Hervé Rogez - Profile on Academia.edu (original) (raw)

Papers by Hervé Rogez

Spontaneous postharvest fermentation of açai (Euterpe oleracea) fruit

Postharvest Biology and Technology, 2013

Journal of the Science of Food and Agriculture, 2007

The total phenolics, anthocyanins, flavan 3-ols, carotenoids and antioxidant capacity of mashua (... more The total phenolics, anthocyanins, flavan 3-ols, carotenoids and antioxidant capacity of mashua (Tropaeolum tuberosum Ruiz & Pavón) tubers from 10 yellow and purple cultivars were determined at different maturity stages (5-7.5 months after planting) and sunning post-harvest storage periods (7-35 days). Both the antioxidant capacity (ORAC and ABTS assays) and the content of the bioactive compounds tested varied markedly between cultivars. Purple varieties reached the highest antioxidant capacity during tuber development (271-446 µmol Trolox equiv g −1 DM, ORAC assay). The kinetics of accumulation or disappearance of the bioactive compounds tested during maturation was dependent both on the cultivar and on the compound considered. For anthocyanins, there was a marked increase during maturation in all the purple cultivars. During the sunning postharvest storage, the changes in antioxidant capacity (ABTS assay) and content of the bioactive compounds tested also varied between cultivars. A marked decrease in anthocyanins was observed for the anthocyanin-containing cultivars. In general, the correlation between antioxidant capacity and the content of bioactive compounds varied markedly between cultivars. Antioxidant capacity in purple varieties correlated with total phenolics or flavan 3-ols while only in some yellow varieties antioxidant capacity correlated with total phenolics.

Tecnologias para Inovação nas cadeias Euterpe - Chapter 9

DRYING OF MURUCIZEIRO LEAVES (Byrsonima crassifolia) IN THIN LAYER AT DIFFERENT CONDITIONS

Byrsonima crassifolia, known in Brazil as murucizeiro, is a productive tree, present in all the A... more Byrsonima crassifolia, known in Brazil as murucizeiro, is a productive tree, present in all the Amazonian Region and in Central America. Its two main products, simultaneously and rationally exploitable are the fruits and the leaves, the last ones having a high antioxidant capacity. Due to the interest for their antioxidant capacity, the drying process of the murucizeiro leaves has been

Anticonvulsant properties of Euterpe oleracea in mice

Neurochemistry international, 2015

Açai (Euterpe oleracea Mart.), a highly consumed fruit in Amazon,is from a common palm with remar... more Açai (Euterpe oleracea Mart.), a highly consumed fruit in Amazon,is from a common palm with remarkable antioxidant properties. Because oxidative stress and seizures are intimately linked, this study investigated the potential neuroprotective and anticonvulsant effects of commercial clarified açai juice (EO). EO did not alter spontaneous locomotor activity. Four doses of EO were sufficient to increase latencies to both first myoclonic jerk and first generalized tonic-clonic seizure and significantly decrease the total duration of tonic-clonic seizures caused by pentylenetetrazol administration. Also, electrocortical alterations provoked by pentylenetetrazol were prevented, significantly decreasing amplitude of discharges and frequencies above 50 Hz. EO was also able to completely prevent lipid peroxidation in the cerebral cortex, showing a potent direct scavenging property. These results demonstrate for the first time that E. oleracea significantly protects against seizures and seizu...

Food Chemistry, 2015

The present study investigated the phenolic profiles of the pulp and peel of nine plantain cultiv... more The present study investigated the phenolic profiles of the pulp and peel of nine plantain cultivars and compared them to those of two dessert bananas of commercial interest (Grand Nain and Gros Michel), alongside a newly created hybrid, resistant to black sigatoka disease (F568). Identification and quantification of phenolic compounds were performed by means of HPLC-ESI-HR-MS and HPLC-DAD. Hydroxycinnamic acids, particularly ferulic acid-hexoside with 4.4-85.1 lg/g of dry weight, dominated in the plantain pulp and showed a large diversity among cultivars. Flavonol glycosides were predominant in plantain peels, rutin (242.2-618.7 lg/g of dry weight) being the most abundant. A principal component analysis on the whole data revealed that the phenolic profiles of the hybrid, the dessert bananas and the pure plantains differed from each other. Plantain pulps and peels appeared as good sources of phenolics, which could be involved in the health benefits associated with their current applications.

