Jorge Huete | Universidad Centroamericana (UCA) Nicaragua (original) (raw)

Jorge Huete

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Papers by Jorge Huete

Research paper thumbnail of Will Nicaragua’s Interoceanic Canal Result in an Environmental Catastrophe for Central America? Jorge A. Huete-Perez †*, Jose G. Tundisi ‡, and Pedro J. J. Alvarez †§

Research paper thumbnail of Will Nicaragua’s Interoceanic Canal Result in an Environmental Catastrophe for Central America? Jorge A. Huete-Perez †*, Jose G. Tundisi ‡, and Pedro J. J. Alvarez †§

Research paper thumbnail of Conservation: Nicaragua Canal could wreak environmental ruin Axel Meyer & Jorge A. Huete-Pérez

Research paper thumbnail of Latin American Science: Much Work Remains

Research paper thumbnail of Marine genome resource sustainability in Central America

Research paper thumbnail of Biotech Gap Between North and South

Research paper thumbnail of New Scientific Society in Nicaragua

Research paper thumbnail of Nicaragua's Research Agenda

Research paper thumbnail of A genetic study of the Identifiler™ System 15 STR loci in the general population of Nicaragua, Central America

Legal Medicine, 2011

Allele frequencies for the 15 short tandem repeats loci D8S1179, D21S11, D7S820, CSF1PO, D3S1358,... more Allele frequencies for the 15 short tandem repeats loci D8S1179, D21S11, D7S820, CSF1PO, D3S1358, TH01, D13S317, D16S539, D2S1338, D19S433, vWA, TPOX, D18S51, D5S818 and FGA (AmpFℓSTR® Identifiler™ PCR Amplification Kit, Applied Biosystems) were determined in a sample of 322 unrelated individuals from Nicaragua. Statistical analyses were performed using PowerStats v12 and Genepop v4.0. All loci are in Hardy–Weinberg equilibrium and show high discrimination for paternity analysis and forensic genetic applications.

Research paper thumbnail of Percepciones sobre biotecnología en Nicaragua

Research paper thumbnail of DNA Barcode, una alternativa para identificar especies del Complejo Midas Cichlidae en Nicaragua

Research paper thumbnail of Tendencias actuales de la biotecnología en Nicaragua

Encuentro, 2006

Nicaragua. Se destaca el reciente auge de esta área del conocimiento basándose en información obt... more Nicaragua. Se destaca el reciente auge de esta área del conocimiento basándose en información obtenida de los tres Congresos Nicaragüenses de Biotecnología (2002, 2004 y 2006). Se describen, en particular, las actividades y temáticas abordadas en el tercer Congreso, resaltando las ponencias más sobresalientes. Al mismo tiempo, se discute la potencialidad de este sector para la economía nacional. Esta revisión del tema, a manera de diagnóstico de la situación actual, podría contribuir a la toma de decisiones sobre el desarrollo de políticas de promoción de la investigación científica.

Research paper thumbnail of P. putida formaldehyde dehydrogenase. An alcohol dehydrogenase masquerading as an aldehyde dehydrogenase

… medicine and biology, 1997

Research paper thumbnail of Determinación de daño genético en comerciantes de plaguicidas en el departamento de Matagalpa

Research paper thumbnail of Monitoreo de arroz, soya y maíz comercializados en Nicaragua para detección de material transgénico

Migración y trata de …, 2007

Research paper thumbnail of Agricultural IP and the public sector

Research paper thumbnail of Allele frequencies for markers CSF1PO, TPOX, TH01, F13A01, FESFPS, vWA, D16S539, D7S820, D13S317 in the general population of Nicaragua

Journal of Forensic Sciences, 2004

Research paper thumbnail of Genomic and proteomic approaches for Chagas' disease: critical analysis of diagnostic methods

Expert Review of Molecular Diagnostics, 2005

Trypanosoma cruzi is the etiologic agent of Chagas&am... more Trypanosoma cruzi is the etiologic agent of Chagas' disease, a chronic inflammatory condition that results in heart and digestive complications. The first draft of the parasite genome is now complete and it is expected that, along with the published genomic and proteomic analyses discussed herein, it will lead to the identification of crucial genes and proteins directly associated with disease. This article reviews the current research trends addressing host-parasite interaction, parasite genetic variability and diagnosis. These advances will certainly bring about major developments not only in our understanding of Trypanosoma cruzi biology, but also in the application of new technologies to disease prevention and control.

