptoms are prolonged and worsened, and complications may occur. Starting from an objective clinical finding of a patient they had in follow-up and treatment, the authors present the problems of the diseases the patient suffered from. These are described as reviews so that readers can get an idea of the clinical methods of expression and the therapeutic possibilities. Therefore, this article describes Lyme disease and post-treatment Lyme disease syndrome, SARS-CoV-2 infection, and multisystem inflammatory syndrome in children (MISC-C), as the patient suffered from an incomplete form of Kawasaki disease. During the treatment for Lyme disease, the patient also contrac...
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Gastroenterology and Hepatology From Bed to Bench, 2017
Celiac disease (CD) was initially a real enigma. There were many questions about what immunogenet... more Celiac disease (CD) was initially a real enigma. There were many questions about what immunogenetics markers could influence the immune response (1). Immunogenetic studies provided important contributions to improve the understanding of critical pathogenetic factors at molecular levels (DQ2, DQ8) (2). An important discovery was the recognition of more precise serological markers for CD, specifically anti-endomysial and anti-tissue transglutaminase antibodies leading to an exploration of their increasingly important role in clinical diagnosis of CD (3). Our aim is to explore new and old challenges with unanswered questions in CD diagnosis. The last Prague consensus report challenges Re-evaluation of CD diagnostic with intestinal biopsy is required in this consesus (4). However, many patients with CD are still inaccurately diagnosed (or remain undiagnosed), even in referral populations whom were evaluated at tertiary care centers: the diagnosis of CD was confirmed in only 64 patients ...
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Children
The three types of neurofibromatosis, namely type 1, type 2, and schwannomatosis, are generally a... more The three types of neurofibromatosis, namely type 1, type 2, and schwannomatosis, are generally associated with various benign tumors affecting the skin and the nervous system. On rare occasions, especially in patients with neurofibromatosis type 1 (NF1), malignant neoplasms may also be present, several of them possessing a more aggressive course than in individuals without this syndrome. As such, a clear delineation between the three variants of neurofibromatosis is crucial to establish the correct diagnosis and management, as well as predict the neoplasm-related outcomes. Neurofibromin, the principal product of the NF1 gene, is a potent inhibitor of cellular proliferation, having been linked to several key signaling pathways involved in tumor growth. Therefore, it may provide a useful therapeutic target for tumor management in these patients. In this article, we want to present the association between deficiency of neurofibromin and the consequences of the lack of this protein lea...
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The Protein Journal
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Allergologia et Immunopathologia
Atopic dermatitis (AD) is mainly considered an allergy, exacerbated by allergic factors. Is there... more Atopic dermatitis (AD) is mainly considered an allergy, exacerbated by allergic factors. Is there evidence to suggest the existence of autoimmune components in the pathophysiology of the illness? Studies in the literature that dealt with the occurrence of autoimmunity in children with AD were analyzed. We followed the studies published in PubMed for 10 years, from 2001 to 2021. Clinical signs and symptoms were similar to other autoimmune diseases, having periods of remission and relapses. Other correlations between AD and autoimmune diseases have been described, and patients with AD can also present with a wide range of autoimmune comorbidities. Three major factors contribute to the pathogenesis of AD: damage of the skin barrier, disorders of the immune response, and imbalances of the skin microbiome—all based on genetic changes and influenced by environmental factors. Predominant activation of Th 2 cells, with the increase of Th 1, Th 17, and Th 22 subsets, promotes skin inflammati...
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Research Square (Research Square), Sep 8, 2022
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International Journal of Celiac Disease, 2016
Introduction. Celiac disease has a prevalence of 1% of the global population and it is increasing... more Introduction. Celiac disease has a prevalence of 1% of the global population and it is increasing every year. Viral infections such as hepatitis C and hepatitis B are the most common liver diseases and studies show that may be a link between them and celiac disease. Recent studies show attempts to find a link between viral infections and celiac disease. We focused our study on the association between viral infections and celiac disease. Material and Method. We studied 63 articles from the Pub Med Database that revealed a possible connection between viral infections and celiac disease. Results. Since it is more frequent, hepatitis C is associated with the development of celiac disease, but celiac disease is not the autoimmune manifestation of hepatitis C. Patients with celiac disease have a significantly decreased response to hepatitis B vaccine. Other viruses that are associated with celiac disease are: rotavirus, adenovirus, eterovirus and Epstein-Barr virus. Conclusions. The serol...
