Antioxidant Activity Test of Andrographolide in Bitter Herbs Using DPPH Scavenging (original) (raw)
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International Journal of Pharmacy and Pharmaceutical Sciences, 2017
Objective: The aim of this study was to observe the antidyslipidemic and antioxidant activity of andrographolide (AND) compound from Sambiloto (Andrographis paniculata) herb. The antidyslipidemic activity could be described by lowering TC (total cholesterol), TG (triglyceride), and LDL (low-density lipoprotein) level in the blood and by increasing HDL (high-density lipoprotein) level in the blood. The antioxidant activity could be described by inhibiting the ox-LDL (oxidized low-density lipoprotein) formation.Methods: AND was separated from the sambiloto ethanolic extract. Atherosclerosis in rats was induced by rich fat food (5% yolk, 10% lard), 1% calcium and 20.000 IU vitamin D3 for 90 d. The rats were treated with AND compound on the 61st day for 30 d. On the 90th day, the blood of rats had been collected, and its blood vessel was also extracted. Antidyslipidemic activity was measured by estimating the level of TC, TG, LDL and HDL in the blood. Antioxidant activity was measured b...
Journal of Food and Pharmaceutical Sciences, 2020
The main bitter constituents of sambiloto (Androgaphis paniculata (Burm., F) Ness) are diterpene lactones, namely andrographolide and deoxyandrographolide which have been reported to have antidiabetic, cytotoxic, antiatherosclerosis, anti-inflammatory, and antimicrobial activity. There are many studies that performed the isolation of deoxyandrographolide and andrographolide from A. paniculata herbs, but most of them included several steps that make them not efficient. This research was conducted to do an isolation of deoxyandrographolide and andrographolide through liquid-liquid fractionation (LLF) due to its simplicity, low cost, and time efficient. The extraction of deoxyandrographolide and andrographolide from the herbs was carried out using chloroform as the solvent by using Soxhlet apparatus, and LLF was performed to isolate the compounds. The identities of the compounds were confirmed by TLC scanner compared to its standard references. Hence, these present methods were success...
Activity-Guided Isolation of Antioxidant Compounds from Andrographis stenophylla Leaf
E Journal of Chemistry, 2010
The antioxidant potency of various extracts of Andrographis stenophylla leaf was evaluated in vitro using ferric thiocyanate method. Reductive ability and free radical scavenging activity of the extracts were also investigated. Amounts of phenolic compounds in each of the extracts were determined using Folin-Ciocalteau reagent and compared to observe the correlation between antioxidant activities and total phenolic content. Methanol extract exhibited maximum antioxidant activity and was found to contain 2% of total phenolic compounds. Methanol extract was subjected to column chromatographic separation over silica gel G using ethyl acetate: formic acid: acetic acid: water. Fractions thus obtained were screened for their antioxidant activity. Among the eleven fractions screened, fraction C was more active than the standard butylated hydroxyanisole. Fraction C on further fractionation with n-butanol: acetic acid: water afforded two flavanoids namely acacetine and isosakuranetine. Fraction A was also shown to possess good antioxidant activity which was developed using TLC and indicated the presence of a terpenoid, Andrographolide. The structures of the isolated compounds were confirmed by UV, IR, MS, 1 H and 13 C NMR spectral data. This is the first report wherein Andrographolide, Acacetine and Isosakuranetine are isolated from Andrographis stenophylla leaf.
Pak J Bot, 2011
The active parts of 11 medicinal plants were analyzed for physico-chemical evaluation, phytochemical determination and antioxidant activity. The physico-chemical evaluation revealed that highest water soluble extractive was from Origanum vulgare (38%), highest chloroform extractive was from Psoralea corylifolia (21%); highest ethanolic extractive was that of Acorus calamus (11%) and the highest hexane extractive value was for Arnebia nobilis (9.8%). The total ash content evaluation indicated that Achillea millefolium yielded (20.2 %) and Rauvolfia serpentina yielded (41.6%); these values are much higher than the standard ash values for these plants indicating that these drugs are highly adulterated and substandard. The highest essential oil was yielded by Acorus calamus (3.2%). The highest saponin percentage was analyzed in Acorus calamus (8.9%), while the alkaloids percentage was determined at 21% in Peganum harmala. Among all the plants assessed for DPPH free radical scavenging activity, the maximum activity was shown by Paeonia emodi (85.8%), followed by Achillea millefolium (81.7%) and Origanum vulgare (80.3%).
