Fitri Yelli | Lampung University (original) (raw)
Papers by Fitri Yelli
IOP Conference Series: Earth and Environmental Science
Lampung is one the biggest areas for planting cassava in Indonesia, approximately 119 thousand ha... more Lampung is one the biggest areas for planting cassava in Indonesia, approximately 119 thousand ha with a productivity of 26 tons/ha. Optimum harvest time for cassava high yield could be 10-12 months after planting (MAP). However, most farmers used to harvest cassava around 7 MAP leading to low yield. This condition needs to introduce early age of harvest cassava to achieve the alternative solution. The objective of this study were to evaluate the growth characteristics of early cassava harvest for high yield. Treatment were arranged by a single factor in a randomized complete block design (RCBD) with three replications. Factors were 12 cassava clones as CMR 51-61-1, CMR 51-48-17, CMR 51-48-16, UJ-3, UJ-5, Vamas, Adira, CMR 51-07-03, OMM 0806-57, CMR 51-06-16, MC 6.10.311, and Litbang UK2. In this study, the variables measured included plant height, root fresh weight, starch content, and starch yield. Result indicated that the Vamas clone had relatively low plant height. At 7 MAP, th...
Abdimas Galuh, Mar 31, 2023
Jurnal Penelitian Pertanian Terapan, 2017
Oil palm (Elaeis guineensis Jacq.) in Indonesia commonly cultivated on land that is classified as... more Oil palm (Elaeis guineensis Jacq.) in Indonesia commonly cultivated on land that is classified as marginal land such as soil type ultisol with a low fertility rate, so it is necessary to improve the quality of seeds with the use of arbuscular mycorrhiza fungi (AMF) and NPK fertilizers. This study aimed (1) to determine the most appropriate type of AMF for oil palm seedling, (2) to determine the dose of NPK fertilizeris best for the growth of oil palm seedlings, (3) to determine whether the response of oil palm seedling stobe determined by the type of NPK fertilizer, (4) to determine the best dose of NPK fertilizer for each type of AMF. Research compiled using factorial treatment design (6 x2) with 5 replications. The first factor is the type of mycorrhizal (M) which consists of 6 levels iem0 (without AMF), m1 (Entrophospora sp. Isolates MV 3 ), m2 (Entrophospora sp. Isolates MV12), m3 (Glomus sp. Isolates MV 4), m4 (Glomus sp. Isolates MV11), and m5 (Glomus sp. Isolates MV13). The s...
Notulae Botanicae Horti Agrobotanici Cluj-Napoca
Plant breeding through hybridization in cassava is facing a problem due to inconsistent flowering... more Plant breeding through hybridization in cassava is facing a problem due to inconsistent flowering, and also the donor genes controlling superior traits are limited. An alternative method of breeding is through genetic transformation, and regeneration via somatic embryogenesis is promising route to achieve this. As somatic embryogenesis in cassava is genotype-specific, in the present study a protocol has been developed for UJ-3 and BW-1 genotypes. Immature sterile leaves from 7-10 days axillary shoots in a pre-condition medium were used as an explant. Leaves were inoculated on Murashige and Skoog (MS) medium containing picloram (0.0, 7.5, 10.0, 12.5, and 15.0 mg/L) and 1-naphthalene acetic acid (NAA, 6 mg/L) for induction of somatic embryos (SEs). Genotype BW-1 showed best results as early callus formation time i.e. 8.04±0.32 days after induction (dai) compared to UJ-3 (8.67±2.13 dai). The callus fresh weight (0.64 g) was also higher in BW-1 than UJ-3 (0.38 g) after 4 weeks in callus...
Jurnal Agro
Ubi kayu varietas UJ-3, klon BW-1 dan Unila UK-1 banyak ditanam khususnya di Lampung. Untuk itu d... more Ubi kayu varietas UJ-3, klon BW-1 dan Unila UK-1 banyak ditanam khususnya di Lampung. Untuk itu diperlukan bibit dalam jumlah besar yang dapat dilakukan secara in vitro. Penelitian ini bertujuan untuk mengevaluasi pengaruh BA dan NAA terhadap induksi dan multiplikasi tunas ubi kayu secara in vitro. Penelitian dilakukan menggunakan rancangan acak lengkap (RAL) dengan dua faktor, yaitu : (1) Varietas/klon yang terdiri atas UJ-3 (K1), BW-1 (K2) dan Unila UK-1 (K3), dan (2) Media yang terdiri atas media Murashige and Skoog (MS) tanpa zat pengatur tumbuh (M1), MS + BA 0,1 mg L-1 (M2), MS + BA 0,1 mg L-1 + NAA 0,05 mg L-1 (M3), MS + BA 0,3 mg L-1 + NAA 0,05 mg L-1 (M4), MS + BA 0,5 mg L-1 + NAA 0,05 mg L-1 (M5). Setiap perlakuan diulang sebanyak empat kali. Hasil menunjukkan bahwa tunas dari varietas/klon ubi kayu dapat terinduksi pada semua media perlakuan. Rata-rata jumlah tunas tertinggi (1,25 tunas) dihasilkan pada media M4 untuk klon Unila UK-1, jumlah buku paling tinggi (8 buku) dih...
