Newly Isolated Ligninolytic Bacteria and Its Applications for Multiple Dye Degradation (original) (raw)
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Advances in Bioresearch, 2020
Today with growing textile industry, the major problem which India is facing is to remove dye from effluent before discharge in the water bodies. In the present study, Lignin peroxidase (Lip) enzyme producing microbial strain FSV 3was studied for decolorization and detoxification of various synthetic dye like Congo red, Methyl orange, Methylene blue and Remazol brillent blue R. From the study, it was found that FSV 3 strain is able to decolorize and detoxify methylene blue and Remazol brillent blue R upto 100mg/L by 44 and 77% respectively on 144 hours of study, while for congo red and methylene orange show bioabsorption of dye.
Biodegradation of synthetic textile dyes by thermophilic lignolytic fungal isolates
Journal of Advanced Laboratory Research in Biology, 2014
Synthetic dyes are extensively used in different industries like textile dyeing, paper, printing, color, photography, pharmaceutics and cosmetics. These are generally toxic and carcinogenic in nature. If not treated, they will remain in nature for a long period of time as they are recalcitrant. Among these, azo dyes represent the largest and most versatile class of synthetic dyes. Approximately 10-15% of the dyes are released into the environment during manufacture and usage. Various methods are used for dye removal viz. physical, chemical, electrochemical and biological. Advantage of chemical, electrochemical and biological methods over physical involves the complete destruction of the dye, but chemical and electrochemical methods are found to be expensive and have operational problems. So the biological method is preferred over other methods for degradation/decolorization of dyes. In the present study, thermophilic lignolytic fungal culture was isolated from compost/soil/digested slurry/plant debris, were subjected for acclimatization to Remazol Brilliant Blue (RBB) at 0.05% concentration, in the malt extract broth (MEB). The most promising fungal isolates were used for further dye degradation studies. The results suggest that the isolates T10, T14 and T17 as a useful tool for degradation of reactive dyes.
Isolation and screening of bacterial isolates for degradation of recalcitrant industrial waste dyes
International Journal of Chemical Studies, 2019
This study was aimed to screen recalcitrant industrial waste dyed degrading ability of Bacteria isolated from the soil and sludge samples collected from Yavatmal region of Maharashtra State of India. The strains were isolated for their ability to degrade dyes like Congo red, Indigo blue dyes, which are difficult to degrade, hazardous, and carcinogenic. The Bacterial isolates are suppose to produce enzymes like, Peroxidase, and Laccase which play important role in degradation of recalcitrant dyes by oxidation. Further the isolates were characterised based on their utilization of sugar. In this study the Bacterial isolates RS1and BH1 which have good dye degradation ability was successfully isolated and characterized.
Contribution of lignin degrading enzymes in Decolourisation & degradation of reactive textile dyes
Investigating the potential of Phanerochaete chrysosporium to decolourise and degrade two reactive textile dyes (Reactive Yellow MERL and Reactive Red ME4BL) was the main intend of the study. The fungus analysed for its decolourising potential has shown the significant success by removing the colour of tested dyes (10 mg/L concentration) within 11 days of incubation. Supplementing media with different carbon/nitrogen sources proved dextrose and aspargine as efficient decolorizing enhancers, while inoculum size of 1 and 3 (10 mm diameter agar plug) were more supportive for solid and liquid decolourisation respectively. The ligninolytic enzyme production under solid state fermentation involved different agro-industrial wastes, among which wheat straw with 1 mm particle size was responsible for optimum production of manganese peroxidase (607.35 IU/ml), manganese independent peroxidase (539.27 IU/ml) and laccase (263.03 IU/ml). The partially purified enzymes produced by P. chrysosporium was achieved at four different percent saturations i.e. 20, 40, 60 and 80, where 60% saturated fraction produced the maximum 607.35 IU/ml of manganese peroxidase having 52.8 kDa molecular weight. The biodegradation of Reactive Yellow MERL (7-(4-{4-chloro-6-[3-(2-sulfoxy-ethanesulfonyl)-phenylamino]-[1,3,5]triazin-2-ylamino}-2-ureido-phenylazo)-naphthalene-1,3,6-trisulfonic acid) and Reactive Red ME4BL (5-{4-choloro-6-[4-(2-sulfo-ehtanesulfonyl)-phenylamino]-[1,3,5]triazin-2-ylamino}-3-(1,5-disulfo-napthalen-2-ylazo)-4-hydroxy-naphthalena-2,7-disulfonic acid) were studied by FTIR analysis where shifting of peaks confirmed the complete degradation of both the dyes.
