Rogelio Alonso | Universidad Nacional Autónoma de México (original) (raw)

Papers by Rogelio Alonso

The objective of this study was to identify Pelibuey sheep resistant to H. contortus. The resista... more The objective of this study was to identify Pelibuey sheep resistant to H. contortus. The resistance criterion used was fecal egg counts during an experimental infection of 42 days. Fifty-three Pelibuey lambs of 5 months of age (22 males and 31 females), free of nematodes, were infected with 3,000 larvae of H. contortus. Lambs were monitored every week for fecal egg counts (FEC), hematocrit value (HV), plasma protein level (PP), eosinophil counts (EC) and total weight gain (TWG). On day 42 post-infection, lambs were drenched and then evaluated every 28 days for a year under natural infection conditions. The degree of resistance was determined using the average of the last four egg counts during the experimental infection. Data was normalized by a log10 transformation. Statistical analysis of the data was carried out through an analysis of variance. The classification criterion used was the transformed mean minus one standard deviation (criteria equivalent in actual count=1,200 egg c...

Journal of Animal and Veterinary Advances

In this study a 2x2 factorial arrangement was used to study the effect of sex and age on serum in... more In this study a 2x2 factorial arrangement was used to study the effect of sex and age on serum insulin levels in 24 growing Mexican Cuino pigs between 3 and 6 months old. There were no significant differences (p-1 of fasting serum insulin levels (p< 0.001) at 3 and 6 months old, respectively. Pearson correlation matrix revealed significant differences (p< 0.05) among age, live weigth and fasting insulin levels in the examined period of life in growing Mexican Cuino pigs. According to the present evaluation, hiperinsulinemia should be present in Mexican Cuino pigs, as it has been found in either obese conventional genotypes of pigs or genetically manipulated to be small and obese animals. This effect was accentuated as pigs aged up to 6 months life. Sex appeared to have no influence on serum status of Mexican Cuino pig insulin.

Canadian journal of veterinary research = Revue canadienne de recherche vétérinaire, 2007

Osteochondrodysplasias are caused by abnormal development and growth of cartilage and bone. These... more Osteochondrodysplasias are caused by abnormal development and growth of cartilage and bone. These abnormalities have been reported in both humans and animals with dwarfism. The basset hound is considered a breed with a disproportionate prevalence of dwarfism, the cause of which is unknown. To determine the type of osteochondrodysplasia in this breed, we analyzed histologically the growth plates from the long bones of a basset hound and a Doberman pinscher, both 2 mo old. Tissue was fixed in 4% paraformaldehyde, embedded in paraffin, sectioned at 5 microm, stained with hematoxylin and eosin, and analyzed by light microscopy. Our results suggest that by this method the basset hound can be defined only as a breed having osteochondrodysplasia due to a primary cartilage problem in the growth plate.

Journal of Biological Sciences, 2009

… de Producción Porcina …, 2007

El gen receptor de los estrógenos (ESR, estrogen receptor gene)) fue estudiado como gen candidato... more El gen receptor de los estrógenos (ESR, estrogen receptor gene)) fue estudiado como gen candidato para características reproductivas en cerdas de la raza comercial Yorkshire x Landrace. Un total de 300 marranas tipificadas genéticamente para ESR fueron analizadas para determinar la relación de este locus con el número de lechones nacidos totales (LNT), número de lechones nacidos vivos (LNV), número de lechones destetados (LD), peso de la camada al nacimiento (PNAC), peso de la camada al destete (PAJ21) y valor reproductivo de la cerda (VRDC). Los datos se analizaron por grupos de alta y baja producción respectivamente (105<VRDC<95) donde VRDC expresa valor reproductivo de la descendencia de la cerda. En ambos grupos, n =150. Se encontraron diferencias significativas (P<0.01) para la frecuencia del alelo B, que fue más alta en el grupo de alta producción, al igual que las frecuencias del genotipo AB; en ambos grupos no se detectaron animales homocigotos para el alelo B. El ESR estuvo asociado significativamente (P<0.001) con las variables LNV, LD, PAJ21 y VRDC, y en la comparación de medias resultó que el mejor genotipo fue el AB con ventajas de +0.43, +0.81, +8.82 y +11.07 unidades para estos rasgos en ese orden, respectivamente, cuando se comparó con el genotipo AA. No hubo diferencias significativas de los genotipos AA y AB dentro de los grupos de producción formados. Se puede concluir que los genotipos AB, con una copia del alelo favorable (B), se relacionan con características reproductivas de las cerdas, independientemente del nivel de producción al cual pertenecen mejorando sustancialmente el número de lechones nacidos vivos, número y peso de lechones destetados y el valor reproductivo de la progenie de la cerda. Palabras claves: cerdas, gen receptor de estrógenos, reproducción Título corto: Gen receptor de estrógenos y capacidad reproductiva en cerdas INFLUENCE OF THE ESTROGEN RECEPTOR GENE (ESR) ON THE REPRODUCTIVE CAPACITY OF SOWS FROM A COMMERCIAL HERD SUMMARY The estrogen receptor gene was studied as a candidate gene for reproductive characteristics in sows of the commercial Yorkshire x Landrace cross. A total of 300 female pigs were generically typified for ESR and analyzed for determining the relationship amongst this locus and total born piglets (TBP), piglets born alive (PBA), weaned piglets (WP), litter weight at birth (LWB), litter weight at weaning (LWW) and sow reproductive value (SRV), expressing progeny characteristics of sows. Data were analyzed according to groups of high and low productivity respectively (105<SRV<95). In both groups, n = 150. There were significant differences (P<0.01) for allele B frequency which was highest in the group of high productivity, in line with frequency for the AB genotype; homozygote animals were not detected for allele B in both groups. The ESR was significantly (P<0.001) associated to TBP, WP, LWW and SRV where AB was better than AA genotype when means were compared, with advantages of +0.43, +0.81, +8.82 and +11.07 units in this same order, respectively. There were no significant differences between AA and AB genotypes within both groups of production. It could be concluded that animals from the AB genotype, therefore containing a copy of the favorable allele (B), are related to reproductive characteristics of sows, with independence to the considered production level of sows, improving substantially the number of born alive piglets, the number and weight of weaned piglets and the reproductive value of progeny of sows.

