Eko Prasetya | State University of Medan (original) (raw)

Papers by Eko Prasetya

Biodiversitas Journal of Biological Diversity

Sipahutar pineapple from North Sumatra, Indonesia, is considered difficult to breed, resulting in... more Sipahutar pineapple from North Sumatra, Indonesia, is considered difficult to breed, resulting in a lack of seed availability. However, tissue culture allows the emergence of somaclonal variations, especially in plantlets undergoing several subculture stages. This study aimed to identify somaclonal variations in Sipahutar pineapple seedlings generated from in vitro culture using Inter Simple Sequence Repeat (ISSR) markers. Fifteen accessions were used in this study consisting of Sipahutar pineapple grown in its original environment, a laboratory environment, acclimatized, and in vitro cultured. Total of 10 primers, of which seven primers detected polymorphisms among the population. This study resulted in a low genetic similarity among the Sipahutar pineapple population. The genetic diversity of Sipahutar pineapple plants propagated by in vitro culture had a similarity level of 75%-94%, showing somaclonal variations in vitro culture. The genetic similarity of pineapple accessions was divided into two main clusters, namely cluster A consisted of 11 accessions and cluster B consisted of 4 accessions. Cluster separation does not correlate to the source of plant materials studied.

Biosfer: Jurnal Tadris Biologi

This research employed the experimental method with descriptive data analysis. It aimed to isolat... more This research employed the experimental method with descriptive data analysis. It aimed to isolate and identify sponge symbiont bacteria with antibacterial potential against Multi-Drug Resistance Organisms (MDRO) with the 16S rRNA gene marker. Based on the study results, two sponges were obtained, namely Clathrina sp and Agelas sp, each having 11 and 7 isolates of symbiont bacteria, respectively. Based on the antibacterial activity test results, out of 18 isolates of symbiont bacteria, only 4 had antibacterial potential against MDRO (Klebsiella pneumoniae ESBL, Pseudomonas aeruginosa ESBL, or Staphylococcus lugdunensis MRSA) with inhibition zones of 9.3 mm, 8.3 mm, and 8.1 mm, respectively. The results of bacterial identification using the 16S rRNA gene sequence isolate S1I3, S1I9, and A1I3 belong to the Bacillus cereus and Bacillus paramycoides types. This research is expected to provide important information about sponge symbiont bacteria with antibacterial potential against MDRO.

International Journal of Emerging Technologies in Learning (iJET)

This study aims to analyze students' digital literacy in the context of learning biology mate... more This study aims to analyze students' digital literacy in the context of learning biology material on the digestive system and identify students' digital literacy in terms of interest in digital technology, internet costs, gender, and learning outcomes. The research was conducted at one private high school in Medan, Indonesia. The instrument used is a questionnaire and a list of student grades from the teacher. The results showed that students interested in digital technology had a significantly higher digital literacy score than students who had less interest in digital technology. There was no significant difference in digital literacy between groups of students with internet fees < US$ 14 per month and students with internet fees of US$ 14 per month, and between groups of male and female students. The result of the analysis also found a positive correlation between learning outcomes and digital literacy.

Berkala Penelitian Hayati

Lichen is a symbiotic organism consisting of algae (photobionts) and fungi (mycobiont). In Indone... more Lichen is a symbiotic organism consisting of algae (photobionts) and fungi (mycobiont). In Indonesia, Usnea is the only genus of lichen that has been widely recognized and used by the local community for traditional medicine. It is locally known as kayu angin, rusuk angin, or janggot Kai. Furthermore, it produces lichen acid and other compounds as secondary metabolites that need investigation. Therefore, this study aimed to identify species and determine the secondary metabolites of Usnea in West Java. There were 13 species of Usnea identified, with U. rubrotincta, U. mutabilis, and U. barbata being new to West Java. The extraction of U. esperantiana has the highest number of lichen acids, with 20 different types identified. This study showed that Usnea contains lichen acid, flavonoid, and saponin compounds. The secondary metabolites are a potential source of natural products for bioprospecting.

This data describes the results of research on "Genetic Stability Analysis Of In Vitro Pinea... more This data describes the results of research on "Genetic Stability Analysis Of In Vitro Pineapple From Sipahutar Using Inter Simple Sequence Repeat (ISSR) Molekular Marker"

Biogenesis: Jurnal Ilmiah Biologi, 2021

Xanthomonas oryzae pv. oryzae (Xoo) causes bacterial leaf blight on rice plants, which is respons... more Xanthomonas oryzae pv. oryzae (Xoo) causes bacterial leaf blight on rice plants, which is responsible for crop failure, resulting in economic losses. Control of Xoo bacteria can be accomplished through the use of Xoo-resistant rice varieties. Due to the shifting genetic structure and environmental conditions of Xoo bacteria, it is difficult to identify them according to their pathotype. This study aims to determine the genetic diversity of Xoo bacteria using the polymerase chain reaction (PCR) method and the IS1112-based RAPD marker, which is known to contain repetitive sequences. DNA was isolated from 52 isolates collected from 15 districts and cities in North Sumatra province and then used to amplify the IS1112 sequence. Dendrogram analysis revealed that cluster analysis of PCR findings classified isolates into 15 groups with a 90% similarity value. Genetic variation among Xoo isolates from North Sumatra ranged between 51% and 100%. North Sumatra Xoo isolates exhibited a high degr...

