pitri susanti | Universitas Udayana (original) (raw)

Papers by pitri susanti

Journal of Pharmaceutical Science and Application

Backgrounds: Cyanidin and peonidin, anthocyanin compounds, have many in silico, in vitro, and in ... more Backgrounds: Cyanidin and peonidin, anthocyanin compounds, have many in silico, in vitro, and in vivo activities, including antioxidant, anticancer, antihyperlipidemic, and antidiabetic. Toxicity testing is carried out to determine the potential hazard that may be produced by the test compound. Objective: This study was aimed to determine the in silico toxicity of anthocyanins (cyanidin and peonidin) using Toxtree v3.1.0 software. Methods: In silico toxicity testing was carried out using 2D structures of cyanidin and peonidin with Cramer rules, Verhaar scheme, Benigni/Bossa rulebase, Kroes TTC decision tree, Eye Irritation/Corrosion and Skin Irritation/Corrosion parameters. Data analysis on the results of the tested toxicity parameters was carried out descriptively. Results: The results showed that the two compounds have the same category for the toxicity parameters of Cramer Rules (class III), Kroes TTC Decision Tree (substance would not be expected to be safety concern), Benigni/B...

International Journal of Molecular Sciences, 2021

The G1 phase of cell cycle progression is regulated by Cyclin-Dependent Kinase 4 (CDK4) as well a... more The G1 phase of cell cycle progression is regulated by Cyclin-Dependent Kinase 4 (CDK4) as well as Cyclin-Dependent Kinase 6 (CDK6), and the acivities of these enzymes are regulated by the catalytic subunit, cyclin D. Cell cycle control through selective pharmacological inhibition of CDK4/6 has proven to be beneficial in the treatment of estrogen receptor-positive (ER-positive) breast cancer, particularly improving the progression-free survival of patients. Thus, targeting specific inhibition on CDK4/6 is bound to increase therapeutic efficiency. This study aimed to obtain CDK4/6 inhibitors through a pharmacophore-based virtual screening of the ZINC15 purchasable compound database using the in silico method. The pharmacophore model was designed based on the FDA-approved cdk4/6 inhibitor structures, and molecular docking was performed to further screen the hit compounds obtained. A total of eight compounds were selected based on docking results and interactions with CDK4 and CDK6, us...

Archives of Pharmacy Practice, 2012

Molecules, 2021

In cell development, the cell cycle is crucial, and the cycle progression’s main controllers are ... more In cell development, the cell cycle is crucial, and the cycle progression’s main controllers are endogenous CDK inhibitors, cyclin-dependent kinases (CDKs), and cyclins. In response to the mitogenic signal, cyclin D is produced and retinoblastoma protein (Rb) is phosphorylated due to activated CDK4/CDK6. This causes various proteins required in the cell cycle progression to be generated. In addition, complexes of CDK1-cyclin A/B, CDK2-cyclin E/A, and CDK4/CDK6-cyclin D are required in each phase of this progression. Cell cycle dysregulation has the ability to lead to cancer. Based on its role in the cell cycle, CDK has become a natural target of anticancer therapy. Therefore, understanding the CDK structures and the complex formed with the drug, helps to foster the development of CDK inhibitors. This development starts from non-selective CDK inhibitors to selective CDK4/CDK6 inhibitors, and these have been applied in clinical cancer treatment. However, these inhibitors currently req...

International Journal of Pharmacy and Pharmaceutical Sciences, 2017

Objective: The aim of this study was to observe the antidyslipidemic and antioxidant activity of ... more Objective: The aim of this study was to observe the antidyslipidemic and antioxidant activity of andrographolide (AND) compound from Sambiloto (Andrographis paniculata) herb. The antidyslipidemic activity could be described by lowering TC (total cholesterol), TG (triglyceride), and LDL (low-density lipoprotein) level in the blood and by increasing HDL (high-density lipoprotein) level in the blood. The antioxidant activity could be described by inhibiting the ox-LDL (oxidized low-density lipoprotein) formation.Methods: AND was separated from the sambiloto ethanolic extract. Atherosclerosis in rats was induced by rich fat food (5% yolk, 10% lard), 1% calcium and 20.000 IU vitamin D3 for 90 d. The rats were treated with AND compound on the 61st day for 30 d. On the 90th day, the blood of rats had been collected, and its blood vessel was also extracted. Antidyslipidemic activity was measured by estimating the level of TC, TG, LDL and HDL in the blood. Antioxidant activity was measured b...

Journal of Health Sciences and Medicine, 2017

Bitter herbs (Sambiloto) have many pharmacological activities including antioxidant, antidiabetic... more Bitter herbs (Sambiloto) have many pharmacological activities including antioxidant, antidiabetic, anticancer, antihyperlipidemic and anti-inflammation. Andrographolide is a diterpene compounds contained in bitter herbs. It is known that andrographolide compound responsible for the pharmacological activity of the bitter herbs. This study investigated DPPH free radical scavenging activity from andrographolide diterpene lactone. This study was initiated with the isolation of andrographolide compound from bitter herbs and then testing their DPPH free radical scavenging. The results suggested that andrographolide had IC50 value of 5.45 mg. This means andrographolide has 50% DPPH inhibition effect, i.e 5.45 mg.

