Dodi Darmakusuma | Universitas Nusa Cendana (original) (raw)
Papers by Dodi Darmakusuma
International journal of innovative science and research technology, Mar 14, 2024
Moringa Oleifera (Moringa oleifera Lamk) found excessive growth in dry East Nusa Tenggara (NTT) p... more Moringa Oleifera (Moringa oleifera Lamk) found excessive growth in dry East Nusa Tenggara (NTT) province. The plant has not only been primarily consumed as food especially for its leaves but also is rich in nutrients in its fruits to roots, benefits as coagulants, vitamins, and medicines. This research studied the toxic effect of the water extract of Moringa leaves to Artemia Salina Leach shrimp larvae according to BST method using water (room temperature) as a solvent, a preliminary study of the antibacterial and the anticancer target of study of the Morinaga. Moringa leaves were macerated for 3 x 24 hours using water, the results of phytochemical tests identified secondary metabolic content, among others, alkaloids, flavonoids, triterpenes, and tannins. The results of the BSLT test showed that the water extract (room temperature) of Moringa leaves had a toxicity level against Artemia Salina Leach as indicated by an LC50 value of 888, 34 less than 1000 ppm. It can be concluded that Moringa leaves have potential as a plant which can later be used as anti-bacterial and anti-cancer.
![Research paper thumbnail of Aplikasi Asap Cair Tempurung Kelapa Rendah Benzo[a] Pyrene untuk Meningkatkan Kualitas Se’i Sapi Bali](https://attachments.academia-assets.com/108061127/thumbnails/1.jpg)
](Sains Peternakan, Mar 28, 2017
Penelitian ini telah dilakukan di Kecamatan Amarasi Kabupaten Kupang untuk mendapatkan asap cair ... more Penelitian ini telah dilakukan di Kecamatan Amarasi Kabupaten Kupang untuk mendapatkan asap cair tempurung kelapa, Laboratorium Institut Pertanian Bogor menguji kandungan benzo[a]pirene dan total bakteri pada daging, Laboratorium riset terpadu Undana melakukan destilasi asap cair dan mendapatkan nilai organoleptik dan Laboratorium Politeknik Pertanian Kupang menguji kualitas kimia daging se'i sapi Bali. Penelitian ini di lakukan selama 2 bulan yaitu tanggal 26 April sampai 26 Juni 2016. Penelitian ini bertujuan untuk mengetahui kandungan benzo[a]pirene terendah dalam asap cair tempurung kelapa dengan melakukan beberapa proses destilasi untuk diaplikasikan ke daging se'i sapi Bali. Materi yang digunakan adalah tempurung kelapa sebanyak 20 kg, daging sapi segar sebanyak 5 kg yang diambil pada bagian otot. Rancangan dasar yang digunakan adalah, rancangan acak lengkap (RAL) dengan 6 perlakuan dan 3 kali ulangan sehingga diperoleh 18 unit percobaan. Analisis data dilakukan dengan analisis non parametrik tes Kruskall Wallis dan analisis dengan metode Analisis of Variance (ANOVA). Apabila terdapat perbedaan antar perlakuan maka dilanjutkan dengan uji Beda Nyata Terkecil (BNT). Hasil penelitian menunjukan: total bakteri yang terdapat dalam daging se'i tidak berbeda nyata P≤0,05 dari perlakuan yang menggunakan asap cair terhadap daging se'i tradisional P0. 6,6x106 P1. 6,2x106 P2. 5,4x106 P3. 1,7x106 P4. 4,46x103 P5. 3,0x103 sedangkan pada kualitas kimia daging se'i sapi Bali berpengaruh sangat nyata P≤0,01 dari setiap perlakuan. simpulan pada penelitian adalah: semakin banyak presentase asap cair maka jumlah bakteri pada se'i sapi semakin berkurang dan rataan kualitas kimia daging se'i sapi Bali akan semakin meningkat.
Research on toxicity test of Sarcophyton sp. extract from Timor against Artemia s. Leach Nauplii ... more Research on toxicity test of Sarcophyton sp. extract from Timor against Artemia s. Leach Nauplii has been conducted. Sarcophyton sp. from Paradiso beach, Kupang, was macerated in n-hexane, ethyl acetate, and methanol then each macerate was evaporated at 40 0 C and 45 0 C to dry. The dried extract resulted was tested its secondary metabolite content and toxicity using Brine Shrimp Lethality Test (BSLT). The results showed that the methanol extract contains saponins, phenols, and steroids while the ethyl acetate extract contains steroids and terpenoids. The toxicity test against artemia showed that the methanol extract from Sarcophyton sp. is very toxic to the nauplii with LC 50 value of 29,56 ppm while the ethyl acetate extract is classified toxic and gave LC 50 value of 34,11 ppm.
Jurnal Sain Peternakan Indonesia, Dec 26, 2021
The leading cause of malnutrition in NTT province is the lack of balanced nutrition, one of which... more The leading cause of malnutrition in NTT province is the lack of balanced nutrition, one of which is macronutrients such as carbohydrates, proteins, and fats. So there is a need for innovation from livestock products rich in readily available animal protein, one of which is chicken. Utilization of the results of heating technology Moringa leaf flour which is rich in micro and macronutrients is available in the province of NTT, so that it can provide processed products in the form of chicken sausage chips with the addition of Moringa leaf flour. This study aims to determine the effect of adding Moringa leaf flour P0, P1, P2, and P3 on chicken sausage chips' chemical characteristics and nutritional value. Each treatment P0 Moringa leaf flour (0%), P1 Moringa leaf flour (1%), P2 Moringa leaf flour (2%), P3 Moringa leaf flour (3%). The experimental method used is a simple, completely randomized design (CRD) with four treatments and four replications. Analysis of the ANOVA (Analysis of Variance) data and Duncan's further test, while the nutritional value content was calculated according to BPOM regulation NO 19 of 2019 concerning Nutrition Label Reference. The results showed that the addition of Moringa leaf flour had a significant effect on the chemical characteristics of P<0.05. According to the National Food and Drug Administration Agency (BPOM), for appropriate nutritional content, information on the nutritional value of chicken sausage chips.
