Marco Chaud | University Of Sorocaba - UNISO (original) (raw)

Papers by Marco Chaud

Carbohydrate Polymer Technologies and Applications, 2021

Ars Pharmaceutica (Internet), 2019

Objectives: The physicochemical characteristics of bran of cassava starch flour and bran of cassa... more Objectives: The physicochemical characteristics of bran of cassava starch flour and bran of cassava flour (viz. organoleptic characteristics, pH value, moisture content, total ashes, lipid, protein, starch and fiber contents) and biopharmacotechnical parameters (viz. granulometry, flow capacity, angle at rest, outflow time and apparent density) were evaluated aiming at assessing their potential use as tablet excipients. Methodos: Three tablet formulations of venlafaxine hydrochloride were proposed, having as excipients bran of cassava flour, bran of cassava starch flour and Starch 1500®. The tablets were produced using two different pressures (98±5 MPa and 32±6 Mpa) and their mechanical (hardness and friability) and dissolubility characteristics were evaluated. Results and Conclusions: The tablets produced with both cassava flours, using higher pressures, presented similar physicochemical characteristics to those obtained with the excipient Starch1500®, thus indicating that cassava ...

Journal of Physics: Conference Series, 2019

Medicinal plant extracts are mostly used in different kinds of products in different areas, such ... more Medicinal plant extracts are mostly used in different kinds of products in different areas, such as pharmaceutical, cosmetic, food and veterinary, being employed in different dosages and applications. It is known that the metabolism of the human body is regulated by the presence or absence of certain chemical elements. Some elements enter vital functions, such as potassium and calcium. Potassium helps in muscle contractions, especially of the heart muscle, and calcium enters as an specific element in the bone composition. Some other elements like heavy metals (As, Hg, Cd, and Pb) are highly toxic to the human body, even at quite low concentrations. Therefore, there is the need to investigate the chemical composition of plants and medicinal extracts, because the presence of some elements in levels concentrations, as well as the deficiency of others, can lead to a series of metabolic disorders in the human body. The main objective of this study was to evaluate the chemical composition...

Journal of Drug Delivery Science and Technology, 2020

International Journal of Pharmaceutics, 2014

Solid lipid nanoparticles (SLN) are a promising drug delivery system for oral administration of p... more Solid lipid nanoparticles (SLN) are a promising drug delivery system for oral administration of poorly-water soluble drugs because of their capacity to increase the solubility of drug molecules when loaded in their lipid matrices, with the resulting improvement of the drug bioavailability. In the present work, we have developed praziquantel (PZQ)-loaded SLN and explored the biological applications of this system for intestinal permeation of PZQ. The effect in vitro on Schistosoma mansoni culture and the cytotoxicity in HepG2 line cell were also evaluated. The results showed a significant decrease in the intestinal absorption of PZQ loaded in SLN compared to free PZQ, suggesting that the SLN matrix could act as reservoir system. In culture of S. mansoni, we observed that PZQ-loaded SLN were more effective than free PZQ, leading the death of the parasites in less time. The result was proportional to doses of PZQ (25 and 50 μg mL⁻¹) and lipid concentration. Regarding cytotoxicity, the encapsulation of PZQ into SLN decreased the toxicity in HepG2 cells in comparison to the free PZQ. From the obtained results, PZQ-loaded SLN could be a new drug delivery system for the schistosomiasis treatment especially in marginalized communities, improving the therapeutic efficacy and reducing the toxic effects of PZQ.

Journal of Thermal Analysis and Calorimetry, 2019

The aim of this work was to study the physicochemical characterization of lipid matrices composed... more The aim of this work was to study the physicochemical characterization of lipid matrices composed of cetyl palmitate (CP)/perillyl alcohol (PA) and develop preliminary studies of SLN for potential application in cancer treatment. The pre-formulation was submitted to recrystallization process (heating–cooling) in a concentration of CP/AP (F1 1:0; F2 1:9; F3 1:3; and F4 1:1). The characterization showed TG presents a single stage of thermal decomposition for AP, F1, F2 and F3 and two stages for F4. DSC decrease of the onset temperature of CP before and after thermal decomposition in all samples indicates a reduction in crystallinity, and WAXD data confirmed DSC results, showing a reduction in the intensity of main diffraction peaks of the lipid mixtures and a presence of the amorphous portion in 2 θ angles of 21.51° and 23.62°. Results showed the binary mixtures presented a amorphous portion facilitates AP incorporation in SLN satisfactory size, polydispersity index, zeta potential and morphology was obtained.

Project funding by Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP, Sao Paulo, Brazi... more Project funding by Fundacao de Amparo a Pesquisa do Estado de Sao Paulo (FAPESP, Sao Paulo, Brazil) (FAPESP Refs. No. 2012/21219-5, 2012/15651-4, 2013/03181-6, 2013/19300-4, and 2014/21122-0), is hereby gratefully acknowledged. This work received support from CNPq, National Council for Scientific and Technological Development – Brazil, in the form of Research Productivity (PQ) fellowships granted to Victor M. Balcao and Marco V. Chaud.