Tomat ( Solanum lycopersicum L.) merupakan salah satu buah yang identik dengan warna merah menyal... more Tomat ( Solanum lycopersicum L.) merupakan salah satu buah yang identik dengan warna merah menyala. Warna merah tersebut disebabkan oleh adanya pigmen merah karotenoid. Karotenoid yang paling berlimpah dalam buah tomat adalah likopen sebesar 60-64%. Likopen memiliki aktivitas antioksidan tertinggi dibandingkan karotenoid lainnya. Berdasarkan hasil Survei Sosial Ekonomi Nasional (SUSENAS) periode 2010-2014 menunjukkan bahwa konsumsi rata-rata buah tomat di Indonesia lebih kecil bila dibandingkan dengan ketersediaan buah tomat dari Necara Bahan Makanan (NBM). Produksi buah tomat yang berlebih dan tidak dimanfaatkan secara efektif seringkali dapat menimbulkan masalah bagi lingkungan karena buah tomat yang membusuk dan akhirnya menjadi limbah. Dalam penelitian ini dilakukan ekstraksi likopen dari limbah buah tomat dengan metode ekstraksi cair-cair untuk mengetahui jumlah kadar total likopen dari limbah buah tomat. Limbah buah tomat yang digunakan adalah buah tomat yang telah mengalami p...

International Journal of Pharmacy and Pharmaceutical Sciences, 2016

Objective : The present study was to evaluate the anti dyslipidemic activity and the atherogenic ... more Objective : The present study was to evaluate the anti dyslipidemic activity and the atherogenic indicator of saponin compound from katuk leaves ( Sauropus androgynus (L). Methods : This compound separated from Katuk leaves crude extract by column chromatography (a mobile phase: silica GF 254; mobile phase = gradient polarity of solvent (chloroform: methanol = 1:9-9:1). Male Wistar rats were fed with fat-rich diet (15% pig fats, 5% duck egg yolk) for 30 d to increase its total cholesterol level, triglyceride, LDL and to decrease blood HDL level compared to normal lipid level. Saponins fraction of Katuk leaves dose 10 and 30 mg/kg BW was given for 2 w and then its lipid level in blood was measured. Lipid level difference from each group was analyzed statistically. Results : Total cholesterol, triglyceride, LDL and HDL level from a group of rats that were given saponins fraction of Katuk leaves was significantly different with negative control rats group and was not significantly diff...

Pharmaceutical care takes an essential role in order to realize the patient safety caring with ef... more Pharmaceutical care takes an essential role in order to realize the patient safety caring with effective medication cost. It was studied the pharmaceutical caring in pharmacy-clinic-Unit of Wangaye Hospital-Denpasar, which provided to the hospitalization patients of PT. Askes (Persero)-Cabang Denpasar and the role of the health insurance verification. Aim of the study was to observe the role of pharmacist and health insurance verification in order to realize the patient safety caring with effective medication cost. The pharmacist in this unit has strived for a pharmaceutical praxis according to the pharmaceutical regulation but not yet optimal utilized his praxis to obtain a rational use of drugs and effective medical cost. There was not a pharmacist, which was employed by PT ASKES to verify the pharmaceutical praxis, which provided pharmaceutical care to the ASKES patients.

Research Journal of Pharmacy and Technology, 2018

Excessive food consumption concerning to high calorie, dyslipidemia, obesity and cardiovascular d... more Excessive food consumption concerning to high calorie, dyslipidemia, obesity and cardiovascular disease. One alternative therapeutic approach in dyslipidemia patients is traditional use of herbal medicines such as Zingiber montanum rhizome. The aim of this study is to compare the anti-dyslipidemia activity of ethanol (EEZR), methanol (MEZR) and ethyl acetate extract of Zingiber montanum rhizome (EAEZR). EEZR, MEZR, and EAEZR were made by maceration method. The solvent extract was evaporated to obtain a viscous extract. Then a phytochemical screening for the compounds contained in each extract was performed. To induce dyslipidemia, Wistar male rats were given standard feed (80%), duck egg yolk (5%) and lard (15%) for 30 days, followed by administration of 1500 mg EEZR, MEZR and EAEZR dose for 30 days. On the 60th day, total cholesterol (TC), triglyceride (TG) and high-density lipoprotein (HDL) blood measurements of the test rat were performed. Phytochemical screening result showed that EEZR, MEZR, and EAEZR contained flavonoid and terpenoid compounds. EEZR, MEZR, and EAEZR could decrease TC, TG and increased HDL in mouse blood significantly compared with dyslipidemia group (p <0.05). The TC and TG percentage decrease and the highest HDL level were owned by ethyl acetate extract of Zingiber montanum rhizome. EEZR, MEZR and EAEZR could decrease TC, TG levels and increase HDL in rat blood. The ability to decrease TC and TG levels from Zingiber montanum rhizome extract could be caused by the EEZR ability to inhibit pancreatic lipase enzyme activity so that it could suppress the fat absorption from the rats’ small intestine. The EAEZR ability was best amongst other because the active compound contained in non-polar Zingiber montanum rhizome is 2-methoxy-8- (3,4-dimethoxyphenyl) -1,4napthoquinon. EEZR, MEZR, and EAEZR have the ability to anti-dyslipidemia, where the best activity was owned by EAEZR.

