Antimicrobial activity of calcium hydroxide and chlorhexidine on intratubular Candida albicans (original) (raw)
Related papers
Effectiveness of a 2% chlorhexidine solution mixed with calcium hydroxide against Candida albicans
Australian Endodontic Journal, 2008
The purpose of this investigation was to examine the in vitro susceptibility of Candida albicans to a mixture of chlorhexidine and calcium hydroxide using a tube dilution test. A suspension containing C. albicans was exposed to 2% chlorhexidine solution, calcium hydroxide paste and 2% chlorhexidine mixed with calcium hydroxide in plastic tissue culture wells. The tested agents were incubated with C. albicans for 1, 24, or 72 h. All agents were effective antifungals at all experimental time periods tested, with the exception of exposure to calcium hydroxide for 1 h. A mixture of 2% chlorhexidine and calcium hydroxide therefore is a very effective anti-fungal agent against C. albicans.
Oral Surgery, Oral Medicine, Oral Pathology, Oral Radiology, and Endodontology, 2006
The purpose of this study was to compare the effectiveness of various medicaments, including Ca(OH) 2 / 2% chlorhexidine, 2% chlorhexidine gel, and Ca(OH) 2 alone, against Enterococcus faecalis and Candid albicans in vitro. Study design. Eighty extracted single-rooted human maxillary teeth were used. After removing the crown, each root was instrumented up to size 50 by using a conventional technique. The root canal was irrigated with ethylenediamine tetra-acetic acid (EDTA) solution to remove smear layer. Then, roots were infected with E faecalis and C albicans. Subsequently, the roots were divided into 4 treatment groups: group 1 was treated with calcium powder hydroxide in distilled water, group 2 was treated with calcium hydroxide powder in 2% chlorhexidine, group 3 was treated with 2% chlorhexidine gel, and group 4 was treated with 0.9% sterile saline serving as negative control. Microbial samples were taken after 7, 15, and 30 days. After incubation, dentine chips were obtained form each root canal and examined microbiologically. The microbiological samples were plated to count colony-forming units in per milligram of dentin. Results. The results showed that the 2% chlorhexidine gel was significantly more effective than calcium hydroxide with 2% chlorhexidine, calcium hydroxide, and control saline solution (P Ͻ .05). Conlusion. Under the conditions of this study, 2% chlorhexidine gel is effective in the elimination of E faecalis and C albicans from the root canal system. However, to support this in vitro observation, further in vivo studies are needed. (Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2006;102:e27-e31) MATERIAL AND METHODS Eighty freshly extracted human upper anterior teeth were placed in 2.5% sodium hypochlorite for 5 hours, after which any bone, calculus, or soft tissues on the root surfaces were removed with curettes.
In Vitro Study of the Antibacterial Activity of Calcium Hydroxide on Candida Albicans
2014
The aim of this in vitro study is to evaluate the antimicrobial activity of calcium hydroxide used in eliminating Candida albicans. In the present study we used clinical isolates of Candida albicans from primary infected root canals with opened pulp chamber and acute apical periodontitis. An agar-diffusion method was used to investigate the antimicrobial activity of Calcium hydroxide against Candida albicans. Calcium hydroxide was used mixed with different additives: Calcium hydroxide and destilated water (mixed ex tempore) Calcium hydroxide and 2% Chlorhexidine gluconate (mixed ex tempore), Calcium hydroxide and Iodoform (Metapex paste). In each plate three cavities were filled with one of the calcium pastes and one cavity- control agent. The zones of inhibition around the used medicaments in the cavities were measured in millimeters after being incubated in the period at 37°C for the period of 24 - 48 hours. The data was input and processed using the statistical package SPSS 15.0....
Dentistry, 2013
Objective: The aim of this study was to evaluate the effects of calcium hydroxide, chlorhexidine, and mineral trioxide aggregate (MTA) on the viability of Candida albicans. Method: Sabouraud Dextrose Agar plates were prepared that contained different concentrations of calcium hydroxide, chlorhexidine, or MTA powder. The plates were inoculated with an overnight culture of C. albicans, and the presence of colonies that formed were observed after incubation at 37°C for 1, 24, 48, and 72 hours. Results: Chlorhexidine and MTA, but not calcium hydroxide, inhibited colony formation. The minimum inhibitory concentration of MTA and chlorhexidine against C. albicans was 50 mg/ml. Conclusions: We found that MTA and chlorhexidine inhibited the growth in agar of C. albicans within three days.
