Phenolic acid content, antioxidant and cytotoxic activities of four Kalanchoë species (original) (raw)
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African Journal of Pure and Applied Chemistry, 2012
The research was conducted to determine the antioxidant activity of two Kalanchoe species in Ghana which are used widely for the treatment of various ailments including stroke and ulcers. The leaves were subjected to methanol and aqueous extractions for each sample. Thin layer chromatography (TLC) was performed to determine the various components in the leaves extract. The concentration of phenol and flavonoid was determined using folin-ciocalteu Zishen et al. (2003) and Lee et al. (2005) method. The extract was also screened to determine the total flavonoid and phenolic content of the methanol and aqueous extracts of the leaves of Kalanchoe pinnata and Kalanchoe integra and also to assess the antioxidant activity. 1,1-Diphenyl-2-picrylhydryzyl [DPPH] radical scavenging activity of both aqueous and methanol extracts of K. integra and K. pinnata were determined by the Brand-William method (2003)
2021
The aim of this study was to analyze the chemical components and evaluate the biological activity of the extracts from the leaves Kalanchoe pinnata and Kalanchoe daigremontiana, which are cultivated in the province of Chiriqui, Republic of Panama. Phytochemicals components, antioxidant and anti-inflammatory activities were studied. The composition of the obtained petroleum ether, ethanol and aqueous extracts was analyzed by phytochemical screening. The antioxidant activity of the extracts was studied using three in vitro model systems (DPPH radical scavenging assay, nitric oxide radical scavenging assay, and superoxide radical scavenging activity). The anti-inflammatory activity of these species was studied using an in vivo model (ʎ-carrageenan-induced paw edema in rats). Phytochemical analysis of the extracts showed the presence of alkaloids, steroids, triterpenes, flavonoids, phenolic compounds, saponins and glycosides. The greatest radical inhibitory effect was observed in the DP...
Journal of Pharmacy and Nutrition Sciences, 2012
Many diseases are associated with oxidative stress caused by free radicals. Current research is directed towards finding naturally occurring antioxidants of plant origin. Kalanchoe crenata Andr. (Crassulaceae), commonly known as "never die" or "Dog's liver," has been traditionally used for the treatment of ailments, such as, earache, smallpox, headache, inflammation, pain, asthma, palpitations, convulsion, and general debility. The aim of present research deals with phytochemical screening and in-vitro evaluation of antioxidant activities of the leaves & stems of K.crenata. Method: Successive extracts of leaves & stems was subjected for phytochemical screening. The preliminary screening reports the presence of saponins, phytosterols, flavanoids, phenols and alkaloids in the extracts. Various extracts of K.crenata leaves & stems was studied in-vitro for total antioxidant activity, for scavenging of nitric oxide, hydrogen peroxide, the antioxidant capacity by p...
Der Pharmacia Lettre, 2016
Kalanchoe crenata (Adrews) Haw. is an ornamental plant belonging to the family Crassulaceae is probably native to Africa and drier parts of southeast Asia. Commonly known as "never die" or "Dog's liver", this plant is widely used in traditional medicine in the treatment of inflammation, earache, headache, asthma, palpitation, abdominal pain, convulsion and general debility [1]. The leaves of K.crenata possess analgesic, anticonvulsant & spasmolytic properties [2, 3, 4] .The present research includes isolation and characterization of chemical constituent and in vitro evaluation of antioxidant potentials by assay method of isolated constituent from leaves of Kalanchoe crenata. Chemical constituent (hentriacontan-1-ol) was isolated and the structures of this compound have been established by spectroscopic methods (UV, 1 H-NMR, IR, MS). This constituent was isolated from this plant for the first time. This chemical constituent showed significant antioxidant activity in dose dependent manner assay method. The result obtained in the present study indicates that leaves of Kalanchoe crenata could be a potential source of natural antioxidant. This also justified the traditional use of herb in preventing disease induced by oxidative stress.
Journal of Toxicology
The species Kalanchoe brasiliensis, known as “Saião,” has anti-inflammatory, antimicrobial, and antihistamine activities. It also has the quercetin and kaempferol flavonoids, which exert their therapeutic activities. With extensive popular use besides the defined therapeutical properties, the study of possible side effects is indispensable. The objective of this study is to evaluate the toxicity in vitro and in vivo from the hydroethanolic extract of the leaves of K. brasiliensis. The action of the extract (concentrations from 0.1 to 1000 uL/100 uL) in normal and tumor cells was evaluated using the MTT method. Acute toxicity and subchronic toxicity were evaluated in mice with doses of 250 to 1000 mg/kg orally, following recognized protocols. The in vitro results indicated cytotoxic activity for 3T3 cell line (normal) and 786-0 (kidney carcinoma), showing the activity to be concentration-dependent, reaching 92.23% cell inhibition. In vivo, the extract showed no significant toxicity; ...
