Ji Eun Kim | University of British Columbia (original) (raw)

Papers by Ji Eun Kim

Research paper thumbnail of Aflatoxin B1 in poultry: Toxicology, metabolism and prevention

Research in Veterinary Science, 2010

Research paper thumbnail of Chromoplasts ultrastructure and estimated carotene content in root secondary phloem of different carrot varieties

Planta, 2010

There have been few studies on quantifying carotenoid accumulation in carrots, and none have take... more There have been few studies on quantifying carotenoid accumulation in carrots, and none have taken the comparative approach. The abundance and distribution of carotenes in carrot roots of three varieties, white, orange, and high carotene mass (HCM) were compared using light and transmission electron microscopy (TEM). Light microscopy has indicated that, in all three varieties, carotenes were most abundant in the secondary phloem and this area was selected for further TEM analysis. While carotenes were extracted during the fixation process for TEM, the high-pressure freezing technique we employed preserved the spaces (CS) left behind by the extracted carotene crystals. Chromoplasts from the HCM variety contained significantly (P \ 0.05) more CS than chromoplasts from the orange variety. Chromoplasts from the white variety had few or no CS. There was no significant difference between the HCM and orange varieties in the number of chromoplasts per unit area, but the white variety had significantly (P \ 0.05) fewer chromoplasts than the other two varieties. A large number of starch-filled amyloplasts was observed in secondary phloem of the white variety but these were not found in the other two varieties. The results from this comparative approach clearly define the subcellular localization of carotenoids in carrot roots and suggest that while the HCM genotype was selectively bred for increased carotene content, this selection did not lead to increased numbers of carotene-containing chromoplasts but rather greater accumulation of carotene per chromoplast. Furthermore, the results confirm that roots of the white carrot variety retain residual amounts of carotene.

Research paper thumbnail of Characterization of cecal microbiota of the emu (Dromaius novaehollandiae)

Veterinary Microbiology, 2013

Emus (Dromaius novaehollandiae), large flightless ratites native to Australia, are farmed for the... more Emus (Dromaius novaehollandiae), large flightless ratites native to Australia, are farmed for their fat and meat. They are omnivorous and feed on a wide variety of plants and insects. Despite having a relatively simple gastrointestinal tract and a short digesta retention time, emus are able to digest a significant portion of the ingested dietary neutral detergent fibre. However, nothing is known about the microbial diversity in their gastrointestinal tract. In this study, we evaluated the phylogenetic diversity of the cecal microbiota of four emus (2 males, 2 females) that were fed a barley-alfalfa-canola based diet, using 454 pyrosequencing after amplification for V3-V5 region of bacterial 16S rRNA gene. Emus were slaughtered in early November, just prior to the onset of their breeding season, but after the seasonal decline in their feed intake had begun. A total of 822 operational taxonomic units (OTUs) (335.3 AE 70.5 OTUs/sample) belonging to 9 bacterial phyla were identified. The most predominant bacterial phyla were Bacteroidetes ($57% of total classified diversity), Proteobacteria ($24%), Fusobacteria ($11.3%), and Firmicutes ($7%). Our results indicate that the emus' ceca may have a higher microbial richness (Chao1: 624 AE 170 OTUs, and ACE: 586 AE 161 OTUs) than other species of birds, but they have a lower microbial diversity (Shannon diversity index: 3.4 AE 0.2, Simpson index: 0.79 AE 0.02), possibly reflecting their decrease feed intake. This is the first study to characterize the microbial community of the gastrointestinal tract of a ratite using pyrosequencing, providing a baseline for further study.

Research paper thumbnail of Genetic Variation within and Between Domesticated Chinook Salmon, Oncorhynchus tshawytscha, Strains and their Progenitor Populations

Environmental Biology of Fishes, 2000

Domesticated chinook salmon strains in British Columbia (BC), Canada are believed to have origina... more Domesticated chinook salmon strains in British Columbia (BC), Canada are believed to have originated primarily from populations of the Big Qualicum (BQ) River and Robertson Creek (RC) on Vancouver Island in the early 1980s. The number of parental fish that gave rise to the domesticated strains and their subsequent breeding history during approximately five ensuing generations of domestication were not documented. Genetic variation at 13 microsatellite loci was examined in samples from two domesticated strains and the two progenitor populations to determine the genetic relationships among them. The domesticated strains had lower allelic diversity and tended to have lower levels of expected heterozygosity than did the BQ and RC progenitor populations. Only three alleles over all 13 loci were detected in the domesticated strains that were not present in the BQ and RC samples, whereas the progenitor strains possessed over 25 (BQ) and 43 (RC) private alleles. Genetic distance and F ST values also indicated a closer relationship of the domesticated strains with the BQ than the RC population. One domesticated strain had a significant excess of heterozygosity compared with that expected under conditions of mutation-drift equilibrium, indicative of a recent genetic bottleneck. Genetic differentiation between the domesticated strains was as great as that distinguishing them from the progenitor populations, indicating that the genetic base of domesticated chinook salmon could be increased by hybridization. The existence of genetically distinct domesticated strains of chinook salmon in coastal BC generates the need for an evaluation of potential genetic interactions between domesticated escapees and natural spawning populations.