Separation and Purification Technology, 2007

Adsorption on macroporous resins was tested as a means of purifying the phenolic compounds from c... more Adsorption on macroporous resins was tested as a means of purifying the phenolic compounds from crude extracts of Inga edulis leaves. The experimental design methodology was used to optimise this process. The variables tested were the water proportion (50, 70 and 90%; v/v) in the hydroalcoholic extract solution, its pH value (2.0, 3.5 and 5.0) and the type of adsorbent used (XAD-7, XAD-16, EXA-90 and EXA-118). The time of contact was fixed at 2 h on the basis of kinetic studies. Statistically significant differences (p < 0.05) were obtained for the water proportion and the type of adsorbent, whereas the pH of the extract had no influence on the efficiency of adsorption. In all conditions, the XAD-7 resin gave the best results, reaching 239 mg equivalent phenolics per g resin , and XAD-16 the worst ones. Sorption isotherms were constructed for these two resins under optimal ethanol conditions and fitted well to the Freundlich and Langmuir models (R 2 > 0.95). The optimized results will be used in the design of fixed-bed adsorption systems for the purification and concentration of phenolic compounds from I. edulis leaves.

Separation and Purification Technology, 2007

The extraction of phenolic compounds from Inga edulis leaves was optimized by the simultaneous ma... more The extraction of phenolic compounds from Inga edulis leaves was optimized by the simultaneous maximization of the yield in total phenolics (TP), total flavanoids (TFA), and total flavonols (TFO), using the response surface methodology (RSM). A first set of experiments allowed identifying the temperature, the time of contact and the ethanol proportion in the extraction solution, as the main variables affecting the extraction efficiency. A rotatable central composite design consisting of 18 experimental runs with three replicates at the centre point was then applied and a second-order polynomial model was used to describe the experimental data regarding TP, TFA and TFO. The experimental results fitted well to the model and more than 85% of the variability was explained. TP, TFA and TFO showed different patterns of extractability, with significant variation in the linear, quadratic, and interaction effects of the independent variables. The optimized conditions were 86.8% ethanol, 58.2 • C and a time of contact of 46.8 min. The corresponding predicted values were 134.6 mg gallic acid equiv./g dry matter (DM), 26.0 mg catechin equiv./g DM and 13.8 mg rutin equiv./g DM, for TP, TFA and TFO, respectively. The experimental values agreed with those predicted within a 95% confidence interval, thus indicating the suitability of RSM in optimizing the extraction of phenolics from I. edulis.

Antioxidant properties of açai (Euterpe oleracea) in human plasma

Açai is a very popular polyphenol-rich fruit juice in the Amazonian region. In order to evaluate ... more Açai is a very popular polyphenol-rich fruit juice in the Amazonian region. In order to evaluate its effects on blood oxidative level, 350 ml açai juice were added to the daily diet of 30 male volunteers (aged 41±8.9 years) during 28 days. Fasting blood samples were obtained by vein puncture on day 0, 10, 20 and 28 and LDLs were

Immunomodulatory action of Copaifera spp oleoresins on cytokine production by human monocytes

Biomedicine & Pharmacotherapy, 2015

Copaifera spp oleoresins have been used in folk medicine for centuries; nevertheless, its immunom... more Copaifera spp oleoresins have been used in folk medicine for centuries; nevertheless, its immunomodulatory action has not been investigated. Thus, the goal of this study was to characterize different oleoresins and to verify their action on human monocytes regarding pro- and anti-inflammatory cytokine production (TNF-α and IL-10, respectively). The chemical composition of Brazilian Copaifera reticulata, Copaifera duckey and Copaifera multijuga oleoresins was analyzed by HPLC-MS. Cell viability was assessed by MTT method after incubation of cells with Copaifera spp. Noncytotoxic concentrations of oleoresins were incubated with human monocytes from healthy donors, and cytokine production was determined by ELISA. HPLC-MS analysis for terpenes allowed the identification of six diterpene acids and one sesquiterpene acid. Oleoresins exerted no cytotoxic effects on human monocytes. All oleoresins had a similar profile: LPS-induced TNF-α production was maintained by oleoresins, while a significant inhibitory action on IL-10 production was seen. Copaifera oleoresins seemed to exert an activator profile on human monocytes without affecting cell viability. Such effect may be due to the presence of either diterpene or sesquiterpene acids; however, further studies are necessary to determine the involvement of such compounds in Copaifera immunomodulatory effects.

Journal of The Brazilian Chemical Society, 2007

Um extrato metanol-água das folhas de Inga edulis foi fracionado para identificar os compostos po... more Um extrato metanol-água das folhas de Inga edulis foi fracionado para identificar os compostos polifenólicos. Os compostos identificados foram o acido gálico, a catequina, a epicatequina, a miricetina-3-ramnopiranosídeo, a quercetina-3-glucopiranosídeo e a quercetina-3-ramnopiranosídeo. A capacidade antioxidante do extrato e dos polifenóis puros foi medida pelo teste ORAC e comparada com o teor em fenólicos totais (TP). O extrato bruto seco apresentou valores de ORAC (11.16 mmol TE per g) e TP (496.5 mg GAE per g) muito altos. Os compostos identificados foram responsáveis, respectivamente, por 9.53 % e 12.10 % dos valores ORAC e de TP do extrato de folhas de Inga edulis.

