J. Lasfargues | Université Paris Descartes (original) (raw)
Papers by J. Lasfargues
Clinical oral investigations, 2003
The objective of the present study was to assess the efficiency and benefit of a chemomechanical ... more The objective of the present study was to assess the efficiency and benefit of a chemomechanical system for carious dentin removal, Carisolv, in general practice. A revised caries classification, the site/stage concept, was used to describe the clinical situations of all carious lesions treated. The study was performed by 12 investigators, and 120 carious lesions were treated with Carisolv. Sixty percent of the cases were treated without anaesthesia, and we found a significant correlation between chemomechanical treatment without anaesthesia and absence of pain ( P=0.01). In 78.3% of the cases, carious dentin was totally removed with Carisolv, and in 21.7%, the dentin treatment was completed by drilling. In cases performed with Carisolv alone, the time required to remove carious dentin was 11.1+/-9.51 min (mean+/-SD). Treatment time was equivalent for all sites and increased significantly with each successive stage of lesion progression ( P<0.001). In 82.5% of cases, the clinicia...
Clinical Oral Investigations, 2000
Bone sialoprotein (BSP), an osteogenic protein (OP), mixed with a carrier, was implanted in the p... more Bone sialoprotein (BSP), an osteogenic protein (OP), mixed with a carrier, was implanted in the pulp of rat first upper molars (OP group). Cavities were prepared with dental burs and pulp perforation was carried out by pressure with the tip of a steel probe. After 8, 14, and 30 days, the rats were killed and the pulps of the OP group were compared with (1) a sham group (S group), (2) a group where the carrier was implanted alone (C group), and (3) capping with calcium hydroxide (Ca group). After 8 days, a few inflammatory cells were seen, mostly located at the pulp surface near the perforation. In the Ca group, a dentin bridge started to form, in contrast to the other groups. After 15 days, globular structures were seen in the pulps of the S and C groups. A reparative osteodentin bridge isolated the pulp from the cavity in the Ca group. Variable reactions were seen in the OP group, with some evidence of cell and matrix alignments or plugs of osteodentin in continuity with an inner layer of reparative dentin. After 30 days, irregular osteodentin formation was observed in the pulps of the S and C groups, with a tendency for globular structures to merge, but with interglobular spaces filled by pulp remnants. In the Ca group, osteodentin was observed in the mesial part of the pulp chamber. In the BSP-implanted group, the osteogenic protein stimulated the formation of a homogeneous dentin-like deposit occupying most of the mesial part of the pulp. Apparently, BSP stimulates the differentiation of cells which secrete an organized extracellular matrix more efficiently than any other capping material used so far. Altogether, the results reported here support that bone sialoprotein displays novel bioactive properties and is capable of stimulating in 1 month's time the development of a thick reparative dentinal tissue in the pulp, occluding the perforation and filling the mesial third of the pulp chamber.
Clinical Oral Investigations, 2010
It has been reported that patients undergoing orthodontic treatment present a high risk of caries... more It has been reported that patients undergoing orthodontic treatment present a high risk of caries. Recently, an immediate chair-side test was proposed, displaying the intra-oral lactic acid production of cariogenic bacteria. The aim of this 12-month follow-up prospective cohort study was to evaluate the association between having a high score on this test and caries occurrence in 110 young patients scheduled for orthodontic treatment. Caries occurrence was studied by Kaplan-Meier curves and Multivariate Cox models allowed the examination of its association with covariates. Fifty four patients developed at least one carious lesion during the follow-up period. At baseline, approximately 70% of the patients presented a high risk of caries according to the test and this number came close to 80% by the study's completion. According to the Kaplan-Meier estimator, 51% (CI(95%) 0.40, 0.60) of the sample would have developed at least one carious lesion during the follow-up. The test score was then associated with age, DMFT, and caries occurrence. This study showed that a high test score at baseline associated with a high DMFT predicted a high risk of caries (RR = 2.6). Taking the patient's age into consideration, an increase of 1 year resulted in a 10% decrease of the risk of caries occurrence (RR = 0.89). Within the limits of this longitudinal study, it may be concluded that this test is useful to evaluate the risk for dental caries in adolescents with orthodontic treatment. Furthermore, the distribution of the lesions in our sample suggests specific clinical approaches for this group of patients.
BDJ, 2014
The concept of minimal intervention in oral medicine is based on advances in biological sciences ... more The concept of minimal intervention in oral medicine is based on advances in biological sciences applied to the dental organ. Many cultural barriers, economic as well as technical, have thwarted the application of micro-invasive conservative techniques by the general practitioner. Emerging technologies do not remove all obstacles but promote the integration of less invasive techniques in daily practice. Sono-abrasion is a technique for the selective preparation of enamel and dentine offering excellent efficacy, quality and safety. The authors describe the therapeutic principles, the choice of instrumentation and its mode of action and discuss its interest in adhesive restorative dentistry. The illustrated clinical situations focus on the preservation and optimisation of tissue bonding for both initial lesions and advanced lesions.