Jurnal agrotek tropika, Nov 12, 2022
Indigofera sp. was one of the legume plants that have the potential as animal feed ingredients, b... more Indigofera sp. was one of the legume plants that have the potential as animal feed ingredients, because it had a high protein content and relatively low crude fiber. Propagation of plants using seeds was difficult because the structure of the seeds is hard and has a period of dormancy. The vegetative propagation of Indigofera sp using green stem cuttings technique with commercial growth regulators that could been easily applied to farming communities was the aim of this study. This study used a completely randomized design with 5 replications. The treatments were arranged in a factorial with the first factor being the number of buds: cuttings with 1, 2 and 3 buds. The second factor was the treatment of growth regulators (PGR): without plant growth regulator (R-0), Rootone-F (R-F), and Root-Up (R-U). The results showed that the best number of buds was cuttings with 3 buds because it produced 100% cutting survival percentage with the highest values for the variables number of leaves, number of leaflets, shoot length, number of roots, fresh weight of shoots, and higher shoot dry weight. compared to cuttings 2 and 1 bud. The addition of Root-Up resulted in the highest number of roots compared to the addition of Rootone-F and without PGR. There was no interaction between the number of shoots and the treatment of growth regulators on the growth of cuttings of Indigofera sp.
Jurnal agrotek tropika, Nov 12, 2022
This study aims to determine the effect of types and concentrations of POC and to determine the e... more This study aims to determine the effect of types and concentrations of POC and to determine the effect of POC types on the growth and production of rampai tomatoes depending on the addition of POC concentrations. This study used a factorial randomized completely block design (2 x 4) with three replications as a group. The first factor is the type of POC and the second factor is the concentration of POC. This study was tested with the Bartlett test (homogeneity) and Tukey test (additivity), which was then carried out by analysis of variance (F test), followed by separation of the mean using BNT (the smallest real difference) with a real level of 5%. The results showed that banana peel POC type was better than goat manure POC on the observation variables of the number, fruit diameter, and dry weight of stover. The higher the POC concentration up to 120 ml / l of water will increase the growth and production of rampai tomatoes on the observation variables of plant height, number of leaves, productive branches, fruit weight, number of fruits, fruit diameter, fresh weight of stover, and dry weight of stover. The effect of the type of POC depending on the provision of POC concentration occurred in the observation variable of plant height.
Jurnal Teknik Pertanian, Mar 31, 2022
Chili is one of the important vegetables in the culture of Indonesian society, but its productivi... more Chili is one of the important vegetables in the culture of Indonesian society, but its productivity is still low. This research aimed to determine the response of biofertilizer application and alkali supplement fertilizer on the growth and yield of chili. This research was conducted in Integrated Field Laboratory, University of Lampung, and arranged in a randomized block design (RBD) consisting of two factors, namely biofertilizer (0, 4, and 8 ml/ plant) and alkali supplement fertilizer (0, 0,05, and 0,1 gram/ plant). Each experiment included 3 repetitions. Observation consists of plant height, number of flowers, number of fruit, fruit length, and fruit weight per plant. Data were analyzed using analysis of variance (ANOVA), followed by Least Significant Difference (LSD) at 5% level of significance. Results showed that biofertilizer and alkali supplement fertilizer had a significant effect on growth and production variables. The highest production was obtained in the biofertilizer treatment of 8 ml/plant combined by alkali supplement fertilizers dose of 0,1 gram/plant with weight of 436.70 grams/plant.
JURNAL AGROTROPIKA
This study aims to determine the effect of various planting media and local microorganisms on the... more This study aims to determine the effect of various planting media and local microorganisms on the growth of 'Kristal' guava seedlings and the interaction between the provision of various planting media and local microorganisms on the growth of 'Kristal' guava seedlings. This research was conducted at the Integrated Field Laboratory, Faculty of Agriculture, University of Lampung from August 2020 to March 2021. The treatments were arranged in a factorial (2x4) in a completely randomized design (CRD) which was repeated 3 times. The first factor was planting media (M) which consisted of (M1) soil and rice husks, (M2) soil and mushroom baglog. The second factor is types of local microorganisms (MOL) (P) which consists of 4 levels, namely (P1) local microorganisms EM4, (P2) local microorganisms of stale rice, (P3) local microorganisms of oil palm empty fruit bunches, and (P4) local microorganisms of fruit and vegetables. rotten vegetables. The results of research that have...
JURNAL AGROTROPIKA
This study aims to determine the effect of providing various planting media and local microorgani... more This study aims to determine the effect of providing various planting media and local microorganisms on the growth of 'Kristal' guava seeds and the interaction between the provision of various planting media and local microorganisms on the growth of 'Kristal' guava seedlings. This research was conducted at the Integrated Field Laboratory, Faculty of Agriculture, University of Lampung from September 2020 to March 2021. The treatments were arranged in a factorial (2x4) in a completely randomized design (CRD) which was repeated 3 times. The first factor was planting media (M) which consisted of (M1) soil and rice husks, (M2) soil and mushroom baglog. The second factor is the type of local microorganism (MOL) (P) which consists of 4 levels, namely (P1) local microorganism EM4, (P2) local microorganism of banana weevil, (P3) local microorganism of coconut fiber, and (P4) local microorganism of cow dung. The results of the study which were tested by analysis on all variabl...
13301甲第4844号博士（理学）金沢大学博士論文要旨Abstract 要約Outline 以下に掲載：Plant biotechnology 35(3) pp.187-192 2018. 日... more 13301甲第4844号博士（理学）金沢大学博士論文要旨Abstract 要約Outline 以下に掲載：Plant biotechnology 35(3) pp.187-192 2018. 日本植物細胞分子生物学会. 共著者：Fitri Yelli, Tomoaki Kato, Takumi Nishiuch
Jurnal Agrotek Tropika
Fungi Mikoriza Arbuskular (FMA) mampu meningkatkan pertumbuhan tanaman dengan cara membantu penye... more Fungi Mikoriza Arbuskular (FMA) mampu meningkatkan pertumbuhan tanaman dengan cara membantu penyerapan air dan unsur hara dari dalam tanah. Selain itu, FMA juga berperan dalam meningkatkan ketahanan tanaman terhadap serangan patogen. Laboratorium Produksi Tanaman Perkebunan Fakultas Pertanian Universitas Lampung memiliki 35 koleksi isolat mikoriza. Isolat-isolat koleksi tersebut belum teridentifikasi dengan baik sampai ke level spesies. Penelitian ini bertujuan untuk menguji empat volume buffer ekstraksi InstaGeneTM Matrix yang efektif dan ekonomis untuk isolasi DNA FMA yang akan digunakan untuk identifikasi secara molekuler. Isolasi DNA 4 isolat FMA yaitu Glomus sp., Gigaspora sp., Acaulospora sp., dan Entrophospora sp. dilakukan dengan menggunakan buffer ekstraksi Instagene Matrix (BIO-RAD) dengan volume 10, 15, 20, dan 25 μl. Untuk melihat keberhasilan isolasi DNA yang dilakukan yaitu dengan menggunakan DNA tersebut sebagai template atau cetakan dalam proses PCR. Hasil penelitian...