Rev. Cient.( …, 2006

Se estudiaron genes candidatos para tamaño de la camada en 300 hembras porcinas Yorkshire-Landrac... more Se estudiaron genes candidatos para tamaño de la camada en 300 hembras porcinas Yorkshire-Landrace; ESR, PRLR, RBP4 y FUT1. Las hembras fueron agrupadas en dos niveles de producción (NP): nivel alto (NA) y nivel bajo (NB). Utilizando Ji cuadrado se analizaron las frecuencias génicas y genotípicas. Empleando análisis de varianza con un modelo de efectos mixtos, para lechones nacidos totales (LNT), nacidos vivos (LNV), peso de la camada al nacimiento (PNAC) y destete (PAJ21), lechones destetados (LD) y valor de cría de la progenie de la cerda (BVSP), se compararon las medias con contrastes ortogonales. Las hembras con alta productividad se asociaron con una mayor frecuencia del alelo B del gen ESR (P < 0,05). Las diferencias fueron de 0,4 LNV, 0,3 LD, 2,9 Kg de PAJ21 y 8,6 puntos de BVSP a favor del genotipo AB del gen ESR (P < 0,05) sin considerar el NP, no se detectaron animales homocigotos BB. Las frecuencias génicas y genotípicas del gen PRLR no se relacionaron con el NP (P > 0,05), no hubo diferencias (P > 0,05) entre los genotipos AA, AB y BB sin considerar el NP ni dentro del mismo NP. En el gen RBP4 la frecuencia del alelo A y del genotipo AA fue más alta en hembras con NA (P < 0,05), no se detectaron animales con genotipo BB. Las hembras con genotipos AA tuvieron más 0,5 LNT; 0,5 LNV; 0,6 Kg de PNAC; 2,6 Kg de PAJ21 y 3,2 puntos de BVSP que el genotipo AB (P < 0,05), sin considerar el NP. La frecuencia del alelo G y del genotipo GG del gen FUT1 fue mayor en el nivel de productividad alto (P < 0,05). El genotipo GG fue superior al genotipo AG con más 0,6 LNV; 0,8 Kg de PNAC; 3 Kg de PAJ21 y 3,9 puntos de BVSP (P < 0,05) sin considerar el NP.

Genetics and Molecular Biology, 2008

The polymorphism of locus BoLA-DRB3.2 of the Major Histocompatibility Complex was evaluated in tw... more The polymorphism of locus BoLA-DRB3.2 of the Major Histocompatibility Complex was evaluated in two northern Mexican Creole cattle populations, Chihuahua (n = 47) and Tamaulipas (n = 51). The BoLA-DRB3.2 locus was typed by amplification and digestion with restriction endonuclease enzymes (PCR-RFLP). Fifty-two alleles were detected (28 previously reported and 24 new ones). In the Chihuahua population, 18 alleles and 5.5 effective alleles were found, while in the Tamaulipas population there were 34 and 10.8, respectively. The allele frequencies ranged from 0.011 to 0.383 in Chihuahua and from 0.010 to 0.206 in Tamaulipas. The frequencies of the new alleles in both cattle populations were low (0.010 to 0.053). The expected heterozygosity was 0.827 and 0.916, respectively, for the Chihuahua and Tamaulipas populations. Both populations presented a heterozygote deficit: [Chihuahua F IS = 0.1 (p = 0.019) and Tamaulipas F IS = 0.317 (p < 0.001)]. In conclusion, this study showed that the Mexican Creole cattle have many low-frequency alleles, several of which are exclusive to these populations. Genetic distances obtained show that the Mexican Creole cattle population is composed of independent populations, far apart from other South American Creole populations.