Advances in Dental Research, 2001
To evaluate the effects of bioactive molecules in pulpal wound healing, we carried out experiment... more To evaluate the effects of bioactive molecules in pulpal wound healing, we carried out experiments using the rat upper molars as an in vivo model. Cavities were prepared on the mesial aspect, and pulp perforation was accomplished by the application of pressure with the tip of a steel probe. After the pulp-capping procedure, the cavities were filled with a glass-ionomer cement. Comparison was made between and among: (1) sham-operated controls with dentin and predentin fragments implanted in the pulp during perforation after 8, 14, and 28 days; (2) carrier without bioactive substance; (3) calcium hydroxide; (4) Bone Sialoprotein (BSP); (5) different concentrations of Bone Morphogenetic Protein-7 (BMP-7), also termed Osteogenic Protein-1 (OP-1); and (6) N-Acetyl Cysteine (NAC), an anti-oxidant agent preventing glutathione depletion. Histologic and morphometric comparison, carried out among the first 4 groups on demineralized tissue sections, indicated that, at 28 days after implantation, BSP was the most efficient bioactive molecule, inducing homogeneous and wellmineralized reparative dentin. BMP-7 gave reparative dentin of the osteodentin type in the coronal part of the pulp, and generated the formation of a homogeneous mineralized structure in the root canal. These findings indicate that the crown and radicular parts of the pulp bear their own specificity. Both BSP and BMP-7 were superior to calcium hydroxide in their mineralization-inducing properties, and displayed larger areas of mineralization containing fewer pulp tissue inclusions. The overall mineralization process to these molecules appeared to proceed by mechanisms that involved the recruitment of cells which differentiate into osteoblast-like cells, producing a mineralizing extracellular matrix. We also provide preliminary evidence that NAC induces reparative dentin formation in the rat molar model. Pulp-capping with bioactive molecules provides new prospects for dental therapy.
![Research paper thumbnail of [Mineralization of the dental pulp: contributions of tissue engineering to tomorrow's therapeutics in odontology]](https://attachments.academia-assets.com/43027602/thumbnails/1.jpg)
](Pathologie-biologie, 2002
When bioactive molecules such as bone sialoprotein (BSP), bone morphogenetic protein-7 (BMP-7, al... more When bioactive molecules such as bone sialoprotein (BSP), bone morphogenetic protein-7 (BMP-7, also termed OP-1) and chondrogenic Inducing Agents (CIA, A+4 and A-4) were implanted in the pulp of the first upper molars, mineralizations were induced. They were either limited to the formation of a reparative dentinal bridge closing the pulpal wound (CIA A+4), or filled the mesial part of the coronal pulp (BSP), or filled totally the pulp located in the root canal (BMP-7 and CIA A-4). Consequently, these molecules may change in the next future the every day practice in dentistry.
Clinical oral investigations, 2003
The objective of the present study was to assess the efficiency and benefit of a chemomechanical ... more The objective of the present study was to assess the efficiency and benefit of a chemomechanical system for carious dentin removal, Carisolv, in general practice. A revised caries classification, the site/stage concept, was used to describe the clinical situations of all carious lesions treated. The study was performed by 12 investigators, and 120 carious lesions were treated with Carisolv. Sixty percent of the cases were treated without anaesthesia, and we found a significant correlation between chemomechanical treatment without anaesthesia and absence of pain ( P=0.01). In 78.3% of the cases, carious dentin was totally removed with Carisolv, and in 21.7%, the dentin treatment was completed by drilling. In cases performed with Carisolv alone, the time required to remove carious dentin was 11.1+/-9.51 min (mean+/-SD). Treatment time was equivalent for all sites and increased significantly with each successive stage of lesion progression ( P<0.001). In 82.5% of cases, the clinicia...
Clinical Oral Investigations, 2000
Bone sialoprotein (BSP), an osteogenic protein (OP), mixed with a carrier, was implanted in the p... more Bone sialoprotein (BSP), an osteogenic protein (OP), mixed with a carrier, was implanted in the pulp of rat first upper molars (OP group). Cavities were prepared with dental burs and pulp perforation was carried out by pressure with the tip of a steel probe. After 8, 14, and 30 days, the rats were killed and the pulps of the OP group were compared with (1) a sham group (S group), (2) a group where the carrier was implanted alone (C group), and (3) capping with calcium hydroxide (Ca group). After 8 days, a few inflammatory cells were seen, mostly located at the pulp surface near the perforation. In the Ca group, a dentin bridge started to form, in contrast to the other groups. After 15 days, globular structures were seen in the pulps of the S and C groups. A reparative osteodentin bridge isolated the pulp from the cavity in the Ca group. Variable reactions were seen in the OP group, with some evidence of cell and matrix alignments or plugs of osteodentin in continuity with an inner layer of reparative dentin. After 30 days, irregular osteodentin formation was observed in the pulps of the S and C groups, with a tendency for globular structures to merge, but with interglobular spaces filled by pulp remnants. In the Ca group, osteodentin was observed in the mesial part of the pulp chamber. In the BSP-implanted group, the osteogenic protein stimulated the formation of a homogeneous dentin-like deposit occupying most of the mesial part of the pulp. Apparently, BSP stimulates the differentiation of cells which secrete an organized extracellular matrix more efficiently than any other capping material used so far. Altogether, the results reported here support that bone sialoprotein displays novel bioactive properties and is capable of stimulating in 1 month's time the development of a thick reparative dentinal tissue in the pulp, occluding the perforation and filling the mesial third of the pulp chamber.
