Comparative Study of Essential Oil Yields of Seven Accessions of Artemisia Herba-Alba Asso, Domesticated in Errachidia (Southeast of Morocco) (original) (raw)
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Natural Products: An Indian Journal, 2012
Artemisia mesatlantica is an aromatic plant, spontaneous and widespread in Morocco. It is used by the local populations for its medicinal virtues. The purpose of this present work is the comparative study of the yield according to the date of harvest. Additionally, we sought to determine the chemical composition of the essential oil stemming from this species, as well as the determination of its antimicrobial activities towards eleven pathogens. Our samples result from a rural area in eastern Morocco, namely a region between Ifrane and Boulmane. Seventy eight constituents were identified by chromatographic analysis (GC and GC/SM) in essential oil of this plant among which four are preponderant: âÂÂthujone, 1,8 cineole, camphene and camphor. The best yield in essential oil of the Artemisia mesatlantica is in June and it is in the order of 1 % (mg/100g). The essential oil of Artemisia mesatlantica showed a significant antimicrobial activity against four species of bacteria, three spe...
Journal of Applied Pharmaceutical Science, 2016
This study was conceived to investigate the composition of four essential oils (EOs) extracted by hydrodistillation from four parts (leaves, stems, leaves/stems, roots) of Artemisia herba-alba growing wild in the Center of Tunisia. For this, Artemisia herba-alba aerial and roots parts were shade dried with ventilation at room temperature. Then, plant different parts were cut into small pieces and subjected to hydrodistillation using a Clevenger-type apparatus. The gas chromatography (GC) analyses were accomplished with a HP-5890 Series II instrument. The main results showed a total of 152 compounds detected and identified by GC and GC-MS and accounting for 91.3-99.7% of the whole oil. The four oils were characterized by the predominance of monoterpene derivatives (68.2-99.5%) and the major volatile constituent was α-thujone (18.2-45.5%). Qualitative and quantitative differences between the four essential oils have been noted for some compounds. The main compounds of leaves essential oil were α-Thujone (45.5%), β-Thujone (11.4%), trans-sabinyl acetate (10.1%), 1,8-Cineole (7.4%) and camphor (6.8%). α-Thujone (27.5%) was also the main compound in the essential oil of leaves/stems, followed by camphor (22.9%), 1,8-cineole (8.3%), β-thujone (8.2%) and camphene (5.6%). The essential oil of stems was dominated by α-Thujone (28%) followed by β-Thujone (11.4%) and chrysantenone (11%). In the essential oil of roots, α-thujone was less represented (18.2%), followed by camphor (14.6%) and curcumen-15-al (14.3%). It is important to mention that curcumen-15-al has been reported for the first time in Artemisia herba-alba oil Our results revealed avariability in the chemical composition and the yield of the EOs from Artemisia herba-alba. Moreover, curcumen-15-al is a new chemotype first found in Artemisia herba-alba from Tunisia.
Chemical variability of Artemisia herba-alba Asso essential oils from East Morocco
Chemical Papers, 2010
Chemical compositions of 16 Artemisia herba-alba oil samples harvested in eight East Moroccan locations were investigated by GC and GC/MS. Chemical variability of the A. herba-alba oils is also discussed using statistical analysis. Detailed analysis of the essential oils led to the identification of 52 components amounting to 80.5–98.6 % of the total oil. The investigated chemical compositions showed significant qualitative and quantitative differences. According to their major components (camphor, chrysanthenone, and α- and β-thujone), three main groups of essential oils were found. This study also found regional specificity of the major components.
Assessment of essential oil yield of Artemisia herba-alba cultivated in Tunisian arid zone
The main objective of this study is to estimate the essential oil (EO) yield of Artemisia herba-alba through biomass prediction. EO yield dependent in both the biomass production and its EO content, was determined from the recovered biomass after harvest of the upper half of plant tuft. The bio-volume parameter of twenty cultivated individuals of A. herba-alba were estimated four times during four years from height and diameters measurements. In the first harvest, after one year of establishment, several individuals appeared more productive than others. Thus, the canopy diameters of the plant tuft and the bio-volume recorded little significant relationship with the EO yield (R 2 = 0.47 and 0.57, respectively). Harvesting the upper half of plant tuft, the spherical volume model developed by the regenerated material of the same individuals resulted in a high correlation between the EO yield and both the mean diameter (R 2 = 0.61 to 0.62) and the bio-volume (R 2 = 0.66 to 0.83).
As part of the valorization of medicinal and aromatic plants in Morocco, we studied the effect of the provenance on the yield, chemical composition and antimicrobial activity of the essential oils of Artemisia herba alba. The essential oils obtained by hydrodistillation from branches were analyzed by GC and GC/MS. The most abundant compounds identified vary according to their origins: á-thujone (17,52 to 48,91%), chrysanthenone (26,64 to 48,11%), 1-4 cineole (0,32 to 9,7%), artemisia alcohol (1,83 to 8,65%), camphor (0,04 to 6,68%) and á-pinene (2,21 to 6,42%).. The influence of the provenance on the antimicrobial activity of these essential oils was also highlighted.