Rasayan journal of Chemistry, 2021
Psidium guajava was abundantly found in arid land of Timor and traditionally used for treatment a... more Psidium guajava was abundantly found in arid land of Timor and traditionally used for treatment against diarhea. Research had been performed to analyze the chemical profiles together with antibacterial and fruit flies attractant activity of the leaf essential oil from P. guajava. The powder of dried leaves was subjected to hydro-distillation using Clevenger apparatus to obtain essential oil with a yield of 0.5%. Analysis of GC-MS spectra revealed that limone and β-caryophyllene were the main components of the essential oil. The essential oil showed strong antibacterial activity with the zone inhibition was observed at 16.3 for Staphyloccus aureus and 15.9 mm for Escherechia coli. On the other hand, no fruit flies were trapped during the field test in guava fruit.
Molecules, Nov 19, 2019
Timor Island is very hot and dry due to the high intensity of sunlight experienced throughout the... more Timor Island is very hot and dry due to the high intensity of sunlight experienced throughout the year. The endophytic fungi Aspergillus flavus had been isolated from medicinal plants such as Catharanthus roseus, Annona squamosa and Curcuma xanthorisa. The endophytic fungi A. flavus from each plant was cultivated on solid rice media and then analyzed for its capability for producing kojic acid. The production of kojic acid was analyzed by HPLC; the highest amount of kojic acid was observed from the endophytic fungi A. flavus, isolated from the stem of Catharanthus roseus, followed by A. flavus from Annona squamosa and Curcuma xanthorisa. Simple VLC fractionation of the extract of A. flavus from C.roseus led to the isolation of around 11.1 g of pure kojic acid. The structure of kojic acid (1) was confirmed by NMR and MS spectroscopic data. A comparison of the NMR data with the literature supported the revision of the natural product flufuran to kojic acid. To the best of our knowledge, this is the first report of a strain of endophytic fungi producing only kojic acid without any other toxic metabolites such as alfatoxins. Therefore, this Aspergillus flavus strain can be applied as a potential producer of kojic acid for industrial use.
International Journal Of Scientific Advances
The study has purpose for knowing the effect of the addition of broccoli on antioxidant activity,... more The study has purpose for knowing the effect of the addition of broccoli on antioxidant activity, and the chemical quality of beef meatballs. The experimental design used was a completely randomized design (CRD) consisting of 5 treatments and 4 replications. The treatment was meatballs without adding broccoli as a control (T0), adding broccoli with levels of 10% (T1), 12% (T2), 14% (T3) and 16% (T4) of the weight of the meat. The results showed that broccoli increases antioxidant activity because it contains natural antioxidants, increases fiber content because it contains high fiber and reduces fat content because it contains beta carotene. Analysis of variance showed that broccoli content at 16% by weight of meat had a significant effect (P<0.05) on antioxidant activity and fiber content. Broccoli content of 10% by weight of meat had a significant effect (P<0.05) on fat content. From the results of this study, it can be concluded that meatballs with the addition of broccoli ...
Jurnal Berkala Ilmiah Sains dan Terapan Kimia
The influence of rice types on the purification of rice husk ash was investigated by extracting s... more The influence of rice types on the purification of rice husk ash was investigated by extracting silica and purifying it with a 12% NaOH solution, followed by the coprecipitation technique. Rice husk samples were from Mbay (Nagekeo Regency) with two different varieties, Ciherang and Inpari 42, and from Soa (Ngada Regency) with Ciherang variety. The silica produced in this research was characterized using UV Vis spectrophotometry to obtain the surface area value. The surface acidity value was calculated using the acidbase titration method. Using XRF, the purity of silica was determined. Ciherang Mbay, Ciherang Soa, and Inpari Mbay had surface area values of 18.397 m2/gram, 18.347 m 2 /gram, and 18.491 m 2 /gram, respectively. The surface acidity value of the three samples was 45.1 mmol/gram, 45.5 mmol/gram, and 44.7 mmol/gram, respectively. The purity of silica from rice husk ash Ciherang Mbay, Ciherang Soa, and Inpari Mbay samples based on XRF was 98.6%, 98.3%, dan 99.2%, respectively. Silica with the highest purity (Inpari Mbay variety) was applied as an adsorbent in the adsorption process of manganese (Mn(II)) metal ion with an adsorption capacity of 212.76 µmol/gram and adsorption energy of 38.165 kJ/mol through chemical adsorption.