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International Journal of Celiac Disease, 2016
Celiac disease percentage is continuously increasing in recent years. Helicobacter pylori infecti... more Celiac disease percentage is continuously increasing in recent years. Helicobacter pylori infection is found in more than 50% of the population. Recent studies have attempted to discover a link between celiac disease and H. pylori infection. We analyzed the main immunopathological aspects of celiac disease associated with H. pylori infection. If celiac disease is associated with H. pylori infection, clinical evidence can occur: gastric lymphocytosis, duodenal lymphocytosis.
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Scientific Reports, 2020
An amendment to this paper has been published and can be accessed via a link at the top of the pa... more An amendment to this paper has been published and can be accessed via a link at the top of the paper.
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Romanian Journal of Neurology, 2021
The pathology of Dravet syndrome is of particular importance in children. In this article we anal... more The pathology of Dravet syndrome is of particular importance in children. In this article we analyze the involvement of the SCN1A gene in Dravet syndrome, we approach the new treatment strategies in Dravet syndrome.
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International Journal of Celiac Disease, 2016
The celiac disease screening with IgA tissue transglutaminase antibodies depends on the cut-off r... more The celiac disease screening with IgA tissue transglutaminase antibodies depends on the cut-off reaction, therefore many patients with values less but close to the cut-off may be undiagnosed. Material and Method. We conducted the CD screening with IgA tissue transglutaminase antibodies in a group of 1616 children during 2014 at a cut-off of 25 U/ml and at a cut-off of 10 U/ml. We also wanted to compare our results with IgA tissue transglutaminase antibodies prevalence from other countries. Results. We found a IgA tissue transglutaminase antibodies prevalence rate of 3% for values higher 25 U/ml and a IgA tissue transglutaminase antibodies prevalence rate of 1.6% for values between 10-25 U/ml. A prevalence of 13.6% for IgA tissue transglutaminase antibodies was observed in , and . Conclusions. We observed a 4.6% IgA tissue transglutaminase antibodies prevalence at a cut-off of 10 U/ml. Prevalence of IgA tissue transglutaminase antibodies from Romania is lowered compared with other Eu...
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International Journal of Celiac Disease, 2017
In the accordance of readers, we are introducing a new chapter in Journal, which will consist in ... more In the accordance of readers, we are introducing a new chapter in Journal, which will consist in Clinical Scenarios for Medical Students. First clinical scenario is presented by associate professor Sur Genel and his team: a 12-year-old girl diagnosed with a combination of four autoimmune diseases: autoimmune hepatitis, thyroiditis, celiac disease and type 1 diabetes mellitus.
Bookmarks Related papers MentionsView impact
Journal of Clinical Medicine, 2020
Objectives. The aim of this study is to provide information about prevalence, etiology, risk fact... more Objectives. The aim of this study is to provide information about prevalence, etiology, risk factors, clinical characteristics and endoscopic features of various types of infectious esophagitis in children. Methods. We performed a total of 520 upper gastrointestinal tract endoscopies in Pediatric Clinic II, Emergency Hospital for Children, Cluj-Napoca. Indications for endoscopy in our cohort were gastrointestinal tract symptoms such as dysphagia, heartburn, or appetite loss. Results. The prevalence of infectious esophagitis in the study population was 2.11% (11 patients). Candida albicans (C. albicans) was the most frequent cause. Our data illustrates that herpes simplex virus (HSV)-induced esophagitis is common in immunocompromised patients and should be systematically suspected in cases of severe dysphagia, heartburn, or hematemesis. In the present study, all cytomegalovirus (CMV) esophagitis patients were immunocompromised. Immunodeficiency (81.8%) and prolonged antibiotic therap...
