An In Vitro Anticancer, Antioxidant, and Phytochemical Study on Water Extract of Kalanchoe daigremontiana Raym.-Hamet and H. Perrier (original) (raw)
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Phenolic acid content, antioxidant and cytotoxic activities of four Kalanchoë species
Saudi Journal of Biological Sciences, 2016
Phenolic acid composition, antioxidant, and cytotoxic activities in leaves of four Kalanchoe (Crassulaceae) species were evaluated. Determination of phenolic acid contents were conducted by an optimized LC-ESI-MS/MS method. The results show that Kalanchoe daigremontiana Raym.-Hamet & H. Perrier (using ASE extraction) and Kalanchoe pinnata (Lam.) Pers. contain the highest amounts of phenolic acids, while Kalanchoe nyikae Engl. the lowest ones. Among phenolic acids ferulic, caffeic and protocatechuic acids were occurring in the highest quantities in the analysed species. The greatest amounts of ferulic and protocatechuic acids were found in K. daigremontiana and K. pinnata. Moreover, the antiradical and cytotoxic activities of Kalanchoe extracts were investigated. All tested extracts possessed antioxidant activity. The obtained IC 50 values (lg/mL) ranged from 49.9 lg/mL to 1410 lg/mL, indicating a large variation of the activity of the analysed extracts. Cytotoxicity assays revealed dose-dependent effects in the cells lines tested. Only K. pinnata extract showed a high cytotoxicity against the H-9 human T cell line. Other extracts (K. daigremontiana, Kalanchoe milloti, K. nyikae) showed more pronounced cytotoxicity towards J45.01 cells (human acute lymphoblastic leukaemia T cells). The present study demonstrated that Kalanchoe extracts have significant antioxidant and cytotoxic effects. This suggests that these species can be used as new sources of natural antioxidants and potential anticancer compounds.
Akta Kimia Indonesia
Kalanchoe plant, known as "sosor bebek" in Indonesia is a perennial herb and has succulent leaves. The plant is known in folklore and traditional medicine in Indonesia for the treatment of fever, abscesses, bruises, contused wounds, coughs and skin diseases. During the course of our continuing search for novel cytotoxic compounds, the methanolic extract of Kalanchoe tomentosa plants showed cytotoxic activity against P-388 murine leukemia cells. The methanolic extract of the fresh leaves of K. tomentosa was concentrated and extracted successively with n-hexane, ethyl acetate and n-butanol. The ethyl acetate extract exhibited strongest cytotoxic activity againts P-388 murine leukemia cells. By using the cytotoxic activity to follow the separation, the ethyl acetate fraction was separated by combination of column chromatography on silica gel and preparative TLC on silica gel GF 254 to afford a kaempferol-3-O-glycosides (1) and kaempferol-3-O-rhamnoside (2). Compound 1 and 2 showed cytotoxic activity against P-388 murine leukemia cells with IC 50 > 100μg/mL and IC 50 3.32 μg / mL.
Pharmaceutical Biology
Context: Kalanchoe species (Crassulaceae) are widely used in traditional medicine as remedies in infectious diseases and cancer treatment. Objective: Cytotoxic and antimicrobial activities of Kalanchoe daigremontiana Raym.-Hamet & H. Perrier, K. pinnata (Lam.) Pers., and K. blossfeldiana Poelln. extracts were determined. The relationship between biological activities and the extracts bufadienolides content was also investigated. Materials and methods: Fresh leaves of Kalanchoe species were macerated with 95% ethanol or water. The quantitative analysis of bufadienolides in the extracts was carried out with mass spectrometry. Cytotoxicity tests were performed on human cancer cell lines-HeLa, SKOV-3, MCF-7, and A375 by MTT (3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide) assay and Real-Time Cell Analysis system. The microbiological study was done using a few bacteria strains (b-hemolytic Streptococcus, Corynebacterium diphtheriae, Staphylococcus aureus, Staphylococcus epidermidis, Enterococcus hirae, Escherichia coli) and Candida albicans. Results: The K. blossfeldiana ethanol extract and K. daigremontiana water extract exhibited the most potent cytotoxic activity (IC 50 < 19 mg/mL for HeLa and SKOV-3 cells). The strongest antibacterial effects showed ethanol extract of K. blossfeldiana and K. pinnata (MIC values were 8.45, 8.45, 0.25 and <33.75 mg/mL for S. aureus, S. epidermidis, and E. hirae, respectively). The highest total amount of bufadienolides was in K. daigremontiana ethanol extract. In contrast, K. blossfeldiana ethanol extract did not show the presence of these compounds. Conclusions: Kalanchoe blossfeldiana ethanol extract is a potential candidate for cancer and bacterial infection treatment. Additionally, the biological effects of Kalanchoe extracts are not dependent on the presence and amount of bufadienolides in the plant extracts.
DNA profiling of two closely related ornamental plants belonging to family crassulaceae viz. Kalanchoe thrysiflora Harv. and Kalanchoe marmorata Baker were performed to establish genetic polymorphism. Biological guided fractionation of the two plant extracts to assess their cytotoxicity, had led to the isolation of one steroidal and one triterpenoidal compound from the most active dichloromethane fraction of Kalanchoe thrysiflora. The cytotoxicity of the isolated compounds were evaluated against normal (HFB4) and cancer (MCF7) cells. Compound 1 (3-oxo-olean-12-ene) and compound 2 (β-sitosterol) showed similar cytotoxic activity on MCF7 at IC 50 17.4 and 17.6 µg/ml respectively while on HFB4, the compounds revealed cytotoxic activity at IC 50 21.9 and 21.6 respectively.