Food Chemistry, 2008

Many plants used in Amazonian folk medicine present a high antioxidant activity. In this study, t... more Many plants used in Amazonian folk medicine present a high antioxidant activity. In this study, the antioxidant activities of four largely used plants, namely Byrsonima crassifolia, Davilla kunthii, Davilla rugosa and Inga edulis, were evaluated, using methanolic extracts of their leaves, fruits and bark and several different in vitro tests, based either on the capacity to scavenge free radicals (ORAC,

Nutritional composition and antioxidant properties of the sim fruit (Rhodomyrtus tomentosa)

Food Chemistry, 2015

In this study, detailed chemical properties of sim (Rhodomyrtus tomentosa (Ait.) Hassk.) fruit in... more In this study, detailed chemical properties of sim (Rhodomyrtus tomentosa (Ait.) Hassk.) fruit including nutritional composition, phenolic content and antioxidant capacity were determined for the first time. A 150g serving of sim fruit contained high levels of dietary fibre (69.94-87.43% of Recommended Daily Intake (RDI)), α-tocopherol (38.90-51.87% RDI), manganese (&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;gt;100% RDI), and copper (44.44% RDI) but low levels of protein (2.63% RDI), lipid (1.59-3.5% RDI), and sugars (5.65% RDI). The predominant fatty acid in the sim fruit sample was linoleic acid (75.36% of total fatty acids). Interestingly, total phenolics (49.21±0.35mg gallic acid equivalent (GAE)/g dry weight (DW)) were particularly high and resulted in a high antioxidant capacity (431.17±14.56μmol Trolox equivalent (TE)/g DW). These results, together with our recent discovery of high amount of piceatannol, a stilbene with potent biological activities, highlight the potential of sim, an under-utilised plant species from South-East Asia, as a new source of health-promoting compounds including dietary fibres, essential fatty acids, and phenolic compounds.

Equilibria, Kinetics, and Mechanisms for the Adsorption of Four Classes of Phenolic Compounds onto Synthetic Resins

Separation Science and Technology, 2010

The adsorption of five phenolic compounds of four different classes from aqueous batch solutions ... more The adsorption of five phenolic compounds of four different classes from aqueous batch solutions onto four styrene-divinylbenzene and acrylic resins (EXA 90, EXA 118, XAD 7, and XAD 16) was investigated regarding their equilibria, kinetics and surface-energy heterogeneity, and mechanisms of adsorption. The experimental equilibrium data were very well fitted to Langmuir and Freundlich models (R &amp;amp;amp;gt; 0.900). Three kinetic models

Separation and Purification Technology, 2007

Optimal conditions for the extraction of phenolic compounds, anthocyanins, flavanols and ORAC ant... more Optimal conditions for the extraction of phenolic compounds, anthocyanins, flavanols and ORAC antioxidant activity for two mashua genotypes were determined using the conventional liquid-solid method. Phenolic contents and antioxidant activity were affected by the type of solvent, pH level, solvent-water ratio and extraction time. In general, no significant differences in total phenolic recovery and ORAC values were observed when 90% methanol or methanol/acetone/water (45/45/10), both with 0.1% HCl, were used. By contrast, the 90% methanol solution with 0.1% HCl extracted the highest level of anthocyanins, whereas the solvent mixture extracted the highest level of flavanols. The purified extracts from the mashua genotypes presented total phenolics and ORAC contents within the ranges 14.4-18.7 mg gallic acid equivalents/g mashua dry matter (DM) and 221-359 mol of trolox equivalents/g mashua DM, respectively. The HPLC-DAD phenolic profiles obtained for the purified phenolic extracts for both mashua genotypes showed differences in proportions (% total area at 280 and 520 nm) depending on the solvents selected in this study. The high content of phenolic compounds and antioxidant activity found in the purified extracts for both mashua genotypes indicated that mashua extracts might be considered as a potential source of nutraceuticals in the future.