Nepenthes plant is a unique plant due to its interesting pitcher at the leaf tip. The objective o... more Nepenthes plant is a unique plant due to its interesting pitcher at the leaf tip. The objective of the research was to determine the suitable MS (Murashige and Skoog) medium concentration for growth of two species of nepenthes and induction pitcher formation. A factorial experiment arranged in completely randomized design was carried out with the first factor was two species of Nepenthes; N. ampullaria and N. mirabilis. The second factor was several concentrations of MS medium, i.e. 0.500 MS, 0.250 MS, 0.125 MS, 0.0625 MS and 0.000 MS. The results showed that the number of leaves, the number of pitchers were affected by the type of Nepenthes and MS medium while plant height only affected by type of Nephenthes. N. mirabilis had significance higher number of leaf of 13.47 compared to N. mirabilis of 11.10. However, number of pitchers formed was lower in N. mirabilis compared to N.ampullaria. Data obtained also showed that the best MS medium for pitcher formation was 0.0625 MS medi...
Plant Biotechnology, 2018
The ethylene (ET) signaling pathway is involved in plant immunity and contributes to the disease ... more The ethylene (ET) signaling pathway is involved in plant immunity and contributes to the disease tolerance of plants to necrotrophic phytopathogens. Ethylene response factors (ERFs) are known to play important roles in the transcriptional regulation of defense genes by ET. In the present study, we analyzed the function of AtERF71 belonged to group VII ERF family in disease resistance against a hemibiotrophic fungal phytopathogen, Fusarium graminearum. When conidia solutions were dropped onto intact leaves of Arabidopsis plants, both ein2-1 and ein3-1 mutants showed enhanced disease resistance against F. graminearum compared with the wild type. This finding suggested that the ET signaling pathway was involved in the resistance to Fusarium entry into the leaf epidermis in Arabidopsis plants. We discovered that the AtERF71 expression was significantly induced by inoculation with F. graminearum. This induction of AtERF71 was suppressed in the ein3-1 mutant. Enhanced disease resistance was observed in the leaves of the aterf71 mutant when compared with wild type. In addition, the expression levels of the JA/ET-responsive PDF1.2 gene were significantly downregulated in the aterf71 mutant after inoculation with F. graminearum. Taken together, these results indicate the possible involvement of AtERF71 in disease tolerance to F. graminearum in Arabidopsis plants.
... Author: Yelli, Fitri. URI: http://repository.ipb.ac.id/handle/123456789/7620\. Date: 2004. Fil... more ... Author: Yelli, Fitri. URI: http://repository.ipb.ac.id/handle/123456789/7620. Date: 2004. Files in this item. Files, Size, Format, View. 2004fye_abstract.pdf, 193.9Kb, PDF, View/Open. 2004fye_abstract.ps, 374.2Kb, Postscript, View/Open. 2004fye.pdf, 3.032Mb, PDF, View/Open. ...
The Effect of Manure and Biological Fertilizer on Growth and Production of Tomato (Lycopersicum e... more The Effect of Manure and Biological Fertilizer on Growth and Production of Tomato (Lycopersicum esculentum Mill). The productivity of tomato in Lampung Province has continued to decline in recent years. In 2017, the productivity of tomatoes was recorded at 13.46 tonnes/ha, while in 2019 it only reached 10.52 tonnes/ha. In fact, this value is still much lower than the national average of 18.63 tonnes/ha. To overcome this, increasing productivity is absolutely necessary, one of which is the addition of manure and biological fertilizer. This research was conducted at the Field Laboratory, Faculty of Agriculture, University of Lampung in January May 2020. This study used a 4 x 3 factorial randomized block design (RBD) with three replications. The first factor is fertilization using several types of manure (chicken, goat and cow) as much as 1/3 of the planting medium, while the second factor is the application of biological fertilizers with doses of 0 l/ha, 4 l/ha, and 8 l/ha. The result...
JURNAL AGROTROPIKA
Sweet potato is raw locally food used as an alternative staple food that important for national f... more Sweet potato is raw locally food used as an alternative staple food that important for national food security. The objective of the reseach was to achive the suitable medium for preservation of sweet potato plants by slowly growth in in vitro medium. The factorial experiment arranged in completely randomized design with the first factor was two concentrations of MS medium: MS 1 and MS 2. The second factor was several concentrations of coconut water, i.e. 0 and 15 mg/l and the third factor was several concentrations of Aspirin (0, 5, 10, 15, 20 mg/l) . The results showed that the number of green leaves and the number of internodes were affected by concentrations of medium in six weeks after planting. The MS1 medium with 0 mg/l Aspirin and 15 mg/ coconut water could produced the higher number of green leaves (8,0) and 8,5 internodes number. The MS2 + 15 mg/l coconut water and 15 mg/l Aspirin was the best medium for sweet potato preservation. Data obtained also showed that...