Journal of Cell Biology, 1989

The giardins are a group of 29-38-kD proteins in the ventral disk of the protozoan parasite Giard... more The giardins are a group of 29-38-kD proteins in the ventral disk of the protozoan parasite Giardia lamblia. The disk attaches the parasite to the host's intestinal epithelium and is composed of parallel, coiled microtubules that are adjacent to the ventral plasma membrane and from which processes called microribbons extend into the cytoplasm; the microribbons are connected by crossbridges. G. lamblia cytoskeletons, consisting of disks and attached flagella, were isolated and used to show that the 29-38-kD proteins separate into five bands by one-dimensional electrophoresis and into 23 species by two-dimensional analysis. Rabbit antibodies raised against a 33-kD protein band, purified by one-dimensional gel electrophoresis and shown to contain three proteins by two-dimensional electrophoresis, recognized 17 proteins by two-dimensional immunoblot analysis. By immunofluorescence these antibodies reacted with the ventral disk but not with the flagella in isolated cytoskeletons. Ele...

Revista Mexicana de Ciencias Pecuarias, 2009

In order to learn about the mitochondrial genetic origin of Mexican creole sheep populations, blo... more In order to learn about the mitochondrial genetic origin of Mexican creole sheep populations, blood samples from 106 of these animals were collected in the Mexican States of Chiapas, Puebla, Morelos, Hidalgo and Veracruz. Samples were collected from creole-Suffolk admixed (n=23), Pelibuey (n=15) and Black Belly (n=6) sheep. Cytochrome C oxidase subunit I gene (COXI) is located in the mitochondrion. This fragment has a polymorphic site for the HinfI enzyme in the 5562-5566 position that allows for differentiating between Asian and European genotypes of Ovis aries. A polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis was used. A 1053 base pair (bp) fragment of the COX 1 gene was amplified then digested with the HinfI restriction enzyme. All samples from creole sheep and creole crosses revealed the genotype B of the COX 1 gene. Nevertheless, only one Black Belly animal had genotype A. Therefore, local Mexican sheep (creole, Chiapas and Pelibuey breed...

Electronic Journal of Biotechnology, 2008

Electronic Journal of Biotechnology ISSN: 0717-3458 Vol.11 No.3, Issue of July 15, 2008 © 2008 by... more Electronic Journal of Biotechnology ISSN: 0717-3458 Vol.11 No.3, Issue of July 15, 2008 © 2008 by Pontificia Universidad Catolica de Valparaiso -- Chile Received November 19, 2007 / Accepted March 14, 2008

Revista Mexicana de Ciencias Pecuarias, 2014

El estudio genético en razas de ovejas que naturalmente presentan altas tasas de ovulación (TO) y... more El estudio genético en razas de ovejas que naturalmente presentan altas tasas de ovulación (TO) y de prolificidad, ha permitido detectar la participación de varios genes. Entre ellos están los relacionados a lasuperfamilia del factor de crecimiento transformante B (BMPRIB, GDF9 y BMP-15), así como de otros genes con efecto mayor, tales como el “distal-less homeobox 3” (FecL), el receptor de estrógenos (ESR), el receptor de prolactina (PRLR) y el de las inhibinas (INHA y INHB). Sin embargo, las ovejas homocigotas para la mayoría de las variantes alélicas en BMP-15 o GDF9 son estériles, por lo que es importante el entendimiento de las bases genéticas y moleculares de estos polimorfismos, para su uso en programas racionales de mejoramiento genético con énfasis en la prolificidad. El polimorfismo en el gen de la melatonina (MTNR1) se ha asociado con la no estacionalidad reproductiva en ovejas. La selección en varias razas de ovejas para estas características ha reducido significativamen...

Veterinaria México OA, 2016

The epidemiological and clinicopathological features of canine mammary tumors may provide valuabl... more The epidemiological and clinicopathological features of canine mammary tumors may provide valuable information to facilitate analysis of the behavior of the disease and represent a potential tool for the study of breast cancer in women. The aim of this study was to associate the histological features of canine mammary tumors with potential risk factors and survival. One hundred and seventy-eight mammary tumors were collected from 80 female dogs. The statistical analyses consisted of a series of univariate studies and frequencies of the different study variables, such as a bivariate analysis with the Chi squared test (χ 2), a relative risk and Kaplan Meier survival analysis, and a multiple correspondence analysis was used to correlate the tumor's biological behavior with the dogs' breed. Most patients were older than 8 years and had at least one malignant tumor, which was usually solitary and measured more than 6 cm; these patients had poor survival. The most frequent tumors were a complex adenoma, benign mixed tumor, carcinoma complex and mixed type carcinoma. The most commonly affected breeds were Poodle, Cocker Spaniel and Dachshund, and the breeds at the highest risk of tumor development were Cocker Spaniel, Labrador Retriever and German Shepherd. Overall, the data indicated that mammary tumors in dogs mainly affected older females with malignant tumors and that there were high mortality and short-term survival rates. However, the most commonly affected breeds were not necessarily the most susceptible. Our data do not support the hypothesis of an increased risk of canine mammary tumors in nulliparous female dogs.

