Daniel Redoules | Université Paul Sabatier de Toulouse (original) (raw)

Papers by Daniel Redoules

Skin Pharmacology and Physiology, 2008

Atopic dermatitis (AD) is a multifactorial chronic inflammatory disease mainly stemming from a ge... more Atopic dermatitis (AD) is a multifactorial chronic inflammatory disease mainly stemming from a genetic predisposition that leads to hypersensitivity to environmental factors and a common involvement of Staphylococcus aureus (SA) colonization. The aim of this work was to propose a new non-invasive approach to enumerate the genes coding for the toxins of SA in atopic skin samples. In parallel, the study aimed to evaluate the change in AD through 3 markers of the inflammatory response: IL-8, IL-1RA/IL-1α and IL-18. These methods were tested on 31 patients with AD, and finally on a group of 19 subjects for whom clinical improvement had been reported after various treatments. The study revealed the presence of a large number of genes encoding toxins in atopic samples, indicating a high rate of SA colonization, and also an increase in the level of all cytokine markers in atopic skin compared to the skin of healthy subjects. Finally, we found a positive correlation between increases in the...

The present invention relates to Formula (I), wherein - if X = NH, each of R1, R2, R3, R4 represe... more The present invention relates to Formula (I), wherein - if X = NH, each of R1, R2, R3, R4 represents a hydrogen atom; if X = N, the core is aromatic, and R2, R3 and R4 represent hydrogen atoms, and R1 = H, halogen, aryl, heteroaryl, alkenyl or acetylenyl; or R1, R2, R3, R4 are defined such that R1 or R2 or R3 or R4 represents a methyl group, and the three other radicals represent a hydrogen atom.

Journal of Allergy and Clinical Immunology, 2016

Sciences (grant no. UL1TR000039), and Arkansas Biosciences Institute (to R.C.K.). S.S.A has salar... more Sciences (grant no. UL1TR000039), and Arkansas Biosciences Institute (to R.C.K.). S.S.A has salary support from CEGIR (U54 AI117804) which is part of the Rare Diseases Clinical Research Network (RDCRN), an initiative of the Office of Rare Diseases Research (ORDR), NCATS, and is funded through collaboration between NIAID, NIDDK, and NCATS, funded by AI114585 (to T.A.D.). Disclosure of potential conflict of interest: E. Tkachenko owns stock in MuWells Inc (supplier of elastic cell substrates

The Journal of investigative dermatology, 2005

A new strategy for the skin delivery of bioactive compounds has been developed, using enzymes inv... more A new strategy for the skin delivery of bioactive compounds has been developed, using enzymes involved in the maintenance of the epidermal barrier function and the enzymatic transformation of corresponding precursors. This new strategy has been tested with regard to two enzymatic activities of the skin barrier: extracellular glucosidase and esterase/lipase. An analysis of the requirements for the glycosidic bond hydrolysis of any glycoconjugate by beta-glucocerebrosidase indicates that the release of the moiety linked to the glucose unit is obtained as long as the glycosidic bond being broken is not hindered, and as long as the leaving group property of the released moiety is good enough. This strategy was first applied to the release of the antioxidant delta-tocopherol. It was then extended to retinoic acid by introducing a spacer between the glucose unit and the bioactive moiety. This spacer was either a good leaving group such as hydroquinone, or a structure akin to a ceramide, n...

The present invention relates to a topical composition, cosmetic or dermatological composition, c... more The present invention relates to a topical composition, cosmetic or dermatological composition, comprising a diglyceride 1.3 of general formula (I): wherein the radicals R1 and R2 represent, independently of one another, a saturated alkyl radical and linear C13 to C40, and at least one cosmetic or dermatological excipient for topical application to the skin. The present invention also relates to such a composition, or a diglyceride 1.3 of formula (I), for use in the fight against embrittlement of the cutaneous barrier and in particular to prevent or reduce the penetration of exogenous molecules in the skin following an embrittlement of the skin barrier.