International journal of scientific advances, 2023
The study has purpose for knowing the effect of the addition of broccoli on antioxidant activity,... more The study has purpose for knowing the effect of the addition of broccoli on antioxidant activity, and the chemical quality of beef meatballs. The experimental design used was a completely randomized design (CRD) consisting of 5 treatments and 4 replications. The treatment was meatballs without adding broccoli as a control (T0), adding broccoli with levels of 10% (T1), 12% (T2), 14% (T3) and 16% (T4) of the weight of the meat. The results showed that broccoli increases antioxidant activity because it contains natural antioxidants, increases fiber content because it contains high fiber and reduces fat content because it contains beta carotene. Analysis of variance showed that broccoli content at 16% by weight of meat had a significant effect (P<0.05) on antioxidant activity and fiber content. Broccoli content of 10% by weight of meat had a significant effect (P<0.05) on fat content. From the results of this study it can be concluded that meatballs with the addition of broccoli with a content of 16% by weight of meat, produce beef meatballs with good antioxidant activity and chemical quality.
Zenodo (CERN European Organization for Nuclear Research), Dec 22, 2022
Sandalwood (Santalum album L.) cultivation is an effort to use this plant in a sustainable manner... more Sandalwood (Santalum album L.) cultivation is an effort to use this plant in a sustainable manner. This cultivation effort faces time challenges considering that the harvesting period of this plant takes a very long time. Therefore, this cultivation effort must be strengthened by utilizing the value of this plant before the harvest. The use of sandalwood fruit can be used as an opportunity to increase the potential economic benefits of the plant before its harvest. Communities in parts of East Nusa Tenggara have traditionally used sandalwood to increase their immune systems. The use of sandalwood fruit as an antioxidant functional food commodity is an urgent need, based on the description of this plant's traditional use of its fruit. The use of a simple extraction method with a decoction method using hot water needs to be considered to make it easier for the community to produce sandalwood fruit extract. The purpose of this study was to evaluate the antioxidant activity and total phenol content of sandalwood fruit hot water extract. The IC50 value of the antioxidant activity of sandalwood hot water extract is 154.07 ppm. The total amount of phenols contained in the extract is directly related to this antioxidant activity. The total phenol content of the hot water extract of sandalwood fruit is 76,012 ± 1,653 mg GAE/g. The results of this study show that sandalwood ethanol extract has higher antioxidant activity than sandalwood hot water extract (IC50 = 108.98ppm). Total phenol calculation results support this claim. Compared to the hot water extract, the ethanol extract of sandalwood fruit contains more total phenols. Nevertheless, the antioxidant activity of sandalwood fruit water extract is still sufficient.
THE 9TH INTERNATIONAL CONFERENCE OF THE INDONESIAN CHEMICAL SOCIETY ICICS 2021: Toward a Meaningful Society
This research was conducted to determine the effect of volume variations of sodium silicate solut... more This research was conducted to determine the effect of volume variations of sodium silicate solution (10, 30, and 50 mL) on the surface acidity and surface area of silica produced by rice husk ash processing, as well as the effect of mass variations of chitosan (0.3; 0.5 and 0.7 grams) on the antibacterial plastic properties of silica immobilized with EDTA-Cu and chitosan. The surface acidity test using the titration method showed that the most significant surface acidity was obtained in the volume of 10 mL sodium silicate solution (1.4 mmol/g). In addition, the methylene blue method shows that the surface area with a volume of 10 mL sodium silicate solution (185.3203 m 2 /g) is slightly larger than those of the other volume variations. The characterization results using FTIR showed the presence of siloxane (≡Si-O-Si≡) and silanol (≡Si-OH) groups in EDTA-Cu immobilized silica, which was similar to silica gel. Based on this study, it is known that there is an effect of mass variations of chitosan on the synthesized plastic. For instance, the percent elongation with the chitosan mass of 0.3 grams (22%) is higher than those with 0.5 grams (10%) and 0.7 grams (7.5%). The condensation test also gave the lowest percent condensation at 0.3 grams of chitosan mass. Meanwhile, the degradation test of the synthesized plastic showed varying results between 16.1 to 100%. The antibacterial properties of synthetic plastics based on the inhibitory test were strong for both Staphylococcus aureus and Escherichia coli bacteria and increased with the increasing chitosan mass.
Indonesian Journal of Chemistry
Shifting the drug discovery from plants to microorganisms due to minimizing the ecological effect... more Shifting the drug discovery from plants to microorganisms due to minimizing the ecological effects has led to the increasing work on finding bioactive metabolites from endophytic fungi, especially those isolated from medicinal plants growing in arid and semi-arid land. An endophytic fungi Alternaria sp. isolated from dry land was cultivated on solid rice media and then extracted with ethyl acetate. From the fungal ethyl acetate crude extract of Alternaria sp., compounds altertoxin I (1) and II (2), and stemphyltoxin III (3), were isolated based on the bioactivity assay. The structures of compounds 1-3 were identified using UV, NMR, and mass spectral data and compared to those spectral data reported in the literature. The absolute configuration was determined to be the same as the previous report based on the comparison with the optical rotation value. The three compounds showed strong cytotoxic activity, with altertoxin II being the most active ones having the inhibition of growth o...
Chemical analysis, including metal ions determination using the spectrophotometry method, involve... more Chemical analysis, including metal ions determination using the spectrophotometry method, involves using a complexing agent, mostly produced by chemical industries, reacting with the analyte to form a colored compound. The very high cost and the environmentally unfriendly are the main disadvantages of that complexing agent. The use of a complexing agent categorized as a “green” chemical is critical to overcoming those shortcomings. In this work, extract ethanol of Terminalia catappa L. leaves was employed as a complexing agent in the determination of Fe(III) from aqueous media using the spectrophotometry method. The value of correlation coefficient (r) 0.993, obtained from the calibration curve, indicated that ethanol extract of Terminalia catappa L. leaves could be applied as a complexing agent for determining Fe(III) from aqueous media using the spectrophotometry method. By comparing this complexing agent with the commonly used complexing agent of Fe(III)...