.PRELIMINARY PHYTOCHEMICAL STUDIES OF KALANCHOE
Kalanchoe gastonis-bonnieri is a succulent plant native of Madagascar. The leaves have been used in traditional system of medicine for the treatment of ovarian cysts, urinary tract infections, vaginal infections, uterine fibroids. Unfortunately very few works related to the phytochemistry of this species is reported in literatures. The present investigation was carried out to reveal the important phytochemicals present in the leaf extract. The preliminary screening showed the presence of many phytochemicals such as alkaloids, flavonoids, terpenoids, saponoin, fixed oils and fats. High Performance Thin Layer Chromatography analysis was carried out with optimized solvent system consisting of ethyl acetate, formic acid, glacial acetic acid and water in the ratio of 8:1:1:2. The densitometric scanning of the chormatograms of hydroaclcoholic extract showed 7peaks at 254 nm 8 peaks at 366 nm. The phytochemicals detected in the present study justifies the therapeutic uses of the leaves in the traditional medicines.
International Journal of Green Pharmacy, 2012
Background: Hopea ponga has been categorised as an endangered tree species under International Union for Conservation of Nature red list of threatened species. Objective: To study the antibacterial, antioxidant activities, total phenolic and flavonoid contents of Hopea ponga leaf, extracted from different solvents viz., petroleum ether, chloroform and methanol. Materials and Methods: Antibacterial activity of leaf extract of Hopea ponga was determined by disc diffusion method against Escherichia coli, Proteus vulgaris, Bacillus cereus, Streptococcus faecalis and Pseudomonas aeruginosa. The antioxidant activities of the extracts were assayed through in-vitro models such as 2,2-diphenyl-1-picrylhydrazyl, 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid), ferric reducing antioxidant power, hydrogen peroxide and hydroxyl scavenging activities. Total flavonoid content was determined using aluminium chloride colorimetric method and total phenolic content determined using Folin ciocaulteu reagent method. Results: Methanol extract showed higher scavenging activity against 2,2-diphenyl-1-picrylhydrazyl and reducing power activity against ferric ion (97.24 ± 0.11 and 88.01 ± 0.03) compared to other extracts. Methanol extract showed significant antibacterial activity against P. vulgaris and B. cereus. Among the tested extracts, methanol extract exhibited higher content of phenols (1318.749 mg of ferulic acid equivalent/100 gram dry weight of extract) and flavonoids (457.832 mg of quercetin equivalent/100 gram dry weight of extract). Conclusions: The present study suggests that the methanol extract may contain active compounds, which have effective antioxidant and antimicrobial activity. Hence, this endangered species have to be explored and conserved to serve the mankind.
Akta Kimia Indonesia
Kalanchoe plant, known as "sosor bebek" in Indonesia is a perennial herb and has succulent leaves. The plant is known in folklore and traditional medicine in Indonesia for the treatment of fever, abscesses, bruises, contused wounds, coughs and skin diseases. During the course of our continuing search for novel cytotoxic compounds, the methanolic extract of Kalanchoe tomentosa plants showed cytotoxic activity against P-388 murine leukemia cells. The methanolic extract of the fresh leaves of K. tomentosa was concentrated and extracted successively with n-hexane, ethyl acetate and n-butanol. The ethyl acetate extract exhibited strongest cytotoxic activity againts P-388 murine leukemia cells. By using the cytotoxic activity to follow the separation, the ethyl acetate fraction was separated by combination of column chromatography on silica gel and preparative TLC on silica gel GF 254 to afford a kaempferol-3-O-glycosides (1) and kaempferol-3-O-rhamnoside (2). Compound 1 and 2 showed cytotoxic activity against P-388 murine leukemia cells with IC 50 > 100μg/mL and IC 50 3.32 μg / mL.
Phenolics from Kalanchoe marmorata Baker, Family Crassulaceae
Bulletin of Faculty of Pharmacy, Cairo University, 2011
DNA profiling of two closely related ornamental plants belonging to family crassulaceae viz. Kalanchoe thrysiflora Harv. and Kalanchoe marmorata Baker were performed to establish genetic polymorphism. Biological guided fractionation of the two plant extracts to assess their cytotoxicity, had led to the isolation of one steroidal and one triterpenoidal compound from the most active dichloromethane fraction of Kalanchoe thrysiflora. The cytotoxicity of the isolated compounds were evaluated against normal (HFB4) and cancer (MCF7) cells. Compound 1 (3-oxo-olean-12-ene) and compound 2 (β-sitosterol) showed similar cytotoxic activity on MCF7 at IC 50 17.4 and 17.6 µg/ml respectively while on HFB4, the compounds revealed cytotoxic activity at IC 50 21.9 and 21.6 respectively.