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Expert Review of Proteomics, 2019
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Journal of Laboratory Medicine, 2019
Background This study aimed to investigate the implications of antinuclear antibodies (ANAs) in j... more Background This study aimed to investigate the implications of antinuclear antibodies (ANAs) in juvenile idiopathic arthritis (JIA), the association with uveitis and the favorable evolution of the disease. Methods We followed 45 pediatric patients suffering from JIA, according to the International League of Associations for Rheumatology (ILAR), for a period of 2 years. ANAs were followed through immunofluorescence methods using a high-performance immunofluorescence automatic Helios device. Results A total of 15 of the ANA-positive patients presented an oligoarticular form of the disease, eight of them presented a polyarticular form of the disease and two of them presented a systemic form. The most severe forms have associated iridocyclitis. ANA-positive patients presented an earlier onset of disease in comparison with ANA-negative ones. ANA-positive patients from different ILAR categories had similar characteristics of the disease such as onset age (earlier), higher prevalence in fe...
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Central European Journal of Immunology, 2019
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Clinical laboratory, 2018
The detection of sideroblastic anemia in a newborn may suggest developing Pearson syndrome. The p... more The detection of sideroblastic anemia in a newborn may suggest developing Pearson syndrome. The prognosis of these patients is severe and death occurs in the first 3 years of life, so it is important to find new ways of diagnosis. Case Presentation: In the case of our patient the diagnosis was supported only at the age of 5 months, highlighting the difficulties of diagnosis at this age. The diagnosis of Pearson syndrome with neonatal onset is difficult to sustain or even impossible at that age. This diagnosis can be confirmed and supported during disease progression.
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International Journal of Celiac Disease, 2016
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International Journal of Celiac Disease, 2014
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Scientific Reports, 2020
A mud volcano (MV) is a naturally hydrocarbon-spiked environment, as indicated by the presence of... more A mud volcano (MV) is a naturally hydrocarbon-spiked environment, as indicated by the presence of various quantities of PAHs and aromatic isotopic shifts in its sediments. Recurrent expulsion of various hydrocarbons consolidates the growth of hydrocarbonoclastic bacterial communities in the areas around MVs. In addition to the widely-known availability of biologically malleable alkanes, MVs can represent hotbeds of polyaromatic hydrocarbons (PAHs), as well - an aspect that has not been previously explored. This study measured the availability of highly recalcitrant PAHs and the isotopic signature of MV sediments both by GC-MS and δ13C analyses. Subsequently, this study highlighted both the occurrence and distribution of putative PAH-degrading bacterial OTUs using a metabarcoding technique. The putative hydrocarbonoclastic taxa incidence are the following: Enterobacteriaceae (31.5%), Methylobacteriaceae (19.9%), Bradyrhizobiaceae (16.9%), Oxalobacteraceae (10.2%), Comamonadaceae (7.6...
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Life
Viral infections are a key issue in modern medicine. SARS-CoV-2 infection confirms that we are no... more Viral infections are a key issue in modern medicine. SARS-CoV-2 infection confirms that we are not sufficiently prepared for these unforeseen infections. The COVID-19 pandemic has cultivated a great sense of fear and distrust in patients. If viral infections, in this case, SARS-CoV-2, overlap with another infection, the symptoms are prolonged and worsened, and complications may occur. Starting from an objective clinical finding of a patient they had in follow-up and treatment, the authors present the problems of the diseases the patient suffered from. These are described as reviews so that readers can get an idea of the clinical methods of expression and the therapeutic possibilities. Therefore, this article describes Lyme disease and post-treatment Lyme disease syndrome, SARS-CoV-2 infection, and multisystem inflammatory syndrome in children (MISC-C), as the patient suffered from an incomplete form of Kawasaki disease. During the treatment for Lyme disease, the patient also contrac...
