Effect of Nigella sativa alcoholic extract and oil, as well as Phaseolus vulgaris (kidney bean) lectin on the ultrastructure of Trichomonas vaginalis trophozoites (original) (raw)
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Journal of Parasitic Diseases, 2014
Trichomoniasis, the disease caused by the flagellate protozoan Trichomonas vaginalis is the sexually transmitted infection with the largest annual incidence. Metronidazole is the drug of choice recommended for the treatment of human trichomoniasis but it can lead to drug resistance and many other adverse effects. So, it is necessary for new alternatives for the treatment of this infection. Medicinal plants or herbs could be good alternative regimens to be inexpensive, effective and safe to use. In the present study, the therapeutic potential of Nigella sativa aqueous and alcoholic extracts as well as seeds oil was examined. Different concentrations of these plant preparations were incubated in vitro with cultivated T. vaginalis trophozoites and its effect on growth was compared with metronidazole under the same conditions. Both the alcoholic extract and oil proved to be valuable agents as efficient as metronidazole in treating T. vaginalis infection. The remarkable effect of N. sativa oil may be attributed to the fact that the active principles extracted from N. sativa seeds are mostly from its essential oil (omega 3, 6, 9 as well as 7 fatty acids). However, further experimental and clinical investigations are needed to evaluate and standardize the doses of these natural products to be safe and efficient.
The effect of some plants extracts on Trichomonis Vaginalis
Th'ts is an experimental study aimed to study the effect of three plants extracts on the activity of the Trichomonis vaginalis parasite. After isolation of the parasite and growing on CPLM media, six plant extracts three of them were water extracts and others were alcoholic extracts which were prepared from Artemisia herba alba, Punica grantum and Capparis spinosa respectively were added to parasite media. Study of parasite activity and grorvth after24,48,96,72 hours were observed. the Artemisia herba alba 'alcoholic extract had good suppression effect through 24 hours at 0.5'olo concentration while the same extract from Ptmica granatum at 0.5Yo concentration also affected the parasite through 48 hours and Capparis spinosa alcoholic extract inhibited the parasite activity in l%o concentration through 48 hours .The water extract for Artcmisia herba alba had better inhibition elfect through 48 hours at 0.5Yo concentration .Punica granatum affected through 24 hours but at l7o concentration, while Capparis water extract had no effect. We concluded that Artemisia herba a/64 alcoholic extract had better eflfect than others and rve recommended further studies to extract the active substances of medical plants for the treatment of Trichomonas vaginalis. 4+!r4ll drlJs*Lll s+$L "J" ASqll clt alii,,,qJl &t+ J1:il -:Ualii,r.All "
Background: Trichomoniasis is the most common nonviral sexually transmitted human disease that is caused by protozoan Trichomonas vaginalis. Metronidazole is the selective drug in trichomoniasis treatment. However, the reported cases show an increasing trend of drug resistance. This study aimed to evaluate the effect of mango and blueberry extracts on T. vaginalis. Materials and Methods: T. vaginalis was cultured axenically in TYM (Trypticase Yeast Extract) medium supplemented with 10% bovine serum. The effect of mango and blueberry extracts at 50, 100, 200, 400, 800 and 1000 μg.mL-1 on T. vaginalis was studied after 24 and 48 hours. The final numbers of parasite with a hemocytometer and Trypan blue were recorded. Then the value of IC50 [Half maximal inhibitory concentration] and the lethal percent were calculated. In the present study, the metronidazole was used as positive control. The IC50 value of metronidazole and tinidazole were calculated in the concentrations of 0.02, 0.04, 0.08, 0.16 and 0.32 μg.mL-1. Results: The final results confirmed the significant effect of all mango and blueberry extracts concentrations on the reduction of parasite numbers (P-value<0.05(. The extract concentrations of 1000 μg.mL-1 had the most significant effect on T. vaginalis growth inhibition after 24 hours. The IC50 values of mango and blueberry extracts, metronidazole, and tinidazole were calculated as 118.3, 60.74, 0.042 and 0.02 μg.ml-1 respectively. Conclusion: Based on the obtained results, the different concentrations of mango and blueberry extracts have significant anti Trichomonas vaginalis activities. It is suggested carrying out further studies on suitable animal models.