JURNAL AGROTROPIKA
Coffee husk is the main waste of coffee bean production using a dry processing system. Coffee hus... more Coffee husk is the main waste of coffee bean production using a dry processing system. Coffee husk is returned to the coffee plantation as mulch, or used as a mixture for nursery media and for making bio-charcoal. This study aims to determine the effect of the formulation of biochar, coffee husk, and soil on the growth of Arabica coffee seedlings. The experiment used a randomized block design (RBD) consisting of 6 treatments with 4 replications. The treatments were the composition of the ratio of biochar: coffee husk: soil (v / v / v), namely: 0: 1: 2 (control, without biochar), 1: 1: 2, 1: 0: 2 (without coffee skin), 2: 1: 2, 1: 2: 2, and 1: 1: 1. The coffee husk biochar is made by a slow burning system in a stack. The results showed that: (1) The treatment of biochar composition, coffee husk and soil had an effect on the number of leaves, length x leaf width, crown dry weight, fresh weight quality index, and dry weight quality index; (2) biochar mixing treatment did not increase s...
Penelitian ini bertujuan untuk mengetahui pengaruh perlakuan eksplan biji yang dikecambahkan atau... more Penelitian ini bertujuan untuk mengetahui pengaruh perlakuan eksplan biji yang dikecambahkan atau diimbibisikan (pra-kultur) terhadap efisiensi regenerasi in vitro empat varietas kedelai (Glycine max (L). Merr.) melalui organogenesis. Penelitian dilakukan di Laboratorium Kultur Jaringan, Fakultas Pertanian, Universitas Lampung, dari bulan Maret 2013 sampai dengan Mei 2013. Percobaan disusun dalam rancangan acak kelompok yang terdiri atas 5 ulangan. Perlakuan disusun secara faktorial (4x2) dengan faktor pertama adalah varietas kedelai sebagai eksplan (Grobogan, Argomulyo, Tanggamus, dan Ijen) dan faktor kedua adalah perlakuan pra-kultur (imbibisi atau pengecambahan). Setiap satuan percobaan terdiri atas empat eksplan yang dikulturkan dalam satu botol. Hasil penelitian menunjukkan bahwa perlakuan pra-kultur berpengaruh terhadap persentase eksplan yang menghasilkan tunas adventif (PEMTA) dan rata-rata jumlah tunas adventif per eksplan (RJTA). Sedangkan perlakuan varietas dan interaksi hanya berpengaruh pada PEMTA tetapi tidak berpengaruh pada RJTA. Pada perlakuan imbibisi, PEMTA varietas Ijen lebih tinggi daripada Grobogan, Argomulyo, dan Tanggamus. Pada perlakuan pengecambahan, PEMTA varietas Ijen lebih tinggi daripada Argomulyo namun tidak berbeda dengan Tanggamus dan Grobogan. Jika menggunakan varietas Ijen dan Argomulyo, PEMTA perlakuan imbibisi lebih tinggi daripada pengecambahan. RJTA perlakuan pra- kultur imbibisi 20 jam (19,5 tunas per eksplan) lebih tinggi daripada perlakuan kecambah 6 hari (9,63 tunas per eksplan). Selain itu, media pengakaran ½ MS tanpa NAA lebih baik dalam membentuk akar fungsional daripada ½ MS yang mengandung NAA 0,5 mg/l. Kata kunci: buku kotiledon, imbibisi, Kedelai, organogenesis, pengakaran, pengecambahan.
![Research paper thumbnail of REGENERASI IN VITRO EMPAT VARIETAS KEDELAI (Glycine max [L.] Merr.) MELALUI ORGANOGENESIS MENGGUNAKAN EKSPLAN BIJI YANG DIIMBIBISI DAN DIKECAMBAHKAN](https://attachments.academia-assets.com/80653548/thumbnails/1.jpg)
](Penelitian dilakukan untuk mengetahui pengaruh metode pra-kultur yang efektif dalam meningkatkan ... more Penelitian dilakukan untuk mengetahui pengaruh metode pra-kultur yang efektif dalam meningkatkan efisiensi regenerasi in vitro terhadap eksplan buku kotiledon pada empat varietas kedelai. Penelitian dilaksanakan di Laboratorium Ilmu Tanaman Fakultas Pertanian Universitas Lampung, dimulai dari Maret sampai dengan Mei 2013. Rancangan yang digunakan dalam penelitian ini adalah rancangan acak kelompok. Perlakuan yang diterapkan merupakan kombinasi dari dua faktor yaitu varietas (Detam 1, Detam 2, Burangrang, Panderman) dan metode pra-kultur (kecambah 6 hari dan imbibisi 20 jam) yang merupakan perlakuan terhadap empat varietas benih kedelai tersebut sebelum ditanam pada media inisiasi tunas. Perlakuan disusun secara faktorial (4x2) dengan 5 ulangan. Setiap satuan percobaan terdiri dari empat eksplan buku kotiledon kedelai. Hasil penelitian menunjukkan bahwa rata-rata jumlah tunas adventif per eksplan (RJTAPE) tidak dipengaruhi oleh metode prakultur, varietas dan interaksi antara keduanya. Sedangkan, persentase eksplan yang membentuk tunas adventif (PEMTA) tidak dipengaruhi oleh metode pra-kultur namun dipengaruhi oleh varietas dan interaksi antara kedua faktor tersebut. PEMTA tertinggi didapatkan jika menggunakan varietas Detam 1 melalui perlakuan perkecambahan (70%) dan varietas Panderman melalui perlakuan imbibisi (50%). Media pengakaran ½ MS dan ½ MS + NAA 0,5 mg l-1 memiliki efektifitas yang sama terhadap persentase tunas adventif yang membentuk akar fungsional (PTMAF) pada minggu kedua setelah pengakaran. Dengan demikian, teknik regenerasi secara in vitro melalui organogenesis pada varietas Detam 1 dengan perlakuan perkecambahan dan varietas Panderman dengan perlakuan imbibisi lebih efisien digunakan untuk transformasi genetik kedelai.
