Issn 2347-3614 Hepatoprotective & Antioxidant Effect of Whole Plant Extract of Polygonumglabrum Willd . On CCL 4 Induced Hepatic Damage in Rats (original) (raw)
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Spatula DD - Peer Reviewed Journal on Complementary Medicine and Drug Discovery, 2012
The aim of this study was to investigate the antioxidant and hepatoprotective activity of ethanolic extract of the leaves of Polygonum glabrum in carbon tetrachloride (CCl4) induced hepatotoxicity in rats. METHODS: Rats were pretreated orally for 8 days with the ethanolic leaf extract of P. glabrum (200 and 400 mg/kg) and curcumin (100 mg/kg) as the standard prior to CCl4 (1ml/kg body weight, intraperitonial) intoxication. Hepatoprotective action was assessed based on the decrease in the levels of hepatic serum markers such as serum glutamate pyruvate transaminase (SGPT), serum glutamate oxaloacetate transaminase (SGOT), alkaline phosphatase (ALP), total bilirubin (TB), direct bilirubin (DB) and increased total protein levels. Further, liver tissue was subjected to histopathological studies and also assessed for oxidative parameters such as malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT) and glutathione (GSH) levels. RESULTS: Pretreatment with curcumin and ethanolic leaf extract of P. glabrum significantly inhibited CCl4 induced increase of SGPT, SGOT, ALP, TB and DB levels in serum and decrease in total protein levels. Significant and dose dependent increase in the hepatic SOD, CAT, GSH activities along with decrease in MDA levels were observed in the curcumin and P. glabrum extract pretreated animals. The liver histological examination also showed lowered damage in the extract pretreated animals. CONCLUSION: The ethanolic leaf extract of P. glabrum exhibited significant hepatoprotective effect which could be attributed to its antioxidant activity.
Carbon tetrachloride (CCl 4 ) intoxicated rats showed significant elevation in serum enzymes, bilirubin and lipid peroxidation of the liver tissues and reduction in serum total protein, superoxide dismutase, catalase, reduced glutathione peroxide activity. Treatment with ethanol extract of Polygala rosmarinifolia whole plant altered the above parameters to the levels of near normal. All the above results were comparable with the standard drug silymarin (100mg/kg) treated group. Thus the present study ascertains that the ethanol extract of Polygala rosmarinifolia whole plant possesses significant hepatoprotective activity.
2013
The leaves of Artemisia princeps Pamp have been used for tea and food in Japan. The polyphenols of the leaves have inhibitory effects against lipid peroxidation and protein fragmentation by free radicals in vitro and an inhibitory effect on galactosaminelipopolysaccharide induced hepatotoxicity in vivo. The levels of serum glutamic oxaloacetic transaminase and glutamic pyruvic transaminase, lipid peroxidation in serum and liver by hepatotoxity were depressed by polyphenols in A. princeps Pamp. The depression of gluthathione and superoxide dismutase in plasma and liver by hepatotoxity were elevated by polyphenols in A. princeps Pamp. These results demonstrated that polyphenols in A. princeps Pamp have antioxidant and hepatoprotective effects.
journal of applied pharmaceutical science, 2013
Article history: Received on: 12/12/2012 Revised on: 19/01/2013 Accepted on: 05/02/2013 Available online: 27/02/2013 The aim of the study was to investigate the hepatoprotective and antioxidant potential of methanolic extract of Polyalthiya longifolia fruits (MEPL) by in-vitro and in-vivo methods.In the invitro study, Freshly isolated rat primary hepatocytes and HepG2 cells were exposed with CCl4 along with/without various concentrations of MEPL(125,250,500 μg/kg) and the effects were studied.In the in-vivo studies, CCl4 intoxication method was used and aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), total bilirubin and total proteins were estimated and supported with histopathological studies.MEPL (125,250,500 μg/kg) treated animals increased the percentage of viability in both primary hepatocytes (p<0.001) and in HepG2 cells (p<0.01) where as in the invivo studies,MEPL produced significant hepatoprotective effect by decreasing th...
2011
The present study was carried out to observe the hepatoprotective effect and antioxidant activity of the aqueous extract of the roots of Polygonum bistorta Linn. (100 mg/kg) and tannic acid (25 mg/kg) in rats treated with carbon tetrachloride (0.15 ml/kg, i.p.). Plant extract and TA at the tested doses restored the levels of all serum (aspartate aminotransferase, alanine aminotransferase, alkaline phosphatase, albumin, total bilirubin and total protein) and liver homogenate enzymes superoxide dismutase, glutathione reductase, glutathione peroxidase, glucose-6-phosphatase dehydrogenase and drug-metabolizing enzymes (aniline hydroxylase and amidopyrine-N-demethylase) significantly. Histology demonstrated profound steatosis degeneration and nodule formation were observed in the hepatic architecture of carbon tetrachloride treated rats which were found to acquire near to normal in extract and TA with carbon tetrachloride administrated rats, and supported the biochemical observations. This study suggests that tannic acid has a more liver protective effect in comparison to extract of P. bistorta against carbon tetrachloride-induced hepatotoxicity and possess antioxidant activities.
