Photoprotection by honeybush extracts, hesperidin and mangiferin against UVB-induced skin damage in SKH-1 mice (original) (raw)
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Antioxidants
In the present study, we aimed to examine the antioxidant, antiaging and photoprotective properties of Greek honey samples of various botanical and geographical origin. Ethyl-acetate extracts were used and the and the total phenolic/flavonoid content and antioxidant capacity were evaluated. Honey extracts were then studied for their cytoprotective properties against UVB-induced photodamage using human immortalized keratinocytes (HaCaT) and/or reconstituted human skin tissue models. Specifically, the cytotoxicity, oxidative status, DNA damage and gene expression levels of specific matrix metalloproteinases (MMPs) were examined. Overall, the treatment of HaCaT cells with honey extracts resulted in lower levels of DNA strand breaks and attenuated the decrease in cell viability following UVB exposure. Additionally, honey extracts significantly decreased the total protein carbonyl content of the irradiated cells, however, they had no significant effect on their total antioxidant status. ...
Biotechnology and Bioprocess Engineering, 2012
Sea buckthorn (Hippophae rhamnoides L.) seed, which has very high nutraceutical, cosmetoceutical and therapeutic properties, has been widely used to treat diseases in Tibetan and Mongolian traditional medicines. In this study, we measured the antioxidant activities of the solvent-dependent SBS (Sea buckthorn seed) extracts using the DPPH and Folin-Ciocalteu assays and we investigated the efficacy of SBS in protecting skin against UVB-induced damage using cultured human dermal fibroblasts. In addition, the inhibitory effects of SBS on UVB-induced skin photoaging were examined by determining the level of Metalloproteinase (MMP)-1 and procollagen after UVB-irradiation. The antioxidant capacity of the SBSE (alcoholic) extract was significantly higher than the SBSH (hexane) and SBSW (water) extracts, as measured based on the free-radical scavenging activity and total phenolic content. SBSE was chosen as the most suitable candidate antioxidant. In human dermal fibroblasts, the cell viability of SBSE extract at doses of 2.5, 5, and 10 µg/mL was higher than the UVB-treated control. By RT-PCR and Western blot, SBSE treatment inhibited UVBinduced IL-1β expression in cultured cells. In addition, SBSE restrained UVB-induced IL-6 and COX-2 gene expression in cultured fibroblasts in a dose-dependent manner. Treatment with the SBSE extract after UVB irradiation in human dermal fibroblasts significantly reduced MMP-1 expression and increased procollagen synthesis when compared with UVB-irradiation only. In this study, SBSE was shown to increase the synthesis of procollagen, decrease the expression of MMP-1, and inhibit the production of IL-1β, IL-6, and COX-2 in UVB-irradiated human fibroblasts. These findings suggest that the SBSE extract may be a potential therapeutic agent for preventing and treating skin photoaging.
Protection from UVB Toxicity in Human Keratinocytes by Thailand Native Herbs Extracts
Photochemistry and Photobiology, 2014
Thai traditional medicine employs a wide range of indigenous herbs in the forms of tincture or tea for the cure of skin and systemic inflammatory diseases. The protection by Thai plants extracts against UVB DNA damage and cytotoxicity was investigated in human keratinocytes. Petroleum ether, dichloromethane and ethanol extracts were prepared from 15 Thai herb species, and the total phenolic and flavonoid contents, the antioxidant and UV-absorbing properties were assessed by standard procedures. Cytoprotective effects were evaluated on the basis of cell survival, caspase-3 activity and pyrimidine dimers determination. High total phenolic and flavonoid contents were found in the ethanol and dichloromethane fractions. Dichloromethane extract of turmeric was shown to possess the highest antioxidant activity. The maximum UV absorptions were found in the ethanol extract of turmeric and in the dichloromethane extract of ginger. These extracts stimulated the synthesis of Thioredoxin 1, an antioxidant protein, and could protect human HaCaT keratinocytes from UV-induced DNA damage and cytotoxicity. The present data support the utilization of turmeric and ginger extracts in anti-UV cosmetic pharmaceuticals.
