Ultrasonic agitation reduces the time of calcium hydroxide antimicrobial effect and enhances its penetrability (original) (raw)
Related papers
Journal of Applied Oral Science, 2016
O bjective: The antimicrobial effect of ultrasonic agitation of calcium hydroxide (CH) pastes in infected bovine dentin and their penetrability were evaluated using confocal laser scanning microscopy (CLSM) and microbiological culture. Material and Methods: Fifty-two bovine teeth were infected with Enterococcus faecalis using a new contamination protocol; then they received CH paste and were divided into groups with or without ultrasound. Ultrasonic agitation was conducted for 1 min with a plain point insert. After 15 d, the CLSM analyzed the viable and dead bacteria with Live and Dead assay. The dentinal wall debris was collected by burs, and the colony forming units (CFU/mL) were counted. The penetrability of the paste inside dentinal tubules was tested using the B-rodamine dye. Results: The calcium hydroxide paste showed better results with the use of ultrasonic agitation (p<0.05). Conclusion: The ultrasonic agitation of CH paste increased its antimicrobial action and was responsible for intradentinal penetration
Effect of Ultrasonics on Enterococcus faecalis Biofilm in a Bovine Tooth Model
Journal of Endodontics, 2011
Introduction: The purpose of this study was to evaluate in vitro the effect of the ultrasonic irrigation of sodium hypochlorite and EDTA in root canals of bovine teeth infected with Enterococcus faecalis. Methods: Eighty-four bovine incisors were inoculated with E. faecalis, remaining in culture for 50 days for biofilm formation. The teeth were divided into four groups: the control group, which received no treatment; the ultrasonic + distilled water group; the conventional irrigation with sodium hypochlorite + EDTA group; and the passive ultrasonic irrigation with sodium hypochlorite + EDTA group. Microbiological tests and analysis in scanning electron microscopy (SEM) were performed. Results: In microbiological testing, groups using sodium hypochlorite did not show bacterial growth. There were significant differences between the control group and the ultrasonic + distilled water group and between these groups and groups using sodium hypochlorite. In SEM analysis, at the canal wall area, there was no significant difference between the groups using sodium hypochlorite, but these were different from the others groups. The control group was significantly different from the ultrasonic + distilled water group. At the exposed tubule area, there was no significant difference between the groups. Conclusions: Passive ultrasonic irrigation can be an aid in cleaning the root canal; however, the main role in bacteria elimination is played by the irrigant.
Journal of Investigative and Clinical Dentistry, 2019
Endodontic treatment procedures should completely eliminate the microorganisms of the root canal; however, this is almost impossible to achieve because of the anatomic complexity. Consequently, the improvement of irrigating protocols is essential to achieve a better disinfection and greater residual antimicrobial activity in the root canals. 1 Sodium hypochlorite (NaOCl) has been the most commonly used irrigant in endodontics. 2 Although it is capable to eliminate Enterococcus faecalis (E faecalis) biofilms on dentin, 3 its high surface tension prevents the penetration in each anatomical complexity. 4 Moreover, NaOCl does not exert any residual antimicrobial activity. 5,6 The application of a final irrigating solution that remains active not only at the time of application but over a fairly long period of