IOP Conference Series: Earth and Environmental Science
Lampung is one the biggest areas for planting cassava in Indonesia, approximately 119 thousand ha... more Lampung is one the biggest areas for planting cassava in Indonesia, approximately 119 thousand ha with a productivity of 26 tons/ha. Optimum harvest time for cassava high yield could be 10-12 months after planting (MAP). However, most farmers used to harvest cassava around 7 MAP leading to low yield. This condition needs to introduce early age of harvest cassava to achieve the alternative solution. The objective of this study were to evaluate the growth characteristics of early cassava harvest for high yield. Treatment were arranged by a single factor in a randomized complete block design (RCBD) with three replications. Factors were 12 cassava clones as CMR 51-61-1, CMR 51-48-17, CMR 51-48-16, UJ-3, UJ-5, Vamas, Adira, CMR 51-07-03, OMM 0806-57, CMR 51-06-16, MC 6.10.311, and Litbang UK2. In this study, the variables measured included plant height, root fresh weight, starch content, and starch yield. Result indicated that the Vamas clone had relatively low plant height. At 7 MAP, th...
Abdimas Galuh, Mar 31, 2023
Jurnal Penelitian Pertanian Terapan, 2017
Oil palm (Elaeis guineensis Jacq.) in Indonesia commonly cultivated on land that is classified as... more Oil palm (Elaeis guineensis Jacq.) in Indonesia commonly cultivated on land that is classified as marginal land such as soil type ultisol with a low fertility rate, so it is necessary to improve the quality of seeds with the use of arbuscular mycorrhiza fungi (AMF) and NPK fertilizers. This study aimed (1) to determine the most appropriate type of AMF for oil palm seedling, (2) to determine the dose of NPK fertilizeris best for the growth of oil palm seedlings, (3) to determine whether the response of oil palm seedling stobe determined by the type of NPK fertilizer, (4) to determine the best dose of NPK fertilizer for each type of AMF. Research compiled using factorial treatment design (6 x2) with 5 replications. The first factor is the type of mycorrhizal (M) which consists of 6 levels iem0 (without AMF), m1 (Entrophospora sp. Isolates MV 3 ), m2 (Entrophospora sp. Isolates MV12), m3 (Glomus sp. Isolates MV 4), m4 (Glomus sp. Isolates MV11), and m5 (Glomus sp. Isolates MV13). The s...
Notulae Botanicae Horti Agrobotanici Cluj-Napoca
Plant breeding through hybridization in cassava is facing a problem due to inconsistent flowering... more Plant breeding through hybridization in cassava is facing a problem due to inconsistent flowering, and also the donor genes controlling superior traits are limited. An alternative method of breeding is through genetic transformation, and regeneration via somatic embryogenesis is promising route to achieve this. As somatic embryogenesis in cassava is genotype-specific, in the present study a protocol has been developed for UJ-3 and BW-1 genotypes. Immature sterile leaves from 7-10 days axillary shoots in a pre-condition medium were used as an explant. Leaves were inoculated on Murashige and Skoog (MS) medium containing picloram (0.0, 7.5, 10.0, 12.5, and 15.0 mg/L) and 1-naphthalene acetic acid (NAA, 6 mg/L) for induction of somatic embryos (SEs). Genotype BW-1 showed best results as early callus formation time i.e. 8.04±0.32 days after induction (dai) compared to UJ-3 (8.67±2.13 dai). The callus fresh weight (0.64 g) was also higher in BW-1 than UJ-3 (0.38 g) after 4 weeks in callus...
Jurnal Agro
Ubi kayu varietas UJ-3, klon BW-1 dan Unila UK-1 banyak ditanam khususnya di Lampung. Untuk itu d... more Ubi kayu varietas UJ-3, klon BW-1 dan Unila UK-1 banyak ditanam khususnya di Lampung. Untuk itu diperlukan bibit dalam jumlah besar yang dapat dilakukan secara in vitro. Penelitian ini bertujuan untuk mengevaluasi pengaruh BA dan NAA terhadap induksi dan multiplikasi tunas ubi kayu secara in vitro. Penelitian dilakukan menggunakan rancangan acak lengkap (RAL) dengan dua faktor, yaitu : (1) Varietas/klon yang terdiri atas UJ-3 (K1), BW-1 (K2) dan Unila UK-1 (K3), dan (2) Media yang terdiri atas media Murashige and Skoog (MS) tanpa zat pengatur tumbuh (M1), MS + BA 0,1 mg L-1 (M2), MS + BA 0,1 mg L-1 + NAA 0,05 mg L-1 (M3), MS + BA 0,3 mg L-1 + NAA 0,05 mg L-1 (M4), MS + BA 0,5 mg L-1 + NAA 0,05 mg L-1 (M5). Setiap perlakuan diulang sebanyak empat kali. Hasil menunjukkan bahwa tunas dari varietas/klon ubi kayu dapat terinduksi pada semua media perlakuan. Rata-rata jumlah tunas tertinggi (1,25 tunas) dihasilkan pada media M4 untuk klon Unila UK-1, jumlah buku paling tinggi (8 buku) dih...