Clinical Oral Investigations, 2010
It has been reported that patients undergoing orthodontic treatment present a high risk of caries... more It has been reported that patients undergoing orthodontic treatment present a high risk of caries. Recently, an immediate chair-side test was proposed, displaying the intra-oral lactic acid production of cariogenic bacteria. The aim of this 12-month follow-up prospective cohort study was to evaluate the association between having a high score on this test and caries occurrence in 110 young patients scheduled for orthodontic treatment. Caries occurrence was studied by Kaplan-Meier curves and Multivariate Cox models allowed the examination of its association with covariates. Fifty four patients developed at least one carious lesion during the follow-up period. At baseline, approximately 70% of the patients presented a high risk of caries according to the test and this number came close to 80% by the study's completion. According to the Kaplan-Meier estimator, 51% (CI(95%) 0.40, 0.60) of the sample would have developed at least one carious lesion during the follow-up. The test score was then associated with age, DMFT, and caries occurrence. This study showed that a high test score at baseline associated with a high DMFT predicted a high risk of caries (RR = 2.6). Taking the patient's age into consideration, an increase of 1 year resulted in a 10% decrease of the risk of caries occurrence (RR = 0.89). Within the limits of this longitudinal study, it may be concluded that this test is useful to evaluate the risk for dental caries in adolescents with orthodontic treatment. Furthermore, the distribution of the lesions in our sample suggests specific clinical approaches for this group of patients.
BDJ, 2014
The concept of minimal intervention in oral medicine is based on advances in biological sciences ... more The concept of minimal intervention in oral medicine is based on advances in biological sciences applied to the dental organ. Many cultural barriers, economic as well as technical, have thwarted the application of micro-invasive conservative techniques by the general practitioner. Emerging technologies do not remove all obstacles but promote the integration of less invasive techniques in daily practice. Sono-abrasion is a technique for the selective preparation of enamel and dentine offering excellent efficacy, quality and safety. The authors describe the therapeutic principles, the choice of instrumentation and its mode of action and discuss its interest in adhesive restorative dentistry. The illustrated clinical situations focus on the preservation and optimisation of tissue bonding for both initial lesions and advanced lesions.
Advances in Dental Research, 2001
To evaluate the effects of bioactive molecules in pulpal wound healing, we carried out experiment... more To evaluate the effects of bioactive molecules in pulpal wound healing, we carried out experiments using the rat upper molars as an in vivo model. Cavities were prepared on the mesial aspect, and pulp perforation was accomplished by the application of pressure with the tip of a steel probe. After the pulp-capping procedure, the cavities were filled with a glass-ionomer cement. Comparison was made between and among: (1) sham-operated controls with dentin and predentin fragments implanted in the pulp during perforation after 8, 14, and 28 days; (2) carrier without bioactive substance; (3) calcium hydroxide; (4) Bone Sialoprotein (BSP); (5) different concentrations of Bone Morphogenetic Protein-7 (BMP-7), also termed Osteogenic Protein-1 (OP-1); and (6) N-Acetyl Cysteine (NAC), an anti-oxidant agent preventing glutathione depletion. Histologic and morphometric comparison, carried out among the first 4 groups on demineralized tissue sections, indicated that, at 28 days after implantation, BSP was the most efficient bioactive molecule, inducing homogeneous and wellmineralized reparative dentin. BMP-7 gave reparative dentin of the osteodentin type in the coronal part of the pulp, and generated the formation of a homogeneous mineralized structure in the root canal. These findings indicate that the crown and radicular parts of the pulp bear their own specificity. Both BSP and BMP-7 were superior to calcium hydroxide in their mineralization-inducing properties, and displayed larger areas of mineralization containing fewer pulp tissue inclusions. The overall mineralization process to these molecules appeared to proceed by mechanisms that involved the recruitment of cells which differentiate into osteoblast-like cells, producing a mineralizing extracellular matrix. We also provide preliminary evidence that NAC induces reparative dentin formation in the rat molar model. Pulp-capping with bioactive molecules provides new prospects for dental therapy.
Pathologie-biologie, 2002
When bioactive molecules such as bone sialoprotein (BSP), bone morphogenetic protein-7 (BMP-7, al... more When bioactive molecules such as bone sialoprotein (BSP), bone morphogenetic protein-7 (BMP-7, also termed OP-1) and chondrogenic Inducing Agents (CIA, A+4 and A-4) were implanted in the pulp of the first upper molars, mineralizations were induced. They were either limited to the formation of a reparative dentinal bridge closing the pulpal wound (CIA A+4), or filled the mesial part of the coronal pulp (BSP), or filled totally the pulp located in the root canal (BMP-7 and CIA A-4). Consequently, these molecules may change in the next future the every day practice in dentistry.