Research paper thumbnail of Bioprospección de enzimas de restricción en bacterias de suelos y ambientes volcánicos de Nicaragua

Research paper thumbnail of Preliminary X‐ray crystallographic analysis of Tritrichomonas foetus inosine‐5′‐monophosphate dehydrogenase

Proteins-structure Function and Bioinformatics, 1995

Analysis of the genomic sequences of Escherichia coli and Salmonella typhimurium has revealed the... more Analysis of the genomic sequences of Escherichia coli and Salmonella typhimurium has revealed the presence of several homologues of the well studied citrate synthase (CS). One of these homologues has been shown to code for 2-methylcitrate synthase (2-MCS) activity. 2-MCS catalyzes one of the steps in the 2-methylcitric acid cycle found in these organisms for the degradation of propionate to pyruvate and succinate. In the present work, the gene coding for 2-MCS from S. typhimurium (StPrpC) was cloned in pRSET-C vector and overexpressed in E. coli. The protein was purified to homogeneity using Ni-NTA affinity chromatography. The purified protein was crystallized using the microbatch-under-oil method. The StPrpC crystals diffracted X-rays to 2.4 Å resolution and belonged to the triclinic space group P1, with unit-cell parameters a = 92. 068, b = 118.159, c = 120.659 Å , = 60.84, = 67.77, = 81.92 . Computation of rotation functions using the X-ray diffraction data shows that the protein is likely to be a decamer of identical subunits, unlike CSs, which are dimers or hexamers.

Research paper thumbnail of Will Nicaragua’s Interoceanic Canal Result in an Environmental Catastrophe for Central America? Jorge A. Huete-Perez †*, Jose G. Tundisi ‡, and Pedro J. J. Alvarez †§

Research paper thumbnail of Will Nicaragua’s Interoceanic Canal Result in an Environmental Catastrophe for Central America? Jorge A. Huete-Perez †*, Jose G. Tundisi ‡, and Pedro J. J. Alvarez †§

Research paper thumbnail of Conservation: Nicaragua Canal could wreak environmental ruin Axel Meyer & Jorge A. Huete-Pérez

Research paper thumbnail of Latin American Science: Much Work Remains

Research paper thumbnail of Marine genome resource sustainability in Central America

Research paper thumbnail of Biotech Gap Between North and South

Research paper thumbnail of New Scientific Society in Nicaragua

Research paper thumbnail of Nicaragua's Research Agenda

Research paper thumbnail of A genetic study of the Identifiler™ System 15 STR loci in the general population of Nicaragua, Central America

Legal Medicine, 2011

Allele frequencies for the 15 short tandem repeats loci D8S1179, D21S11, D7S820, CSF1PO, D3S1358,... more Allele frequencies for the 15 short tandem repeats loci D8S1179, D21S11, D7S820, CSF1PO, D3S1358, TH01, D13S317, D16S539, D2S1338, D19S433, vWA, TPOX, D18S51, D5S818 and FGA (AmpFℓSTR® Identifiler™ PCR Amplification Kit, Applied Biosystems) were determined in a sample of 322 unrelated individuals from Nicaragua. Statistical analyses were performed using PowerStats v12 and Genepop v4.0. All loci are in Hardy–Weinberg equilibrium and show high discrimination for paternity analysis and forensic genetic applications.