Jurnal agrotek tropika, Nov 12, 2022
Indigofera sp. was one of the legume plants that have the potential as animal feed ingredients, b... more Indigofera sp. was one of the legume plants that have the potential as animal feed ingredients, because it had a high protein content and relatively low crude fiber. Propagation of plants using seeds was difficult because the structure of the seeds is hard and has a period of dormancy. The vegetative propagation of Indigofera sp using green stem cuttings technique with commercial growth regulators that could been easily applied to farming communities was the aim of this study. This study used a completely randomized design with 5 replications. The treatments were arranged in a factorial with the first factor being the number of buds: cuttings with 1, 2 and 3 buds. The second factor was the treatment of growth regulators (PGR): without plant growth regulator (R-0), Rootone-F (R-F), and Root-Up (R-U). The results showed that the best number of buds was cuttings with 3 buds because it produced 100% cutting survival percentage with the highest values for the variables number of leaves, number of leaflets, shoot length, number of roots, fresh weight of shoots, and higher shoot dry weight. compared to cuttings 2 and 1 bud. The addition of Root-Up resulted in the highest number of roots compared to the addition of Rootone-F and without PGR. There was no interaction between the number of shoots and the treatment of growth regulators on the growth of cuttings of Indigofera sp.
Jurnal agrotek tropika, Nov 12, 2022
This study aims to determine the effect of types and concentrations of POC and to determine the e... more This study aims to determine the effect of types and concentrations of POC and to determine the effect of POC types on the growth and production of rampai tomatoes depending on the addition of POC concentrations. This study used a factorial randomized completely block design (2 x 4) with three replications as a group. The first factor is the type of POC and the second factor is the concentration of POC. This study was tested with the Bartlett test (homogeneity) and Tukey test (additivity), which was then carried out by analysis of variance (F test), followed by separation of the mean using BNT (the smallest real difference) with a real level of 5%. The results showed that banana peel POC type was better than goat manure POC on the observation variables of the number, fruit diameter, and dry weight of stover. The higher the POC concentration up to 120 ml / l of water will increase the growth and production of rampai tomatoes on the observation variables of plant height, number of leaves, productive branches, fruit weight, number of fruits, fruit diameter, fresh weight of stover, and dry weight of stover. The effect of the type of POC depending on the provision of POC concentration occurred in the observation variable of plant height.
Jurnal Teknik Pertanian, Mar 31, 2022
Chili is one of the important vegetables in the culture of Indonesian society, but its productivi... more Chili is one of the important vegetables in the culture of Indonesian society, but its productivity is still low. This research aimed to determine the response of biofertilizer application and alkali supplement fertilizer on the growth and yield of chili. This research was conducted in Integrated Field Laboratory, University of Lampung, and arranged in a randomized block design (RBD) consisting of two factors, namely biofertilizer (0, 4, and 8 ml/ plant) and alkali supplement fertilizer (0, 0,05, and 0,1 gram/ plant). Each experiment included 3 repetitions. Observation consists of plant height, number of flowers, number of fruit, fruit length, and fruit weight per plant. Data were analyzed using analysis of variance (ANOVA), followed by Least Significant Difference (LSD) at 5% level of significance. Results showed that biofertilizer and alkali supplement fertilizer had a significant effect on growth and production variables. The highest production was obtained in the biofertilizer treatment of 8 ml/plant combined by alkali supplement fertilizers dose of 0,1 gram/plant with weight of 436.70 grams/plant.
JURNAL AGROTROPIKA
This study aims to determine the effect of various planting media and local microorganisms on the... more This study aims to determine the effect of various planting media and local microorganisms on the growth of 'Kristal' guava seedlings and the interaction between the provision of various planting media and local microorganisms on the growth of 'Kristal' guava seedlings. This research was conducted at the Integrated Field Laboratory, Faculty of Agriculture, University of Lampung from August 2020 to March 2021. The treatments were arranged in a factorial (2x4) in a completely randomized design (CRD) which was repeated 3 times. The first factor was planting media (M) which consisted of (M1) soil and rice husks, (M2) soil and mushroom baglog. The second factor is types of local microorganisms (MOL) (P) which consists of 4 levels, namely (P1) local microorganisms EM4, (P2) local microorganisms of stale rice, (P3) local microorganisms of oil palm empty fruit bunches, and (P4) local microorganisms of fruit and vegetables. rotten vegetables. The results of research that have...
JURNAL AGROTROPIKA
This study aims to determine the effect of providing various planting media and local microorgani... more This study aims to determine the effect of providing various planting media and local microorganisms on the growth of 'Kristal' guava seeds and the interaction between the provision of various planting media and local microorganisms on the growth of 'Kristal' guava seedlings. This research was conducted at the Integrated Field Laboratory, Faculty of Agriculture, University of Lampung from September 2020 to March 2021. The treatments were arranged in a factorial (2x4) in a completely randomized design (CRD) which was repeated 3 times. The first factor was planting media (M) which consisted of (M1) soil and rice husks, (M2) soil and mushroom baglog. The second factor is the type of local microorganism (MOL) (P) which consists of 4 levels, namely (P1) local microorganism EM4, (P2) local microorganism of banana weevil, (P3) local microorganism of coconut fiber, and (P4) local microorganism of cow dung. The results of the study which were tested by analysis on all variabl...