Journal of Toxicology
The species Kalanchoe brasiliensis, known as “Saião,” has anti-inflammatory, antimicrobial, and antihistamine activities. It also has the quercetin and kaempferol flavonoids, which exert their therapeutic activities. With extensive popular use besides the defined therapeutical properties, the study of possible side effects is indispensable. The objective of this study is to evaluate the toxicity in vitro and in vivo from the hydroethanolic extract of the leaves of K. brasiliensis. The action of the extract (concentrations from 0.1 to 1000 uL/100 uL) in normal and tumor cells was evaluated using the MTT method. Acute toxicity and subchronic toxicity were evaluated in mice with doses of 250 to 1000 mg/kg orally, following recognized protocols. The in vitro results indicated cytotoxic activity for 3T3 cell line (normal) and 786-0 (kidney carcinoma), showing the activity to be concentration-dependent, reaching 92.23% cell inhibition. In vivo, the extract showed no significant toxicity; ...
2021
The aim of this study was to analyze the chemical components and evaluate the biological activity of the extracts from the leaves Kalanchoe pinnata and Kalanchoe daigremontiana, which are cultivated in the province of Chiriqui, Republic of Panama. Phytochemicals components, antioxidant and anti-inflammatory activities were studied. The composition of the obtained petroleum ether, ethanol and aqueous extracts was analyzed by phytochemical screening. The antioxidant activity of the extracts was studied using three in vitro model systems (DPPH radical scavenging assay, nitric oxide radical scavenging assay, and superoxide radical scavenging activity). The anti-inflammatory activity of these species was studied using an in vivo model (ʎ-carrageenan-induced paw edema in rats). Phytochemical analysis of the extracts showed the presence of alkaloids, steroids, triterpenes, flavonoids, phenolic compounds, saponins and glycosides. The greatest radical inhibitory effect was observed in the DP...
Natural Product Sciences
Seven flavonoid compounds, kaempferol (1), quercetin (2), quercetin-3-O-β-D-glucopyranoside (3), kaempferol-3-O-β-D-glucopyranoside (4), kaempferol-3-O-α-L-rhamnoside (5), quercetin-3-O-sophoroside (6) and quercetin-3-O-rutinoside (7), were isolated from the methanolic extract of leaves of Kalanchoe prolifera. Compounds 1-7 were isolated for first time from this plant. These compounds were evaluated their cytotoxic activity against P-388 murine leukimia cells in vitro. Among those compounds kaempferol (1) and quercetin (2) showed strongest cytotoxic activity with IC 50 values of 4.45 ± 0.05 and 6.28 ± 0.02 μg/mL, respectively.
Phenolics from Kalanchoe marmorata Baker, Family Crassulaceae
Bulletin of Faculty of Pharmacy, Cairo University, 2011
DNA profiling of two closely related ornamental plants belonging to family crassulaceae viz. Kalanchoe thrysiflora Harv. and Kalanchoe marmorata Baker were performed to establish genetic polymorphism. Biological guided fractionation of the two plant extracts to assess their cytotoxicity, had led to the isolation of one steroidal and one triterpenoidal compound from the most active dichloromethane fraction of Kalanchoe thrysiflora. The cytotoxicity of the isolated compounds were evaluated against normal (HFB4) and cancer (MCF7) cells. Compound 1 (3-oxo-olean-12-ene) and compound 2 (β-sitosterol) showed similar cytotoxic activity on MCF7 at IC 50 17.4 and 17.6 µg/ml respectively while on HFB4, the compounds revealed cytotoxic activity at IC 50 21.9 and 21.6 respectively.
African Journal of Pure and Applied Chemistry, 2012
The research was conducted to determine the antioxidant activity of two Kalanchoe species in Ghana which are used widely for the treatment of various ailments including stroke and ulcers. The leaves were subjected to methanol and aqueous extractions for each sample. Thin layer chromatography (TLC) was performed to determine the various components in the leaves extract. The concentration of phenol and flavonoid was determined using folin-ciocalteu Zishen et al. (2003) and Lee et al. (2005) method. The extract was also screened to determine the total flavonoid and phenolic content of the methanol and aqueous extracts of the leaves of Kalanchoe pinnata and Kalanchoe integra and also to assess the antioxidant activity. 1,1-Diphenyl-2-picrylhydryzyl [DPPH] radical scavenging activity of both aqueous and methanol extracts of K. integra and K. pinnata were determined by the Brand-William method (2003)
.PRELIMINARY PHYTOCHEMICAL STUDIES OF KALANCHOE
Kalanchoe gastonis-bonnieri is a succulent plant native of Madagascar. The leaves have been used in traditional system of medicine for the treatment of ovarian cysts, urinary tract infections, vaginal infections, uterine fibroids. Unfortunately very few works related to the phytochemistry of this species is reported in literatures. The present investigation was carried out to reveal the important phytochemicals present in the leaf extract. The preliminary screening showed the presence of many phytochemicals such as alkaloids, flavonoids, terpenoids, saponoin, fixed oils and fats. High Performance Thin Layer Chromatography analysis was carried out with optimized solvent system consisting of ethyl acetate, formic acid, glacial acetic acid and water in the ratio of 8:1:1:2. The densitometric scanning of the chormatograms of hydroaclcoholic extract showed 7peaks at 254 nm 8 peaks at 366 nm. The phytochemicals detected in the present study justifies the therapeutic uses of the leaves in the traditional medicines.