Journal of Applied Oral Science, 2011
www.scielo.br/jaos A dequate denture hygiene can prevent and treat infection in edentulous patients. 7KH\ DUH XVXDOO\ HOGHUO\ DQG KDYH GLI¿FXOW\ IRU EUXVKLQJ WKHLU WHHWK 2EMHFWLYH 7KLV VWXG\ HYDOXDWHG WKH HI¿FDF\ RI FRPSOHWH GHQWXUH ELR¿OP UHPRYDO XVLQJ FKHPLFDO DONDOLQH peroxide-effervescent tablets), mechanical (ultrasonic) and combined (association of the effervescent and ultrasonic) methods. Material and Methods: Eighty complete denture ZHDUHUV SDUWLFLSDWHG LQ WKH H[SHULPHQW IRU GD\V 7KH\ ZHUH GLVWULEXWHG LQWR JURXSV Q %UXVKLQJ ZLWK ZDWHU &RQWURO (IIHUYHVFHQW WDEOHWV &RUHJD 7DEV Ultrasonic device (Ultrasonic Cleaner, model 2840 D); (4) Association of effervescent tablets DQG XOWUDVRQLF GHYLFH OOJURXSVEUXVKHGWKHLUGHQWXUHVZLWKDVSHFL¿FEUXVKOO JURXSV EUXVKHG WKHLU GHQWXUHV ZLWK D VSHFL¿F EUXVK %LWXIR DQG ZDWHU WLPHV D GD\ EHIRUH DSSO\LQJ WKHLU WUHDWPHQWV 'HQWXUH ELR¿OP ZDV FROOHFWHG DW EDVHOLQH DQG DIWHU GD\V 7R TXDQWLI\ WKH ELR¿OP WKH LQWHUQDO VXUIDFHV RI WKH PD[LOODU\ FRPSOHWH GHQWXUHV ZHUH VWDLQHG DQG SKRWRJUDSKHG DW 7KH SKRWRJUDSKV ZHUH SURFHVVHG DQG WKH DUHDV WRWDO LQWHUQDO VXUIDFH VWDLQHG ZLWK ELR¿OP TXDQWL¿HG ,PDJH 7RRO 7KH SHUFHQWDJH RI WKH ELR¿OP ZDV FDOFXODWHG E\ WKH UDWLR EHWZHHQ WKH ELR¿OP DUHD PXOWLSOLHG by 100 and the total area of the internal surface of the maxillary complete denture. 5HVXOWV 7KH .UXVNDO:DOOLV WHVW ZDV XVHG IRU FRPSDULVRQ DPRQJ JURXSV IROORZHG E\ WKH 'XQQ PXOWLSOHFRPSDULVRQ WHVW OOJURXSVEUXVKHGWKHLUGHQWXUHVZLWKDVSHFL¿FEUXVKOO WHVWV ZHUH SHUIRUPHG UHVSHFWLQJ D VLJQL¿FDQFH OHYHO RI 6LJQL¿FDQW GLIIHUHQFH ZDV IRXQG DPRQJ WKH WUHDWPHQWV .: 3 WKH PHDQ UDQNV IRU WKH WUHDWPHQWV DQG UHVXOWV IRU 'XQQ PXOWLSOH FRPSDULVRQ WHVW ZHUH Control (60.9); Chemical (37.2); Mechanical (35.2) and Combined (29.1). Conclusion: The H[SHULPHQWDO PHWKRGV ZHUH HTXDOO\ HIIHFWLYH UHJDUGLQJ WKH DELOLW\ WR UHPRYH ELR¿OP DQG ZHUH VXSHULRU WR WKH FRQWURO PHWKRG EUXVKLQJ ZLWK ZDWHU ,PPHUVLRQ LQ DONDOLQH SHUR[LGH and ultrasonic vibration can be used as auxiliary agents for cleaning complete dentures. VLQFH DQ LPSRUWDQW OLPLWDWLRQ RI WKLV VWXG\ ZDV WKDW WKH SHUR[LGHV ZHUH QRW XVHG DV FRQGXFWLQJ ÀXLG LQ the ultrasonic device. CONCLUSION The three methods used (chemical, mechanical DQG FRPELQHG ZHUH HTXDOO\ HIIHFWLYH ZLWK UHVSHFW WR WKH DELOLW\ WR UHPRYH ELR¿OP DQG ZHUH VXSHULRU WR WKH HVWDEOLVKHG FRQWURO PHWKRG EUXVKLQJ ZLWK ZDWHU FRQWULEXWLQJ WR WKH PDLQWHQDQFH RI RUDO KHDOWK FDUH RI WKH FRPSOHWH GHQWXUH ZHDUHUV
Australian Endodontic Journal, 2012
The effectiveness of sonic activation, laser activation and syringe irrigation of 4% sodium hypochlorite in removing an Enterococcus faecalis biofilm was compared. Biofilms were grown in extracted human single rooted teeth using a flow cell apparatus. After 4 weeks' growth, teeth were subjected to each treatment using 4% sodium hypochlorite and radicular dentinal surfaces of the root canals were analysed by scanning electron microscopy. Results showed that sonic activation and syringe irrigation with sodium hypochlorite showed reduced numbers of bacterial cells on the radicular dentine but were not effective in eliminating E. faecalis in the dentinal tubules. Laser activation of sodium hypochlorite resulted in clean dentine walls and undetectable levels of bacteria within dentinal tubules. Qualitatively, sonic or laser activation of 4% NaOCl resulted in greater bacterial reduction compared with syringe irrigation, with laser activation producing the greatest overall reduction. Aust Endod J 2012 bs_bs_banner 1