Enriquecimento de compostos fenólicos de folhas de Inga edulis por extração em fase sólida: quantificação de seus compostos majoritários e avaliação da capacidade antioxidante

Química Nova, 2010

Aç ai (Euterpe oleracea) fruit (EOF) are widely commercialized in the Brazilian Amazon. These fru... more Aç ai (Euterpe oleracea) fruit (EOF) are widely commercialized in the Brazilian Amazon. These fruit contain a high bacterial load and are transported on boards stowed inside or outside the holds of small boats. In this context, postharvest parameters were assessed under conditions that simulated these two methods of EOF transport: stowage in closed polystyrene boxes, simulating the inside of cargo holds, i.e., transport in a closed system; and open baskets, simulating transport in an open environment, i.e., transport in the prow or bow of the boat. EOF suffered spontaneous fermentation of alcoholic, acetic, and lactic types in the closed system, which is the most common type of transportation of this fruit. In the closed system, there was a predominance of lactic acid bacteria over acetic acid bacteria, with 82% and 95% of the initial content of d-glucose and d-fructose being consumed, respectively, after 27 h of experiment. The weight loss reached 1.7% and there was a logarithmic decrease of the major phenolic compounds of the fruit in the closed system, with losses of 78% of cyanidin-3-rutinoside, 88% of cyanidin-3-glucoside, 78% of homorientin, and 72% of orientin after 27 h, which was higher than in the open system (58%, 66%, 73% and 62%, respectively). Analyses on EOF stowed in a closed system indicated that the respiratory rate was characteristic of a non-climacteric fruit, i.e., it showed a logarithmic decay in the production of CO 2 (R 2 = 0.995; P < 0.05). Thus, transport in a closed system results in more drastic nutritional and functional changes on EOF than when transport is carried out in an open system, suggesting that transportation in continuous aerobic conditions and a short period of time between picking and processing are preferable.

Journal of the Science of Food and Agriculture, 2007

The total phenolics, anthocyanins, flavan 3-ols, carotenoids and antioxidant capacity of mashua (... more The total phenolics, anthocyanins, flavan 3-ols, carotenoids and antioxidant capacity of mashua (Tropaeolum tuberosum Ruiz & Pavón) tubers from 10 yellow and purple cultivars were determined at different maturity stages (5-7.5 months after planting) and sunning post-harvest storage periods (7-35 days). Both the antioxidant capacity (ORAC and ABTS assays) and the content of the bioactive compounds tested varied markedly between cultivars. Purple varieties reached the highest antioxidant capacity during tuber development (271-446 µmol Trolox equiv g −1 DM, ORAC assay). The kinetics of accumulation or disappearance of the bioactive compounds tested during maturation was dependent both on the cultivar and on the compound considered. For anthocyanins, there was a marked increase during maturation in all the purple cultivars. During the sunning postharvest storage, the changes in antioxidant capacity (ABTS assay) and content of the bioactive compounds tested also varied between cultivars. A marked decrease in anthocyanins was observed for the anthocyanin-containing cultivars. In general, the correlation between antioxidant capacity and the content of bioactive compounds varied markedly between cultivars. Antioxidant capacity in purple varieties correlated with total phenolics or flavan 3-ols while only in some yellow varieties antioxidant capacity correlated with total phenolics.

Kinetic Modeling of Anthocyanin Degradation and Microorganism Growth during Postharvest Storage of Açai Fruits ( Euterpe oleracea )

Journal of Food Science, 2012

The unavoidable damage of açai (Euterpe oleracea) fruits (AF) during picking leads to microbial c... more The unavoidable damage of açai (Euterpe oleracea) fruits (AF) during picking leads to microbial contamination and anthocyanin degradation, which prejudice the consumed fruit drink. Thirteen lots of AF (24 kg) from different municipal districts of the Pará State (Brazil) were monitored during a 75-h-long storage in the dark at 30 °C for microbial growth, and 7 lots for anthocyanin degradation. On arrival at the laboratory, anthocyanins presented a mean concentration of 828 mg kg(-1) fruits with a standard deviation of 323 mg kg(-1) fruits whereas mean microbial contamination was 2.64 10(6) CFU g(-1) of dry matter for total mesophilic bacteria, 1.98 10(3) MPN g(-1) DM for fecal coliforms, and 1.11 10(5) CFU g(-1) DM for moulds and yeasts. Kinetic growth of the microbes could be fitted to a quadratic equation with an unusual rapid growth during the 1st 24 h. The kinetics of anthocyanin degradation fitted a 1st-order equation. The mean velocity constant of the reaction (k(1)) was of 0.0137 h(-1) and the mean half-life (t(½)) of the anthocyanins was 50 h. These results indicate that the AF simultaneously suffer extensive anthocyanin degradation and explosive microbial growth during the postharvest period needing a special care.

Sigmoidal kinetics of anthocyanin accumulation during fruit ripening: A comparison between açai fruits (Euterpe oleracea) and other anthocyanin-rich fruits

Journal of Food Composition and Analysis, 2011

... such an accumulation profile is the sigmoidal model 54 (Woodward, 1972; Rogiers and Knowles, ... more ... such an accumulation profile is the sigmoidal model 54 (Woodward, 1972; Rogiers and Knowles, 1997; Fern ndez-L pez et al., 1999; Rivera-L pez ... (2009) investigated the accumulation of five anthocyanins during 157 ripening (25 33 days) of Prunus domestica ( Jojo , Valor , a ...