SAINSTEK, Dec 13, 2019
Telah dilakukan penelitian aktivitas antioksidan dan aktivitas antikanker ekstrak P-100 dari buah... more Telah dilakukan penelitian aktivitas antioksidan dan aktivitas antikanker ekstrak P-100 dari buah Eucalyptus alba. Ekstrak P-100 adalah salah satu varian ekstrak air buah Eucalyptus alba yang dibuat pada suhu tinggi. Penelitian ini dilakukan untuk mengetahui potensi peredaman radikal bebas dan penghambatan proliferasi sel lestrai MCF-7 dari ekstrak P-100. Ekstrak P-100 dibuat dari buah Eucalyptus alba yang diekstraksi dengan pelarut aquadest pada temperatur 100 C dan dikeringkan pada temperatur 80 C hingga diperoleh ekstrak kering. Total kapasitas antioksidan ekstrak ditentukan sebagai aktivitas peredaman radikal bebas 1,1-difenil-2-pikril-hidrazil (DPPH). Sebagai pembanding digunakan ekstrak etanol 70% dan ekstrak etanol 80%. Uji potensi antikanker ekstrak P-100 dilakukan dengan uji sitotoksik terhadap sel lestari tumor payudara MCF-7 sebagai sel uji dengan metode 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT). Total kandungan fenol ekstrak P-100 diukur secara spektroskopi dengan pereaksi Folin-Ciocalteu. Hasil penelitian menunjukkan bahwa ekstrak P-100 memiliki aktivitas antioksidan (IC50=18.25 ppm) yang lebih tinggi dibandingkan ekstrak etanol 70% (IC50 = 22.18 ppm) dan ekstrak etanol 80% (IC50 = 24.53 ppm). Ekstrak P-100 memiliki aktivitas menghambat proliferasi sel lestari tumor payudara MCF-7 sebesar 83.42% pada konsentrasi 50 ppm. Aktivitas antioksidan dan penghambatan proliferasi ekstrak P-100 diduga berkorelasi dengan kandungan fenol total. Fenol ekstrak P-100 (42.04 0.17 mg of GAE/g) diketahui lebih tinggi daripada ektrak etanol 70% (22.81 0.35 mg of GAE/g) dan ektrak etanol 80% (15.11 0.13 mg of GAE/g). Berdasarkan hasil penelitian ini dapat disimpulkan bahwa Ekstrak P-100 dari buah Eucalyptus alba memiliki aktivitas antioksidan dan antikanker.
Chemistry Notes, Jul 2, 2018
Research on toxicity test of Sarcophyton sp. extract from Timor against Artemia s. Leach Nauplii ... more Research on toxicity test of Sarcophyton sp. extract from Timor against Artemia s. Leach Nauplii has been conducted. Sarcophyton sp. from Paradiso beach, Kupang, was macerated in n-hexane, ethyl acetate, and methanol then each macerate was evaporated at 40 0 C and 45 0 C to dry. The dried extract resulted was tested its secondary metabolite content and toxicity using Brine Shrimp Lethality Test (BSLT). The results showed that the methanol extract contains saponins, phenols, and steroids while the ethyl acetate extract contains steroids and terpenoids. The toxicity test against artemia showed that the methanol extract from Sarcophyton sp. is very toxic to the nauplii with LC 50 value of 29,56 ppm while the ethyl acetate extract is classified toxic and gave LC 50 value of 34,11 ppm.
Rasayan Journal of Chemistry, 2021
Psidium guajava was abundantly found in arid land of Timor and traditionally used for treatment a... more Psidium guajava was abundantly found in arid land of Timor and traditionally used for treatment against diarhea. Research had been performed to analyze the chemical profiles together with antibacterial and fruit flies attractant activity of the leaf essential oil from P. guajava. The powder of dried leaves was subjected to hydro-distillation using Clevenger apparatus to obtain essential oil with a yield of 0.5%. Analysis of GC-MS spectra revealed that limone and β-caryophyllene were the main components of the essential oil. The essential oil showed strong antibacterial activity with the zone inhibition was observed at 16.3 for Staphyloccus aureus and 15.9 mm for Escherechia coli. On the other hand, no fruit flies were trapped during the field test in guava fruit.
Biologi Edukasi: Jurnal Ilmiah Pendidikan Biologi, 2021
Hospital liquid waste has the potential to pollute the environment and harm health if not process... more Hospital liquid waste has the potential to pollute the environment and harm health if not processed properly because it contains high enough organic and inorganic compounds, pathogenic microorganisms, toxic chemicals and radioactive substances. Rumah Sakit Umum Daerah (RSUD) Kefamenanu is the only hospital owned by the Government of Kabupaten Timor Tengah Utara and located in the middle of community settlements and uses wastewater treatment plants (IPAL) in the treatment of hospital liquid waste since 2016. The results of liquid waste quality examination showed that there are several parameters that have increased after processing through IPAL so that researchers use filtration method in processing liquid waste of RSUD Kefamenanu. This study aims to find out the effectiveness of liquid waste treatment of RSUD Kefamenanu through filtration process using filter media in the form of coconut coir, sand, palm oil, activated charcoal and zeolite. This research is a pseudo experiment, namely to find out the percentage of decreased levels of liquid waste parameters through the filtration process. The results showed that the levels after processing had an average value of BOD 3.86 mg/L, TSS 14 mg/L and ammonia of 0.10 mg/L. The filtration process was able to lower BOD levels by 63.62%, TSS by 93.24% and ammonia by 86.57%. Based on these results, it can be concluded that the filtration process is able to lower levels of BOD, TSS and ammonia in liquid waste of RSUD Kefamenanu.