Soil enriched with dye from Textile industry (Baddi) Dupatta house, Nalagarh, Dupatta staining house, Baddi Textile industry, kharuni, Nalagarh was taken. Isolation of bacteria from different soil samples was done. Morphological Characterization of isolates was done with the help of Gram Staining. Biochemical characterization of isolates was reported with the help of Indole, Methyl red, Catalase, Nitrogen reductase & Urease, Citrate Hydrolysis, Hydrogen sulfide test. ? ?max of methyl orange dye was determined. Optimization of the decolonization condition was done by optimizing its pH & temperature as well as rate of Decolourization of methyl orange dye was determined. Screening of the most efficient bacterial strain for dye decolourizaion was done with the help of Nitrate reduction test This test is based on the detection of nitrite and its ability to form red color compound when it reacts with sulfanilic acid (Reagent A) to form a complex (Nitrite-sulfanilic acid) which then reacts with ?-Naphthylamine (Reagent B) to five red precipitates (prontosil).
Bacterial biodegradation and decolourization of toxic textile azo dyes
Dyes released by the textile industries pose a threat to environmental safety. Recently, dye decolourization through biological means has gained momentum as these are cheap and can be applied to wide range of dyes. This review paper focuses on the bacterial biodegradation of toxic textile dyes. Along with the early history of dyes, the toxic effects of textile azo dyes also focused in this review. Various biological techniques for the biodegradation of textile dyes by microorganisms are clearly given in this review. The advantages of aerobic biodegradation over anaerobic degradation are also discussed in this review. The studies discussed in this paper indicate biological decolourization by bacteria has a great potential to be developed further as a decentralized wastewater treatment technology for small textile or dyeing units. However, further research work is required to study the toxicity of the metabolites of dye degradation and the possible fate of the utilized biomass in order to ensure the development of an eco-friendly technology.
Screening of ligninolytic fungi for bioremediation of dyes
Asia-pacific Journal of Molecular Biology and Biotechnology, 2021
Water pollution is a growing concern worldwide. One of the main causes of water pollution includes the textile industry which produces a large amount of wastewater every day. This wastewater is known to contain dyes that are recalcitrant and hard to treat. In order to solve this problem, bioremediation using ligninolytic fungi is commonly used for the ligninolytic enzymes which are able to break down the dyes. In this study, samples were collected from decaying woods and soils in the vicinity of UiTM Puncak Alam forests. A total of 20 fungal isolates were tested for ligninolytic enzyme production. Out of the 20 isolates, 13 were found to produce lignin peroxidase and manganese peroxidase, but only one produced laccase. The isolate that produced all three enzymes was used for DNA isolation and identified using amplification of the ITS region by PCR. The isolate was identified as Trichoderma asperellum, a soft rot fungal species which is renowned for its role in bioremediation as a bi...
Textile azo dye decolorization and detoxification using bacteria isolated from textile effluents
BioTechnologia
Azo dyes, which are highly toxic, carcinogenic, and mutagenic for living organisms, are used as coloring chemicals in textile industries. Physicochemical methods used for removing azo dyes are expensive, can generate secondary waste, and are not very efficient. In this study, we used a biological approach to reduce the toxicity of three azo dyes, i.e., congo red, methyl orange, and reactive red 198, from textile effluents. Six dye-decolorizing bacteria were screened from waste water obtained from the textile industry (in the dyeing process) at a concentration 100 mg/l for each azo dye. Using a 16S rRNA approach, the bacteria were identified and assigned as Enterococcus faecalis VTK04, Staphylococcus aureus VTK013, Pseudomonas aeruginosa VTK018, Proteus mirabilis VTK024, Bacillus cereus VTK035, and Enterococcus faecium VTK054. These bacteria were tested for their ability to produce biofilms on the abiotic surface. The adherence assay showed that VTK013, VTK054, and VTK024 had a potential to form stable biofilms on abiotic surfaces (OD570 = 1.37). Moreover, the dye decolorization potential was spectrophotometrically measured after seven days with and without a carbon substrate. The results demonstrated that the absence of the carbon source had a negative impact on decolorization, whereas a carbon-supplemented medium showed a considerable increase in the decolorization of congo red (80% and 96% by VTK013 and VTK018, respectively), methyl orange (100% and 75% by VTK054 and VTK035, respectively), and reactive red 198 (90% and 86% by VTK04 and VTK024, respectively). The phytotoxicity study of the treated dye solutions showed lesser toxicity compared to the untreated dye solution. These results support the possibility of using bacterial isolates for the biodegradation of azo dye effluents.
2019
The textile dye residues represent a growing threat to the surrounding environment of this vital industry. Therefore, the appropriate technology for the removal of dye residues from industrial effluents is an important task. One of these technologies is based on the bioremediation that depends on using certain microorganisms to remove these toxic wastes. It is well known that the microbial agents perform all biological transformations through immense enzymatic tools existing within the intact microbial cells. The use of the specific enzymes required for breakdown of certain industrial wastes can reduce the time of residues removal and consequently the cost of the process. In this study the production of two fungal enzymes namely laccases and peroxidases were assessed. To study these enzymes fifteen fungal strains were first screened for their growth on mineral salt medium supplemented with 0.5g/L of green azo dyes as sole source of carbon. The strains could grow on the above mention...