Journal of Physics: Conference Series, 2021

Melon (Cucumis melo L.) is a member of the Cucurbitaceae family spread throughout the world. Melo... more Melon (Cucumis melo L.) is a member of the Cucurbitaceae family spread throughout the world. Melons have very varied sexual types, which can be important information in genetic analysis and breeding. Sex type jumps from genetic factors, environmental factors, and environmental conditions. The aim of this study was the expression of CmACS-7 gene expression in melon flowers in various sexual types. This study used melons with andromonoecious sexual types (Melodi Gama 1 and Melodi Gama 3 cultivars) and monoecious (PI 371795 and Bartek). This study uses quantitative PCR analysis using real-time PCR with relative quantitative methods. RNA was isolated and then synthesized into cDNA, then amplified using specific primers. The number of cDNA specific genes was analyzed using Real-time PCR to calculate the level of gene expression. The results showed that the CmACS-7 gene was expressed more in female and hermaphrodite flowers than in male flowers. In male flowers with andromonoecious sexual...

Sipahutar pineapple is very popular in North Sumatra, because of the distinctive sweet taste and ... more Sipahutar pineapple is very popular in North Sumatra, because of the distinctive sweet taste and normal water content. Furthermore, it is large in size and has a yellow fruit skin color with greenish tips. However, the problem with Sipahutar pineapple production is the limited amount of good quality seeds. The utilization of in vitro culture techniques on pineapples allows the emergence of somaclonal variations, especially in plantlets that have undergone the subculture stage. This somaclonal variation is one of the problems in commercial seedling production, therefore, it is necessary to conduct an initial examination using the inter simple sequence repeat (ISSR) molecular marker. This study aimed to determine the genetic and primary stability of ISSR, which can be used in pineapple plants native to Sipahutar. The methods used include sample preparation, DNA isolation, primer optimization, ISSR primer amplification by PCR method, and electrophoresis. Furthermore, a total of 15 samp...

Andaliman (Zanthoxylum acanthopodium DC.) merupakan jenis dari famili Rutaceae yang tersebar luas... more Andaliman (Zanthoxylum acanthopodium DC.) merupakan jenis dari famili Rutaceae yang tersebar luas di wilayah Sumatera Utara, Indonesia. Andaliman digunakan dalam bumbu masakan khas tradisional suku Batak, Sumatera Utara. Andaliman memiliki daya dan persentase perkecambahan yang rendah serta waktu berkecambah yang panjang. Andaliman termasuk tanaman endemik kawasan sekitar danau Toba yang memiliki prioritas untuk dikonservasi dalam program Global Network of National Geoparks kaldera Toba oleh UNESCO. Andaliman hanya dapat tumbuh dengan baik jika berada di habitat aslinya. Berkurangnya luas hutan di Sumatera Utara akibat illegal logging serta perubahan fungsi hutan menjadi hutan produksi dan berkebunan berperan serta mengancam eksistensi andaliman di Sumatera Utara. Berdasarkan karakteristik habitat andaliman, strategi konservasi yang sesuai adalah konservasi in situ. Penelitian ini bertujuan untuk menganalisis wilayah konservasi in situ menggunakan analisis spasial geografi dan maxim...

Journal of Physics: Conference Series, 2019

Andaliman (Zanthoxylum acanthopodium DC) is a member of the Rutaceae family which is spread in th... more Andaliman (Zanthoxylum acanthopodium DC) is a member of the Rutaceae family which is spread in the regions of East Asia, Southeast Asia, and South Asia. In the territory of Indonesia, andaliman is spread in the highlands of North Sumatra and parts of Aceh. Andaliman is a spice plant that is usually used as a spice for the Batak tribe in North Sumatra. This study aims to analyze the characteristics of andaliman habitat in North Sumatra. A total of 169 samples from 8 regencies in North Sumatra analyzed habitat characteristics using the ArcGis 10.3 program using various ecological data. The results showed that andaliman was widely distributed in the highlands in North Sumatra. Andaliman inhabits 4 soil types, namely Humic Acrisols (36.09%), Orthic Acrisols (34.31%), Humic Cambisols (18.34%), and Orthic Ferralsols (11.24%), 7 Types of land cover namely dry land agriculture (81.06%), open soil (0.59%), rice fields (6.5%), settlements (2.9%), secondary dryland forests (4.73%), shrubs (3.5...

Abstract. Harsono T, Pasaribu N, Sobir, Fitmawati, Prasetya E. 2017. Phylogenetic analysis of Ind... more Abstract. Harsono T, Pasaribu N, Sobir, Fitmawati, Prasetya E. 2017. Phylogenetic analysis of Indonesian gandaria (Bouea) using molecular markers of cpDNA . Genus Bouea is a member of the Family Anacardiaceae which is widespread in Malesia region. Genus Bouea consists of two types, namely, B. oppositifolia (Roxb.) Adelb. and B. macrophylla Griffit. This study aims to uncover the genetic diversity of Bouea in Indonesia based on molecular markers of cpDNA trnL-F intergenic spacer. The samples of genetic material analyzed are 7 accessions of B. oppositifolia and 8 accessions of B. macrophylla coming from the collection of the Bogor Botanical Gardens and the field exploration activity in Sumatra, Java, Kalimantan and Ambon, and Anacardium occidentale and Mangifera indica are also used as an out-group comparator. Based on phylogenetic analysis using the maximum parsimony and neighbor-joining method, genus Bouea is a monophyletic group or is derived from the same ancestor. Bouea derived f...