Journal of Animal and Veterinary Advances

ABSTRACT

Con el objetivo de determinar la inocuidad bacteriológica de los quesos frescos y semimadurados q... more Con el objetivo de determinar la inocuidad bacteriológica de los quesos frescos y semimadurados que se venden en algunos ¿mercados sobre ruedas¿ en la ciudad de México, se realizó la detección simultánea de Salmonella spp y de Listeria monocytogenes, mediante la técnica de reacción en cadena de la polimerasa (PCR), así como con los métodos bacteriológicos convencionales, según la normatividad correspondiente para cada microorganismo; es decir, la NOM-114-SSA1-1994 mexicana, que constituye un método para la determinación de Salmonella en alimentos; de igual manera la NOM-143-SSA1-1995 mexicana, que representa un método de prueba microbiológica para alimentos y determinación de L. monocytogenes. Se analizaron 120 muestras seleccionadas al azar, provenientes de cuatro ¿mercados sobre ruedas¿ de una zona del sur de la ciudad de México. La metodología propuesta para la PCR múltiple se basó en la amplifi cación simultánea de los genes InvA e Iap procedentes de los genomas de Salmonella sp...

Journal of Dairy Research, 2012

The objective of this study was to evaluate the effects of the CSN1S1 locus polymorphism on 305-d... more The objective of this study was to evaluate the effects of the CSN1S1 locus polymorphism on 305-d records of milk, fat, protein, lactose and total solids yields, fat, protein, lactose and total solids contents in Mexican dairy goats. A total of 514 lactation records belonging to Alpine (n=60), Saanen (n=105) and Toggenburg (n=74) goats, born from 2003 to 2006 in three herds were used. Discrimination between alleles E, F, N, A* (CSN1S1 A, G, H, I, O1 and O2) and B* (CSN1S1 B1, B2, B3, B4, C and L) were made by amplification of fragments of the gene CSN1S1 and digestion with the restriction endonuclease XmnI. In order to estimate additive and dominance effects, data sets including (1) all genotypes, and (2) only homozygote genotypes, were analysed using linear mixed models. The allele A*, had significant additive effects for protein content (0·21±0·07%;P=0·002) and total solids content (0·66±0·23%;P=0·005) when compared with allele F. An unfavourable additive effect of allele A* on mi...

Journal of Animal Breeding and Genetics, 2008

The objective of this study was to evaluate the genetic structure of Mexican Criollo cattle popul... more The objective of this study was to evaluate the genetic structure of Mexican Criollo cattle populations using microsatellite genetic markers. DNA samples were collected from 168 animals from four Mexican Criollo cattle populations, geographically isolated in remote areas of Sierra Madre Occidental (West Highlands). Also were included samples from two breeds with Iberian origin: the fighting bull (n = 24) and the milking central American Criollo (n = 24) and one Asiatic breed: Guzerat (n = 32). Genetic analysis consisted of the estimation of the genetic diversity in each population by the allele number and the average expected heterozygosity found in nine microsatellite loci. Furthermore, genetic relationships among the populations were defined by their genetic distances. Our data shows that Mexican cattle populations have a relatively high level of genetic diversity based either on the mean number of alleles (10.2-13.6) and on the expected heterozygosity (0.71-0.85). The degree of observed homozygosity within the Criollo populations was remarkable and probably caused by inbreeding (reduced effective population size) possibly due to reproductive structure within populations. Our data shows that considerable genetic differentiation has been occurred among the Criollo cattle populations in different regions of Mexico.

Genetics and Molecular Biology, 2006

Bovine tuberculosis, caused by Mycobacterium bovis, is a disease of socioeconomic and public heal... more Bovine tuberculosis, caused by Mycobacterium bovis, is a disease of socioeconomic and public health importance and of significance to international trade regulation. Allelic variants of several genes have been implicated in the genetic susceptibility to tuberculosis in some human populations, but little is known in cattle. We surveyed 34 European, 18 Asian, 20 Creole and 23 hybrid bovines for polymorphisms of the bovine solute carrier family 11 a1(Slc11a1) gene, formerly known as natural resistance associated macrophage protein (Nramp1), gene by typing the cattle using two microsatellite loci closely linked to this gene. The microsatellites used were 311-22, located at the 3' untranslated region (3' UTR) of the Slc11a1 gene, and ARO28 situated about 0.6 cM upstream of the same gene Based on allele size in base pairs (bp) we determined five 311-223 locus variants (221, 223, 225, 227 and 229 bp) and 12 ARO28 loci. There was marked diversity and a very high level of heterozygosity in most of the cattle surveyed except the Europeans bovines and especially Holsteins in relation to the 3' UTR microsatellite locus.

Electronic Journal of Biotechnology, 2008

CyTA - Journal of Food, 2009

El objetivo de este trabajo fue desarrollar una técnica de reacción en cadena de la polimerasa (P... more El objetivo de este trabajo fue desarrollar una técnica de reacción en cadena de la polimerasa (PCR) múltiple para la detección simultánea de Salmonella spp. y Escherichia coli O157:H7 en canales de bovino. Para obtener los productos de PCR, se amplifican los genes slt-I, ...