13301甲第4844号博士（理学）金沢大学博士論文要旨Abstract 要約Outline 以下に掲載：Plant biotechnology 35(3) pp.187-192 2018. 日... more 13301甲第4844号博士（理学）金沢大学博士論文要旨Abstract 要約Outline 以下に掲載：Plant biotechnology 35(3) pp.187-192 2018. 日本植物細胞分子生物学会. 共著者：Fitri Yelli, Tomoaki Kato, Takumi Nishiuch
Jurnal Agrotek Tropika
Fungi Mikoriza Arbuskular (FMA) mampu meningkatkan pertumbuhan tanaman dengan cara membantu penye... more Fungi Mikoriza Arbuskular (FMA) mampu meningkatkan pertumbuhan tanaman dengan cara membantu penyerapan air dan unsur hara dari dalam tanah. Selain itu, FMA juga berperan dalam meningkatkan ketahanan tanaman terhadap serangan patogen. Laboratorium Produksi Tanaman Perkebunan Fakultas Pertanian Universitas Lampung memiliki 35 koleksi isolat mikoriza. Isolat-isolat koleksi tersebut belum teridentifikasi dengan baik sampai ke level spesies. Penelitian ini bertujuan untuk menguji empat volume buffer ekstraksi InstaGeneTM Matrix yang efektif dan ekonomis untuk isolasi DNA FMA yang akan digunakan untuk identifikasi secara molekuler. Isolasi DNA 4 isolat FMA yaitu Glomus sp., Gigaspora sp., Acaulospora sp., dan Entrophospora sp. dilakukan dengan menggunakan buffer ekstraksi Instagene Matrix (BIO-RAD) dengan volume 10, 15, 20, dan 25 μl. Untuk melihat keberhasilan isolasi DNA yang dilakukan yaitu dengan menggunakan DNA tersebut sebagai template atau cetakan dalam proses PCR. Hasil penelitian...
Nepenthes plant is a unique plant due to its interesting pitcher at the leaf tip. The objective o... more Nepenthes plant is a unique plant due to its interesting pitcher at the leaf tip. The objective of the research was to determine the suitable MS (Murashige and Skoog) medium concentration for growth of two species of nepenthes and induction pitcher formation. A factorial experiment arranged in completely randomized design was carried out with the first factor was two species of Nepenthes; N. ampullaria and N. mirabilis. The second factor was several concentrations of MS medium, i.e. 0.500 MS, 0.250 MS, 0.125 MS, 0.0625 MS and 0.000 MS. The results showed that the number of leaves, the number of pitchers were affected by the type of Nepenthes and MS medium while plant height only affected by type of Nephenthes. N. mirabilis had significance higher number of leaf of 13.47 compared to N. mirabilis of 11.10. However, number of pitchers formed was lower in N. mirabilis compared to N.ampullaria. Data obtained also showed that the best MS medium for pitcher formation was 0.0625 MS medi...
Plant Biotechnology, 2018
The ethylene (ET) signaling pathway is involved in plant immunity and contributes to the disease ... more The ethylene (ET) signaling pathway is involved in plant immunity and contributes to the disease tolerance of plants to necrotrophic phytopathogens. Ethylene response factors (ERFs) are known to play important roles in the transcriptional regulation of defense genes by ET. In the present study, we analyzed the function of AtERF71 belonged to group VII ERF family in disease resistance against a hemibiotrophic fungal phytopathogen, Fusarium graminearum. When conidia solutions were dropped onto intact leaves of Arabidopsis plants, both ein2-1 and ein3-1 mutants showed enhanced disease resistance against F. graminearum compared with the wild type. This finding suggested that the ET signaling pathway was involved in the resistance to Fusarium entry into the leaf epidermis in Arabidopsis plants. We discovered that the AtERF71 expression was significantly induced by inoculation with F. graminearum. This induction of AtERF71 was suppressed in the ein3-1 mutant. Enhanced disease resistance was observed in the leaves of the aterf71 mutant when compared with wild type. In addition, the expression levels of the JA/ET-responsive PDF1.2 gene were significantly downregulated in the aterf71 mutant after inoculation with F. graminearum. Taken together, these results indicate the possible involvement of AtERF71 in disease tolerance to F. graminearum in Arabidopsis plants.
... Author: Yelli, Fitri. URI: http://repository.ipb.ac.id/handle/123456789/7620\. Date: 2004. Fil... more ... Author: Yelli, Fitri. URI: http://repository.ipb.ac.id/handle/123456789/7620. Date: 2004. Files in this item. Files, Size, Format, View. 2004fye_abstract.pdf, 193.9Kb, PDF, View/Open. 2004fye_abstract.ps, 374.2Kb, Postscript, View/Open. 2004fye.pdf, 3.032Mb, PDF, View/Open. ...
The Effect of Manure and Biological Fertilizer on Growth and Production of Tomato (Lycopersicum e... more The Effect of Manure and Biological Fertilizer on Growth and Production of Tomato (Lycopersicum esculentum Mill). The productivity of tomato in Lampung Province has continued to decline in recent years. In 2017, the productivity of tomatoes was recorded at 13.46 tonnes/ha, while in 2019 it only reached 10.52 tonnes/ha. In fact, this value is still much lower than the national average of 18.63 tonnes/ha. To overcome this, increasing productivity is absolutely necessary, one of which is the addition of manure and biological fertilizer. This research was conducted at the Field Laboratory, Faculty of Agriculture, University of Lampung in January May 2020. This study used a 4 x 3 factorial randomized block design (RBD) with three replications. The first factor is fertilization using several types of manure (chicken, goat and cow) as much as 1/3 of the planting medium, while the second factor is the application of biological fertilizers with doses of 0 l/ha, 4 l/ha, and 8 l/ha. The result...
JURNAL AGROTROPIKA
Sweet potato is raw locally food used as an alternative staple food that important for national f... more Sweet potato is raw locally food used as an alternative staple food that important for national food security. The objective of the reseach was to achive the suitable medium for preservation of sweet potato plants by slowly growth in in vitro medium. The factorial experiment arranged in completely randomized design with the first factor was two concentrations of MS medium: MS 1 and MS 2. The second factor was several concentrations of coconut water, i.e. 0 and 15 mg/l and the third factor was several concentrations of Aspirin (0, 5, 10, 15, 20 mg/l) . The results showed that the number of green leaves and the number of internodes were affected by concentrations of medium in six weeks after planting. The MS1 medium with 0 mg/l Aspirin and 15 mg/ coconut water could produced the higher number of green leaves (8,0) and 8,5 internodes number. The MS2 + 15 mg/l coconut water and 15 mg/l Aspirin was the best medium for sweet potato preservation. Data obtained also showed that...