European Journal of Experimental Biology, 2015

The bark of raru (C. melanoxylon) has been utilized by local community as antidiarrheal medicine ... more The bark of raru (C. melanoxylon) has been utilized by local community as antidiarrheal medicine because of the content of active antibacterial compounds. The antibacterial activity of the bark of raru is induced by endophytic bacteria. The objectives of this study are to obtain, and elucidate and the role of endophytic bacteria in tissues of the bark of raru. The presence of endophytic bacteria in tissues of the bark of raru (5-10 years old) was observed microscopically using the slice of technique histological tissues, and the bacteria were directly isolated from the tissues by planting pour plate on Tryptic Soya Agar (TSA) medium. The antibacterial activity of endophytic bacteria isolates was examined using a dual cultural assay against Escherichia coli ATCC 35218 and Staphyllococcus aureus ATCC 25923 as a model bacterial. The characterization of endophytic bacteria isolates as the antibacterial synthetic inducer of the bark of raru was carry out through a cultivation experiment ...

Journal of Physics: Conference Series

The indicator of water pollution according to microbiological standards is the presence of microb... more The indicator of water pollution according to microbiological standards is the presence of microbiological pollutants. To see the presence of bacteria in the waters, it is necessary to study the isolation and characterization of these bacteria. The existence of the river in the Mandailing Natal area has experienced a change in character due to gold mining activities along the river flow. The purpose of this study was to determine the types of bacteria in the river in the Mandailing Natal area of North Sumatra. This research was conducted at the Laboratory of Microbiology, Department of Biology, Faculty of Mathematics and Natural Sciences, State University of Medan. The research period was from June to October 2020. The population in this study was the water of the Batang Gadis Mandailing Natal river, North Sumatra. The sample in this study is the Batang Gadis river water around the Mandailing Natal gold mine, North Sumatra. The research was carried out by taking samples and isolatin...

Anaphalis longifolia merupakan anggota dari family Asteraceae yang tersebar di dataran tinggi Ero... more Anaphalis longifolia merupakan anggota dari family Asteraceae yang tersebar di dataran tinggi Eropa, Amerika, hingga Asia. Penelitian tentang tanaman ini masih terbatas pada studi habitat, sedangkan penelitian terkait identifikasi molekuler masih belum dilakukan. Penelitian ini bertujuan untuk menganalisis DNA barcode dari A. longifolia menggunakan sekuen mat K gene. Sampel yang diperoleh dari Sumatera Utara kemudian di Isolasi DNA, di amplifikasi menggunakan primer spesifik, lalu disequencing. Hasil sequencing dianalisis menggunakan program Molecular Evolution Genetics Analysis (MEGA) Version X. Hasil penelitian menunjukkan bahwa sekuen mat K gen berhasil diamplifikasi pada panjang 800-850 kb. Hasil analisis pohon filogenetik menunjukkan bahwa sekuen mat K gene dapat mengelompokkan A. longifolia . Pada sekuen mat K gene A. longifolia , AT content lebih tinggi dibandingkan dengan GC conten. Jarak genetik yang diperoleh berkisar 0-0.0014. Hasil analisis alignment sekuen mat K gene me...

Sitompul FA, Siregar EH, Roesma DI, Dahelmi, Prasetya E. 2018. Molecular identification of coffee... more Sitompul FA, Siregar EH, Roesma DI, Dahelmi, Prasetya E. 2018. Molecular identification of coffee (Coffea arabica) pollinator insects in North Sumatra, Indonesia based on designed COI primers. Biodiversitas 19: 1877-1883. Coffee (Coffea arabica L.) is one of the most important economic commodities in the province of North Sumatra, Indonesia. Insects associated with pollination of C. arabica are one of the key factors for successful cultivation of C. arabica, but, the research regarding of these was still limited. The population of coffee plant is scattered across the highlands of Indonesia and the pollination of C. arabica is strongly believed linked to a diverse group of pollinating insects. However, lack of taxonomic identification of insects pollinating these plants has become one of constraints to succeed the cultivation of C. Arabica. This study aimed to analyze types and variations of pollinating insects of C. arabica in the province of North Sumatra, Indonesia, using DNA barc...

The resistance of pathogenic bacteria to antibiotics is increasing due to antibiotics with incorr... more The resistance of pathogenic bacteria to antibiotics is increasing due to antibiotics with incorrect doses, wrong diagnostics, and the wrong target. Bacteria that have been resistant to several antibiotics are called multi-drug resistant organisms (MDRO) bacteria. Bacterial resistance to some antibiotics requires alternative herbal treatments, one of which is the Chromolaena odorata L. Research must therefore be conducted on the antibacterial activity of the ethanol extract fraction of C. odorata L. leaves for MDRO bacteria, such as Staphylococcus lugdunensis methicillin-resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa extended-spectrum beta-lactamase (ESBL), and Klebsiella pneumoniae ESBL. This study aims to determine the antibacterial activity of the ethanol extract of Kirinyuh leaves (C. odorata L.) and the antibacterial activity of the fractionation against MDRO bacteria. Test results of kirinyuh leaf ethanol extract for S. lugdunensis MRSA, P. aeruginosa ESBL, and...

Tropical Life Sciences Research

Andaliman (Zanthoxylum acanthopodium DC) is a native plant of North Sumatra province. Zanthoxylum... more Andaliman (Zanthoxylum acanthopodium DC) is a native plant of North Sumatra province. Zanthoxylum acanthopodium is a member of Rutaceae family widely found in northern Sumatra, Indonesia. The aim of this study was to barcode Z. acanthopodium in North Sumatra province, Indonesia based on cpDNA maturase K (matK). Samples were collected in seven localities across six regions of North Sumatra province. Phylogenetic analysis was conducted using Maximum Likelihood method. The results of phylogenetic analysis indicate that Z. acanthopodium is a monophyletic group that is derived from a common ancestor. The results of the phylogenetic tree construction show that there is a grouping of accession between Z. acanthopodium species separate from other species in the Zanthoxylum genus as well as those of the Rutaceae family. The results showed that cpDNA matK marker can effectively be used as DNA barcoding to identify Z. acanthopodium.