F1000Research
Background: Sodium hypochlorite (NaOCl) is the most commonly used irrigant in endodontics. The purpose of this study was to evaluate the effect of NaOCl solution (2.5%) and gel (3%) with/without passive ultrasonic irrigation (PUI) on Enterococcus faecalis, Escherichia coli, and their endotoxins, lipopolysaccharide (LPS) and lipoteichoic acid (LTA). Methods: 40 human lower premolars were contaminated with E. coli (ATCC 25922) for 28 days and E. faecalis (ATCC 29212) for 21 days. Specimens were randomly divided into four groups: (1) 2.5% NaOCl irrigating the canals without PUI activation; (2) 2.5% NaOCl with PUI; (3) 3% NaOCl gel irrigating the canals without PUI; and (4) 3% NaOCl gel with PUI. 40 mL of irrigant was used for each group. PUI activation was carried out using E1-Irrisonic stainless-steel tip at 10% frequency. After treatment, all specimens were filled with 3mL of 17% ethylenediaminetetraacetic acid (EDTA) for 3min and then washed with nonpyrogenic saline solution. Three ...
Bioengineering
Purpose: This study aimed to compare the antibacterial effectiveness of passive ultrasonic irrigation (PUI), Er,Cr:YSGG laser (WTL), and photon-induced photoacoustic streaming (PIPS) using an Er:YAG laser against Enterococcus faecalis biofilms in the apical third of root canals. Methods: Root canals of 70 single-rooted human teeth were instrumented and infected with E. faecalis for 3 weeks to form biofilms. The samples were randomly divided into five groups as follows: (i) PUI + 3% NaOCl (n = 16); (ii) Er,Cr:YSGG laser (n = 16); (iii) PIPS + 3% NaOCl (n = 16); (iv) positive control group (n = 10); and (v) negative control group (n = 10). The bacterial content in the root canal was sampled using (a) the paper-point sampling method before (S1) and after (S2) treatment and (b) pulverising the apical 5 mm of the root. The number of bacteria recovered from each group was counted as colony-forming units (CFUs). The amount of reduction between the groups was compared with the Kruskal–Walli...
Photodiagnosis and photodynamic therapy, 2015
The aim of this study was to evaluate the effect of photodynamic therapy (PDT) on Enterococcus faecalis biofilms in artificially infected root canals using modified photosensitizers and passive ultrasonic activation. Two hundred and seventy extracted human teeth with one root canal were instrumented utilizing ProTaper files, autoclaved, infected with E. faecalis T9 for 72hours and divided into different groups: irrigation with 3% sodium hypochlorite (NaOCl), 20% ethylenediaminetetraacetic acid (EDTA), or 20% citric acid, PDT without irrigation, PDT accompanied by irrigation with NaOCl, EDTA, or citric acid, PDT using an EDTA-based photosensitizer or a citric-acid-based photosensitizer and PDT with ultrasonic activation of the photosensitizer. A 15mg/ml toluidine blue served as the photosensitizer, activated by a 100 mW LED light source. Sterile paper points were used for sampling the root canals and dentin chips were collected to assess the remaining contamination after treatment. S...