Spontaneous postharvest fermentation of açai (Euterpe oleracea) fruit

Postharvest Biology and Technology, 2013

Journal of the Science of Food and Agriculture, 2007

The total phenolics, anthocyanins, flavan 3-ols, carotenoids and antioxidant capacity of mashua (... more The total phenolics, anthocyanins, flavan 3-ols, carotenoids and antioxidant capacity of mashua (Tropaeolum tuberosum Ruiz & Pavón) tubers from 10 yellow and purple cultivars were determined at different maturity stages (5-7.5 months after planting) and sunning post-harvest storage periods (7-35 days). Both the antioxidant capacity (ORAC and ABTS assays) and the content of the bioactive compounds tested varied markedly between cultivars. Purple varieties reached the highest antioxidant capacity during tuber development (271-446 µmol Trolox equiv g −1 DM, ORAC assay). The kinetics of accumulation or disappearance of the bioactive compounds tested during maturation was dependent both on the cultivar and on the compound considered. For anthocyanins, there was a marked increase during maturation in all the purple cultivars. During the sunning postharvest storage, the changes in antioxidant capacity (ABTS assay) and content of the bioactive compounds tested also varied between cultivars. A marked decrease in anthocyanins was observed for the anthocyanin-containing cultivars. In general, the correlation between antioxidant capacity and the content of bioactive compounds varied markedly between cultivars. Antioxidant capacity in purple varieties correlated with total phenolics or flavan 3-ols while only in some yellow varieties antioxidant capacity correlated with total phenolics.

Tecnologias para Inovação nas cadeias Euterpe - Chapter 9

DRYING OF MURUCIZEIRO LEAVES (Byrsonima crassifolia) IN THIN LAYER AT DIFFERENT CONDITIONS

Byrsonima crassifolia, known in Brazil as murucizeiro, is a productive tree, present in all the A... more Byrsonima crassifolia, known in Brazil as murucizeiro, is a productive tree, present in all the Amazonian Region and in Central America. Its two main products, simultaneously and rationally exploitable are the fruits and the leaves, the last ones having a high antioxidant capacity. Due to the interest for their antioxidant capacity, the drying process of the murucizeiro leaves has been

Anticonvulsant properties of Euterpe oleracea in mice

Neurochemistry international, 2015

Açai (Euterpe oleracea Mart.), a highly consumed fruit in Amazon,is from a common palm with remar... more Açai (Euterpe oleracea Mart.), a highly consumed fruit in Amazon,is from a common palm with remarkable antioxidant properties. Because oxidative stress and seizures are intimately linked, this study investigated the potential neuroprotective and anticonvulsant effects of commercial clarified açai juice (EO). EO did not alter spontaneous locomotor activity. Four doses of EO were sufficient to increase latencies to both first myoclonic jerk and first generalized tonic-clonic seizure and significantly decrease the total duration of tonic-clonic seizures caused by pentylenetetrazol administration. Also, electrocortical alterations provoked by pentylenetetrazol were prevented, significantly decreasing amplitude of discharges and frequencies above 50 Hz. EO was also able to completely prevent lipid peroxidation in the cerebral cortex, showing a potent direct scavenging property. These results demonstrate for the first time that E. oleracea significantly protects against seizures and seizu...

Food Chemistry, 2015

The present study investigated the phenolic profiles of the pulp and peel of nine plantain cultiv... more The present study investigated the phenolic profiles of the pulp and peel of nine plantain cultivars and compared them to those of two dessert bananas of commercial interest (Grand Nain and Gros Michel), alongside a newly created hybrid, resistant to black sigatoka disease (F568). Identification and quantification of phenolic compounds were performed by means of HPLC-ESI-HR-MS and HPLC-DAD. Hydroxycinnamic acids, particularly ferulic acid-hexoside with 4.4-85.1 lg/g of dry weight, dominated in the plantain pulp and showed a large diversity among cultivars. Flavonol glycosides were predominant in plantain peels, rutin (242.2-618.7 lg/g of dry weight) being the most abundant. A principal component analysis on the whole data revealed that the phenolic profiles of the hybrid, the dessert bananas and the pure plantains differed from each other. Plantain pulps and peels appeared as good sources of phenolics, which could be involved in the health benefits associated with their current applications.

