Phenolic Composition and Skin-related Properties of the Aerial Parts Extract of Different Hemerocallis Cultivars (original) (raw)
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The essential oil composition of selected Hemerocallis cultivars and their biological activity
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The horticultural cultivars of Hemerocallis (daylily) have been used to treat diseases such as insomnia, inflammation and depression, and also as a vegetable in eastern Asia. Taking into consideration the fact, that the volatile compounds in Hemerocallis cultivars have not been investigated to date, we decided to study the composition of the essential oils (EOs) from the aerial parts of ten varieties collecting in Poland. EOs, obtained by hydrodistillation, were analyzed by GC/MS method that resulted in identification of 23-36 volatile compounds comprising 89.5%–96.3% of the total amount. The essential oils differed in their composition and they can be classified into three groups. The antibacterial and antioxidant activities of EOs were also evaluated. Gram-negative strains were most strongly inhibited by all tested oils. Two model systems have been used for the antioxidant efficacy, 2,2-diphenyl-1-picryl-hydrazyl (DPPH•) andβ-carotene bleaching assays. The essential oils with the ...
International Journal of Pharmacy and Pharmaceutical Sciences, 2015
Objective: The investigation was carried out to prepare 95 and 50% ethanol extract of Hemionitis arifolia leaves, and for the identification and quantification of the phytoconstituents present in it. Methods: Soxhlet extraction method was followed to prepare the extracts, followed by standard phytochemical methods to identify the phytoconstituents present in it. Paper chromatography, HPLC, LC MS/MS and LCMS studies were then done for the confirmation, quantification of phytoconstituents and detection of mass values of other unidentified phytoconstituents present in the extract. Results: Extractive values were found to be 14.6 and 15.6% w/w respectively. In the phytochemical studies, presence of carbohydrates, glycosides, saponins, flavonoids, phenolics, tannins, phytosterols and fixed oils was identified. On doing paper chromatography with Whatman No.1 filter paper using 15% acetic acid solvent system, six different spots with yellow-orange colour were detected under UV visualization. Out of the six spots, four were identified as quercetin, kaempferol, apigenin and rutin by co-chromatography. In the RPHPLC analysis, rutin, quercetin and kaempferol were identified and quantified. In the LC MS/MS analysis, rutin, apigenin and kaempferol were identified and quantified. In the LCMS analysis, ten different m/z values were found, among which m/z 609 was identified as rutin. Conclusion: The presence of various flavonoids and related glycosides is indicative of potential for medicinal use of this plant.
Phytoconstituents Present in the Hemionitis Arifolia Leaves
2015
Objective: The investigation was carried out to prepare 95 and 50% ethanol extract of Hemionitis arifolia leaves, and for the identification and quantification of the phytoconstituents present in it. Methods: Soxhlet extraction method was followed to prepare the extracts, followed by standard phytochemical methods to identify the phytoconstituents present in it. Paper chromatography, HPLC, LC MS/MS and LCMS studies were then done for the confirmation, quantification of phytoconstituents and detection of mass values of other unidentified phytoconstituents present in the extract. Results: Extractive values were found to be 14.6 and 15.6% w/w respectively. In the phytochemical studies, presence of carbohydrates, glycosides, saponins, flavonoids, phenolics, tannins, phytosterols and fixed oils was identified. On doing paper chromatography with Whatman No.1 filter paper using 15% acetic acid solvent system, six different spots with yellow-orange colour were detected under UV visualization. Out of the six spots, four were identified as quercetin, kaempferol, apigenin and rutin by co-chromatography. In the RPHPLC analysis, rutin, quercetin and kaempferol were identified and quantified. In the LC MS/MS analysis, rutin, apigenin and kaempferol were identified and quantified. In the LCMS analysis, ten different m/z values were found, among which m/z 609 was identified as rutin. Conclusion: The presence of various flavonoids and related glycosides is indicative of potential for medicinal use of this plant.
Flavonoid compounds and their antioxidant activity in extract of some tropical plants
J. Med. Plants …, 2012
The study was counducted to investigate flavonoids content and antioxidant activities in some tropical plants namely cabbage (Brassica oleracea), green chilli (Capsicum annum), red chilli (Capsicum annum), carrot (Daucus carota), red spinach (Amaranthus gangeticus), white radish (Raphanus sativus), lemon grass (Cymbopogon citratus) and turmeric (Curcuma longa). Total flavonoid (TF) content was observed to be highest in red chili (0.939 mg/g DW) and low content of TF was observed in red spinach (0.066 mg/g DW). High performance liquid chromatography (HPLC) was employed to identify and quantify the five important flavonoid components (quercetin, catechin, kaempferol, apigenin and rutin). High content of quercetin (0.639 mg/g DW), catechin (0.14 mg/g DW), apigenin (0.047 mg/g DW) and rutin (0.069 mg/g DW) was detected from red chilli and high content of kaempferol (0.13 mg/g DW) was detected from carrot. Red chilli with high flavonoid compounds showed highest antioxidant activity (78.66%) determined by the 1, 1-diphenyl-2-picryl-hydrazyl (DPPH) assay. Results suggested a good potential of flavonoid compounds and antioxidant activity in red chilli and this plant could be useful for both food flavourings and in traditional medicine. Key words: Total flavonoid, high performance liquid chromatography (HPLC), red chilli, antioxidant activity.