International journal of innovative science and research technology, Mar 14, 2024
Moringa Oleifera (Moringa oleifera Lamk) found excessive growth in dry East Nusa Tenggara (NTT) p... more Moringa Oleifera (Moringa oleifera Lamk) found excessive growth in dry East Nusa Tenggara (NTT) province. The plant has not only been primarily consumed as food especially for its leaves but also is rich in nutrients in its fruits to roots, benefits as coagulants, vitamins, and medicines. This research studied the toxic effect of the water extract of Moringa leaves to Artemia Salina Leach shrimp larvae according to BST method using water (room temperature) as a solvent, a preliminary study of the antibacterial and the anticancer target of study of the Morinaga. Moringa leaves were macerated for 3 x 24 hours using water, the results of phytochemical tests identified secondary metabolic content, among others, alkaloids, flavonoids, triterpenes, and tannins. The results of the BSLT test showed that the water extract (room temperature) of Moringa leaves had a toxicity level against Artemia Salina Leach as indicated by an LC50 value of 888, 34 less than 1000 ppm. It can be concluded that Moringa leaves have potential as a plant which can later be used as anti-bacterial and anti-cancer.
Sains Peternakan, Mar 28, 2017
Penelitian ini telah dilakukan di Kecamatan Amarasi Kabupaten Kupang untuk mendapatkan asap cair ... more Penelitian ini telah dilakukan di Kecamatan Amarasi Kabupaten Kupang untuk mendapatkan asap cair tempurung kelapa, Laboratorium Institut Pertanian Bogor menguji kandungan benzo[a]pirene dan total bakteri pada daging, Laboratorium riset terpadu Undana melakukan destilasi asap cair dan mendapatkan nilai organoleptik dan Laboratorium Politeknik Pertanian Kupang menguji kualitas kimia daging se'i sapi Bali. Penelitian ini di lakukan selama 2 bulan yaitu tanggal 26 April sampai 26 Juni 2016. Penelitian ini bertujuan untuk mengetahui kandungan benzo[a]pirene terendah dalam asap cair tempurung kelapa dengan melakukan beberapa proses destilasi untuk diaplikasikan ke daging se'i sapi Bali. Materi yang digunakan adalah tempurung kelapa sebanyak 20 kg, daging sapi segar sebanyak 5 kg yang diambil pada bagian otot. Rancangan dasar yang digunakan adalah, rancangan acak lengkap (RAL) dengan 6 perlakuan dan 3 kali ulangan sehingga diperoleh 18 unit percobaan. Analisis data dilakukan dengan analisis non parametrik tes Kruskall Wallis dan analisis dengan metode Analisis of Variance (ANOVA). Apabila terdapat perbedaan antar perlakuan maka dilanjutkan dengan uji Beda Nyata Terkecil (BNT). Hasil penelitian menunjukan: total bakteri yang terdapat dalam daging se'i tidak berbeda nyata P≤0,05 dari perlakuan yang menggunakan asap cair terhadap daging se'i tradisional P0. 6,6x106 P1. 6,2x106 P2. 5,4x106 P3. 1,7x106 P4. 4,46x103 P5. 3,0x103 sedangkan pada kualitas kimia daging se'i sapi Bali berpengaruh sangat nyata P≤0,01 dari setiap perlakuan. simpulan pada penelitian adalah: semakin banyak presentase asap cair maka jumlah bakteri pada se'i sapi semakin berkurang dan rataan kualitas kimia daging se'i sapi Bali akan semakin meningkat.
Research on toxicity test of Sarcophyton sp. extract from Timor against Artemia s. Leach Nauplii ... more Research on toxicity test of Sarcophyton sp. extract from Timor against Artemia s. Leach Nauplii has been conducted. Sarcophyton sp. from Paradiso beach, Kupang, was macerated in n-hexane, ethyl acetate, and methanol then each macerate was evaporated at 40 0 C and 45 0 C to dry. The dried extract resulted was tested its secondary metabolite content and toxicity using Brine Shrimp Lethality Test (BSLT). The results showed that the methanol extract contains saponins, phenols, and steroids while the ethyl acetate extract contains steroids and terpenoids. The toxicity test against artemia showed that the methanol extract from Sarcophyton sp. is very toxic to the nauplii with LC 50 value of 29,56 ppm while the ethyl acetate extract is classified toxic and gave LC 50 value of 34,11 ppm.
Jurnal Sain Peternakan Indonesia, Dec 26, 2021
The leading cause of malnutrition in NTT province is the lack of balanced nutrition, one of which... more The leading cause of malnutrition in NTT province is the lack of balanced nutrition, one of which is macronutrients such as carbohydrates, proteins, and fats. So there is a need for innovation from livestock products rich in readily available animal protein, one of which is chicken. Utilization of the results of heating technology Moringa leaf flour which is rich in micro and macronutrients is available in the province of NTT, so that it can provide processed products in the form of chicken sausage chips with the addition of Moringa leaf flour. This study aims to determine the effect of adding Moringa leaf flour P0, P1, P2, and P3 on chicken sausage chips' chemical characteristics and nutritional value. Each treatment P0 Moringa leaf flour (0%), P1 Moringa leaf flour (1%), P2 Moringa leaf flour (2%), P3 Moringa leaf flour (3%). The experimental method used is a simple, completely randomized design (CRD) with four treatments and four replications. Analysis of the ANOVA (Analysis of Variance) data and Duncan's further test, while the nutritional value content was calculated according to BPOM regulation NO 19 of 2019 concerning Nutrition Label Reference. The results showed that the addition of Moringa leaf flour had a significant effect on the chemical characteristics of P<0.05. According to the National Food and Drug Administration Agency (BPOM), for appropriate nutritional content, information on the nutritional value of chicken sausage chips.
