In vitro anti-Trichomonas vaginalis activity of Pistacia lentiscus mastic and Ocimum basilicum essential oil (original) (raw)
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The effect of some plants extracts on Trichomonis Vaginalis
Th'ts is an experimental study aimed to study the effect of three plants extracts on the activity of the Trichomonis vaginalis parasite. After isolation of the parasite and growing on CPLM media, six plant extracts three of them were water extracts and others were alcoholic extracts which were prepared from Artemisia herba alba, Punica grantum and Capparis spinosa respectively were added to parasite media. Study of parasite activity and grorvth after24,48,96,72 hours were observed. the Artemisia herba alba 'alcoholic extract had good suppression effect through 24 hours at 0.5'olo concentration while the same extract from Ptmica granatum at 0.5Yo concentration also affected the parasite through 48 hours and Capparis spinosa alcoholic extract inhibited the parasite activity in l%o concentration through 48 hours .The water extract for Artcmisia herba alba had better inhibition elfect through 48 hours at 0.5Yo concentration .Punica granatum affected through 24 hours but at l7o concentration, while Capparis water extract had no effect. We concluded that Artemisia herba a/64 alcoholic extract had better eflfect than others and rve recommended further studies to extract the active substances of medical plants for the treatment of Trichomonas vaginalis. 4+!r4ll drlJs*Lll s+$L "J" ASqll clt alii,,,qJl &t+ J1:il -:Ualii,r.All "
Effect of Mexican medicinal plant used to treat trichomoniasis on Trichomonas vaginalis trophozoites
Journal of Ethnopharmacology, 2007
Crude methanolic extracts from 22 Mexican medicinal plants were screened for antitrichomonal activity against Trichomonas vaginalis, which is the etiological agent of trichomoniasis. Among the plants tested Carica papaya and Cocos nucifera showed the best antitrichomonal activity with IC 50 values of 5.6 and 5.8 g/ml, respectively. The extracts of Bocconia frutescens, Geranium mexicanum, and Lygodium venustum showed moderate activity with IC 50 values ranging from 30.9 to 60.9 g/ml. All the other plant extracts were inactive (IC 50 > 100 g/ml). All extracts tested were less active than metronidazole (IC 50 0.037 g/ml), an antiprotozoal drug used as positive control. The results of the antiprotozoal screening support the popular uses of five of the plants tested for the treatment of some urogenital tract disorders in Mexican traditional medicine. However, seeds of Carica papaya and aerial parts of Bocconia frutescens should be used in herbal medicine with care to avoid toxicity.
Journal of Parasitic Diseases, 2014
Trichomonas vaginalis is a parasitic protozoan that is the aetiological agent of trichomoniasis, the most common non-viral sexually transmitted disease worldwide. Currently, the compound of choice for the treatment of T. vaginalis infections is metronidazole, however, it has many side effects and an increase in metronidazole-resistant trichomoniasis has been observed. Medicinal plants could be a source of new antiprotozoal drugs with high activity, low toxicity and lower price. The present work was carried out to investigate the therapeutic potential of Nigella sativa alcoholic extract and oil, as well as Phaseolus vulgaris (kidney bean) lectin and their in vitro activity on the ultrastructure of T. vaginalis trophozoites in comparison to metronidazole, as detected by transmission electron microscope. Both N. sativa oil and P. vulgaris lectin showed high toxic effect as evidenced by severe cell damage with cytoplasmic and nuclear destruction, while the effect of N. sativa alcoholic extract was moderate. Therefore, these two extracts could offer an effective, cheaper and more safe alternative for metronidazole in treatment of trichomoniasis.