Separation and Purification Technology, 2007

Adsorption on macroporous resins was tested as a means of purifying the phenolic compounds from c... more Adsorption on macroporous resins was tested as a means of purifying the phenolic compounds from crude extracts of Inga edulis leaves. The experimental design methodology was used to optimise this process. The variables tested were the water proportion (50, 70 and 90%; v/v) in the hydroalcoholic extract solution, its pH value (2.0, 3.5 and 5.0) and the type of adsorbent used (XAD-7, XAD-16, EXA-90 and EXA-118). The time of contact was fixed at 2 h on the basis of kinetic studies. Statistically significant differences (p < 0.05) were obtained for the water proportion and the type of adsorbent, whereas the pH of the extract had no influence on the efficiency of adsorption. In all conditions, the XAD-7 resin gave the best results, reaching 239 mg equivalent phenolics per g resin , and XAD-16 the worst ones. Sorption isotherms were constructed for these two resins under optimal ethanol conditions and fitted well to the Freundlich and Langmuir models (R 2 > 0.95). The optimized results will be used in the design of fixed-bed adsorption systems for the purification and concentration of phenolic compounds from I. edulis leaves.

Separation and Purification Technology, 2007

The extraction of phenolic compounds from Inga edulis leaves was optimized by the simultaneous ma... more The extraction of phenolic compounds from Inga edulis leaves was optimized by the simultaneous maximization of the yield in total phenolics (TP), total flavanoids (TFA), and total flavonols (TFO), using the response surface methodology (RSM). A first set of experiments allowed identifying the temperature, the time of contact and the ethanol proportion in the extraction solution, as the main variables affecting the extraction efficiency. A rotatable central composite design consisting of 18 experimental runs with three replicates at the centre point was then applied and a second-order polynomial model was used to describe the experimental data regarding TP, TFA and TFO. The experimental results fitted well to the model and more than 85% of the variability was explained. TP, TFA and TFO showed different patterns of extractability, with significant variation in the linear, quadratic, and interaction effects of the independent variables. The optimized conditions were 86.8% ethanol, 58.2 • C and a time of contact of 46.8 min. The corresponding predicted values were 134.6 mg gallic acid equiv./g dry matter (DM), 26.0 mg catechin equiv./g DM and 13.8 mg rutin equiv./g DM, for TP, TFA and TFO, respectively. The experimental values agreed with those predicted within a 95% confidence interval, thus indicating the suitability of RSM in optimizing the extraction of phenolics from I. edulis.

Antioxidant properties of açai (Euterpe oleracea) in human plasma

Açai is a very popular polyphenol-rich fruit juice in the Amazonian region. In order to evaluate ... more Açai is a very popular polyphenol-rich fruit juice in the Amazonian region. In order to evaluate its effects on blood oxidative level, 350 ml açai juice were added to the daily diet of 30 male volunteers (aged 41±8.9 years) during 28 days. Fasting blood samples were obtained by vein puncture on day 0, 10, 20 and 28 and LDLs were

Immunomodulatory action of Copaifera spp oleoresins on cytokine production by human monocytes

Biomedicine & Pharmacotherapy, 2015

Copaifera spp oleoresins have been used in folk medicine for centuries; nevertheless, its immunom... more Copaifera spp oleoresins have been used in folk medicine for centuries; nevertheless, its immunomodulatory action has not been investigated. Thus, the goal of this study was to characterize different oleoresins and to verify their action on human monocytes regarding pro- and anti-inflammatory cytokine production (TNF-α and IL-10, respectively). The chemical composition of Brazilian Copaifera reticulata, Copaifera duckey and Copaifera multijuga oleoresins was analyzed by HPLC-MS. Cell viability was assessed by MTT method after incubation of cells with Copaifera spp. Noncytotoxic concentrations of oleoresins were incubated with human monocytes from healthy donors, and cytokine production was determined by ELISA. HPLC-MS analysis for terpenes allowed the identification of six diterpene acids and one sesquiterpene acid. Oleoresins exerted no cytotoxic effects on human monocytes. All oleoresins had a similar profile: LPS-induced TNF-α production was maintained by oleoresins, while a significant inhibitory action on IL-10 production was seen. Copaifera oleoresins seemed to exert an activator profile on human monocytes without affecting cell viability. Such effect may be due to the presence of either diterpene or sesquiterpene acids; however, further studies are necessary to determine the involvement of such compounds in Copaifera immunomodulatory effects.

Journal of The Brazilian Chemical Society, 2007

Um extrato metanol-água das folhas de Inga edulis foi fracionado para identificar os compostos po... more Um extrato metanol-água das folhas de Inga edulis foi fracionado para identificar os compostos polifenólicos. Os compostos identificados foram o acido gálico, a catequina, a epicatequina, a miricetina-3-ramnopiranosídeo, a quercetina-3-glucopiranosídeo e a quercetina-3-ramnopiranosídeo. A capacidade antioxidante do extrato e dos polifenóis puros foi medida pelo teste ORAC e comparada com o teor em fenólicos totais (TP). O extrato bruto seco apresentou valores de ORAC (11.16 mmol TE per g) e TP (496.5 mg GAE per g) muito altos. Os compostos identificados foram responsáveis, respectivamente, por 9.53 % e 12.10 % dos valores ORAC e de TP do extrato de folhas de Inga edulis.