JURNAL BIOSAINS

From a Muslim perspective, it is very important to know the content, raw materials, and processin... more From a Muslim perspective, it is very important to know the content, raw materials, and processing of the raw materials used in the cosmetic products used. One type of cosmetics that is most often used is lipstick. However, many lipsticks circulating in Indonesia are not equipped with a halal logo. One of the ingredients for lipstick is pork derivatives. These pig derivatives can be detected using PCR. Based on this background, this study aims to test the presence of pig DNA in lipstick samples that have not been certified halal on the market using 4 combinations of pig DNA fragments coding primers by using the conventional PCR method. Five commercial lipstick samples were selected by purposive sampling. DNA isolation was carried out according to the Wizard Promega Universal Kit. The PCR process was carried out with temperature optimization as follows: Predenaturation 98oC: 2 minutes, denaturation of 95oC: 30 seconds, Annealing 61oC: 30 seconds, Extension 72oC: 40 seconds, and Poste...

Proceedings of the Proceedings of The 5th Annual International Seminar on Trends in Science and Science Education, AISTSSE 2018, 18-19 October 2018, Medan, Indonesia, 2019

Dendrocygna javanica (Horsfield, 1821) is a member of the family Anatidae, the order of Anserifor... more Dendrocygna javanica (Horsfield, 1821) is a member of the family Anatidae, the order of Anseriformes, class of Aves, phylum Chordata, of kingdom Animalia. This species is commonly called the Lesser Whistling-duck because it flies in a noisy sound. In Indonesia, it is known as Belibis Batu. Dendrocygna javanica is spread in the regions of Asia including Indonesia. In Indonesia, D. javanica is spread on the islands of Sumatra, Kalimantan, Java, East Nusa Tenggara and West Nusa Tenggara. This study aims to create a distribution model of Dendrocygna javanica based on habitat characteristics in North Sumatra, Indonesia, using GIS (Geographic Information System). A total of 35 distribution points of D. javanica in 8 districts in North Sumatra were analyzed using ArcGis 10.3 software to analyze habitat characteristics. The results showed that D. javanica spread on habitat characteristics Rainfall ranged from 1500-3500 mm/year, 5 soil types namely ferric acrisols (4 points), humic acrisols (4 points), dystric cambisols (2 points), orthic ferrasols (5 point), and dystric fluvisols (20 points), 7 types of land cover namely dryland forest (2 points), plantation (5 points), dryland agriculture (10 points), rice fields (2 points), swamp (4 points), plus (4 points), and body of water (8 points), and are at an elevation of 3-1072 meters above sea level. Dendrocygna javanica is spread in a limited area. This research is expected to be an important source of information in the conservation of D. javanica in North Sumatra

Proceedings of the Proceedings of The 5th Annual International Seminar on Trends in Science and Science Education, AISTSSE 2018, 18-19 October 2018, Medan, Indonesia, 2019

Endophytic microbes are microorganisms that colonize the parts of plant organs and interact with ... more Endophytic microbes are microorganisms that colonize the parts of plant organs and interact with host plant without causing any symptoms or diseases in the host plant. Endophytic microbial colonization is found in various types of plant organs, namely: roots, stems, leaves and seeds. This study is focused on endophytic bacteria mostly conducted intensively on wild plants or forests in the tropical ecosystems. Various trees or tropical forest plants have potentials as traditional medicine and in cooperation with the bacteria. The C. melanoxylon has been utilized by local community as antidiarrheal medicine because of the content of active antibacterial compounds. The presence of endophytic bacteria in tissues of the stem of C. melanoxylon was observed the bacteria were directly isolated from the tissues by planting pour plate on Tryptic Soya Agar (TSA) medium. The antibacterial activity of endophytic bacteria isolates was examined using a dual cultural assay against Escherichia coli ATCC 35218 and Staphyllococcus aureus ATCC 25923 as a model bacterial. Morphological, physiological, and biochemical characters were identified based on the ability of the isolates to grow. The endophytic bacteria isolate of strain ER-2 was inhibitat the growth of E. coli ATCC 35218 and S. aureus ATCC 25923.

Biodiversitas Journal of Biological Diversity

Sipahutar pineapple from North Sumatra, Indonesia, is considered difficult to breed, resulting in... more Sipahutar pineapple from North Sumatra, Indonesia, is considered difficult to breed, resulting in a lack of seed availability. However, tissue culture allows the emergence of somaclonal variations, especially in plantlets undergoing several subculture stages. This study aimed to identify somaclonal variations in Sipahutar pineapple seedlings generated from in vitro culture using Inter Simple Sequence Repeat (ISSR) markers. Fifteen accessions were used in this study consisting of Sipahutar pineapple grown in its original environment, a laboratory environment, acclimatized, and in vitro cultured. Total of 10 primers, of which seven primers detected polymorphisms among the population. This study resulted in a low genetic similarity among the Sipahutar pineapple population. The genetic diversity of Sipahutar pineapple plants propagated by in vitro culture had a similarity level of 75%-94%, showing somaclonal variations in vitro culture. The genetic similarity of pineapple accessions was divided into two main clusters, namely cluster A consisted of 11 accessions and cluster B consisted of 4 accessions. Cluster separation does not correlate to the source of plant materials studied.