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Gastroenterology and Hepatology From Bed to Bench, 2017
Celiac disease (CD) was initially a real enigma. There were many questions about what immunogenet... more Celiac disease (CD) was initially a real enigma. There were many questions about what immunogenetics markers could influence the immune response (1). Immunogenetic studies provided important contributions to improve the understanding of critical pathogenetic factors at molecular levels (DQ2, DQ8) (2). An important discovery was the recognition of more precise serological markers for CD, specifically anti-endomysial and anti-tissue transglutaminase antibodies leading to an exploration of their increasingly important role in clinical diagnosis of CD (3). Our aim is to explore new and old challenges with unanswered questions in CD diagnosis. The last Prague consensus report challenges Re-evaluation of CD diagnostic with intestinal biopsy is required in this consesus (4). However, many patients with CD are still inaccurately diagnosed (or remain undiagnosed), even in referral populations whom were evaluated at tertiary care centers: the diagnosis of CD was confirmed in only 64 patients ...
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New treatments in celiac disease are being vigorously pursued to either replace or facilitate the... more New treatments in celiac disease are being vigorously pursued to either replace or facilitate the difficult-to-follow gluten-free diet. There are numerous difficulties in adhering to gluten withdrawal, but the adolescence and young adulthood is considered a fragile and high risk period for intentional and unintentional gluten intake. This is only one of the aspects that were reviewed and recommendations issued, in the Prague consensus report on the transition from childhood to adulthood in celiac disease patients. The present review intends to summarize the challenges in gluten-free diet adherence during the transitional period, as reflected in the last Prague concensus, published in 2016. The honorable panel members recommended that dietary adherence and the consequences of non-adherence represent key components for discussion in the transitional period setting.
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no
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With all the advancements of science, genetic tests are important for the diagnosis of celiac dis... more With all the advancements of science, genetic tests are important for the diagnosis of celiac disease but a lack of standardization is evidenced.
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Allergologia et Immunopathologia, 2017
Many recent studies discredit breastfeeding protection against coeliac disease. We will try to an... more Many recent studies discredit breastfeeding protection against coeliac disease. We will try to answer the question: “Is the evidence of breast feeding protection against coeliac disease real?”
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Celiac disease remains one of the most challenging pathologies of the small intestine. It involve... more Celiac disease remains one of the most challenging pathologies of the small intestine. It involves multiple pathogenic pathways and there are no disease-changing pharmacological agents available against it yet. The term microbiota refers to the community of microorganisms that inhabit a particular region of the body. Normal gut microbiota has a vital role in maintaining the intestinal homeostasis and promoting health. Celiac disease is associated with microbiota alteration, especially with an increase in the number of Gram-negative bacteria and a decrease in the number of Gram-positive bacteria. There is a strong relationship between intestinal dysbiosis and celiac disease, and recent studies are aimed at determining whether the celiac disease is a risk factor for dysbiosis or dysbiosis is for celiac disease. Therefore, the aim of this review was to assess the latest findings regarding the gut microbiota and its impact on the celiac disease, including therapeutic aspects.
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Introduction. Celiac disease has a prevalence of 1% of the global population and it is increasing... more Introduction. Celiac disease has a prevalence of 1% of the global population and it is increasing every year. Viral infections such as hepatitis C and hepatitis B are the most common liver diseases and studies show that may be a link between them and celiac disease. Recent studies show attempts to find a link between viral infections and celiac disease. We focused our study on the association between viral infections and celiac disease. Material and Method. We studied 63 articles from the Pub Med Database that revealed a possible connection between viral infections and celiac disease. Results. Since it is more frequent, hepatitis C is associated with the development of celiac disease, but celiac disease is not the autoimmune manifestation of hepatitis C. Patients with celiac disease have a significantly decreased response to hepatitis B vaccine. Other viruses that are associated with celiac disease are: rotavirus, adenovirus, eterovirus and Epstein-Barr virus. Conclusions. The serological screening for celiac disease at hepatitis C patients, after starting treatment with interferon, is necessary. Also, it is justified the serological screening for celiac disease among adults infected with hepatitis B. Further studies are still needed to show the possible association of viral infections in the pathogenesis of celiac disease.