Food Chemistry, 2008

Many plants used in Amazonian folk medicine present a high antioxidant activity. In this study, t... more Many plants used in Amazonian folk medicine present a high antioxidant activity. In this study, the antioxidant activities of four largely used plants, namely Byrsonima crassifolia, Davilla kunthii, Davilla rugosa and Inga edulis, were evaluated, using methanolic extracts of their leaves, fruits and bark and several different in vitro tests, based either on the capacity to scavenge free radicals (ORAC,

Nutritional composition and antioxidant properties of the sim fruit (Rhodomyrtus tomentosa)

Food Chemistry, 2015

In this study, detailed chemical properties of sim (Rhodomyrtus tomentosa (Ait.) Hassk.) fruit in... more In this study, detailed chemical properties of sim (Rhodomyrtus tomentosa (Ait.) Hassk.) fruit including nutritional composition, phenolic content and antioxidant capacity were determined for the first time. A 150g serving of sim fruit contained high levels of dietary fibre (69.94-87.43% of Recommended Daily Intake (RDI)), α-tocopherol (38.90-51.87% RDI), manganese (&amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;amp;gt;100% RDI), and copper (44.44% RDI) but low levels of protein (2.63% RDI), lipid (1.59-3.5% RDI), and sugars (5.65% RDI). The predominant fatty acid in the sim fruit sample was linoleic acid (75.36% of total fatty acids). Interestingly, total phenolics (49.21±0.35mg gallic acid equivalent (GAE)/g dry weight (DW)) were particularly high and resulted in a high antioxidant capacity (431.17±14.56μmol Trolox equivalent (TE)/g DW). These results, together with our recent discovery of high amount of piceatannol, a stilbene with potent biological activities, highlight the potential of sim, an under-utilised plant species from South-East Asia, as a new source of health-promoting compounds including dietary fibres, essential fatty acids, and phenolic compounds.

Equilibria, Kinetics, and Mechanisms for the Adsorption of Four Classes of Phenolic Compounds onto Synthetic Resins

Separation Science and Technology, 2010

The adsorption of five phenolic compounds of four different classes from aqueous batch solutions ... more The adsorption of five phenolic compounds of four different classes from aqueous batch solutions onto four styrene-divinylbenzene and acrylic resins (EXA 90, EXA 118, XAD 7, and XAD 16) was investigated regarding their equilibria, kinetics and surface-energy heterogeneity, and mechanisms of adsorption. The experimental equilibrium data were very well fitted to Langmuir and Freundlich models (R &amp;amp;amp;gt; 0.900). Three kinetic models

Separation and Purification Technology, 2007

Optimal conditions for the extraction of phenolic compounds, anthocyanins, flavanols and ORAC ant... more Optimal conditions for the extraction of phenolic compounds, anthocyanins, flavanols and ORAC antioxidant activity for two mashua genotypes were determined using the conventional liquid-solid method. Phenolic contents and antioxidant activity were affected by the type of solvent, pH level, solvent-water ratio and extraction time. In general, no significant differences in total phenolic recovery and ORAC values were observed when 90% methanol or methanol/acetone/water (45/45/10), both with 0.1% HCl, were used. By contrast, the 90% methanol solution with 0.1% HCl extracted the highest level of anthocyanins, whereas the solvent mixture extracted the highest level of flavanols. The purified extracts from the mashua genotypes presented total phenolics and ORAC contents within the ranges 14.4-18.7 mg gallic acid equivalents/g mashua dry matter (DM) and 221-359 mol of trolox equivalents/g mashua DM, respectively. The HPLC-DAD phenolic profiles obtained for the purified phenolic extracts for both mashua genotypes showed differences in proportions (% total area at 280 and 520 nm) depending on the solvents selected in this study. The high content of phenolic compounds and antioxidant activity found in the purified extracts for both mashua genotypes indicated that mashua extracts might be considered as a potential source of nutraceuticals in the future.

Enriquecimento de compostos fenólicos de folhas de Inga edulis por extração em fase sólida: quantificação de seus compostos majoritários e avaliação da capacidade antioxidante