Biosfer: Jurnal Tadris Biologi

This research employed the experimental method with descriptive data analysis. It aimed to isolat... more This research employed the experimental method with descriptive data analysis. It aimed to isolate and identify sponge symbiont bacteria with antibacterial potential against Multi-Drug Resistance Organisms (MDRO) with the 16S rRNA gene marker. Based on the study results, two sponges were obtained, namely Clathrina sp and Agelas sp, each having 11 and 7 isolates of symbiont bacteria, respectively. Based on the antibacterial activity test results, out of 18 isolates of symbiont bacteria, only 4 had antibacterial potential against MDRO (Klebsiella pneumoniae ESBL, Pseudomonas aeruginosa ESBL, or Staphylococcus lugdunensis MRSA) with inhibition zones of 9.3 mm, 8.3 mm, and 8.1 mm, respectively. The results of bacterial identification using the 16S rRNA gene sequence isolate S1I3, S1I9, and A1I3 belong to the Bacillus cereus and Bacillus paramycoides types. This research is expected to provide important information about sponge symbiont bacteria with antibacterial potential against MDRO.

International Journal of Emerging Technologies in Learning (iJET)

This study aims to analyze students' digital literacy in the context of learning biology mate... more This study aims to analyze students' digital literacy in the context of learning biology material on the digestive system and identify students' digital literacy in terms of interest in digital technology, internet costs, gender, and learning outcomes. The research was conducted at one private high school in Medan, Indonesia. The instrument used is a questionnaire and a list of student grades from the teacher. The results showed that students interested in digital technology had a significantly higher digital literacy score than students who had less interest in digital technology. There was no significant difference in digital literacy between groups of students with internet fees < US$ 14 per month and students with internet fees of US$ 14 per month, and between groups of male and female students. The result of the analysis also found a positive correlation between learning outcomes and digital literacy.

Berkala Penelitian Hayati

Lichen is a symbiotic organism consisting of algae (photobionts) and fungi (mycobiont). In Indone... more Lichen is a symbiotic organism consisting of algae (photobionts) and fungi (mycobiont). In Indonesia, Usnea is the only genus of lichen that has been widely recognized and used by the local community for traditional medicine. It is locally known as kayu angin, rusuk angin, or janggot Kai. Furthermore, it produces lichen acid and other compounds as secondary metabolites that need investigation. Therefore, this study aimed to identify species and determine the secondary metabolites of Usnea in West Java. There were 13 species of Usnea identified, with U. rubrotincta, U. mutabilis, and U. barbata being new to West Java. The extraction of U. esperantiana has the highest number of lichen acids, with 20 different types identified. This study showed that Usnea contains lichen acid, flavonoid, and saponin compounds. The secondary metabolites are a potential source of natural products for bioprospecting.

This data describes the results of research on "Genetic Stability Analysis Of In Vitro Pinea... more This data describes the results of research on "Genetic Stability Analysis Of In Vitro Pineapple From Sipahutar Using Inter Simple Sequence Repeat (ISSR) Molekular Marker"

Biogenesis: Jurnal Ilmiah Biologi, 2021

Xanthomonas oryzae pv. oryzae (Xoo) causes bacterial leaf blight on rice plants, which is respons... more Xanthomonas oryzae pv. oryzae (Xoo) causes bacterial leaf blight on rice plants, which is responsible for crop failure, resulting in economic losses. Control of Xoo bacteria can be accomplished through the use of Xoo-resistant rice varieties. Due to the shifting genetic structure and environmental conditions of Xoo bacteria, it is difficult to identify them according to their pathotype. This study aims to determine the genetic diversity of Xoo bacteria using the polymerase chain reaction (PCR) method and the IS1112-based RAPD marker, which is known to contain repetitive sequences. DNA was isolated from 52 isolates collected from 15 districts and cities in North Sumatra province and then used to amplify the IS1112 sequence. Dendrogram analysis revealed that cluster analysis of PCR findings classified isolates into 15 groups with a 90% similarity value. Genetic variation among Xoo isolates from North Sumatra ranged between 51% and 100%. North Sumatra Xoo isolates exhibited a high degr...

Journal of Physics: Conference Series, 2021

Melon (Cucumis melo L.) is a member of the Cucurbitaceae family spread throughout the world. Melo... more Melon (Cucumis melo L.) is a member of the Cucurbitaceae family spread throughout the world. Melons have very varied sexual types, which can be important information in genetic analysis and breeding. Sex type jumps from genetic factors, environmental factors, and environmental conditions. The aim of this study was the expression of CmACS-7 gene expression in melon flowers in various sexual types. This study used melons with andromonoecious sexual types (Melodi Gama 1 and Melodi Gama 3 cultivars) and monoecious (PI 371795 and Bartek). This study uses quantitative PCR analysis using real-time PCR with relative quantitative methods. RNA was isolated and then synthesized into cDNA, then amplified using specific primers. The number of cDNA specific genes was analyzed using Real-time PCR to calculate the level of gene expression. The results showed that the CmACS-7 gene was expressed more in female and hermaphrodite flowers than in male flowers. In male flowers with andromonoecious sexual...