The objective of this study was to identify Pelibuey sheep resistant to H. contortus. The resista... more The objective of this study was to identify Pelibuey sheep resistant to H. contortus. The resistance criterion used was fecal egg counts during an experimental infection of 42 days. Fifty-three Pelibuey lambs of 5 months of age (22 males and 31 females), free of nematodes, were infected with 3,000 larvae of H. contortus. Lambs were monitored every week for fecal egg counts (FEC), hematocrit value (HV), plasma protein level (PP), eosinophil counts (EC) and total weight gain (TWG). On day 42 post-infection, lambs were drenched and then evaluated every 28 days for a year under natural infection conditions. The degree of resistance was determined using the average of the last four egg counts during the experimental infection. Data was normalized by a log10 transformation. Statistical analysis of the data was carried out through an analysis of variance. The classification criterion used was the transformed mean minus one standard deviation (criteria equivalent in actual count=1,200 egg c...

Journal of Animal and Veterinary Advances

In this study a 2x2 factorial arrangement was used to study the effect of sex and age on serum in... more In this study a 2x2 factorial arrangement was used to study the effect of sex and age on serum insulin levels in 24 growing Mexican Cuino pigs between 3 and 6 months old. There were no significant differences (p-1 of fasting serum insulin levels (p< 0.001) at 3 and 6 months old, respectively. Pearson correlation matrix revealed significant differences (p< 0.05) among age, live weigth and fasting insulin levels in the examined period of life in growing Mexican Cuino pigs. According to the present evaluation, hiperinsulinemia should be present in Mexican Cuino pigs, as it has been found in either obese conventional genotypes of pigs or genetically manipulated to be small and obese animals. This effect was accentuated as pigs aged up to 6 months life. Sex appeared to have no influence on serum status of Mexican Cuino pig insulin.

Canadian journal of veterinary research = Revue canadienne de recherche vétérinaire, 2007

Osteochondrodysplasias are caused by abnormal development and growth of cartilage and bone. These... more Osteochondrodysplasias are caused by abnormal development and growth of cartilage and bone. These abnormalities have been reported in both humans and animals with dwarfism. The basset hound is considered a breed with a disproportionate prevalence of dwarfism, the cause of which is unknown. To determine the type of osteochondrodysplasia in this breed, we analyzed histologically the growth plates from the long bones of a basset hound and a Doberman pinscher, both 2 mo old. Tissue was fixed in 4% paraformaldehyde, embedded in paraffin, sectioned at 5 microm, stained with hematoxylin and eosin, and analyzed by light microscopy. Our results suggest that by this method the basset hound can be defined only as a breed having osteochondrodysplasia due to a primary cartilage problem in the growth plate.

Journal of Biological Sciences, 2009

… de Producción Porcina …, 2007

El gen receptor de los estrógenos (ESR, estrogen receptor gene)) fue estudiado como gen candidato... more El gen receptor de los estrógenos (ESR, estrogen receptor gene)) fue estudiado como gen candidato para características reproductivas en cerdas de la raza comercial Yorkshire x Landrace. Un total de 300 marranas tipificadas genéticamente para ESR fueron analizadas para determinar la relación de este locus con el número de lechones nacidos totales (LNT), número de lechones nacidos vivos (LNV), número de lechones destetados (LD), peso de la camada al nacimiento (PNAC), peso de la camada al destete (PAJ21) y valor reproductivo de la cerda (VRDC). Los datos se analizaron por grupos de alta y baja producción respectivamente (105<VRDC<95) donde VRDC expresa valor reproductivo de la descendencia de la cerda. En ambos grupos, n =150. Se encontraron diferencias significativas (P<0.01) para la frecuencia del alelo B, que fue más alta en el grupo de alta producción, al igual que las frecuencias del genotipo AB; en ambos grupos no se detectaron animales homocigotos para el alelo B. El ESR estuvo asociado significativamente (P<0.001) con las variables LNV, LD, PAJ21 y VRDC, y en la comparación de medias resultó que el mejor genotipo fue el AB con ventajas de +0.43, +0.81, +8.82 y +11.07 unidades para estos rasgos en ese orden, respectivamente, cuando se comparó con el genotipo AA. No hubo diferencias significativas de los genotipos AA y AB dentro de los grupos de producción formados. Se puede concluir que los genotipos AB, con una copia del alelo favorable (B), se relacionan con características reproductivas de las cerdas, independientemente del nivel de producción al cual pertenecen mejorando sustancialmente el número de lechones nacidos vivos, número y peso de lechones destetados y el valor reproductivo de la progenie de la cerda. Palabras claves: cerdas, gen receptor de estrógenos, reproducción Título corto: Gen receptor de estrógenos y capacidad reproductiva en cerdas INFLUENCE OF THE ESTROGEN RECEPTOR GENE (ESR) ON THE REPRODUCTIVE CAPACITY OF SOWS FROM A COMMERCIAL HERD SUMMARY The estrogen receptor gene was studied as a candidate gene for reproductive characteristics in sows of the commercial Yorkshire x Landrace cross. A total of 300 female pigs were generically typified for ESR and analyzed for determining the relationship amongst this locus and total born piglets (TBP), piglets born alive (PBA), weaned piglets (WP), litter weight at birth (LWB), litter weight at weaning (LWW) and sow reproductive value (SRV), expressing progeny characteristics of sows. Data were analyzed according to groups of high and low productivity respectively (105<SRV<95). In both groups, n = 150. There were significant differences (P<0.01) for allele B frequency which was highest in the group of high productivity, in line with frequency for the AB genotype; homozygote animals were not detected for allele B in both groups. The ESR was significantly (P<0.001) associated to TBP, WP, LWW and SRV where AB was better than AA genotype when means were compared, with advantages of +0.43, +0.81, +8.82 and +11.07 units in this same order, respectively. There were no significant differences between AA and AB genotypes within both groups of production. It could be concluded that animals from the AB genotype, therefore containing a copy of the favorable allele (B), are related to reproductive characteristics of sows, with independence to the considered production level of sows, improving substantially the number of born alive piglets, the number and weight of weaned piglets and the reproductive value of progeny of sows.