Química Nova, 2010

Aç ai (Euterpe oleracea) fruit (EOF) are widely commercialized in the Brazilian Amazon. These fru... more Aç ai (Euterpe oleracea) fruit (EOF) are widely commercialized in the Brazilian Amazon. These fruit contain a high bacterial load and are transported on boards stowed inside or outside the holds of small boats. In this context, postharvest parameters were assessed under conditions that simulated these two methods of EOF transport: stowage in closed polystyrene boxes, simulating the inside of cargo holds, i.e., transport in a closed system; and open baskets, simulating transport in an open environment, i.e., transport in the prow or bow of the boat. EOF suffered spontaneous fermentation of alcoholic, acetic, and lactic types in the closed system, which is the most common type of transportation of this fruit. In the closed system, there was a predominance of lactic acid bacteria over acetic acid bacteria, with 82% and 95% of the initial content of d-glucose and d-fructose being consumed, respectively, after 27 h of experiment. The weight loss reached 1.7% and there was a logarithmic decrease of the major phenolic compounds of the fruit in the closed system, with losses of 78% of cyanidin-3-rutinoside, 88% of cyanidin-3-glucoside, 78% of homorientin, and 72% of orientin after 27 h, which was higher than in the open system (58%, 66%, 73% and 62%, respectively). Analyses on EOF stowed in a closed system indicated that the respiratory rate was characteristic of a non-climacteric fruit, i.e., it showed a logarithmic decay in the production of CO 2 (R 2 = 0.995; P < 0.05). Thus, transport in a closed system results in more drastic nutritional and functional changes on EOF than when transport is carried out in an open system, suggesting that transportation in continuous aerobic conditions and a short period of time between picking and processing are preferable.

Journal of the Science of Food and Agriculture, 2007

The total phenolics, anthocyanins, flavan 3-ols, carotenoids and antioxidant capacity of mashua (... more The total phenolics, anthocyanins, flavan 3-ols, carotenoids and antioxidant capacity of mashua (Tropaeolum tuberosum Ruiz & Pavón) tubers from 10 yellow and purple cultivars were determined at different maturity stages (5-7.5 months after planting) and sunning post-harvest storage periods (7-35 days). Both the antioxidant capacity (ORAC and ABTS assays) and the content of the bioactive compounds tested varied markedly between cultivars. Purple varieties reached the highest antioxidant capacity during tuber development (271-446 µmol Trolox equiv g −1 DM, ORAC assay). The kinetics of accumulation or disappearance of the bioactive compounds tested during maturation was dependent both on the cultivar and on the compound considered. For anthocyanins, there was a marked increase during maturation in all the purple cultivars. During the sunning postharvest storage, the changes in antioxidant capacity (ABTS assay) and content of the bioactive compounds tested also varied between cultivars. A marked decrease in anthocyanins was observed for the anthocyanin-containing cultivars. In general, the correlation between antioxidant capacity and the content of bioactive compounds varied markedly between cultivars. Antioxidant capacity in purple varieties correlated with total phenolics or flavan 3-ols while only in some yellow varieties antioxidant capacity correlated with total phenolics.

Kinetic Modeling of Anthocyanin Degradation and Microorganism Growth during Postharvest Storage of Açai Fruits ( Euterpe oleracea )

Journal of Food Science, 2012

The unavoidable damage of açai (Euterpe oleracea) fruits (AF) during picking leads to microbial c... more The unavoidable damage of açai (Euterpe oleracea) fruits (AF) during picking leads to microbial contamination and anthocyanin degradation, which prejudice the consumed fruit drink. Thirteen lots of AF (24 kg) from different municipal districts of the Pará State (Brazil) were monitored during a 75-h-long storage in the dark at 30 °C for microbial growth, and 7 lots for anthocyanin degradation. On arrival at the laboratory, anthocyanins presented a mean concentration of 828 mg kg(-1) fruits with a standard deviation of 323 mg kg(-1) fruits whereas mean microbial contamination was 2.64 10(6) CFU g(-1) of dry matter for total mesophilic bacteria, 1.98 10(3) MPN g(-1) DM for fecal coliforms, and 1.11 10(5) CFU g(-1) DM for moulds and yeasts. Kinetic growth of the microbes could be fitted to a quadratic equation with an unusual rapid growth during the 1st 24 h. The kinetics of anthocyanin degradation fitted a 1st-order equation. The mean velocity constant of the reaction (k(1)) was of 0.0137 h(-1) and the mean half-life (t(½)) of the anthocyanins was 50 h. These results indicate that the AF simultaneously suffer extensive anthocyanin degradation and explosive microbial growth during the postharvest period needing a special care.

Sigmoidal kinetics of anthocyanin accumulation during fruit ripening: A comparison between açai fruits (Euterpe oleracea) and other anthocyanin-rich fruits

Journal of Food Composition and Analysis, 2011

... such an accumulation profile is the sigmoidal model 54 (Woodward, 1972; Rogiers and Knowles, ... more ... such an accumulation profile is the sigmoidal model 54 (Woodward, 1972; Rogiers and Knowles, 1997; Fern ndez-L pez et al., 1999; Rivera-L pez ... (2009) investigated the accumulation of five anthocyanins during 157 ripening (25 33 days) of Prunus domestica ( Jojo , Valor , a ...