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no abstract
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Celiac disease percentage is continuously increasing in recent years. Helicobacter pylori infecti... more Celiac disease percentage is continuously increasing in recent years. Helicobacter pylori infection is found in more than 50% of the population. Recent studies have attempted to discover a link between celiac disease and H. pylori infection. We analyzed the main immunopathological aspects of celiac disease associated with H. pylori infection. If celiac disease is associated with H. pylori infection, clinical evidence can occur: gastric lymphocytosis, duodenal lymphocytosis.
Bookmarks Related papers MentionsView impact
The celiac disease screening with IgA tissue transglutaminase antibodies depends on the cutoff re... more The celiac disease screening with IgA tissue transglutaminase antibodies depends on the cutoff reaction, therefore many patients with values less but close to the cutoff may be undiagnosed. Material and Method. We conducted the CD screening with IgA tissue transglutaminase antibodies in a group of 1616 children during 2014 at a cutoff of 25 U/ml and at a cutoff of 10 U/ml. We also wanted to compare our results with IgA tissue transglutaminase antibodies prevalence from other countries. Results. We found a IgA tissue transglutaminase antibodies prevalence rate of 3% for values higher 25 U/ml and a IgA tissue transglutaminase antibodies prevalence rate of 1.6% for values between 10-25 U/ml. A prevalence of 13.6% for IgA tissue transglutaminase antibodies was observed in Germany, Finland and Sweden. Conclusions. We observed a 4.6% IgA tissue transglutaminase antibodies prevalence at a cutoff of 10 U/ml. Prevalence of IgA tissue transglutaminase antibodies from Romania is lowered compared with other European countries.
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The " four out of five rule " has not been officially endorsed by official guidelines. However, H... more The " four out of five rule " has not been officially endorsed by official guidelines. However, HLA-associated genes that predispose to CD are found in as much as 30–40% of healthy people. Thus, their presence seems too indecisive for the occurrence of CD.
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Celiac disease is still under diagnosed, although many diagnostic tests are available. Serologica... more Celiac disease is still under diagnosed, although many diagnostic tests are available. Serological tests revealed many clinical aspects of celiac disease. We will discuss in this review the most important findings in terms of diagnosis of celiac disease.
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Central European Journal of Immunology, 2014
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no abstract
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Intestinal microbiota plays an important role in maintaining the overall health of an individual.... more Intestinal microbiota plays an important role in maintaining the overall health of an individual. It can be affected by diet but also inflammation of the intestine due to various causes. In the last decade, particular attention has been paid to the study of the interaction between mucosal cells and intestinal microbiota, and to the host immune response to change in community structure. Here, we review the most significant studies on human microbiota in patients with celiac disease, and also the potential biotechnological use of microorganisms for the production of gluten-free products.
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Many recent studies overshadow the effects of gluten-free diet. Gluten-free diet positive effects... more Many recent studies overshadow the effects of gluten-free diet. Gluten-free diet positive effects were observed in celiac disease patients: increase in body mass index, higher energy intakes, reducing adiposity gain, moderates the risk of the associated complications. However, adhering to a gluten-free diet is difficult for many people. A new solution is needed for quality of life of celiac disease patients, not for celiac disease treatment. Health education on gluten-free diet at home and in society seems to be the solution. The aim of our study is to evaluate the recent research on gluten-free diet as a nutritional therapy for patients with celiac disease. To achieve this purpose we have analyzed the published studies from 2008 to the present on nutrition in celiac disease.