Effect of Mexican medicinal plant used to treat trichomoniasis on Trichomonas vaginalis trophozoites
Journal of Ethnopharmacology, 2007
Crude methanolic extracts from 22 Mexican medicinal plants were screened for antitrichomonal activity against Trichomonas vaginalis, which is the etiological agent of trichomoniasis. Among the plants tested Carica papaya and Cocos nucifera showed the best antitrichomonal activity with IC 50 values of 5.6 and 5.8 g/ml, respectively. The extracts of Bocconia frutescens, Geranium mexicanum, and Lygodium venustum showed moderate activity with IC 50 values ranging from 30.9 to 60.9 g/ml. All the other plant extracts were inactive (IC 50 > 100 g/ml). All extracts tested were less active than metronidazole (IC 50 0.037 g/ml), an antiprotozoal drug used as positive control. The results of the antiprotozoal screening support the popular uses of five of the plants tested for the treatment of some urogenital tract disorders in Mexican traditional medicine. However, seeds of Carica papaya and aerial parts of Bocconia frutescens should be used in herbal medicine with care to avoid toxicity.
Assessment of the Impact of Numerous Medicinal Plants on Trichomonas Vaginalis: A Review
Indian Scientific Journal Of Research In Engineering And Management, 2024
The most common non-viral sexually transmitted illness in the world, trichomoniasis, is caused by the etiological agent Trichomonas vaginalis. Trichomoniasis is a prevalent, global health issue that is becoming worse. While infections in males are typically asymptomatic, infections in the female genital tract can result in a variety of symptoms, including vaginitis and cervicitis. This condition has historically been underdiagnosed and under researched due to its generally mild symptoms and the absence of any evidence for any major repercussions. The attempts to identify and treat patients harbouring this parasite have risen, however, in light of mounting evidence that T. vaginalis infection is linked to various disease states with high morbidity in both men and women. Recent research has highlighted the complex interactions between the parasite and host, commensal microbiota, and associated symbionts. The pathophysiology of trichomoniasis is produced by damage to the host epithelia, mediated by a multitude of events during infection. The number of accessible diagnostic alternatives has increased as a result of the commercial introduction of several nucleic acid amplification tests (NAATs). Immunoassay based Point of Care testing is currently available, and a recent initial evaluation of a NAAT Point of Care system has given promising results, which would enable testing and treatment in a single visit.
Journal of Parasitic Diseases, 2013
Trichomonas vaginalis is a protozoan parasite that causes trichomoniasis; a cosmopolitan sexually transmitted disease. Metronidazole is the drug of choice for T. vaginalis infections. The increase in metronidazole resistant parasites and undesirable side effects of this drug makes the search for an alternative a priority for the management of trichomoniasis. Pistacia lentiscus mastic and Ocimum basilicum oil are known for their antibacterial, antifungal, antiviral and antiprotozoal effects. The present study was carried out to investigate the in vitro effects of P. lentiscus mastic and O. basilicum oil on T. vaginalis trophozoites. The effects of different concentrations of P. lentiscus mastic (15, 10 and 5 mg/ml) and different concentrations of O. basilicum oil (30, 20 and 10 lg/ml) on multiplication of trophozoites at different time points (after 24, 48, 72 and 96 h) were determined, also morphological changes were reported by transmission electron microscopy (TEM). The results showed that both plants caused an inhibition of growth of T. vaginalis trophozoites. The minimal lethal concentration of P. lentiscus mastic was 15 mg/ml after 24 h incubation, 10 mg/ml after 48 h and 5 mg/ml after 96 h. The minimal lethal concentration of O. basilicum oil was 30 lg/ml after 24 h incubation, 20 lg/ml after 48 h and 10 lg/ml after 96 h. TEM study of trophozoites treated by P. lentiscus mastic or by O. basilicum oil showed considerable damage of the membrane system of the trophozoites, and extensive vacuolization of the cytoplasm. These results highly suggest that P. lentiscus mastic and O. basilicum oil may be promising phytotherapeutic agents for trichomoniasis treatment.