The Health Benefits of Natural Skin Uva Photoprotective Compounds Found in Botanical Sources
International Journal of Pharmacy and Pharmaceutical Sciences, 2016
In recent years, botanicals have gained importance due to their dermal photoprotective effect against the harmful UV radiation. This radiation generates reactive oxygen species which attack proteins, lipids, and nucleic acids among others. This leads to the cellular oxidation, which is reflected as tanning, skin inflammation, erythema, immunosuppression, photoaging and skin cancer. The photoprotective activity of synthetic sunscreens is unsatisfactory due the proved incomplete spectral protection, toxicity, and allergenicity. The phytotherapy treatment with antioxidant compounds with potential UV absorbing capacity could offer a more effective photoprotection since they may impede, reverse or delay the process of formation of DNA adducts. The wide diversity of botanicals with photoprotective activity is attributed to several types of substances with phenolic chromophores. These compounds can be classified as flavonoids, phytoestrogens, carotenoids, xhantophylls, coumarins, proanthoc...
Antioxidants and Skin Protection
Antioxidants, 2020
Natural products have a long history of use for skincare and the improvement of the appearance and function of aged and/or damaged skin. Among them, bioactive peptides, oligosaccharides, plant polyphenols, carotenoids, vitamins, and polyunsaturated fatty acids are the most widely used ingredients. In recent decades, natural products have undergone rigorous testing, resulting in the identification of phytochemical compounds such as antioxidants with important potentials for the development of cosmetics, cosmeceuticals, and nutraceuticals. Supplementation with these products has been shown to have an effect on the signs of ageing in several human trials. In this Special Issue, up to 12 original manuscripts and a review are included, covering most of the topics related to the use of natural compounds for skincare and protection. This editorial aims to highlight the most relevant contributions of each single manuscript to the current "state of the art" and provide global significance for the use of antioxidants in skincare. Agulló-Chazarra and co-workers [1] have investigated the use of an agriculture by-product to obtain potential ingredients for the cosmetic industry. They have used sweet cherry stems, a novel by-product that has hardly been explored until now. In addition, they have developed an interesting approach, using a multistep procedure covering several aspects of antioxidant and related bioactive properties to select the best extract and propose it as an interesting ingredient for the cosmetic industry. Benincasa et al. [2] have used another raw material to develop their study; in this case, they have studied the use of olive oil by-products to obtain a pure compound, hydroxytyrosyl oleate (HtyOle), and then study its antioxidant and regenerative properties on human keratinocytes. The use of by-products from agriculture is really interesting, because it aligns with the Sustainable Development Goals of the United Nations for 2030 [3] and promotes the use and valorisation of non-previously or low-used materials for high-value applications. The photoprotective properties of natural products has been addressed by five of the original articles of this Special Issue as their central topic [4-8]. These articles show up the relevance of photoprotection not only in cosmetics but also in cancer research, as UV radiation is the main cause of some melanoma subtypes. In the first of these articles, Kuo et al. [4] focused on the role of N-(4-bromophenethyl)-caffeamide (K36H), a propolis derivative, as a protective compound against UVA-induced apoptosis. This compound has showed its antioxidant activity by reducing reactive oxygen species (ROS) generation and decreasing metalloproteinase expression, DNA damage, and inflammation. The authors has also identified the main molecular mediators of all these actions and provided a global mechanism of how K36H acts inside human keratinocyte cells. A similar approach has been used by Wu et al. in their contribution to this Special Issue [8], testing the photoprotective and anti-inflammatory properties of a sesamin derivative on human fibroblasts. Tavares and colleagues has used mouse fibroblasts, human keratinocytes, and full-thickness reconstructed human skin to demonstrate the photoprotective actions of fucoxanthin [7]. Their results show that this compound is able to reduce ROS even when used in a prototype of final formulation
Plant extracts and natural compounds used against UVB-induced photoaging
Biogerontology, 2017
Skin is continuously exposed to a variety of environmental stresses, including ultraviolet (UV) radiation. UVB is an inherent component of sunlight that crosses the epidermis and reaches the upper dermis, leading to increased oxidative stress, activation of inflammatory response and accumulation of DNA damage among other effects. The increase in UVB radiation on earth due to the destruction of stratospheric ozone poses a major environmental threat to the skin, increasing the risk of damage with long-term consequences, such as photoaging and photocarcinogenesis. Extracts from plants and natural compounds have been historically used in traditional medicine in the form of teas and ointments but the effect of most of these compounds has yet to be verified. Regarding the increasing concern of the population with issues related to quality of life and appearance, the cosmetic market for anti-aging and photoprotective products based on natural compounds is continuously growing, and there is increasing requirement of expansion on research in this field. In this review we summarized the most current and relevant information concerning plant extracts and natural compounds that are able to protect or mitigate the deleterious effects caused by photoaging in different experimental models.
Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie, 2018
This study analyzed fruit stem extract (MGFE) from Muscat Bailey A grape (Vitis labrusca × Vitis vinifera) for their ameliorative effects on Ultraviolet B (UVB)-induced skin damage in Balb/c mice. Well established in vivo assays were used to determine the biological effects of MGFE upon UVB irradiation of BALB/c mice. The results showed that treatment with MGFE recovered glutathione depletion, prevented lipid peroxidation of tissues and decreased the expression of DNA repair enzyme oxo guanine glycosylase-1. MGFE recovered the skin conditions in UVB-irradiated Balb/c mice. Moreover, MGFE inhibited dermal infiltration of inflammatory cells and reduced serum tumor necrosis factor alpha and interleukin-6 levels. Finally, MGFE treatment inhibited UVB-induced melanin formation and collagen fiber destruction through the inhibition of matrix metalloproteinase-1 expression. Through high-performance liquid chromatography analysis, catechin, epicatechin, and trans-resveratrol were found to be...
Frontiers in Pharmacology, 2021
Polyherbal formulation combining multiple herbs is suggested to achieve enhanced therapeutic effects and reduce toxicity. Harak herbal formula (HRF) extracts were proposed to regulate skin responses to UVR through their ability to suppress UVA-induced matrix metalloproteinase-1 (MMP-1) and pigmentation via promoting antioxidant defenses in in vitro models. Therefore, natural products targeting Nrf2 (nuclear factor erythroid 2-related factor 2)-regulated antioxidant response might represent promising anti-photoaging candidates. Hesperetin (HSP) was suggested as a putative bioactive compound of the HRF, as previously shown by its chemical profiling using the liquid chromatography coupled with quadrupole time-of-flight mass spectrometry (LC-QTOF-MS). In this study, we explored the anti-photoaging effects of HRF extracts and HSP on normal human dermal fibroblasts (NHDFs) and mouse skin exposed to UVA irradiation. Pretreatment of NHDFs with HRF extracts and HSP protected against UVA (8 J...
2016
In recent years, botanicals have gained importance due to their dermal photoprotective effect against the harmful UV radiation. This radiation generates reactive oxygen species which attack proteins, lipids, and nucleic acids among others. This leads to the cellular oxidation, which is reflected as tanning, skin inflammation, erythema, immunosuppression, photoaging and skin cancer. The photoprotective activity of synthetic sunscreens is unsatisfactory due the proved incomplete spectral protection, toxicity, and allergenicity. The phytotherapy treatment with antioxidant compounds with potential UV absorbing capacity could offer a more effective photoprotection since they may impede, reverse or delay the process of formation of DNA adducts. The wide diversity of botanicals with photoprotective activity is attributed to several types of substances with phenolic chromophores. These compounds can be classified as flavonoids, phytoestrogens, carotenoids, xhantophylls, coumarins, proanthocyanidins, anthocyanins, catechins, phenolic acids, triterpenes, alkaloids, etc. The bioactivity of these compounds is not limited to the photoprotective action, but antioxidant, anti-inflammatory, antimutagenic and immunomodulatory properties. The regular intake of these botanicals not only prevents but also delays the deleterious effects of sun radiation onto the skin. Therefore, these botanicals have gained considerable attention and are now included in most vitamin and cosmetic products. This review gives an overview of photoprotective botanicals mainly focused on UVA chromophores that are able to inhibit or reverse the damaging effects of sun radiation. The search criterion was made essentially on UVA photoprotective botanicals and related works published in the last ten years. The literature search included mainly the Google scholar, Science direct, and ISI web of knowledge databases.
Photoprotective potential in some medicinal plants used to treat skin diseases in Sri Lanka
BMC Complementary and Alternative Medicine, 2016
Background: The constant exposure to solar ultraviolet radiation (UV) has a variety of harmful effects on human health. Although synthetic sunscreen products have been introduced as a preventive/therapeutic strategy, with the realization of their adverse side effects, the recent trend is to search for human friendly alternative formulations especially of plant origin. Therefore, the present study focuses on evaluation of photoprotective activity of aqueous extracts (1 mg/ml) of eleven medicinal plants in Sri Lanka that have been widely employed in traditional medicine as treatment options for various skin diseases and to improve the complexion. Methods: For the determination of UV filtering potential of the extracts, UV absorption was measured and the sun protection factor (SPF) was calculated according the Mansur equation. The antioxidant activity was evaluated by DPPH and ABTS assays.