Rasayan journal of Chemistry, 2021
Psidium guajava was abundantly found in arid land of Timor and traditionally used for treatment a... more Psidium guajava was abundantly found in arid land of Timor and traditionally used for treatment against diarhea. Research had been performed to analyze the chemical profiles together with antibacterial and fruit flies attractant activity of the leaf essential oil from P. guajava. The powder of dried leaves was subjected to hydro-distillation using Clevenger apparatus to obtain essential oil with a yield of 0.5%. Analysis of GC-MS spectra revealed that limone and β-caryophyllene were the main components of the essential oil. The essential oil showed strong antibacterial activity with the zone inhibition was observed at 16.3 for Staphyloccus aureus and 15.9 mm for Escherechia coli. On the other hand, no fruit flies were trapped during the field test in guava fruit.
Molecules, Nov 19, 2019
Timor Island is very hot and dry due to the high intensity of sunlight experienced throughout the... more Timor Island is very hot and dry due to the high intensity of sunlight experienced throughout the year. The endophytic fungi Aspergillus flavus had been isolated from medicinal plants such as Catharanthus roseus, Annona squamosa and Curcuma xanthorisa. The endophytic fungi A. flavus from each plant was cultivated on solid rice media and then analyzed for its capability for producing kojic acid. The production of kojic acid was analyzed by HPLC; the highest amount of kojic acid was observed from the endophytic fungi A. flavus, isolated from the stem of Catharanthus roseus, followed by A. flavus from Annona squamosa and Curcuma xanthorisa. Simple VLC fractionation of the extract of A. flavus from C.roseus led to the isolation of around 11.1 g of pure kojic acid. The structure of kojic acid (1) was confirmed by NMR and MS spectroscopic data. A comparison of the NMR data with the literature supported the revision of the natural product flufuran to kojic acid. To the best of our knowledge, this is the first report of a strain of endophytic fungi producing only kojic acid without any other toxic metabolites such as alfatoxins. Therefore, this Aspergillus flavus strain can be applied as a potential producer of kojic acid for industrial use.
International Journal Of Scientific Advances
The study has purpose for knowing the effect of the addition of broccoli on antioxidant activity,... more The study has purpose for knowing the effect of the addition of broccoli on antioxidant activity, and the chemical quality of beef meatballs. The experimental design used was a completely randomized design (CRD) consisting of 5 treatments and 4 replications. The treatment was meatballs without adding broccoli as a control (T0), adding broccoli with levels of 10% (T1), 12% (T2), 14% (T3) and 16% (T4) of the weight of the meat. The results showed that broccoli increases antioxidant activity because it contains natural antioxidants, increases fiber content because it contains high fiber and reduces fat content because it contains beta carotene. Analysis of variance showed that broccoli content at 16% by weight of meat had a significant effect (P<0.05) on antioxidant activity and fiber content. Broccoli content of 10% by weight of meat had a significant effect (P<0.05) on fat content. From the results of this study, it can be concluded that meatballs with the addition of broccoli ...
Jurnal Berkala Ilmiah Sains dan Terapan Kimia
The influence of rice types on the purification of rice husk ash was investigated by extracting s... more The influence of rice types on the purification of rice husk ash was investigated by extracting silica and purifying it with a 12% NaOH solution, followed by the coprecipitation technique. Rice husk samples were from Mbay (Nagekeo Regency) with two different varieties, Ciherang and Inpari 42, and from Soa (Ngada Regency) with Ciherang variety. The silica produced in this research was characterized using UV Vis spectrophotometry to obtain the surface area value. The surface acidity value was calculated using the acidbase titration method. Using XRF, the purity of silica was determined. Ciherang Mbay, Ciherang Soa, and Inpari Mbay had surface area values of 18.397 m2/gram, 18.347 m 2 /gram, and 18.491 m 2 /gram, respectively. The surface acidity value of the three samples was 45.1 mmol/gram, 45.5 mmol/gram, and 44.7 mmol/gram, respectively. The purity of silica from rice husk ash Ciherang Mbay, Ciherang Soa, and Inpari Mbay samples based on XRF was 98.6%, 98.3%, dan 99.2%, respectively. Silica with the highest purity (Inpari Mbay variety) was applied as an adsorbent in the adsorption process of manganese (Mn(II)) metal ion with an adsorption capacity of 212.76 µmol/gram and adsorption energy of 38.165 kJ/mol through chemical adsorption.
International journal of scientific advances, 2023
The study has purpose for knowing the effect of the addition of broccoli on antioxidant activity,... more The study has purpose for knowing the effect of the addition of broccoli on antioxidant activity, and the chemical quality of beef meatballs. The experimental design used was a completely randomized design (CRD) consisting of 5 treatments and 4 replications. The treatment was meatballs without adding broccoli as a control (T0), adding broccoli with levels of 10% (T1), 12% (T2), 14% (T3) and 16% (T4) of the weight of the meat. The results showed that broccoli increases antioxidant activity because it contains natural antioxidants, increases fiber content because it contains high fiber and reduces fat content because it contains beta carotene. Analysis of variance showed that broccoli content at 16% by weight of meat had a significant effect (P<0.05) on antioxidant activity and fiber content. Broccoli content of 10% by weight of meat had a significant effect (P<0.05) on fat content. From the results of this study it can be concluded that meatballs with the addition of broccoli with a content of 16% by weight of meat, produce beef meatballs with good antioxidant activity and chemical quality.