Journal of Obstetrics, Gynecology and Cancer Research | ISSN, 2020
Trichomonas vaginitis is the most common non-viral disease that is transmitted sexually. Although metronidazole is the most effective medication for this disease, it has some adverse effects. Herbal medicines have fewer side effects and are more popular among people. This study aimed to review the plants, which are effective against Trichomonas vaginalis and their therapeutic effects. For this purpose, the databases of Scopus, PubMed, Medline, Google Scholar, SID, and Magiran were searched for the articles published during 2000-2019 by keywords, such as Trichomonas vaginalis, complimentary, complementary therapies, herbal medicine, medicinal plants, and their Persian equivalents. All in vivo and in vitro trial studies were included in the present review. The data indicated that some compounds, including eucalyptus, case, and garlic were effective in inhibiting the growth of T. vaginalis.
BioMed research international, 2013
Trichomonas vaginalis, a flagellate protozoan, is the causative agent of trichomonosis, the most common nonviral sexually transmitted disease worldwide. Taking into account the increased prevalence of metronidazole-resistant isolates, alternative drugs are essential for the successful treatment. Natural products are the source of most new drugs, and popular wisdom about the use of medicinal plants is a powerful tool in this search. In this study, the activity of 10 medicinal plants extensively used in daily life by Mbyá-Guarani indigenous group was evaluated against seven different T. vaginalis isolates. Among the aqueous extracts tested, Verbena sp. (Guachu ka'a in Mbyá-Guarani language) and Campomanesia xanthocarpa (Guavira in Mbyá-Guarani language) showed the highest activity against T. vaginalis with MIC value of 4.0 mg/mL reaching 100% of efficacy against the parasite. The kinetic growth assays showed that the extracts promoted complete growth abolishment after 4 h of incub...
Medicinal Plants with Anti-Trichomonas vaginalis Activity in Iran: A Systematic Review
Iranian Journal of Parasitology
Background: Trichomoniasis, due to Trichomonas vaginalis, is one of the most common sexually transmitted parasitic diseases in the world such as Iran. This systematic review aimed to explore the studies evaluating the medicinal herbs with anti- T. vaginalis activity which used in Iran. Methods: Articles published in 4 Persian and 4 English databases were obtained between 2000 and 2015 including Google Scholar, PubMed, Science Direct, Scopus, Magiran, Barakatkns (formerly IranMedex), Elm net, and SID (Scientific Information Database). Studies out of Iran, studies on animal models and articles on other parasite species than T. vaginalis were excluded from this review. Results: Twenty-one articles including in vitro experiments, met our eligibility criteria. Thoroughly, 26 types of plants were examined against T. vaginalis. Medicinal herbs such as Artemisia, Zataria multiflora, and Lavandula angustifolia are remarkably effective on T. vaginalis. As such, use of other parts of these pla...
Inhibitory effects of Thai plants β-glycosides on Trichomonas vaginalis
Parasitology Research, 2008
Trichomoniasis is now an important health problem in developing countries. Although metronidazole has so far been widely used to treat this disease, the prevalence of metronidazole-resistant protozoa and unpleasant adverse effects have been found. In this study, natural products purified from Thai plants were, therefore, investigated for their effectiveness against Trichomonas vaginalis. The minimal inhibitory concentrations for all β-glycosides against Trichomonas vaginalis at 24 h were in a range of 6.25-12.5 μM. In addition, torvoside A and H were found to be more potent than their corresponding aglycones, deglucosylated torvoside A and H, while other β-glycosides were generally as active as their corresponding aglycones. The cytotoxicity of these compounds was also determined. Except for dalcochinin, none of the tested compounds showed cytotoxicity against Vero and cancer cell lines (KB and MCF-7), having IC 50 values greater than 50 μg/ml. In conclusion, β-glycosides and several aglycones showed selective inhibition against Trichomonas vaginalis without harmful effect to mammalian cells.