Hepatoprotective effects of methanol extract of Carissa opaca leaves on CCl4-induced damage in rat
BMC Complementary and Alternative Medicine, 2011
Background: Carissa opaca (Apocynaceae) leaves possess antioxidant activity and hepatoprotective effects, and so may provide a possible therapeutic alternative in hepatic disorders. The effect produced by methanolic extract of Carissa opaca leaves (MCL) was investigated on CCl 4-induced liver damages in rat. Methods: 30 rats were divided into five groups of six animals of each, having free access to food and water ad libitum. Group I (control) was given olive oil and DMSO, while group II, III and IV were injected intraperitoneally with CCl 4 (0.5 ml/kg) as a 20% (v/v) solution in olive oil twice a week for 8 weeks. Animals of group II received only CCl 4. Rats of group III were given MCL intragastrically at a dose of 200 mg/kg bw while that of group IV received silymarin at a dose of 50 mg/kg bw twice a week for 8 weeks. However, animals of group V received MCL only at a dose of 200 mg/kg bw twice a week for 8 weeks. The activities of aspartate transaminase (AST), alanine transaminase (ALT), alkaline phosphatase (ALP), lactate dehydrogenase (LDH) and γ-glutamyltransferase (γ-GT) were determined in serum. Catalase (CAT), peroxidase (POD), superoxide dismutase (SOD), glutathione-S-transferase (GST), glutathione peroxidase (GSH-Px), glutathione reductase (GSR) and quinone reductase (QR) activity was measured in liver homogenates. Lipid peroxidation (thiobarbituric acid reactive substances; TBARS), glutathione (GSH) and hydrogen peroxide (H 2 O 2) concentration was also assessed in liver homogenates. Phytochemicals in MCL were determined through qualitative and high performance liquid chromatography (HPLC) analysis. Results: Hepatotoxicity induced with CCl 4 was evidenced by significant increase in lipid peroxidation (TBARS) and H 2 O 2 level, serum activities of AST, ALT, ALP, LDH and γ-GT. Level of GSH determined in liver was significantly reduced, as were the activities of antioxidant enzymes; CAT, POD, SOD, GSH-Px, GSR, GST and QR. On cirrhotic animals treated with CCl 4 , histological studies showed centrilobular necrosis and infiltration of lymphocytes. MCL (200 mg/kg bw) and silymarin (50 mg/kg bw) co-treatment prevented all the changes observed with CCl 4-treated rats. The phytochemical analysis of MCL indicated the presence of flavonoids, tannins, alkaloids, phlobatannins, terpenoids, coumarins, anthraquinones, and cardiac glycosides. Isoquercetin, hyperoside, vitexin, myricetin and kaempherol was determined in MCL. Conclusion: These results indicate that MCL has a significant protective effect against CCl 4 induced hepatotoxicity in rat, which may be due to its antioxidant and membrane stabilizing properties.
International Journal of Pharmacy and Pharmaceutical Sciences, 2016
Objective: The present investigations were undertaken to evaluate the hepatoprotective and antioxidant activity of the ethanolic extract of the whole plant of Combretum albidum G Don against CCl4-induced hepatotoxicity in rats. Methods: Hepatoprotective effect of ethanolic extract of Combretum albidum (EECA) was determined by using carbon tetrachloride (CCl4) intoxication of rats as experimental models. The extent of liver damage and effect of the plant extract was assessed by various biochemical parameters like alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), total billrubin (TB) and total protein (TP) in blood serum and concentration of thiobarbituric acid reactive substances (TBARS), reduced glutathione (GSH), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), glutathione-s-transferase (GST) in liver were determined. Histopathological changes in the liver of different groups were also studied. Results: The administration of EECA at dose levels of 250 and 500 mg/kg/b.w., orally had decreased the rise of ALT, AST, ALP, TB and TBRAS levels and the effects were comparable to standard drug (Silymarin 25 mg/kg/b. w,) the GSH, SOD, CAT, GPx, GST and TP levels were significantly increased in the animals received EECA. The histopathological studies show decreased necrosis and hepatocellular degeneration when compared to the CCl4 intoxicated liver. Conclusion: This study demonstrates that the hepatoprotective and the antioxidant activity of the whole plant of Combretum albidum therefore scientifically supports the use of this plant in traditional medicine for treatment of liver disorders.
ijpbs.net
The Poly herbal ethanolic extract (PHEE) was evaluated for its anti hepatotoxic potential against carbon tetrachloride (CCL 4 ) induced hepatic damage in male albino rats. Ethanolic extract from the leaves of Melia azadirachta, seeds of Piper longum and whole plant of Eclipta alba at a dose level of 50 mg/kg body weight was administered orally daily once for 14 days. The substantially reduced levels of enzymatic anti-oxidants such as superoxide dismutase(SOD), catalase(CAT), glutathione peroxidase (GPx), glutathione-stransferase(GST) and glutathione reductase (GR), due to carbon tetra chloride effect were restored to normal. Histopathological examination of liver sections of PHEE treated rats shows the restoration of normal tissues. Vitamin-E at a dose of 100 mg/kg taken as a standard reference also exhibited significant antioxidant activity against CCl 4 induced Hepatotoxicity. The results of this study strongly justified that PHEE has a potent hepatoprotective activity against carbon tetrachloride
Lekovite sirovine, 2019
The influence of Satureja montana extracts on several liver biochemical parameters in mice administered with carbon tetrachloride was estimated. In vivo investigation of antioxidant properties of S. montana extracts encompassed monitoring of biochemical parameters (derived from liver homogenate and blood hydrolyzate) and examination of potential hepatoprotective effect after intoxication with carbon tetrachloride. Biochemical tests included determination of activity of several antioxidant enzymes: xanthine oxidase, catalase, peroxidase, glutathione peroxidase, amount of reduced glutathione and intensity of lipid peroxidation. In order to obtain data about the potent hepatoprotective effect of examined extracts, the following parameters were determined: aspartate aminotransferase, alanine aminotransferase, bilirubin, hepatic DNA and hydroxyprolin. Results of this study demonstrate that S. montana extracts inhibited the hepatotoxicity produced by carbon tetrachloride administration most probably through activation of physiological common defense mechanisms.