Zenodo (CERN European Organization for Nuclear Research), Dec 22, 2022
Sandalwood (Santalum album L.) cultivation is an effort to use this plant in a sustainable manner... more Sandalwood (Santalum album L.) cultivation is an effort to use this plant in a sustainable manner. This cultivation effort faces time challenges considering that the harvesting period of this plant takes a very long time. Therefore, this cultivation effort must be strengthened by utilizing the value of this plant before the harvest. The use of sandalwood fruit can be used as an opportunity to increase the potential economic benefits of the plant before its harvest. Communities in parts of East Nusa Tenggara have traditionally used sandalwood to increase their immune systems. The use of sandalwood fruit as an antioxidant functional food commodity is an urgent need, based on the description of this plant's traditional use of its fruit. The use of a simple extraction method with a decoction method using hot water needs to be considered to make it easier for the community to produce sandalwood fruit extract. The purpose of this study was to evaluate the antioxidant activity and total phenol content of sandalwood fruit hot water extract. The IC50 value of the antioxidant activity of sandalwood hot water extract is 154.07 ppm. The total amount of phenols contained in the extract is directly related to this antioxidant activity. The total phenol content of the hot water extract of sandalwood fruit is 76,012 ± 1,653 mg GAE/g. The results of this study show that sandalwood ethanol extract has higher antioxidant activity than sandalwood hot water extract (IC50 = 108.98ppm). Total phenol calculation results support this claim. Compared to the hot water extract, the ethanol extract of sandalwood fruit contains more total phenols. Nevertheless, the antioxidant activity of sandalwood fruit water extract is still sufficient.
THE 9TH INTERNATIONAL CONFERENCE OF THE INDONESIAN CHEMICAL SOCIETY ICICS 2021: Toward a Meaningful Society
This research was conducted to determine the effect of volume variations of sodium silicate solut... more This research was conducted to determine the effect of volume variations of sodium silicate solution (10, 30, and 50 mL) on the surface acidity and surface area of silica produced by rice husk ash processing, as well as the effect of mass variations of chitosan (0.3; 0.5 and 0.7 grams) on the antibacterial plastic properties of silica immobilized with EDTA-Cu and chitosan. The surface acidity test using the titration method showed that the most significant surface acidity was obtained in the volume of 10 mL sodium silicate solution (1.4 mmol/g). In addition, the methylene blue method shows that the surface area with a volume of 10 mL sodium silicate solution (185.3203 m 2 /g) is slightly larger than those of the other volume variations. The characterization results using FTIR showed the presence of siloxane (≡Si-O-Si≡) and silanol (≡Si-OH) groups in EDTA-Cu immobilized silica, which was similar to silica gel. Based on this study, it is known that there is an effect of mass variations of chitosan on the synthesized plastic. For instance, the percent elongation with the chitosan mass of 0.3 grams (22%) is higher than those with 0.5 grams (10%) and 0.7 grams (7.5%). The condensation test also gave the lowest percent condensation at 0.3 grams of chitosan mass. Meanwhile, the degradation test of the synthesized plastic showed varying results between 16.1 to 100%. The antibacterial properties of synthetic plastics based on the inhibitory test were strong for both Staphylococcus aureus and Escherichia coli bacteria and increased with the increasing chitosan mass.
Indonesian Journal of Chemistry
Shifting the drug discovery from plants to microorganisms due to minimizing the ecological effect... more Shifting the drug discovery from plants to microorganisms due to minimizing the ecological effects has led to the increasing work on finding bioactive metabolites from endophytic fungi, especially those isolated from medicinal plants growing in arid and semi-arid land. An endophytic fungi Alternaria sp. isolated from dry land was cultivated on solid rice media and then extracted with ethyl acetate. From the fungal ethyl acetate crude extract of Alternaria sp., compounds altertoxin I (1) and II (2), and stemphyltoxin III (3), were isolated based on the bioactivity assay. The structures of compounds 1-3 were identified using UV, NMR, and mass spectral data and compared to those spectral data reported in the literature. The absolute configuration was determined to be the same as the previous report based on the comparison with the optical rotation value. The three compounds showed strong cytotoxic activity, with altertoxin II being the most active ones having the inhibition of growth o...
Chemical analysis, including metal ions determination using the spectrophotometry method, involve... more Chemical analysis, including metal ions determination using the spectrophotometry method, involves using a complexing agent, mostly produced by chemical industries, reacting with the analyte to form a colored compound. The very high cost and the environmentally unfriendly are the main disadvantages of that complexing agent. The use of a complexing agent categorized as a “green” chemical is critical to overcoming those shortcomings. In this work, extract ethanol of Terminalia catappa L. leaves was employed as a complexing agent in the determination of Fe(III) from aqueous media using the spectrophotometry method. The value of correlation coefficient (r) 0.993, obtained from the calibration curve, indicated that ethanol extract of Terminalia catappa L. leaves could be applied as a complexing agent for determining Fe(III) from aqueous media using the spectrophotometry method. By comparing this complexing agent with the commonly used complexing agent of Fe(III)...