La presente invention propose un complexe glucosyle ternaire, bioprecurseur d'au moins un pri... more La presente invention propose un complexe glucosyle ternaire, bioprecurseur d'au moins un principe actif retinoique, destine a une application percutanee, de formule (I) dans laquelle: E represente un groupement espaceur hydrocarbone lineaire, ramifie ou cyclise, de caractere aliphatique ou aromatique pouvant contenir un ou plusieurs heteroatome(s) d'oxygene et pouvant porter un ou plusieurs groupe(s) carbonyle, A represente un reste d'une molecule dudit principe actif retinoique, lie au groupement espaceur par une fonction carboxylate, n = 1 ou 2.

L'invention concerne un dispositif (1) de mesure des proprietes physicochimiques de la peau (... more L'invention concerne un dispositif (1) de mesure des proprietes physicochimiques de la peau (8), notamment son pH et son etat d'oxydation, comportant un multipotentiostat (9) de generation, de regulation et de traitement de tensions electriques, relie a des moyens (2) de mesure de proprietes de la peau (8), caracterise en ce que les moyens de mesure comportent au moins deux microelectrodes (4) de travail et une microelectrode (56) de reference, chaque microelectrode comportant un tube capillaire isolant electrique a l'interieur duquel s'etend un fil metallique dont l'extremite libre (7) forme un disque apte a etre mis en contact direct avec la peau lors des mesures. Elle concerne egalement un procede d'utilisation du dispositif de mesure.

The present invention provides a ternary glucosyl complex, bioprecursor of at least one retinoic ... more The present invention provides a ternary glucosyl complex, bioprecursor of at least one retinoic active principle, intended for percutaneous application, of formula (I) (CF DRAWING IN BOPI) wherein: - E represents a linear hydrocarbon spacer group, branched or cyclized aliphatic or aromatic character which can contain one or more heteroatom (s) and oxygen may bear one or more group (s) carbonyl, - a represents a residue of a molecule of said retinoic active principle, linked to the spacer group by a carboxylate functional group, - n = 1 or 2.

Experimental Dermatology

Background: Although acne vulgaris has a multifactorial aetiology, comedogenesis and bacteria col... more Background: Although acne vulgaris has a multifactorial aetiology, comedogenesis and bacteria colonization of the pilosebaceous unit are known to play a major role in the onset of inflammatory acne lesions. However, many aspects remain poorly understood such as where and when is the early stage of the Propionibacterium acnes colonization in follicular unit? Our research aimed at providing a precise analysis of microcomedone's structure to better understand the interplay between Propionibacterium acnes and follicular units, and therefore, the role of its interplay in the formation of acne lesions. Methods: Microcomedones were sampled using cyanoacrylate skin surface stripping (CSSS). Their morphology was investigated with multiphoton imaging and their ultrastructure with scanning electron microscopy (SEM) and transmission electron microscopy (TEM). Bacterial lipase activity in the microcomedones was quantified using a dedicated enzymatic test as well as a Fourier Transform Infra-Red (FTIR) analysis. The porphyrin produced by bacteria was analysed with HPTLC and fluorescence spectroscopy. Results: The imaging analysis showed that microcomedones' structure resembles a pouch, whose interior is mostly composed of lipids with clusters of bacteria and whose outer shell is made up of corneocyte layers. The extensive bacteria colonization is clearly visible using TEM. Even after sampling, clear lipase activity was still seen in the microcomedone. A high correlation, r = .85, was observed between porphyrin content measured with HPTLC and with fluorescence spectroscopy. These observations show that microcomedones, which are generally barely visible clinically, already contain a bacterial colonization.