Assessment of the Impact of Numerous Medicinal Plants on Trichomonas Vaginalis: A Review
Indian Scientific Journal Of Research In Engineering And Management, 2024
The most common non-viral sexually transmitted illness in the world, trichomoniasis, is caused by the etiological agent Trichomonas vaginalis. Trichomoniasis is a prevalent, global health issue that is becoming worse. While infections in males are typically asymptomatic, infections in the female genital tract can result in a variety of symptoms, including vaginitis and cervicitis. This condition has historically been underdiagnosed and under researched due to its generally mild symptoms and the absence of any evidence for any major repercussions. The attempts to identify and treat patients harbouring this parasite have risen, however, in light of mounting evidence that T. vaginalis infection is linked to various disease states with high morbidity in both men and women. Recent research has highlighted the complex interactions between the parasite and host, commensal microbiota, and associated symbionts. The pathophysiology of trichomoniasis is produced by damage to the host epithelia, mediated by a multitude of events during infection. The number of accessible diagnostic alternatives has increased as a result of the commercial introduction of several nucleic acid amplification tests (NAATs). Immunoassay based Point of Care testing is currently available, and a recent initial evaluation of a NAAT Point of Care system has given promising results, which would enable testing and treatment in a single visit.
Journal of Obstetrics, Gynecology and Cancer Research | ISSN, 2020
Trichomonas vaginitis is the most common non-viral disease that is transmitted sexually. Although metronidazole is the most effective medication for this disease, it has some adverse effects. Herbal medicines have fewer side effects and are more popular among people. This study aimed to review the plants, which are effective against Trichomonas vaginalis and their therapeutic effects. For this purpose, the databases of Scopus, PubMed, Medline, Google Scholar, SID, and Magiran were searched for the articles published during 2000-2019 by keywords, such as Trichomonas vaginalis, complimentary, complementary therapies, herbal medicine, medicinal plants, and their Persian equivalents. All in vivo and in vitro trial studies were included in the present review. The data indicated that some compounds, including eucalyptus, case, and garlic were effective in inhibiting the growth of T. vaginalis.
Iranian Journal of Pharmacology and Therapeutics, 2019
Coriandrum sativum has been used in Iranian traditional medicine as an antiinflammatory, antioxidant, antibacterial, and antifungal agent. The purpose of this study was to evaluate the chemical composition and anti-parasitic property of essential oil of C. sativum leaf on trophozoite of Trichomonas vaginalis. C. sativum was collected from Kermanshah city and essential oil was prepared by the Clevenger device. The essential oil was analyzed by GC/MS. Trophozoite of T. vaginalis was cultured in vitro in CPLM medium and the effect of the essential oil on the survival of T. vaginalis trophozoite was measured by Neobar slide. This study indicated that Linalool (71.2%) was the most constituent found in C. sativum essential oil. Also, the results of anti-parasitic tests demonstrated the concentrations of 0.5, 0.25, 0.125, 0.062, 0.031, 0.015, 0.007, 0.003, and 0.001 g/ml in essential oil and 0.25 g/ml in metronidazole could destroy of T. vaginalis trophozoite completely after 420 minutes i...
Journal of Parasitic Diseases, 2014
Trichomoniasis, the disease caused by the flagellate protozoan Trichomonas vaginalis is the sexually transmitted infection with the largest annual incidence. Metronidazole is the drug of choice recommended for the treatment of human trichomoniasis but it can lead to drug resistance and many other adverse effects. So, it is necessary for new alternatives for the treatment of this infection. Medicinal plants or herbs could be good alternative regimens to be inexpensive, effective and safe to use. In the present study, the therapeutic potential of Nigella sativa aqueous and alcoholic extracts as well as seeds oil was examined. Different concentrations of these plant preparations were incubated in vitro with cultivated T. vaginalis trophozoites and its effect on growth was compared with metronidazole under the same conditions. Both the alcoholic extract and oil proved to be valuable agents as efficient as metronidazole in treating T. vaginalis infection. The remarkable effect of N. sativa oil may be attributed to the fact that the active principles extracted from N. sativa seeds are mostly from its essential oil (omega 3, 6, 9 as well as 7 fatty acids). However, further experimental and clinical investigations are needed to evaluate and standardize the doses of these natural products to be safe and efficient.