Rev. Cient.( …, 2006

Se estudiaron genes candidatos para tamaño de la camada en 300 hembras porcinas Yorkshire-Landrac... more Se estudiaron genes candidatos para tamaño de la camada en 300 hembras porcinas Yorkshire-Landrace; ESR, PRLR, RBP4 y FUT1. Las hembras fueron agrupadas en dos niveles de producción (NP): nivel alto (NA) y nivel bajo (NB). Utilizando Ji cuadrado se analizaron las frecuencias génicas y genotípicas. Empleando análisis de varianza con un modelo de efectos mixtos, para lechones nacidos totales (LNT), nacidos vivos (LNV), peso de la camada al nacimiento (PNAC) y destete (PAJ21), lechones destetados (LD) y valor de cría de la progenie de la cerda (BVSP), se compararon las medias con contrastes ortogonales. Las hembras con alta productividad se asociaron con una mayor frecuencia del alelo B del gen ESR (P < 0,05). Las diferencias fueron de 0,4 LNV, 0,3 LD, 2,9 Kg de PAJ21 y 8,6 puntos de BVSP a favor del genotipo AB del gen ESR (P < 0,05) sin considerar el NP, no se detectaron animales homocigotos BB. Las frecuencias génicas y genotípicas del gen PRLR no se relacionaron con el NP (P > 0,05), no hubo diferencias (P > 0,05) entre los genotipos AA, AB y BB sin considerar el NP ni dentro del mismo NP. En el gen RBP4 la frecuencia del alelo A y del genotipo AA fue más alta en hembras con NA (P < 0,05), no se detectaron animales con genotipo BB. Las hembras con genotipos AA tuvieron más 0,5 LNT; 0,5 LNV; 0,6 Kg de PNAC; 2,6 Kg de PAJ21 y 3,2 puntos de BVSP que el genotipo AB (P < 0,05), sin considerar el NP. La frecuencia del alelo G y del genotipo GG del gen FUT1 fue mayor en el nivel de productividad alto (P < 0,05). El genotipo GG fue superior al genotipo AG con más 0,6 LNV; 0,8 Kg de PNAC; 3 Kg de PAJ21 y 3,9 puntos de BVSP (P < 0,05) sin considerar el NP.

Genetics and Molecular Biology, 2008

The polymorphism of locus BoLA-DRB3.2 of the Major Histocompatibility Complex was evaluated in tw... more The polymorphism of locus BoLA-DRB3.2 of the Major Histocompatibility Complex was evaluated in two northern Mexican Creole cattle populations, Chihuahua (n = 47) and Tamaulipas (n = 51). The BoLA-DRB3.2 locus was typed by amplification and digestion with restriction endonuclease enzymes (PCR-RFLP). Fifty-two alleles were detected (28 previously reported and 24 new ones). In the Chihuahua population, 18 alleles and 5.5 effective alleles were found, while in the Tamaulipas population there were 34 and 10.8, respectively. The allele frequencies ranged from 0.011 to 0.383 in Chihuahua and from 0.010 to 0.206 in Tamaulipas. The frequencies of the new alleles in both cattle populations were low (0.010 to 0.053). The expected heterozygosity was 0.827 and 0.916, respectively, for the Chihuahua and Tamaulipas populations. Both populations presented a heterozygote deficit: [Chihuahua F IS = 0.1 (p = 0.019) and Tamaulipas F IS = 0.317 (p < 0.001)]. In conclusion, this study showed that the Mexican Creole cattle have many low-frequency alleles, several of which are exclusive to these populations. Genetic distances obtained show that the Mexican Creole cattle population is composed of independent populations, far apart from other South American Creole populations.