Research paper thumbnail of Aflatoxin B1 in poultry: Toxicology, metabolism and prevention

Research in Veterinary Science, 2010

Research paper thumbnail of Chromoplasts ultrastructure and estimated carotene content in root secondary phloem of different carrot varieties

Planta, 2010

There have been few studies on quantifying carotenoid accumulation in carrots, and none have take... more There have been few studies on quantifying carotenoid accumulation in carrots, and none have taken the comparative approach. The abundance and distribution of carotenes in carrot roots of three varieties, white, orange, and high carotene mass (HCM) were compared using light and transmission electron microscopy (TEM). Light microscopy has indicated that, in all three varieties, carotenes were most abundant in the secondary phloem and this area was selected for further TEM analysis. While carotenes were extracted during the fixation process for TEM, the high-pressure freezing technique we employed preserved the spaces (CS) left behind by the extracted carotene crystals. Chromoplasts from the HCM variety contained significantly (P \ 0.05) more CS than chromoplasts from the orange variety. Chromoplasts from the white variety had few or no CS. There was no significant difference between the HCM and orange varieties in the number of chromoplasts per unit area, but the white variety had significantly (P \ 0.05) fewer chromoplasts than the other two varieties. A large number of starch-filled amyloplasts was observed in secondary phloem of the white variety but these were not found in the other two varieties. The results from this comparative approach clearly define the subcellular localization of carotenoids in carrot roots and suggest that while the HCM genotype was selectively bred for increased carotene content, this selection did not lead to increased numbers of carotene-containing chromoplasts but rather greater accumulation of carotene per chromoplast. Furthermore, the results confirm that roots of the white carrot variety retain residual amounts of carotene.

Research paper thumbnail of Characterization of cecal microbiota of the emu (Dromaius novaehollandiae)

Veterinary Microbiology, 2013

Emus (Dromaius novaehollandiae), large flightless ratites native to Australia, are farmed for the... more Emus (Dromaius novaehollandiae), large flightless ratites native to Australia, are farmed for their fat and meat. They are omnivorous and feed on a wide variety of plants and insects. Despite having a relatively simple gastrointestinal tract and a short digesta retention time, emus are able to digest a significant portion of the ingested dietary neutral detergent fibre. However, nothing is known about the microbial diversity in their gastrointestinal tract. In this study, we evaluated the phylogenetic diversity of the cecal microbiota of four emus (2 males, 2 females) that were fed a barley-alfalfa-canola based diet, using 454 pyrosequencing after amplification for V3-V5 region of bacterial 16S rRNA gene. Emus were slaughtered in early November, just prior to the onset of their breeding season, but after the seasonal decline in their feed intake had begun. A total of 822 operational taxonomic units (OTUs) (335.3 AE 70.5 OTUs/sample) belonging to 9 bacterial phyla were identified. The most predominant bacterial phyla were Bacteroidetes ($57% of total classified diversity), Proteobacteria ($24%), Fusobacteria ($11.3%), and Firmicutes ($7%). Our results indicate that the emus' ceca may have a higher microbial richness (Chao1: 624 AE 170 OTUs, and ACE: 586 AE 161 OTUs) than other species of birds, but they have a lower microbial diversity (Shannon diversity index: 3.4 AE 0.2, Simpson index: 0.79 AE 0.02), possibly reflecting their decrease feed intake. This is the first study to characterize the microbial community of the gastrointestinal tract of a ratite using pyrosequencing, providing a baseline for further study.

Research paper thumbnail of Genetic Variation within and Between Domesticated Chinook Salmon, Oncorhynchus tshawytscha, Strains and their Progenitor Populations

Environmental Biology of Fishes, 2000

Domesticated chinook salmon strains in British Columbia (BC), Canada are believed to have origina... more Domesticated chinook salmon strains in British Columbia (BC), Canada are believed to have originated primarily from populations of the Big Qualicum (BQ) River and Robertson Creek (RC) on Vancouver Island in the early 1980s. The number of parental fish that gave rise to the domesticated strains and their subsequent breeding history during approximately five ensuing generations of domestication were not documented. Genetic variation at 13 microsatellite loci was examined in samples from two domesticated strains and the two progenitor populations to determine the genetic relationships among them. The domesticated strains had lower allelic diversity and tended to have lower levels of expected heterozygosity than did the BQ and RC progenitor populations. Only three alleles over all 13 loci were detected in the domesticated strains that were not present in the BQ and RC samples, whereas the progenitor strains possessed over 25 (BQ) and 43 (RC) private alleles. Genetic distance and F ST values also indicated a closer relationship of the domesticated strains with the BQ than the RC population. One domesticated strain had a significant excess of heterozygosity compared with that expected under conditions of mutation-drift equilibrium, indicative of a recent genetic bottleneck. Genetic differentiation between the domesticated strains was as great as that distinguishing them from the progenitor populations, indicating that the genetic base of domesticated chinook salmon could be increased by hybridization. The existence of genetically distinct domesticated strains of chinook salmon in coastal BC generates the need for an evaluation of potential genetic interactions between domesticated escapees and natural spawning populations.