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International Journal of Celiac Disease, 2014
Evolution of celiac disease is continuously changing. Celiac disease has become a public health p... more Evolution of celiac disease is continuously changing. Celiac disease has become a public health problem in some countries. Knowledge of the disease evolution is important to find current problems of disease. In this article, we wanted to discuss the recent medical and diagnostic discoveries from Romania.
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no abstract
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Celiac disease has become extensively studied. What could be the cause? Increasing the accuracy o... more Celiac disease has become extensively studied. What could be the cause? Increasing the accuracy of diagnostic tests for celiac disease has led to more discovered cases. Serological diagnosis of celiac disease has undergone important changes in recent years. Application of serological tests has reflected the diagnostic performance of tissue transglutaminase antibody and endomysial antibody as screening tests for celiac disease but also the progress of new serological tests as the antibodies against synthetic deamidated gliadin peptides. Serological tests are largely responsible for the recognition that celiac disease is not a rare disease. The Consensus in celiac disease from 2008 conducted under the aegis of the European Society of Pediatric Gastroenterology, Hepatology and Nutrition jointly with North American Society of Pediatric Gastroenterology, Hepatology and Nutrition which agreed that “Celiac disease is an immune-mediated enteropathy that can affect any system or organ and that can present itself with a wide range of clinical manifestations of variable severity” was confirmed. But increasing prevalence of this disease has led to the need for new methods of treatment among patients with celiac disease. Studies on quality of life of patients with celiac disease have questioned the gluten-free diet. As such new therapies, like TG2 inhibitors, the copolymer P (HEMA-co-SS) and other new experimental therapies, have emerged in celiac disease. The new therapies in celiac disease are based on new investigations in gluten toxicity screening, like K562(S)-cell agglutination, A1 and G12 monoclonal antibodies and proteomics. In this paper we want to present the investigative developments in celiac disease. We also want to find whether a new treatment in celiac disease is necessary.
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The prevalence of gluten-related diseases is increasing in an alarming rate. The studies regardin... more The prevalence of gluten-related diseases is increasing in an alarming rate. The studies regarding wheat allergy in the Southeastern and Central Europe are few. The aim of our study was to discuss the effectiveness of serological tests in detecting the prevalence of specific allergens.
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Immunology Letters, 2017
Pancreatic cancer has attracted a great deal of attention owing to the poor outcome, increasing p... more Pancreatic cancer has attracted a great deal of attention owing to the poor outcome, increasing prevalence in the last years and delay diagnosis. Known as a complex disease, it involves genetic mutations, changes in tumour microenvironment and inflammatory component dominated by interleukin-6 and its activated pathways, like Janus Kinase-Signal Transducer and Activator of Translation3, Mitogen Activated Protein Kinase and Androgen receptor. The pro-inflammatory cytokine, plays a central role in oncogenesis, cancer progression, invasiveness, microenvironment changes, treatment resistance, prognosis and associated co morbidities like cachexia and depression. Fulfilling these roles IL-6 requires special attention to understand its complexity in PC development.
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Pediatric Rheumatology, 2015
Background: Our aim was to investigate the prevalence and clinical relevance of inherited complem... more Background: Our aim was to investigate the prevalence and clinical relevance of inherited complement and antibody deficiency states in a large series of patients with various autoimmune rheumatologic diseases (ARD) with juvenile onset.
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Background: Bullous pemphigoid is a subepidermal blistering disorder associated with tissue-bound... more Background: Bullous pemphigoid is a subepidermal blistering disorder associated with tissue-bound and circulating autoantibodies directed mainly to the hemidesmosomal component collagen XVII. While recapitulating the main immunopathological features of the human disease, frank skin blistering does not develop in the absence of skin rubbing in experimental pemphigoid models that have been established in neonatal mice. Moreover, due to their experimental design they only allow for short-term disease observation. In the present study we aimed to establish a model that reproduces the frank skin blistering seen in patients and allows for longer observation times.