Sipahutar pineapple is very popular in North Sumatra, because of the distinctive sweet taste and ... more Sipahutar pineapple is very popular in North Sumatra, because of the distinctive sweet taste and normal water content. Furthermore, it is large in size and has a yellow fruit skin color with greenish tips. However, the problem with Sipahutar pineapple production is the limited amount of good quality seeds. The utilization of in vitro culture techniques on pineapples allows the emergence of somaclonal variations, especially in plantlets that have undergone the subculture stage. This somaclonal variation is one of the problems in commercial seedling production, therefore, it is necessary to conduct an initial examination using the inter simple sequence repeat (ISSR) molecular marker. This study aimed to determine the genetic and primary stability of ISSR, which can be used in pineapple plants native to Sipahutar. The methods used include sample preparation, DNA isolation, primer optimization, ISSR primer amplification by PCR method, and electrophoresis. Furthermore, a total of 15 samp...

Andaliman (Zanthoxylum acanthopodium DC.) merupakan jenis dari famili Rutaceae yang tersebar luas... more Andaliman (Zanthoxylum acanthopodium DC.) merupakan jenis dari famili Rutaceae yang tersebar luas di wilayah Sumatera Utara, Indonesia. Andaliman digunakan dalam bumbu masakan khas tradisional suku Batak, Sumatera Utara. Andaliman memiliki daya dan persentase perkecambahan yang rendah serta waktu berkecambah yang panjang. Andaliman termasuk tanaman endemik kawasan sekitar danau Toba yang memiliki prioritas untuk dikonservasi dalam program Global Network of National Geoparks kaldera Toba oleh UNESCO. Andaliman hanya dapat tumbuh dengan baik jika berada di habitat aslinya. Berkurangnya luas hutan di Sumatera Utara akibat illegal logging serta perubahan fungsi hutan menjadi hutan produksi dan berkebunan berperan serta mengancam eksistensi andaliman di Sumatera Utara. Berdasarkan karakteristik habitat andaliman, strategi konservasi yang sesuai adalah konservasi in situ. Penelitian ini bertujuan untuk menganalisis wilayah konservasi in situ menggunakan analisis spasial geografi dan maxim...

Journal of Physics: Conference Series, 2019

Andaliman (Zanthoxylum acanthopodium DC) is a member of the Rutaceae family which is spread in th... more Andaliman (Zanthoxylum acanthopodium DC) is a member of the Rutaceae family which is spread in the regions of East Asia, Southeast Asia, and South Asia. In the territory of Indonesia, andaliman is spread in the highlands of North Sumatra and parts of Aceh. Andaliman is a spice plant that is usually used as a spice for the Batak tribe in North Sumatra. This study aims to analyze the characteristics of andaliman habitat in North Sumatra. A total of 169 samples from 8 regencies in North Sumatra analyzed habitat characteristics using the ArcGis 10.3 program using various ecological data. The results showed that andaliman was widely distributed in the highlands in North Sumatra. Andaliman inhabits 4 soil types, namely Humic Acrisols (36.09%), Orthic Acrisols (34.31%), Humic Cambisols (18.34%), and Orthic Ferralsols (11.24%), 7 Types of land cover namely dry land agriculture (81.06%), open soil (0.59%), rice fields (6.5%), settlements (2.9%), secondary dryland forests (4.73%), shrubs (3.5...

Abstract. Harsono T, Pasaribu N, Sobir, Fitmawati, Prasetya E. 2017. Phylogenetic analysis of Ind... more Abstract. Harsono T, Pasaribu N, Sobir, Fitmawati, Prasetya E. 2017. Phylogenetic analysis of Indonesian gandaria (Bouea) using molecular markers of cpDNA . Genus Bouea is a member of the Family Anacardiaceae which is widespread in Malesia region. Genus Bouea consists of two types, namely, B. oppositifolia (Roxb.) Adelb. and B. macrophylla Griffit. This study aims to uncover the genetic diversity of Bouea in Indonesia based on molecular markers of cpDNA trnL-F intergenic spacer. The samples of genetic material analyzed are 7 accessions of B. oppositifolia and 8 accessions of B. macrophylla coming from the collection of the Bogor Botanical Gardens and the field exploration activity in Sumatra, Java, Kalimantan and Ambon, and Anacardium occidentale and Mangifera indica are also used as an out-group comparator. Based on phylogenetic analysis using the maximum parsimony and neighbor-joining method, genus Bouea is a monophyletic group or is derived from the same ancestor. Bouea derived f...

European Journal of Experimental Biology, 2015

The bark of raru (C. melanoxylon) has been utilized by local community as antidiarrheal medicine ... more The bark of raru (C. melanoxylon) has been utilized by local community as antidiarrheal medicine because of the content of active antibacterial compounds. The antibacterial activity of the bark of raru is induced by endophytic bacteria. The objectives of this study are to obtain, and elucidate and the role of endophytic bacteria in tissues of the bark of raru. The presence of endophytic bacteria in tissues of the bark of raru (5-10 years old) was observed microscopically using the slice of technique histological tissues, and the bacteria were directly isolated from the tissues by planting pour plate on Tryptic Soya Agar (TSA) medium. The antibacterial activity of endophytic bacteria isolates was examined using a dual cultural assay against Escherichia coli ATCC 35218 and Staphyllococcus aureus ATCC 25923 as a model bacterial. The characterization of endophytic bacteria isolates as the antibacterial synthetic inducer of the bark of raru was carry out through a cultivation experiment ...