Activity of Various Essential Oils Against Clinical Dermatophytes of Microsporum and Trichophyton
Frontiers in Cellular and Infection Microbiology, 2020
Dermatophytoses account for nearly a quarter of all fungal infections worldwide. These difficult to treat infections of the skin, hair, and nails, are growing more resistant to conventional antifungal treatments, and when treatable, often require prolonged therapeutic regimens. For centuries, essential oils have been used to treat a variety of ailments. In this study, we evaluated the clinical effects in vitro of 65 essential oils and 21 essential oil blends against various clinical species/strains of dermatophytes from two primary genera, Microsporum and Trichophyton. Our aim: To determine the overall activity of a wide range of essential oils against a number of clinical strains of dermatophytes. For all assays, 16 clinically derived species/strains of dermatophytes were used. The activity of each essential oil was assessed using a modified disk-diffusion assay over a period of 21 days of incubation vs. standard antifungal drugs. Subsequently, we determined the minimum inhibitory dilution possible for the most potent essential oils and performed combination testing to determine if synergy could be demonstrated with sub-inhibitory concentrations. We also assessed the effect of repeated vs. single applications. Of all the essential oils tested, cassia, cilantro, cinnamon, thyme, and oregano were the most potent along with one blend, DDR Prime; all genera/species tested were completely inhibited for 21 days following a single application. Many of the other oils tested exhibited temporal differences in activity where significant inhibition was observed ≤10 days of incubation which declined by day 21. Synergistic combinations were achieved with oregano and cilantro, cassia, or cinnamon bark; rose and cassia were also synergistic. Repeat application maintained complete inhibition for citronella, lemon myrtle, and litsea out to 21 days, but not lemon grass or On Guard. More study is necessary to understand the ways essential oils inhibit the growth of dermatophytes. Comprehensive research aimed at understanding the mechanism of action of essential oils and their components may provide the basis for a natural alternative to topical antifungal drugs. Such research could be envisioned to target optimal combinations and determine the timing between applications to provide for maximum inhibition of recurrence or growth.
2015
Article history: Received 31 April 2015 Accepted 19 May 2015 Available online 1 June 2015 Trichomoniasis is one of the most common urinary-genital tract disease. The causal agent of trichomoniasis is flagellate protozoan called Trichomonas vaginalis. Metronidazole and tinidazole are the drug of choice for trichomoniasis treatment. However, carcinogenic and embryo-teratogenic effects of the mentioned drugs and resistance to metronidazole has been reported. According to cytotoxic effects of medicinal herbs including Mentha longifolia and Pimpinella anisum on microbial organisms and their traditional use in sexually transmitted diseases the in vitro activity of the Mentha longifolia and Pimpinella anisum against a clinical strain of Trichomonas vaginalis was investigated. Concentrations of aqueous extract of Mentha longifolia leave (100-250 µg/ml) and Pimpinella anisum seed (100-500 µg/ml) were obtained. Trichomonas vaginalis isolated from patient with urogenital complications was cult...
In vitro Effect of Pomegranate Peel Extract on Trichomonas tenax
Abstract: The incidence of Trichomonas tenax (T. tenax) in patients with acute ulcerative gingivitis has been demonstrated in several published reports. Metronidazole was known as the most effective drug for human trichomoniasis, however, drug resistance and toxicity appeared. This study was designed in vitro to investigate the inhibitory activity of Punica granatum (P. granatun) ethanol extract on the growth and motility of T. tenax in comparison to metronidazole. Pomegranate ethanol extract group was treated with concentrations of 12.5, 25, 50, and 100 μg/ml. Metronidazole group and blank control were included. At 12 h, 24 h 48 h and 72 hr after drug treatment, the anti-T. tenax effect of pomegranate ethanol extract was tested by microscope counting method. The results showed 60% motility of T.tenax trophozoite after treatment with 12.5μg/100 ml of pomegranate ethanol extract group and 25 μg/ml showed higher anti-T. tenax (P<0.01). The ethanol extract of pomegranate peel has a remarkable effect on T. tenax, and among the groups, 60% ethanol extract shows the best anti-T. tenax activity. [Gehad T. El-Sherbini and Nahla M. Shoukry In Vitro effect of pomegranate peel extract on Trichomonas tenax] Life Science Journal 2012; 9(3):791-797]. (ISSN: 1097-8135). http://www.lifesciencesite.com. 113 Keywords: Trichomonas tenax, Punica granatum, Metronidazole, Herbal medication