Antimicrobial Effects of Calcium Hydroxide and Chlorhexidine on Enterococcus faecalis
Journal of Endodontics, 2010
Introduction: Endodontic treatment is commonly based on nonspecific elimination of intraradicular microorganisms. Although some authors prefer single-visit root canal operations for endodontic treatment, several studies have shown the importance of intracanal medication between sessions to kill microorganisms that biomechanical preparations alone cannot achieve. The purpose of this study was to evaluate the efficacy of calcium hydroxide Ca(OH) 2 and chlorhexidine gel on the elimination of intratubular Enterococcus faecalis. Methods: Human uniradicular teeth contaminated with E. faecalis were treated with Ca(OH) 2 , 2% chlorhexidine gel, Ca(OH) 2 plus 2% chlorhexidine gel, or saline (0.9% NaCl) as a negative control. Samples obtained at a depth of 0 to 100 mm and 100 to 200 mm from these root canal preparations were analyzed for bacterial load by counting the number of colonyforming units (CFUs) and bacterial viability using fluorescence microscopy. Results: A significant decrease in the number of CFUs and the percentage of viable E. faecalis was observed after treatment with either Ca(OH) 2 or chlorhexidine when compared with the control group. Additionally, chlorhexidine gel had a significantly higher antimicrobial efficacy as measured by the number of CFUs and the percentage of viable cells than Ca(OH) 2 . No differences were observed between the antimicrobial properties of chlorhexidine gel with and without the addition of Ca(OH) 2 . Conclusion: Both Ca(OH) 2 and chlorhexidine have antimicrobial effects on E. faecalis. Chlorhexidine had increased antimicrobial activity when compared with Ca(OH) 2. Ca(OH) 2 combined with chlorhexidine showed similar antimicrobial activity to chlorhexidine alone. (J Endod 2010;36:1389-1393
Dental Journal (Majalah Kedokteran Gigi), 2006
Calcium Hydroxide point and Chlorhexidine point are new drugs for eliminating bacteria in the root canal. The points slowly and controly realease Calcium Hydroxide and Chlorhexidine into root canal. The purpose of the study was to determined the effectivity of Calcium hydroxide point (Calcium hydroxide plus point) and Chlorhexidine point in eleminating the root canal bacteria of nescrosis teeth. In this study 14 subjects were divided into 2 groups. The first group was treated with Calcium hydroxide point and the second was treated with Chlorhexidine poin. The bacteriological sampling were measured with spectrofotometry. The Paired T Test analysis (before and after) showed significant difference between the first and second group. The Independent T Test which analysed the effectivity of both groups had not showed significant difference. Although there was no significant difference in statistical test, the result of second group eliminate more bacteria than the first group. The present finding indicated that the use of Chlorhexidine point was better than Calcium hydroxide point in seven days period. The conclusion is Chlorhexidine point and Calcium hydroxide point as root canal medicament effectively eliminate root canal bacteria of necrosis teeth.
Elimination of Candida albicans Infection of the Radicular Dentin by Intracanal Medications
Journal of Endodontics, 2003
Fungi have been associated with cases of secondary or persistent root canal infections. The purpose of this study was to evaluate the effectiveness of four intracanal medications in disinfecting the root dentin of bovine teeth experimentally infected with Candida albicans. Infected dentin cylinders were exposed to four different medications: calcium hydroxide/glycerin; calcium hydroxide/0.12% chlorhexidine digluconate; calcium hydroxide/camphorated paramonochlorophenol/glycerin; and 0.12% chlorhexidine digluconate/zinc oxide. Specimens were in contact with the medications for 1 h, 2 days, and 7 days. The viability of C. albicans after exposure was evaluated by specimen incubation in culture medium to compare the effectiveness of the medications in disinfecting dentin. Results showed that the specimens treated with calcium hydroxide/camphorated paramonochlorophenol/ glycerin paste or with chlorhexidine/zinc oxide paste were completely disinfected after 1 h of exposure. Calcium hydroxide/glycerin paste only consistently eliminated C. albicans infection after 7 days of exposure. Calcium hydroxide mixed with chlorhexidine was ineffective in disinfecting dentin even after 1 week of medication exposure. Among the medications tested, the calcium hydroxide/ camphorated paramonochlorophenol/glycerin paste and chlorhexidine digluconate mixed with zinc oxide were the most effective in eliminating C. albicans cells from dentinal specimens.
2017
Objective: Candida albicans,is one of the most resistant pathogens found in root canals with necrotic pulp and in recurrent root canal failure cases. Removal of this resistant microorganisms from the root canal poses a special challenge by routine instruments and conventional irrigants. The objective of the study is to compare the anti-fungal efficacy of Fluconazole with two most commonly used root canal irrigating solution (3% NaOCl) Sodium hypochlorite and 17% EDTA through specific methodology that can project Fluconazole as an antifungal irrigant of choice. Method: Twenty-five extracted human maxillary central incisors were used in this study with biomechanical preparation done to Protaper F3. Teeth were randomly divided into three test groups (n=5) and a positive control group. After cleaning and shaping, root canals were contaminated with C. albicans and incubated for 72 hours. Then the root canals were irrigated with the antifungal solutions and enlarged to Protaper F4. Genera...