IN VITRO ANTIOXIDANT POTENTIAL OF WHOLE PLANT OF ANDROGRAPHIS ECHIOIDES (L.) NEES (ACANTHACEAE
International Journal of Pharmacy and Pharmaceutical Sciences, 2016
Objective: Antioxidant activity of petroleum ether, benzene, ethyl acetate, methanol and ethanol extracts of the whole plant of Andrographis echioides have been tested using various antioxidant model systems viz; DPPH (1,1-Diphenyl-2-picryl-hydrazyl), hydroxyl, superoxide, ABTS (2, 2'-Azino-Bis-3-Ethylbenzothiazoline-6-Sulfonic Acid) and reducing power. Methods: Total phenolic content was estimated by folin-ciocalteau method. Flavonoids were determined by Aluminium chloride method. In vitro antioxidant activity of petroleum ether, benzene, ethyl acetate, methanol and ethanol extracts was evaluated by studying DPPH (1,1-Diphenyl-2picryl-hydrazyl) radical scavenging activity, hydroxyl radical scavenging activity, superoxide radical scavenging activity, ABTS radical cation scavenging activity and reducing power using the standard procedure. Results: The total phenolic and flavonoids in methanol extract were found to be 1.26g/100 g and 1.18 g/100 g respectively. The methanol extract of Andrographis echioides is found to possess DPPH, hydroxyl, superoxide and ABTS radical cation scavenging activity. The IC50 value of DPPH, hydroxyl, superoxide, and ABTS radical cation scavenging activity were found to be 49.11, 36.18, 37.13 and 38.15 μg/ml respectively. Like the antioxidant activity, reducing the power of the extract increases with increase in concentration. Conclusion: The finding indicated promising antioxidant activity of crude extracts of the above plant and needs further exploration for their effective are in the both modern and traditional system of medicines.
Assessment of DPPH free radical scavenging activity of some medicinal plants
2013
Free radicals are one of the critical causative factors in the pathogenesis of many neurodegenerative disorders, autoimmune diseases, inflammatory conditions, diabetes, and gastrointestinal disorders. Several phytochemical and biological studies have made it evident that medicinal plants have profound antioxidant potentials that can be furnished further in the prevention and treatment of these annihilating diseases. We had assessed ethanol extracts of 08 medicinal plants from Bangladesh, for antioxidant potentials with the help of DPPH (1,1-diphenyl-2-picryl hydrazyl) free radical scavenging assay. Those plant extracts were found to have profound antioxidant effects that could explain and justify some of their uses in traditional medicine. Among the extracts, Ammannia multiflora, Caesalpinia pulcherrima, Dendrophthoe falcata and Syzygium cumini were observed displaying strong DPPH free radical scavenging action with IC50 value of 6.25 µg/ml, 7.46 µg/ml, 6.22 µg/ml, 6.08 µg/ml respec...
Andrographolide, A Natural Antioxidant: An Update
Antioxidants
Traditionally, Andrographis paniculata has been used as an herbal remedy for lung infection treatments. Its leaves contain a diterpenoid labdane called andrographolide responsible for a wide range of biological activities such as antioxidant, anti-inflammatory, and anti-cancer properties. This manuscript is a brief review of the antioxidant mechanisms and the regulation of the Nrf2 (nuclear factor (erythroid-derived 2)-like 2) signaling pathway by andrographolide.
Screening of various botanical extracts for antioxidant activity using DPPH free radical method
African Journal of Traditional, Complementary and Alternative Medicines, 2013
Aiming at the exploration of herbal use by society, crude extracts of the seeds of some commonly used medicinal plants (Vitis vinifera, Tamarindus indica and Glycin max) were screened for their free radical scavenging properties using ascorbic acid as standard antioxidant. Free radical scavenging activity was evaluated using 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical. The overall antioxidant activity of grape seeds (Vitis vinifera) was the strongest, followed in descending order by soybean (Glycin max) and tamarind (Tamarindus indica). The seeds extract of Vitis vinifera, Glycin max and Tamarindus indica showed 85.61%, 83.45% and 79.26%, DPPH scavenging activity respectively.
Journal of Intercultural Ethnopharmacology, 2018
Background/Aim: The medicinal plants and their derivatives have long been recognized as important sources of antioxidants in the prevention and treatment of various diseases. This study investigated phytochemicals, antioxidant content and in vitro DPPH free radical scavenging activities of methanolic and aqueous leaves extracts of Anogeissus leiocarpus, Ipomoea asarifolia, Bauhinia rufescens, Guiera. senegalensis and Moringa oleifera. Materials and Methods: The extracts were subjected to qualitative phytochemical analysis to identify the bioactive constituents in each plant. Total phenolics and proanthocyanidin contents were determined using FolinCiocalteu and vanillin-methanol assays while antioxidant free scavenging activity was estimated using 2,2-diphenyl-1-picrylhydrazyl (DPPH) assay. Results: The phytochemical screening revealed the presence of alkaloids, flavonoids, and tannins in all the five plants investigated. The total phenolic and proanthocyanidin contents of methanoli...