Essential Oil Composition of Artemisia Vulgaris Grown in Egypt
International Journal of Pharmacy and Pharmaceutical Sciences, 2016
Objective: The objective of this research was to evaluate the significance of the plant's origin and to assess the essential oil composition of Artemisia vulgaris grown in Egypt simultaneously evaluating the effect of environmental conditions on essential oil composition.
Analysis of the Essential Oil of Artemisia Absinthium in Two Harvest Times
REVISTA FOCO
Artemisia absinthium, also known as wormwood, is used in traditional medicine as anti-parasitic and analgesic. The aim of the present study was to evaluate the effect of four doses of mineral fertilization in two harvest times on the content and composition of the essential oils produced by A. absinthium. The tested doses of mineral fertilization were the following: without fertilization (D0); 50% of the recommended dose (D1); recommended dose (D2); and 150% of the recommended dose (D3). The plants were cultivated in 10 L pots kept in greenhouse. The samples for extracting the essential oils were collected at 60 and 125 days after transplantation (DAT). The essential oils were obtained by hydrodistillation using a Clevenger-type apparatus. Identification and relative percentage of the compounds in the essential oils were performed by GC-MS. The essential oils content varied from 0.04% to 0.41% w/w. The highest essential oil content obtained was 0.41% using the dose D3 at 120 DAT. My...
Essential Oil Composition from Artemisia campestris Grown in Algeria
Aerial parts of Artemisia campestris (Asteraceae), collected in the flowering phase, from Boussaada region (Algeria) were analysed for their volatile components. The volatile fraction was isolated by hydrodistillation in a Clevenger-type apparatus for 3 h and analysed by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). The essential oil was obtained in a yield of 0.66% (v/dry weight). Fifty-eight components were identified representing 98% of the total oil. The main components were α-terpenyl acetate and α-pinene (19% and 18% respectively) followed by camphor (9%), camphene (8%), limonene and borneol (5% both).
Chemistry & Biodiversity, 2010
The intraspecific chemical variability of essential oils (50 samples) isolated from the aerial parts of Artemisia herba-alba Asso growing wild in the arid zone of Southeastern Tunisia was investigated. Analysis by GC (RI) and GC/MS allowed the identification of 54 essential oil components. The main compounds were b-thujone and a-thujone, followed by 1,8-cineole, camphor, chrysanthenone, transsabinyl acetate, trans-pinocarveol, and borneol. Chemometric analysis (k-means clustering and PCA) led to the partitioning into three groups. The composition of two thirds of the samples was dominated by athujone or b-thujone. Therefore, it could be expected that wild plants of A. herba-alba randomly harvested in the area of Kirchaou and transplanted by local farmers for the cultivation in arid zones of Southern Tunisia produce an essential oil belonging to the a-thujone/b-thujone chemotype and containing also 1,8-cineole, camphor, and trans-sabinyl acetate at appreciable amounts. Introduction.-Artemisia herba-alba Asso (Asteraceae) is a silvery-green, perennial dwarf shrub growing in semiarid and arid climates. This species is known as desert wormwood in English, armoise blanche in French, and chih in Arab, and it is characteristic of the steppes and deserts of the Middle East (Egypt and Israel), North Africa (Tunisia, Algeria, and Morocco), and Southern Europe (Spain and Italy), extending into the Northwestern Himalaya [1]. In Tunisia, A. herba-alba is found from the mountains around Jebel Oust (Fahs) until the South of the country [2] [3]. The aerial parts of A. herba-alba are widely used in the traditional medicine to treat diabetes, bronchitis, diarrhea, and neuralgias [4-6]. The essential oil of this species is known for its disinfectant, anthelmintic, and antispasmodic therapeutic virtues [7]. Also the antibacterial and the antispasmodic activities of A. herba-alba essential oil from various chemotypes have been examined [8]. Moreover, the oil exhibited antileishmanial [9], spasmolytic [10], and antimutagenic activity against the carcinogen benzopyrene [11]. According to Lawrence [12] and Salido et al. [13], the aerial parts of A. herba-alba produce an essential oil characterized by a tremendous chemical variability. Various chemotypes have been reported in almost all the countries where A. herba-alba grows wild. Briefly, two types of oils could be distinguished: i) oils with a composition dominated by one major compound, i.e., camphor, a-thujone, b-thujone, chrysanthenone, chrysanthenyl acetate, or davanone, and ii) oils characterized by the occurrence at appreciable contents of two or more of these compounds.