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Romanian Journal of Internal Medicine, 2017
Introduction. Heart failure (HF) is characterized by neuroendocrine activation. The cardiac natri... more Introduction. Heart failure (HF) is characterized by neuroendocrine activation. The cardiac natriuretic hormones, including atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP), together with their related pro-peptides (proANP and proBNP) represent a group of peptide hormones produced by the heart. A normal NT-proBNP level has a high negative predictive value for heart failure. The use of NT-proBNP testing is helpful in diagnosing acute HF in the emergency care setting, allowing an early and optimal treatment. The purpose of this study is to assess the prognostic value of NT-proBNP in heart failure in children younger than 3 years old and to establish whether it correlates with the NYHA/Ross functional class and left ventricle systolic function. Methods. We enrolled 24 consecutive children with HF due to congenital heart diseases and dilated cardiomyopathy. The serum levels of NT-proBNP were measured, all patients underwent echocardiography and left ventricle ejection fraction was calculated. Results. The highest median value of NT-proBNP was recorded in patients with cyanotic heart diseases (248.0 fmol/mL), p=0.610. NT-proBNP had a negative correlation with the ejection fraction of the left ventricle: Spearman's rank correlation coefficient was -0.165. Conclusions. NT-proBNP levels correlate with the severity of HF in infants and small children younger than 3 years old with heart failure due to congenital heart diseases and dilated cardiomyopathy.
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Laboratory Medicine (Journal of the American Society for Clinical Pathology) , 2014
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Background and Aims. Two-dimensional (2D) and Doppler echocardiography are the main methods for t... more Background and Aims. Two-dimensional (2D) and Doppler echocardiography are the main methods for the non-invasive evaluation of ventricular function in children. Our study monitored the evaluation of systolic and diastolic function in pediatric patients, using classical echocardiographic parameters and pulsed tissue Doppler parameters, as well as the correlation between these. Methods. The study included 18 healthy children and 9 children diagnosed with congestive heart failure secondary to congenital heart malformations. The parameters of systolic and diastolic function were measured by 2D echocardiography, 2D guided M mode, color and pulsed Doppler, as well as by pulsed tissue Doppler at the level of the mitral and tricuspid annulus. Results. A relaxation alteration pattern or a pseudonormal pattern of E diastolic velocity compared to the A wave was found (E = A; E > A) in the group of subjects with heart failure. E wave deceleration time had significantly increased values in the case of patients with CHF, being correlated with diastolic dysfunction. Left ventricular flow propagation velocity Vp was decreased in patients with heart failure, the E/Vp ratio being maintained relatively constant in subjects with congestive heart failure and healthy subjects, most probably on account of the concomitant change in the E wave. Associations between the severity of systolic dysfunction and the diastolic dysfunction were found in pediatric patients diagnosed with congestive heart failure (Student test, p < 0.05). Conclusions. Tissue Doppler measurements proved to be useful for the evaluation of pediatric patients with altered ventricular geometry secondary to congenital heart disease, systolic-diastolic dysfunction and heart failure.
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Congenital lobar emphysema in infants is a disorder that is detected most often in newborns or yo... more Congenital lobar emphysema in infants is a disorder that is detected most often in newborns or young infants. We report here the case of a 4-month-old infant who at two months suddenly presented upper respiratory infections, treated symptomatically, but evolution showed shortness of breath, wheezing, weight deficit. Thoracic ultrasound revealed left upper lobe hyperinflation causing mediastinal displacement to the right, a slightly reduced blood supply at this level, and a lobar emphysema appearance. Bronchoscopy evidenced a thickening in the left bronchial tree, due to left upper lobe emphysema.
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HIV infection in Romania remains an important public health problem. In this paper we aimed to an... more HIV infection in Romania remains an important public health problem. In this paper we aimed to analyze the current situation of HIV infection in Romania in sociopolitical context, in terms of laboratory diagnosis. HIV epidemic in the years 1980-1990 had serious consequences on the Romanian society. After a period of decline of the infection, the development of new HIV cases in 2011 provided a warning to the Romanian society. The problem can be solved through the close cooperation of all social classes, from health professionals to politicians.