Metamorfosa: Journal of Biological Sciences, 2017

Phenol compound in Piper betle leaves has several pharmacology activities such as antibacteria, a... more Phenol compound in Piper betle leaves has several pharmacology activities such as antibacteria, antifungi and antioxidant. The pharmacology activities of a herbal drug are influenced by the phytochemistry content, so in order to do a quality determination that provides phytochemistry profile and consistent pharmacology activities, a standardization is required. Fingerprint is the main standard to perform quality control for herbal drug. TLC-spectrophotodensitometry was used as the method in order to provide fingerprint profile of phenol compound. In this experiment, Piper betle leaves samples were extracted by reflux method using ethanol 96% as the solvent. Identification of phenol compound was done using TLC-spectrophotodensitometry with Silica gel 60 F254 as the stationary phase, toluena: ethyl acetate (93:7 v/v) as the mobile phase, FeCl3 and Folin-Ciocalteau as the reagent. The fingerprint profile of phenol compund was shown in Rf value 0,19; 0,42; and 0,62. Positive results of ...

Jurnal Farmasi Udayana, 2017

Reactive Oxygen Species (ROS) merupakan suatu produk alami yang terbentuk dari metabolisme aerobi... more Reactive Oxygen Species (ROS) merupakan suatu produk alami yang terbentuk dari metabolisme aerobik normal dalam tubuh yang secara potensial dapat menyebabkan kerusakan. Penelitian bertujuan untuk mengetahui potensi antioksidan yang paling kuat dari ekstrak buah mengkudu (Morinda citrifolia L), ekstrak daun kelor (Moringa oleifera) dan ekstrak daun kedondong hutan (Spondias pinnata (l.f) kurz). Pengujian aktivitas antioksidan dari ketiga tanaman ini yaitu dilakukan dengan metode DPPH yang kemudian diamati dengan Spektro UV-Vis pada panjang gelombang maksimum 315 nm. Analisis data dilakukan dengan menghitung persen inhibisi (% inhibisi) yang kemudian dibuat persamaan regresi linear. Hasil yang diperoleh bahwa nilai IC50 ekstrak buah mengkudu yaitu 22,95 µg/mL, nilai IC50 dari ekstrak daun kelor yaitu 42,19 µg/mL sedangkan nilai IC50 dari ekstrak daun kedondong hutan yaitu 49,97 µg/mL

Jurnal Farmasi Udayana, 2013

Telah dilakukan validasi metode uji konfirmasi senyawa golongan benzodiazepin, yang meliputi spes... more Telah dilakukan validasi metode uji konfirmasi senyawa golongan benzodiazepin, yang meliputi spesifisitas, selektivitas, presisi, ketahanan (robustness), serta sensitivitas dengan HPLC-DAD. Penelitian ini bertujuan untuk memperoleh metode uji konfirmasi yang telah tervalidasi untuk senyawa golongan benzodiazepin. Penelitian dilakukan terhadap senyawa nitrazepam, bromazepam, flurazepam, klordiazepoksida, dan diazepam. Sistem kromatografi yang digunakan adalah fase diam kolom Luna C8-strong cation exchange dengan fase gerak campuran dapar fosfat 0,05 M pH 3 dan asetonitril 90% (68,5:31,5, v/v). Sistem ini menghasilkan pemisahan yang spesifik, selektif, dan seksama untuk semua senyawa uji, namun tidak senstitif dan tidak tahan (robust).

ejournal.unud.ac.id

... Ni Made Pitri Susanti1,2), Rahmana E. Kartasasmita2), Amir Musadad2), and Daryono H. Tjahjono... more ... Ni Made Pitri Susanti1,2), Rahmana E. Kartasasmita2), Amir Musadad2), and Daryono H. Tjahjono2) ... to the interaction between the two molecules, because are electrons more easily transferred from the occupied MOs of donor to the unoccupied MOs of acceptor (Fleming, 1976 ...

A research of identification of cannabinoid compounds of stems of cannabis sp was conducted. The ... more A research of identification of cannabinoid compounds of stems of cannabis sp was conducted. The aim of thisresearch was identified cannabinodes using TLC and HPTLC spectrophotodensitometry and confirmed by colortest. Cannabinoides of stems cannabis sp was extracted by maceration method by using ether as solvent.Concentrated extract was spotted on AL-TLC Si 60 G F 254 and also HPTLC Si 60 G F 254. Plates were elutedwith hexane-diethyl ether (80:20, v/v) in ascending on twin chember. Each peak was scanned on Camag TLCScannerIII, Identification of each insitu spectra were based on Rf and library spectra and confirmed by colortestby Fast Blue B salt.. The result showed that method can be used to identify the CBN, THC and CBD contentof marijuana stems.