Journal of Cell Biology, 1989

The giardins are a group of 29-38-kD proteins in the ventral disk of the protozoan parasite Giard... more The giardins are a group of 29-38-kD proteins in the ventral disk of the protozoan parasite Giardia lamblia. The disk attaches the parasite to the host's intestinal epithelium and is composed of parallel, coiled microtubules that are adjacent to the ventral plasma membrane and from which processes called microribbons extend into the cytoplasm; the microribbons are connected by crossbridges. G. lamblia cytoskeletons, consisting of disks and attached flagella, were isolated and used to show that the 29-38-kD proteins separate into five bands by one-dimensional electrophoresis and into 23 species by two-dimensional analysis. Rabbit antibodies raised against a 33-kD protein band, purified by one-dimensional gel electrophoresis and shown to contain three proteins by two-dimensional electrophoresis, recognized 17 proteins by two-dimensional immunoblot analysis. By immunofluorescence these antibodies reacted with the ventral disk but not with the flagella in isolated cytoskeletons. Ele...

Revista Mexicana de Ciencias Pecuarias, 2009

In order to learn about the mitochondrial genetic origin of Mexican creole sheep populations, blo... more In order to learn about the mitochondrial genetic origin of Mexican creole sheep populations, blood samples from 106 of these animals were collected in the Mexican States of Chiapas, Puebla, Morelos, Hidalgo and Veracruz. Samples were collected from creole-Suffolk admixed (n=23), Pelibuey (n=15) and Black Belly (n=6) sheep. Cytochrome C oxidase subunit I gene (COXI) is located in the mitochondrion. This fragment has a polymorphic site for the HinfI enzyme in the 5562-5566 position that allows for differentiating between Asian and European genotypes of Ovis aries. A polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis was used. A 1053 base pair (bp) fragment of the COX 1 gene was amplified then digested with the HinfI restriction enzyme. All samples from creole sheep and creole crosses revealed the genotype B of the COX 1 gene. Nevertheless, only one Black Belly animal had genotype A. Therefore, local Mexican sheep (creole, Chiapas and Pelibuey breed...

Electronic Journal of Biotechnology, 2008

Electronic Journal of Biotechnology ISSN: 0717-3458 Vol.11 No.3, Issue of July 15, 2008 © 2008 by... more Electronic Journal of Biotechnology ISSN: 0717-3458 Vol.11 No.3, Issue of July 15, 2008 © 2008 by Pontificia Universidad Catolica de Valparaiso -- Chile Received November 19, 2007 / Accepted March 14, 2008

Revista Mexicana de Ciencias Pecuarias, 2014

El estudio genético en razas de ovejas que naturalmente presentan altas tasas de ovulación (TO) y... more El estudio genético en razas de ovejas que naturalmente presentan altas tasas de ovulación (TO) y de prolificidad, ha permitido detectar la participación de varios genes. Entre ellos están los relacionados a lasuperfamilia del factor de crecimiento transformante B (BMPRIB, GDF9 y BMP-15), así como de otros genes con efecto mayor, tales como el “distal-less homeobox 3” (FecL), el receptor de estrógenos (ESR), el receptor de prolactina (PRLR) y el de las inhibinas (INHA y INHB). Sin embargo, las ovejas homocigotas para la mayoría de las variantes alélicas en BMP-15 o GDF9 son estériles, por lo que es importante el entendimiento de las bases genéticas y moleculares de estos polimorfismos, para su uso en programas racionales de mejoramiento genético con énfasis en la prolificidad. El polimorfismo en el gen de la melatonina (MTNR1) se ha asociado con la no estacionalidad reproductiva en ovejas. La selección en varias razas de ovejas para estas características ha reducido significativamen...

Veterinaria México OA, 2016

The epidemiological and clinicopathological features of canine mammary tumors may provide valuabl... more The epidemiological and clinicopathological features of canine mammary tumors may provide valuable information to facilitate analysis of the behavior of the disease and represent a potential tool for the study of breast cancer in women. The aim of this study was to associate the histological features of canine mammary tumors with potential risk factors and survival. One hundred and seventy-eight mammary tumors were collected from 80 female dogs. The statistical analyses consisted of a series of univariate studies and frequencies of the different study variables, such as a bivariate analysis with the Chi squared test (χ 2), a relative risk and Kaplan Meier survival analysis, and a multiple correspondence analysis was used to correlate the tumor's biological behavior with the dogs' breed. Most patients were older than 8 years and had at least one malignant tumor, which was usually solitary and measured more than 6 cm; these patients had poor survival. The most frequent tumors were a complex adenoma, benign mixed tumor, carcinoma complex and mixed type carcinoma. The most commonly affected breeds were Poodle, Cocker Spaniel and Dachshund, and the breeds at the highest risk of tumor development were Cocker Spaniel, Labrador Retriever and German Shepherd. Overall, the data indicated that mammary tumors in dogs mainly affected older females with malignant tumors and that there were high mortality and short-term survival rates. However, the most commonly affected breeds were not necessarily the most susceptible. Our data do not support the hypothesis of an increased risk of canine mammary tumors in nulliparous female dogs.