JURNAL AGROTROPIKA
Coffee husk is the main waste of coffee bean production using a dry processing system. Coffee hus... more Coffee husk is the main waste of coffee bean production using a dry processing system. Coffee husk is returned to the coffee plantation as mulch, or used as a mixture for nursery media and for making bio-charcoal. This study aims to determine the effect of the formulation of biochar, coffee husk, and soil on the growth of Arabica coffee seedlings. The experiment used a randomized block design (RBD) consisting of 6 treatments with 4 replications. The treatments were the composition of the ratio of biochar: coffee husk: soil (v / v / v), namely: 0: 1: 2 (control, without biochar), 1: 1: 2, 1: 0: 2 (without coffee skin), 2: 1: 2, 1: 2: 2, and 1: 1: 1. The coffee husk biochar is made by a slow burning system in a stack. The results showed that: (1) The treatment of biochar composition, coffee husk and soil had an effect on the number of leaves, length x leaf width, crown dry weight, fresh weight quality index, and dry weight quality index; (2) biochar mixing treatment did not increase s...
Penelitian ini bertujuan untuk mengetahui pengaruh perlakuan eksplan biji yang dikecambahkan atau... more Penelitian ini bertujuan untuk mengetahui pengaruh perlakuan eksplan biji yang dikecambahkan atau diimbibisikan (pra-kultur) terhadap efisiensi regenerasi in vitro empat varietas kedelai (Glycine max (L). Merr.) melalui organogenesis. Penelitian dilakukan di Laboratorium Kultur Jaringan, Fakultas Pertanian, Universitas Lampung, dari bulan Maret 2013 sampai dengan Mei 2013. Percobaan disusun dalam rancangan acak kelompok yang terdiri atas 5 ulangan. Perlakuan disusun secara faktorial (4x2) dengan faktor pertama adalah varietas kedelai sebagai eksplan (Grobogan, Argomulyo, Tanggamus, dan Ijen) dan faktor kedua adalah perlakuan pra-kultur (imbibisi atau pengecambahan). Setiap satuan percobaan terdiri atas empat eksplan yang dikulturkan dalam satu botol. Hasil penelitian menunjukkan bahwa perlakuan pra-kultur berpengaruh terhadap persentase eksplan yang menghasilkan tunas adventif (PEMTA) dan rata-rata jumlah tunas adventif per eksplan (RJTA). Sedangkan perlakuan varietas dan interaksi hanya berpengaruh pada PEMTA tetapi tidak berpengaruh pada RJTA. Pada perlakuan imbibisi, PEMTA varietas Ijen lebih tinggi daripada Grobogan, Argomulyo, dan Tanggamus. Pada perlakuan pengecambahan, PEMTA varietas Ijen lebih tinggi daripada Argomulyo namun tidak berbeda dengan Tanggamus dan Grobogan. Jika menggunakan varietas Ijen dan Argomulyo, PEMTA perlakuan imbibisi lebih tinggi daripada pengecambahan. RJTA perlakuan pra- kultur imbibisi 20 jam (19,5 tunas per eksplan) lebih tinggi daripada perlakuan kecambah 6 hari (9,63 tunas per eksplan). Selain itu, media pengakaran ½ MS tanpa NAA lebih baik dalam membentuk akar fungsional daripada ½ MS yang mengandung NAA 0,5 mg/l. Kata kunci: buku kotiledon, imbibisi, Kedelai, organogenesis, pengakaran, pengecambahan.
Penelitian dilakukan untuk mengetahui pengaruh metode pra-kultur yang efektif dalam meningkatkan ... more Penelitian dilakukan untuk mengetahui pengaruh metode pra-kultur yang efektif dalam meningkatkan efisiensi regenerasi in vitro terhadap eksplan buku kotiledon pada empat varietas kedelai. Penelitian dilaksanakan di Laboratorium Ilmu Tanaman Fakultas Pertanian Universitas Lampung, dimulai dari Maret sampai dengan Mei 2013. Rancangan yang digunakan dalam penelitian ini adalah rancangan acak kelompok. Perlakuan yang diterapkan merupakan kombinasi dari dua faktor yaitu varietas (Detam 1, Detam 2, Burangrang, Panderman) dan metode pra-kultur (kecambah 6 hari dan imbibisi 20 jam) yang merupakan perlakuan terhadap empat varietas benih kedelai tersebut sebelum ditanam pada media inisiasi tunas. Perlakuan disusun secara faktorial (4x2) dengan 5 ulangan. Setiap satuan percobaan terdiri dari empat eksplan buku kotiledon kedelai. Hasil penelitian menunjukkan bahwa rata-rata jumlah tunas adventif per eksplan (RJTAPE) tidak dipengaruhi oleh metode prakultur, varietas dan interaksi antara keduanya. Sedangkan, persentase eksplan yang membentuk tunas adventif (PEMTA) tidak dipengaruhi oleh metode pra-kultur namun dipengaruhi oleh varietas dan interaksi antara kedua faktor tersebut. PEMTA tertinggi didapatkan jika menggunakan varietas Detam 1 melalui perlakuan perkecambahan (70%) dan varietas Panderman melalui perlakuan imbibisi (50%). Media pengakaran ½ MS dan ½ MS + NAA 0,5 mg l-1 memiliki efektifitas yang sama terhadap persentase tunas adventif yang membentuk akar fungsional (PTMAF) pada minggu kedua setelah pengakaran. Dengan demikian, teknik regenerasi secara in vitro melalui organogenesis pada varietas Detam 1 dengan perlakuan perkecambahan dan varietas Panderman dengan perlakuan imbibisi lebih efisien digunakan untuk transformasi genetik kedelai.