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Progress in molecular medicine has led to the appearance of two scientific branches: pharmacogene... more Progress in molecular medicine has led to the appearance of two scientific branches: pharmacogenetics and pharmacogenomics. The use of genomic and molecular data contained in the patients' genotype has allowed for the development of new personalized pharmaceutical products. Although pharmacogenetics and pharmacogenomics contribute to the production of effective and safe drugs, they raise ethical problems with specific nuances and subtleties and high complexity levels. The most important refer to the informed consent for genetic testing, personal autonomy in relation to primary and/or secondary information resulting from DNA testing, confidentiality, the principle of equality and equity. Ethical problems also concern patient stratification depending on their economic status, given that personalized drugs might be too expensive, prohibitive for some patients, and stratification depending on race or nationality, with the danger of creating " orphan populations ". Discriminatory attitudes can be avoided, primarily through the management of the patients'genetic data under conditions of confidentiality.
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ABSTRACT
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Journal of Labelled Compounds and Radiopharmaceuticals, 2008
ABSTRACT
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Journal of Labelled Compounds and Radiopharmaceuticals, 2008
ABSTRACT
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Annals of the Romanian Society for Cell Biology
In order to overcome the crisis of antibiotic resistance, there is an urgent need for alternative... more In order to overcome the crisis of antibiotic resistance, there is an urgent need for alternative antibacterial agents that have novel mechanisms of action. FtsZ is an attractive target for antibacterial drug discovery because of its widespread conservation in the bacterial kingdom, its absence in the mitochondria of higher eukaryotes and its known biochemical activity and molecular structure. FtsZ plays an essential role in prokaryotic cell division machinery in which undergoes GTP-dependent polymerization midcell and assembles into the dynamic Z-ring at the site of division. The Z-ring acts to initiate Z-ring contraction to form bacterial daughter cells. Although FtsZ shares structural and functional similarity with eukaryotic tubulin, most of the tubulin/microtubule targeting agents do not affect the dynamic assembly of FtsZ, indicating tha t FtsZ can be a selective antibacterial target. In this study, we evaluated the immunogenicity in mice of FtsZ protein from Pseudomonas aerug...
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Annals of the Romanian Society for Cell Biology
Biodegradation is a long process and microorganism communities responsible for this process are c... more Biodegradation is a long process and microorganism communities responsible for this process are complex, mostly composed mainly of fungi and bacteria. Some of these communities, depending on the substrate and micro climatic conditions, may be associated with cyanobacteria, algae and Archaea . Determination of the diversity of microorganisms that inhabit different art works is of major importance for restoration and conservation. The methods used in studying these communities in recent decades can be summarized to a few techniques (DGGE, cultured and uncultured methods, 16S rDNA clone library analysis etc), but these may underestimate the diversity of communities compared to new techniques available (next generation sequencings methods) that provide more close to reality results. In this paper we tried to show the research findings in last few decades that point microorganism communities that populate the world heritage objects and we support the need to investigate these communities...
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Romanian Biotechnological Letters
Bacteria identification through PCR with 16S rDNA gene universal primers was widely used in clini... more Bacteria identification through PCR with 16S rDNA gene universal primers was widely used in clinical, environmental and applied microbiology. In practice, it is a considerable challenge to achieve high sensitivity and a clear negative control at the same time, due to contamination of commercial Taq polymerases with trace of bacterial DNA. In this paper, besides the methods proposed so far to avoid amplification in negative controls, we propose using of 16S rDNA gene universal primer pair (27F and 1407R), which unlike other primer pairs result with a clean control. Additionally, even if the negative control checked by agarose gel is clean, it is still possible presence of detectable PCR product, and we have identified the source of DNA contamination in six commercial Taq polymerases. We tested seven commercial Taq polymerases for presence of trace contaminating DNA and only one Taq polymerase was bacterial DNA free. These data can be useful to eliminate the false positive products wh...
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