Journal of Physics: Conference Series

The indicator of water pollution according to microbiological standards is the presence of microb... more The indicator of water pollution according to microbiological standards is the presence of microbiological pollutants. To see the presence of bacteria in the waters, it is necessary to study the isolation and characterization of these bacteria. The existence of the river in the Mandailing Natal area has experienced a change in character due to gold mining activities along the river flow. The purpose of this study was to determine the types of bacteria in the river in the Mandailing Natal area of North Sumatra. This research was conducted at the Laboratory of Microbiology, Department of Biology, Faculty of Mathematics and Natural Sciences, State University of Medan. The research period was from June to October 2020. The population in this study was the water of the Batang Gadis Mandailing Natal river, North Sumatra. The sample in this study is the Batang Gadis river water around the Mandailing Natal gold mine, North Sumatra. The research was carried out by taking samples and isolatin...

Anaphalis longifolia merupakan anggota dari family Asteraceae yang tersebar di dataran tinggi Ero... more Anaphalis longifolia merupakan anggota dari family Asteraceae yang tersebar di dataran tinggi Eropa, Amerika, hingga Asia. Penelitian tentang tanaman ini masih terbatas pada studi habitat, sedangkan penelitian terkait identifikasi molekuler masih belum dilakukan. Penelitian ini bertujuan untuk menganalisis DNA barcode dari A. longifolia menggunakan sekuen mat K gene. Sampel yang diperoleh dari Sumatera Utara kemudian di Isolasi DNA, di amplifikasi menggunakan primer spesifik, lalu disequencing. Hasil sequencing dianalisis menggunakan program Molecular Evolution Genetics Analysis (MEGA) Version X. Hasil penelitian menunjukkan bahwa sekuen mat K gen berhasil diamplifikasi pada panjang 800-850 kb. Hasil analisis pohon filogenetik menunjukkan bahwa sekuen mat K gene dapat mengelompokkan A. longifolia . Pada sekuen mat K gene A. longifolia , AT content lebih tinggi dibandingkan dengan GC conten. Jarak genetik yang diperoleh berkisar 0-0.0014. Hasil analisis alignment sekuen mat K gene me...

Sitompul FA, Siregar EH, Roesma DI, Dahelmi, Prasetya E. 2018. Molecular identification of coffee... more Sitompul FA, Siregar EH, Roesma DI, Dahelmi, Prasetya E. 2018. Molecular identification of coffee (Coffea arabica) pollinator insects in North Sumatra, Indonesia based on designed COI primers. Biodiversitas 19: 1877-1883. Coffee (Coffea arabica L.) is one of the most important economic commodities in the province of North Sumatra, Indonesia. Insects associated with pollination of C. arabica are one of the key factors for successful cultivation of C. arabica, but, the research regarding of these was still limited. The population of coffee plant is scattered across the highlands of Indonesia and the pollination of C. arabica is strongly believed linked to a diverse group of pollinating insects. However, lack of taxonomic identification of insects pollinating these plants has become one of constraints to succeed the cultivation of C. Arabica. This study aimed to analyze types and variations of pollinating insects of C. arabica in the province of North Sumatra, Indonesia, using DNA barc...

The resistance of pathogenic bacteria to antibiotics is increasing due to antibiotics with incorr... more The resistance of pathogenic bacteria to antibiotics is increasing due to antibiotics with incorrect doses, wrong diagnostics, and the wrong target. Bacteria that have been resistant to several antibiotics are called multi-drug resistant organisms (MDRO) bacteria. Bacterial resistance to some antibiotics requires alternative herbal treatments, one of which is the Chromolaena odorata L. Research must therefore be conducted on the antibacterial activity of the ethanol extract fraction of C. odorata L. leaves for MDRO bacteria, such as Staphylococcus lugdunensis methicillin-resistant Staphylococcus aureus (MRSA), Pseudomonas aeruginosa extended-spectrum beta-lactamase (ESBL), and Klebsiella pneumoniae ESBL. This study aims to determine the antibacterial activity of the ethanol extract of Kirinyuh leaves (C. odorata L.) and the antibacterial activity of the fractionation against MDRO bacteria. Test results of kirinyuh leaf ethanol extract for S. lugdunensis MRSA, P. aeruginosa ESBL, and...

Tropical Life Sciences Research

Andaliman (Zanthoxylum acanthopodium DC) is a native plant of North Sumatra province. Zanthoxylum... more Andaliman (Zanthoxylum acanthopodium DC) is a native plant of North Sumatra province. Zanthoxylum acanthopodium is a member of Rutaceae family widely found in northern Sumatra, Indonesia. The aim of this study was to barcode Z. acanthopodium in North Sumatra province, Indonesia based on cpDNA maturase K (matK). Samples were collected in seven localities across six regions of North Sumatra province. Phylogenetic analysis was conducted using Maximum Likelihood method. The results of phylogenetic analysis indicate that Z. acanthopodium is a monophyletic group that is derived from a common ancestor. The results of the phylogenetic tree construction show that there is a grouping of accession between Z. acanthopodium species separate from other species in the Zanthoxylum genus as well as those of the Rutaceae family. The results showed that cpDNA matK marker can effectively be used as DNA barcoding to identify Z. acanthopodium.

JURNAL BIOSAINS

From a Muslim perspective, it is very important to know the content, raw materials, and processin... more From a Muslim perspective, it is very important to know the content, raw materials, and processing of the raw materials used in the cosmetic products used. One type of cosmetics that is most often used is lipstick. However, many lipsticks circulating in Indonesia are not equipped with a halal logo. One of the ingredients for lipstick is pork derivatives. These pig derivatives can be detected using PCR. Based on this background, this study aims to test the presence of pig DNA in lipstick samples that have not been certified halal on the market using 4 combinations of pig DNA fragments coding primers by using the conventional PCR method. Five commercial lipstick samples were selected by purposive sampling. DNA isolation was carried out according to the Wizard Promega Universal Kit. The PCR process was carried out with temperature optimization as follows: Predenaturation 98oC: 2 minutes, denaturation of 95oC: 30 seconds, Annealing 61oC: 30 seconds, Extension 72oC: 40 seconds, and Poste...