Nanomaterials

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Journal of Biomedical Materials Research Part B, Jul 7, 2015

Drug Delivery and Translational Research

The aim of this work was to develop a dense lamellar scaffold, as a biomimetic material with pote... more The aim of this work was to develop a dense lamellar scaffold, as a biomimetic material with potential applications in the regeneration of tracheal tissue after surgical tumor resection. The scaffolds were produced by plastic compression technique, exploiting the use of total phenolic compounds (TPC) from Psidium guajava Linn as a potential cross-linking agent in a polymeric mixture based on collagen (COL), silk fibroin (SF), and polyethylene glycol 400 (PEG 400). Fourier transform infrared spectroscopy (FTIR) and differential scanning calorimetry (DSC) confirmed the chemical interactions between the polymers and the cross-linking of TPC between COL and SF. Morphological analyses showed scaffolds with porosity, interconnectivity, and a porous surface structure with a gyroid-like geometry. The analysis of the anisotropic degree resulted in anisotropic structures (0.1% TFC and 0.3% TFC) and an isotropic structure (0.5% TFC). In the mechanical properties, it was evidenced greater resis...

SDRP Journal of Biomedical Engineering, 2017

RPS Pharmacy and Pharmacology Reports

Objectives Nanotechnology-based nasal delivery systems have gained interest as a way of overcomin... more Objectives Nanotechnology-based nasal delivery systems have gained interest as a way of overcoming low drug bioavailability, limited brain exposure, fast metabolism and elimination, high doses and unwanted side effects. The main benefits of intranasal (IN) administration include the non-invasive method, easy accessibility, porous epithelial barrier and highly vascularized tissue. However, it is imperative to identify interactions between the materials used and the nasal biological environment to ensure proper release. Key findings We have examined principal factors in nasal administration, such as physiological aspects of the nose, drug and biopharmaceutical properties, formulation properties and nasal devices for drug delivery. Summary This review highlights new insights into different nanotechnology-based approaches for IN use and focuses on recent developments in topical nasal DDS, outlining the advantages and limitations of each system. This article confines itself to research a...

Biointerface Research in Applied Chemistry, 2020

The aim of this study was to investigate the influence of the extraction method on the dry extrac... more The aim of this study was to investigate the influence of the extraction method on the dry extract yield of guava leaves, correlating the total phenolic content (TPC) with the antioxidant activity. The dry extracts were obtained from hydroethanolic (50 and 70%) extract using the ultrasound-assisted method. Folin-Ciocalteau reagent was used to determine the content of TPC. DPPH (2,2-diphenyl-1-picrylhydrazyl) in vitro assay was used to determine the ability to scavenge free radicals. The results analyses demonstrated that the ultrasound-assisted method produced a higher yield in both dry extracts (11%), in contrast to the conventional method. The 50% hydroethanolic solvent was more efficient in the extraction of bioactive compounds. Both extracts showed a positive correlation of phenolic content with antioxidant activity. The FTIR spectrograms showed changes in the chemical groups, as well as determining the aromaticity index of the extracts, indicating a higher aromatic prevalence t...

Archives of Pharmacy and Pharmaceutical Sciences, 2020

Molecules, 2019

Solid lipid nanoparticles (SLNs) can be produced by various methods, but most of them are difficu... more Solid lipid nanoparticles (SLNs) can be produced by various methods, but most of them are difficult to scale up. Supercritical fluid (SCF) is an important tool to produce micro/nanoparticles with a narrow size distribution and high encapsulation efficiency. The aim of this work was to produce cetyl palmitate SLNs using SCF to be loaded with praziquantel (PZQ) as an insoluble model drug. The mean particle size (nm), polydispersity index (PdI), zeta potential, and encapsulation efficiency (EE) were determined on the freshly prepared samples, which were also subject of Differential Scanning Calorimetry (DSC), Fourier-Transform Infrared Spectroscopy (FTIR), drug release profile, and in vitro cytotoxicity analyses. PZQ-SLN exhibited a mean size of ~25 nm, PdI ~ 0.5, zeta potential ~−28 mV, and EE 88.37%. The DSC analysis demonstrated that SCF reduced the crystallinity of cetyl palmitate and favored the loading of PZQ into the lipid matrices. No chemical interaction between the PZQ and ce...

Toxics

Multi-Walled Carbon Nanotubes (MWCNT) have been functionalized with rutin through three steps (i.... more Multi-Walled Carbon Nanotubes (MWCNT) have been functionalized with rutin through three steps (i. reaction step; ii. purification step; iii. drying step) and their physicochemical properties investigated with respect to morphological structure, thermal analysis, Fourier Transform Infrared Spectroscopy (FTIR), and cytotoxicity. The molecular docking suggested the rutin-functionalized MWCNT occurred by hydrogen bonds, which was confirmed by FTIR assays, corroborating the results obtained by thermal analyses. A tubular shape, arranged in a three-dimensional structure, could be observed. Mild cytotoxicity observed in 3T3 fibroblasts suggested a dose–effect relationship after exposure. These findings suggest the formation of aggregates of filamentous structures on the cells favoring the cell penetration.