Research paper thumbnail of Percepciones sobre biotecnología en Nicaragua

Research paper thumbnail of DNA Barcode, una alternativa para identificar especies del Complejo Midas Cichlidae en Nicaragua

Research paper thumbnail of Tendencias actuales de la biotecnología en Nicaragua

Encuentro, 2006

Nicaragua. Se destaca el reciente auge de esta área del conocimiento basándose en información obt... more Nicaragua. Se destaca el reciente auge de esta área del conocimiento basándose en información obtenida de los tres Congresos Nicaragüenses de Biotecnología (2002, 2004 y 2006). Se describen, en particular, las actividades y temáticas abordadas en el tercer Congreso, resaltando las ponencias más sobresalientes. Al mismo tiempo, se discute la potencialidad de este sector para la economía nacional. Esta revisión del tema, a manera de diagnóstico de la situación actual, podría contribuir a la toma de decisiones sobre el desarrollo de políticas de promoción de la investigación científica.

Research paper thumbnail of P. putida formaldehyde dehydrogenase. An alcohol dehydrogenase masquerading as an aldehyde dehydrogenase

… medicine and biology, 1997

Research paper thumbnail of Determinación de daño genético en comerciantes de plaguicidas en el departamento de Matagalpa

Research paper thumbnail of Monitoreo de arroz, soya y maíz comercializados en Nicaragua para detección de material transgénico

Migración y trata de …, 2007

Research paper thumbnail of Agricultural IP and the public sector

Research paper thumbnail of Allele frequencies for markers CSF1PO, TPOX, TH01, F13A01, FESFPS, vWA, D16S539, D7S820, D13S317 in the general population of Nicaragua

Journal of Forensic Sciences, 2004

Research paper thumbnail of Genomic and proteomic approaches for Chagas' disease: critical analysis of diagnostic methods

Expert Review of Molecular Diagnostics, 2005

Trypanosoma cruzi is the etiologic agent of Chagas&am... more Trypanosoma cruzi is the etiologic agent of Chagas' disease, a chronic inflammatory condition that results in heart and digestive complications. The first draft of the parasite genome is now complete and it is expected that, along with the published genomic and proteomic analyses discussed herein, it will lead to the identification of crucial genes and proteins directly associated with disease. This article reviews the current research trends addressing host-parasite interaction, parasite genetic variability and diagnosis. These advances will certainly bring about major developments not only in our understanding of Trypanosoma cruzi biology, but also in the application of new technologies to disease prevention and control.

Research paper thumbnail of Bioprospección de enzimas de restricción en bacterias de suelos y ambientes volcánicos de Nicaragua

Research paper thumbnail of Preliminary X‐ray crystallographic analysis of Tritrichomonas foetus inosine‐5′‐monophosphate dehydrogenase

Proteins-structure Function and Bioinformatics, 1995

Analysis of the genomic sequences of Escherichia coli and Salmonella typhimurium has revealed the... more Analysis of the genomic sequences of Escherichia coli and Salmonella typhimurium has revealed the presence of several homologues of the well studied citrate synthase (CS). One of these homologues has been shown to code for 2-methylcitrate synthase (2-MCS) activity. 2-MCS catalyzes one of the steps in the 2-methylcitric acid cycle found in these organisms for the degradation of propionate to pyruvate and succinate. In the present work, the gene coding for 2-MCS from S. typhimurium (StPrpC) was cloned in pRSET-C vector and overexpressed in E. coli. The protein was purified to homogeneity using Ni-NTA affinity chromatography. The purified protein was crystallized using the microbatch-under-oil method. The StPrpC crystals diffracted X-rays to 2.4 Å resolution and belonged to the triclinic space group P1, with unit-cell parameters a = 92. 068, b = 118.159, c = 120.659 Å , = 60.84, = 67.77, = 81.92 . Computation of rotation functions using the X-ray diffraction data shows that the protein is likely to be a decamer of identical subunits, unlike CSs, which are dimers or hexamers.

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