SAINSTEK, Dec 13, 2019
Telah dilakukan penelitian aktivitas antioksidan dan aktivitas antikanker ekstrak P-100 dari buah... more Telah dilakukan penelitian aktivitas antioksidan dan aktivitas antikanker ekstrak P-100 dari buah Eucalyptus alba. Ekstrak P-100 adalah salah satu varian ekstrak air buah Eucalyptus alba yang dibuat pada suhu tinggi. Penelitian ini dilakukan untuk mengetahui potensi peredaman radikal bebas dan penghambatan proliferasi sel lestrai MCF-7 dari ekstrak P-100. Ekstrak P-100 dibuat dari buah Eucalyptus alba yang diekstraksi dengan pelarut aquadest pada temperatur 100 C dan dikeringkan pada temperatur 80 C hingga diperoleh ekstrak kering. Total kapasitas antioksidan ekstrak ditentukan sebagai aktivitas peredaman radikal bebas 1,1-difenil-2-pikril-hidrazil (DPPH). Sebagai pembanding digunakan ekstrak etanol 70% dan ekstrak etanol 80%. Uji potensi antikanker ekstrak P-100 dilakukan dengan uji sitotoksik terhadap sel lestari tumor payudara MCF-7 sebagai sel uji dengan metode 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyltetrazolium bromide (MTT). Total kandungan fenol ekstrak P-100 diukur secara spektroskopi dengan pereaksi Folin-Ciocalteu. Hasil penelitian menunjukkan bahwa ekstrak P-100 memiliki aktivitas antioksidan (IC50=18.25 ppm) yang lebih tinggi dibandingkan ekstrak etanol 70% (IC50 = 22.18 ppm) dan ekstrak etanol 80% (IC50 = 24.53 ppm). Ekstrak P-100 memiliki aktivitas menghambat proliferasi sel lestari tumor payudara MCF-7 sebesar 83.42% pada konsentrasi 50 ppm. Aktivitas antioksidan dan penghambatan proliferasi ekstrak P-100 diduga berkorelasi dengan kandungan fenol total. Fenol ekstrak P-100 (42.04 0.17 mg of GAE/g) diketahui lebih tinggi daripada ektrak etanol 70% (22.81 0.35 mg of GAE/g) dan ektrak etanol 80% (15.11 0.13 mg of GAE/g). Berdasarkan hasil penelitian ini dapat disimpulkan bahwa Ekstrak P-100 dari buah Eucalyptus alba memiliki aktivitas antioksidan dan antikanker.
Chemistry Notes, Jul 2, 2018
Research on toxicity test of Sarcophyton sp. extract from Timor against Artemia s. Leach Nauplii ... more Research on toxicity test of Sarcophyton sp. extract from Timor against Artemia s. Leach Nauplii has been conducted. Sarcophyton sp. from Paradiso beach, Kupang, was macerated in n-hexane, ethyl acetate, and methanol then each macerate was evaporated at 40 0 C and 45 0 C to dry. The dried extract resulted was tested its secondary metabolite content and toxicity using Brine Shrimp Lethality Test (BSLT). The results showed that the methanol extract contains saponins, phenols, and steroids while the ethyl acetate extract contains steroids and terpenoids. The toxicity test against artemia showed that the methanol extract from Sarcophyton sp. is very toxic to the nauplii with LC 50 value of 29,56 ppm while the ethyl acetate extract is classified toxic and gave LC 50 value of 34,11 ppm.
Rasayan Journal of Chemistry, 2021
Psidium guajava was abundantly found in arid land of Timor and traditionally used for treatment a... more Psidium guajava was abundantly found in arid land of Timor and traditionally used for treatment against diarhea. Research had been performed to analyze the chemical profiles together with antibacterial and fruit flies attractant activity of the leaf essential oil from P. guajava. The powder of dried leaves was subjected to hydro-distillation using Clevenger apparatus to obtain essential oil with a yield of 0.5%. Analysis of GC-MS spectra revealed that limone and β-caryophyllene were the main components of the essential oil. The essential oil showed strong antibacterial activity with the zone inhibition was observed at 16.3 for Staphyloccus aureus and 15.9 mm for Escherechia coli. On the other hand, no fruit flies were trapped during the field test in guava fruit.
Biologi Edukasi: Jurnal Ilmiah Pendidikan Biologi, 2021
Hospital liquid waste has the potential to pollute the environment and harm health if not process... more Hospital liquid waste has the potential to pollute the environment and harm health if not processed properly because it contains high enough organic and inorganic compounds, pathogenic microorganisms, toxic chemicals and radioactive substances. Rumah Sakit Umum Daerah (RSUD) Kefamenanu is the only hospital owned by the Government of Kabupaten Timor Tengah Utara and located in the middle of community settlements and uses wastewater treatment plants (IPAL) in the treatment of hospital liquid waste since 2016. The results of liquid waste quality examination showed that there are several parameters that have increased after processing through IPAL so that researchers use filtration method in processing liquid waste of RSUD Kefamenanu. This study aims to find out the effectiveness of liquid waste treatment of RSUD Kefamenanu through filtration process using filter media in the form of coconut coir, sand, palm oil, activated charcoal and zeolite. This research is a pseudo experiment, namely to find out the percentage of decreased levels of liquid waste parameters through the filtration process. The results showed that the levels after processing had an average value of BOD 3.86 mg/L, TSS 14 mg/L and ammonia of 0.10 mg/L. The filtration process was able to lower BOD levels by 63.62%, TSS by 93.24% and ammonia by 86.57%. Based on these results, it can be concluded that the filtration process is able to lower levels of BOD, TSS and ammonia in liquid waste of RSUD Kefamenanu.