Journal of Animal and Veterinary Advances

ABSTRACT

Con el objetivo de determinar la inocuidad bacteriológica de los quesos frescos y semimadurados q... more Con el objetivo de determinar la inocuidad bacteriológica de los quesos frescos y semimadurados que se venden en algunos ¿mercados sobre ruedas¿ en la ciudad de México, se realizó la detección simultánea de Salmonella spp y de Listeria monocytogenes, mediante la técnica de reacción en cadena de la polimerasa (PCR), así como con los métodos bacteriológicos convencionales, según la normatividad correspondiente para cada microorganismo; es decir, la NOM-114-SSA1-1994 mexicana, que constituye un método para la determinación de Salmonella en alimentos; de igual manera la NOM-143-SSA1-1995 mexicana, que representa un método de prueba microbiológica para alimentos y determinación de L. monocytogenes. Se analizaron 120 muestras seleccionadas al azar, provenientes de cuatro ¿mercados sobre ruedas¿ de una zona del sur de la ciudad de México. La metodología propuesta para la PCR múltiple se basó en la amplifi cación simultánea de los genes InvA e Iap procedentes de los genomas de Salmonella sp...

Journal of Dairy Research, 2012

The objective of this study was to evaluate the effects of the CSN1S1 locus polymorphism on 305-d... more The objective of this study was to evaluate the effects of the CSN1S1 locus polymorphism on 305-d records of milk, fat, protein, lactose and total solids yields, fat, protein, lactose and total solids contents in Mexican dairy goats. A total of 514 lactation records belonging to Alpine (n=60), Saanen (n=105) and Toggenburg (n=74) goats, born from 2003 to 2006 in three herds were used. Discrimination between alleles E, F, N, A* (CSN1S1 A, G, H, I, O1 and O2) and B* (CSN1S1 B1, B2, B3, B4, C and L) were made by amplification of fragments of the gene CSN1S1 and digestion with the restriction endonuclease XmnI. In order to estimate additive and dominance effects, data sets including (1) all genotypes, and (2) only homozygote genotypes, were analysed using linear mixed models. The allele A*, had significant additive effects for protein content (0·21±0·07%;P=0·002) and total solids content (0·66±0·23%;P=0·005) when compared with allele F. An unfavourable additive effect of allele A* on mi...

Journal of Animal Breeding and Genetics, 2008

The objective of this study was to evaluate the genetic structure of Mexican Criollo cattle popul... more The objective of this study was to evaluate the genetic structure of Mexican Criollo cattle populations using microsatellite genetic markers. DNA samples were collected from 168 animals from four Mexican Criollo cattle populations, geographically isolated in remote areas of Sierra Madre Occidental (West Highlands). Also were included samples from two breeds with Iberian origin: the fighting bull (n = 24) and the milking central American Criollo (n = 24) and one Asiatic breed: Guzerat (n = 32). Genetic analysis consisted of the estimation of the genetic diversity in each population by the allele number and the average expected heterozygosity found in nine microsatellite loci. Furthermore, genetic relationships among the populations were defined by their genetic distances. Our data shows that Mexican cattle populations have a relatively high level of genetic diversity based either on the mean number of alleles (10.2-13.6) and on the expected heterozygosity (0.71-0.85). The degree of observed homozygosity within the Criollo populations was remarkable and probably caused by inbreeding (reduced effective population size) possibly due to reproductive structure within populations. Our data shows that considerable genetic differentiation has been occurred among the Criollo cattle populations in different regions of Mexico.

Genetics and Molecular Biology, 2006

Bovine tuberculosis, caused by Mycobacterium bovis, is a disease of socioeconomic and public heal... more Bovine tuberculosis, caused by Mycobacterium bovis, is a disease of socioeconomic and public health importance and of significance to international trade regulation. Allelic variants of several genes have been implicated in the genetic susceptibility to tuberculosis in some human populations, but little is known in cattle. We surveyed 34 European, 18 Asian, 20 Creole and 23 hybrid bovines for polymorphisms of the bovine solute carrier family 11 a1(Slc11a1) gene, formerly known as natural resistance associated macrophage protein (Nramp1), gene by typing the cattle using two microsatellite loci closely linked to this gene. The microsatellites used were 311-22, located at the 3' untranslated region (3' UTR) of the Slc11a1 gene, and ARO28 situated about 0.6 cM upstream of the same gene Based on allele size in base pairs (bp) we determined five 311-223 locus variants (221, 223, 225, 227 and 229 bp) and 12 ARO28 loci. There was marked diversity and a very high level of heterozygosity in most of the cattle surveyed except the Europeans bovines and especially Holsteins in relation to the 3' UTR microsatellite locus.

Electronic Journal of Biotechnology, 2008

CyTA - Journal of Food, 2009

El objetivo de este trabajo fue desarrollar una técnica de reacción en cadena de la polimerasa (P... more El objetivo de este trabajo fue desarrollar una técnica de reacción en cadena de la polimerasa (PCR) múltiple para la detección simultánea de Salmonella spp. y Escherichia coli O157:H7 en canales de bovino. Para obtener los productos de PCR, se amplifican los genes slt-I, ...