Journal of Investigative Dermatology, 2016

Human T-lymphotropic virus type 1 (HTLV-1) infects between 5 and 10 million people worldwide. It ... more Human T-lymphotropic virus type 1 (HTLV-1) infects between 5 and 10 million people worldwide. It causes the aggressive malignancy known as Adult T-cell Leukemia/Lymphoma (ATL), HTLV-1-Associated Myelopathy/Tropical Spastic Paraparesis (HAM/TSP), and infective dermatitis. Although strong immune responses are generated against the virus, they do not eliminate HTLV-1. HTLV-1 propagates within and between individuals via cell-to-cell transmission. Here we demonstrate that the contact formed between a dendritic cell (DC) and an HTLV-1-infected T cell can reduce dendritic cell (DC) immunological functions by reducing TLR responses, IL-12 p40/p70 expression and MHC-II expression on the DC. These DC-T cell contacts induce spleen tyrosine kinase-mediated c-Raf/MEK/ERK signaling pathway activation on DCs but are independent of DC-SIGN signalling. Furthermore, both Alu-PCR and highthroughput integration site analysis of HTLV-1 show little evidence of integrated proviruses in DCs. Finally, we show that DCs can re-transfer HTLV-1 to target autologous CD4 + T-cells in trans. These findings suggest that DC/T-cell virological synapses contribute not only to viral cell-to-cell transmission in trans, but also to down-modulation of host innate and adaptive immunity against HTLV-1. Restoring DC functions in HTLV-1 infection might improve the early immune control of the virus and reduce the risk of emergence of ATL and other diseases including infective dermatitis.

Cheminform, 2010

ABSTRACT ChemInform is a weekly Abstracting Service, delivering concise information at a glance t... more ABSTRACT ChemInform is a weekly Abstracting Service, delivering concise information at a glance that was extracted from about 100 leading journals. To access a ChemInform Abstract of an article which was published elsewhere, please select a “Full Text” option. The original article is trackable via the “References” option.

Annales de Dermatologie et de Vénéréologie, 2012

Les malades ayant une insuffisance rénale chronique (IRC) ont des manifestations cutanées variées... more Les malades ayant une insuffisance rénale chronique (IRC) ont des manifestations cutanées variées dont le prurit.

Journal of Dermatological Science, 2013

The effects of hyaluronic acid (HA) injection on tissue collagen anabolism are suggested to be re... more The effects of hyaluronic acid (HA) injection on tissue collagen anabolism are suggested to be related to the induction of mechanical stress, causing biochemical changes in skin physiology. To ascertain the association between dermal mechanics modulated by a hyaluronic acid-based filler effect and metabolism. Sixty females were randomised to receive a 0.5mL injection of HA gel or isotonic sodium chloride (control) in the arm. Skin biopsies were taken at baseline and after 1, 3 and 6 months. Protein and gene expression of procollagen, matrix metalloproteinases (MMP) and MMP tissue inhibitors (TIMP1) were measured blind by ELISA and qPCR, respectively. Injected volumes were measured by high-frequency ultrasound and radiofrequency analysis. Skin layer effects of injections were analysed by finite element digital modelling. One month after injection, the filler induced an increase in procollagen (p=0.0016) and TIMP-1 (p=0.0485) levels and relative gene expression of procollagen III and I isoforms compared with the controls. After 3 months, procollagen levels remained greater than in the controls (p=0.0005), whereas procollagen expression and TIMP-1 and MMP content were no longer different. Forty-three percent of the injected filler volume was found at 1 month, 26% after 3 months and 20% after 6 months. The ultrasound imaging technique limited the scope of the investigation and precluded an evaluation of the action of the filler at the hypodermic level. Integrating both mechanical and biological aspects, our results suggest that mechanical stress generated by cross-linked HA plays a role in dermal cell biochemical response.

Journal of Allergy and Clinical Immunology

Frontiers in Immunology

Acne is a multifactorial disease driven by physiological changes occurring during puberty in the ... more Acne is a multifactorial disease driven by physiological changes occurring during puberty in the pilosebaceous unit (PSU) that leads to sebum overproduction and a dysbiosis involving notably Cutibacterium acnes. These changes in the PSU microenvironment lead to a shift from a homeostatic to an inflammatory state. Indeed, immunohistochemical analyses have revealed that inflammation and lymphocyte infiltration can be detected even in the infraclinical acneic stages, highlighting the importance of the early stages of the disease. In this study, we utilized a robust multi-pronged approach that included flow cytometry, confocal microscopy, and bioinformatics to comprehensively characterize the evolution of the infiltrating and resident immune cell populations in acneic lesions, beginning in the early stages of their development. Using a discovery cohort of 15 patients, we demonstrated that the composition of immune cell infiltrate is highly dynamic in nature, with the relative abundance ...