Zhe Yan | University of Colorado Denver (original) (raw)
Address: Sacramento, California, United States
less
Uploads
Papers by Zhe Yan
Peptides Across the Pacific: The Proceedings of the Twenty-Third American and the Sixth International Peptide Symposium, 2013
We extend to you a warm and sunny Aloha in celebration of the 23 rd American Peptide Symposium an... more We extend to you a warm and sunny Aloha in celebration of the 23 rd American Peptide Symposium and the 6 th International Symposium. The meeting theme, Peptides Across the Pacific, embraced the spirit of the scientific and social program. Peptides Across the Pacific encompassed the important role that peptide science currently plays in so many disciplines and explored the potential impact peptides can make in scientific fields that have yet to realize the utility of these wonderful molecules. The scientific program for 2013 was framed by distinguished lectures delivered by two renowned peptide chemists.
Understanding Biology Using Peptides
Biophysical Journal, 2009
Journal of Structural Biology, 2006
The Spike (S) protein of SARS-coronavirus (SARS-CoV) mediates viral entry into host cells. It con... more The Spike (S) protein of SARS-coronavirus (SARS-CoV) mediates viral entry into host cells. It contains two heptad repeat regions, denoted HRN and HRC. We have identiWed the location of the two interacting HR regions that form the six-helix bundle (B. Tripet, et al,
Chemical Biology & Drug Design, 2009
Severe acute respiratory syndrome (SARS) is an infectious disease caused by a novel coronavirus t... more Severe acute respiratory syndrome (SARS) is an infectious disease caused by a novel coronavirus that cost nearly 800 lives. While there have been no recent outbreaks of the disease, the threat remains as SARS coronavirus (SARS-CoV) like strains still exist in animal reservoirs. Therefore, the development of a vaccine against SARS is in grave need. Here, we have designed and produced a prototypic SARS vaccine: a self-assembling polypeptide nanoparticle that repetitively displays a SARS B-cell epitope from the C-terminal heptad repeat of the virus’ spike protein. Biophysical analyses with circular dichroism, transmission electron microscopy and dynamic light scattering confirmed the computational design showing α-helcial nanoparticles with sizes of about 25 nm. Immunization experiments with no adjuvants were performed with BALB/c mice. An investigation of the binding properties of the elicited antibodies showed that they were highly conformation specific for the coiled-coil epitope because they specifically recognized the native trimeric conformation of C-terminal heptad repeat region. Consequently, the antisera exhibited neutralization activity in an in vitro infection inhibition assay. We conclude that these peptide nanoparticles represent a promising platform for vaccine design, in particular for diseases that are characterized by neutralizing epitopes with coiled-coil conformation such as SARS-CoV or other enveloped viruses.
Peptides Across the Pacific: The Proceedings of the Twenty-Third American and the Sixth International Peptide Symposium, 2013
We extend to you a warm and sunny Aloha in celebration of the 23 rd American Peptide Symposium an... more We extend to you a warm and sunny Aloha in celebration of the 23 rd American Peptide Symposium and the 6 th International Symposium. The meeting theme, Peptides Across the Pacific, embraced the spirit of the scientific and social program. Peptides Across the Pacific encompassed the important role that peptide science currently plays in so many disciplines and explored the potential impact peptides can make in scientific fields that have yet to realize the utility of these wonderful molecules. The scientific program for 2013 was framed by distinguished lectures delivered by two renowned peptide chemists.
Understanding Biology Using Peptides
Biophysical Journal, 2009
Journal of Structural Biology, 2006
The Spike (S) protein of SARS-coronavirus (SARS-CoV) mediates viral entry into host cells. It con... more The Spike (S) protein of SARS-coronavirus (SARS-CoV) mediates viral entry into host cells. It contains two heptad repeat regions, denoted HRN and HRC. We have identiWed the location of the two interacting HR regions that form the six-helix bundle (B. Tripet, et al,
Chemical Biology & Drug Design, 2009
Severe acute respiratory syndrome (SARS) is an infectious disease caused by a novel coronavirus t... more Severe acute respiratory syndrome (SARS) is an infectious disease caused by a novel coronavirus that cost nearly 800 lives. While there have been no recent outbreaks of the disease, the threat remains as SARS coronavirus (SARS-CoV) like strains still exist in animal reservoirs. Therefore, the development of a vaccine against SARS is in grave need. Here, we have designed and produced a prototypic SARS vaccine: a self-assembling polypeptide nanoparticle that repetitively displays a SARS B-cell epitope from the C-terminal heptad repeat of the virus’ spike protein. Biophysical analyses with circular dichroism, transmission electron microscopy and dynamic light scattering confirmed the computational design showing α-helcial nanoparticles with sizes of about 25 nm. Immunization experiments with no adjuvants were performed with BALB/c mice. An investigation of the binding properties of the elicited antibodies showed that they were highly conformation specific for the coiled-coil epitope because they specifically recognized the native trimeric conformation of C-terminal heptad repeat region. Consequently, the antisera exhibited neutralization activity in an in vitro infection inhibition assay. We conclude that these peptide nanoparticles represent a promising platform for vaccine design, in particular for diseases that are characterized by neutralizing epitopes with coiled-coil conformation such as SARS-CoV or other enveloped viruses.