Proceedings of the Proceedings of The 5th Annual International Seminar on Trends in Science and Science Education, AISTSSE 2018, 18-19 October 2018, Medan, Indonesia, 2019

Dendrocygna javanica (Horsfield, 1821) is a member of the family Anatidae, the order of Anserifor... more Dendrocygna javanica (Horsfield, 1821) is a member of the family Anatidae, the order of Anseriformes, class of Aves, phylum Chordata, of kingdom Animalia. This species is commonly called the Lesser Whistling-duck because it flies in a noisy sound. In Indonesia, it is known as Belibis Batu. Dendrocygna javanica is spread in the regions of Asia including Indonesia. In Indonesia, D. javanica is spread on the islands of Sumatra, Kalimantan, Java, East Nusa Tenggara and West Nusa Tenggara. This study aims to create a distribution model of Dendrocygna javanica based on habitat characteristics in North Sumatra, Indonesia, using GIS (Geographic Information System). A total of 35 distribution points of D. javanica in 8 districts in North Sumatra were analyzed using ArcGis 10.3 software to analyze habitat characteristics. The results showed that D. javanica spread on habitat characteristics Rainfall ranged from 1500-3500 mm/year, 5 soil types namely ferric acrisols (4 points), humic acrisols (4 points), dystric cambisols (2 points), orthic ferrasols (5 point), and dystric fluvisols (20 points), 7 types of land cover namely dryland forest (2 points), plantation (5 points), dryland agriculture (10 points), rice fields (2 points), swamp (4 points), plus (4 points), and body of water (8 points), and are at an elevation of 3-1072 meters above sea level. Dendrocygna javanica is spread in a limited area. This research is expected to be an important source of information in the conservation of D. javanica in North Sumatra

Proceedings of the Proceedings of The 5th Annual International Seminar on Trends in Science and Science Education, AISTSSE 2018, 18-19 October 2018, Medan, Indonesia, 2019

Endophytic microbes are microorganisms that colonize the parts of plant organs and interact with ... more Endophytic microbes are microorganisms that colonize the parts of plant organs and interact with host plant without causing any symptoms or diseases in the host plant. Endophytic microbial colonization is found in various types of plant organs, namely: roots, stems, leaves and seeds. This study is focused on endophytic bacteria mostly conducted intensively on wild plants or forests in the tropical ecosystems. Various trees or tropical forest plants have potentials as traditional medicine and in cooperation with the bacteria. The C. melanoxylon has been utilized by local community as antidiarrheal medicine because of the content of active antibacterial compounds. The presence of endophytic bacteria in tissues of the stem of C. melanoxylon was observed the bacteria were directly isolated from the tissues by planting pour plate on Tryptic Soya Agar (TSA) medium. The antibacterial activity of endophytic bacteria isolates was examined using a dual cultural assay against Escherichia coli ATCC 35218 and Staphyllococcus aureus ATCC 25923 as a model bacterial. Morphological, physiological, and biochemical characters were identified based on the ability of the isolates to grow. The endophytic bacteria isolate of strain ER-2 was inhibitat the growth of E. coli ATCC 35218 and S. aureus ATCC 25923.

Bouea merupakan anggota dari famili Anacardiaceae yang terdistribusi luas di daerah Malesia. Male... more Bouea merupakan anggota dari famili Anacardiaceae yang terdistribusi luas di daerah Malesia. Malesia merupakan kawasan yang terbentang antara benua Asia dan Australia mencakup Filipina, Brunei, Papua Nugini, Indonesia, Malaysia, dan Singapura. Tanaman ini memiliki variasi fenotip karena keragaman genetik dan adaptasi di berbagai ragam kondisi lingkungan. Tujuan dari penelitian ini adalah mengklasifikasikan Bouea macrophylla Griffit berdasarkan variasi genetik menggunakan DNA kloroplas. Daerah TrnL-F intergenic spacer merupakan daerah nonkoding pada DNA kloroplas yang dapat digunakan sebagai penanda untuk melihat variasi genetik intraspesifik. Sampel diperoleh dari 5 tempat di Indonesia yaitu Ambon, Banten, Aceh, Sumatera Barat, dan Kalimantan Barat. Isolasi DNA menggunakan metode Doyle dan Doyle yang dimodifikasi, kemudian diamplifikasi menggunakan primer trnL-F forward dan primer trnL-F reverse. Produk PCR dengan panjang fragmen 350-400 bp kemudian disekuensing di 1st Base Singapura. Data hasil sekuensing dianalisis menggunakan software MEGA 6.06 untuk membangun pohon filogenetik menggunakan out group Mangifera indica dan Anacardium occidentale. Hasil dari peneltian ini menunjukkan bahwa daerah trnL-F pada DNA kloroplas adalah daerah dengan konservatif yang tinggi. Bouea macrophylla Griffit memiliki kedekatan dengan Anacardium occidentael. Analisis pohon filogenetik dengan menggunakan Software Mega 6.06 menunjukkan bahwa konsistensi spesies tersebut dari nenek moyang yang sama dan memisah dari spesies Mangifera indica dan Anacaardium occidentale sebagai out group.