Phenolic content and antioxidant activity of medicinal plants
Research, Society and Development
Medicinal plants have played an essential role in the development of human culture. Today, the contribution of plants in industries such as fine chemicals, cosmetics, pharmaceuticals, drugs, and industrial raw materials is remarkable. Recent studies have shown that plants have constituents with functional properties that effectively treat and prevent chronic degenerative diseases. Based on the importance of medicinal plants, this study aimed to quantify the total phenolic content and antioxidant activity of 10 medicinal plants. The phenolic content was quantified using the Swain and Hillis method with a Folin-Ciocalteau reagent, while the antioxidant activity was determined using a 2,2-diphenyl-1-picrylhydrazyl (DPPH) reagent. The samples were dried in a circulating air incubator at 60 ºC for 48 h before phenolic extraction. The compounds were extracted with 50% v/v ethanol, 50% v/v acetone, and distilled water. The results of this study showed that 80% of the plants analyzed had si...
Food Chemistry, 2008
Barks extracts of four different trees (Azadirachta indica, Terminalia arjuna, Acacia nilotica, and Eugenia jambolana Lam.) in three different solvents 80% methanol, 80% ethanol, and 80% acetone (solvent:water, 80:20 v/v) were evaluated for their antioxidant activity, total phenolic (TP), and total flavonoids (TF) contents. Antioxidant activity (AA) was determined by measuring reducing power, inhibition of peroxidation using linoleic acid system and 2,2 0 -diphenyl-1-picrylhydrazyl radical (DPPH) scavenging activity. Significant (P < 0.05) differences were observed in the TP, TF, inhibition of linoleic acid oxidation and DPPH Á scavenging activity of different bark extracts. Nevertheless, minute variation was observed in reducing power. All the bark extracts exhibited wide range of total phenolic, 7.8-16.5 gallic acid equivalents and total flavonoid contents, 1.59-4.93 catechin equivalents. Reducing power at 10 mg/mL extract concentration ranged from 1.34 to 1.87. Different bark extracts inhibited oxidation of linoleic acid by 44-90% while DPPH radical scavenging activity ranged from 49% to 87%. Extraction efficacy of components with antioxidative properties was lowering in the following order: ethanol > methanol > acetone. Good correlation was observed between TP and DPPH scavenging activity among the extracts. A. nilotica bark had the highest amounts of TP, ranging from 9.2 to 16.5 g/100 g, while the highest AA as measurement by inhibition of linoleic acid oxidation is offered by bark from E. jambolana Lam. The same tree showed the highest DPPH scavenging activity and reducing power. The correlation among the results of different antioxidant assays although revealed a strong relationship between some of the assays, however, a number of different methods may be necessary to adequately assess the in vitro antioxidant activity of a specific plant material.
Phytochemical Analysis, 2010
Introduction -Polyphenolic phytochemicals in traditionally used medicinal plants act as powerful antioxidants, which aroused an increasing interest in their application in functional food development. Objective -The effect of extraction time (5 and 15 min) and hydrolysis on the qualitative and quantitative content of phenolic compounds and antioxidant capacity of six traditionally used medicinal plants (Melissa officinalis L., Thymus serpyllum L., Lavandula officinalis Miller, Rubus fruticosus L., Urtica dioica L., and Olea europea L.) were investigated.
Comparative Study of Phenolic Content of Some Plant Extracts
Banat's Journal of Biotechnology, 2012
The aim of the present study was to draw a comparative analysis of the content in flavones and polyphenols of the hydro alcoholic extracts obtained from two medicinal plants, namely artichoke (leaves) and respectively, licorice (root) according to the origin of the vegetal material and the method used in the preparation of the extracts. The plants used in the experiments are two indigenous medicinal plants belonging to wild and cultivated flora in Romania. Artichocke (Cynara scolymus) and licorice (Glycyrrhiza glabra) were purchased from three local companies trading medicinal plants: Plafar, Vitaplant and Franco Impex. It was evaluated the composition of the extracts in terms of the content of polyphenolic compounds expressed in caffeic acid and flavone compounds content expressed in rutin. Plant extracts were obtained by two different methods of preparation: ultrasonation and maceration with intermittent shaking.
Journal of the University of …, 2011
Herbs are an ancient source of flavouring, aromatic compounds and medicines, not only for culinary application. The increasing interest in the powerful biological activity of plant phenolics and flavonoids outlined the necessity of determining their content in medicinal herbs. In this present study, a comparative evaluation of the polyphenol composition, antioxidant capacity and biological contaminants (microbes and other organisms) as major common contaminants in medicinal herbs from the Lamiaceal family to which belong: lemon balm (Melissa officinalis), sage (Salvia officinalis) and mint (Mentha piperita) were carried out. The total phenolic and total flavonoid contents in medicinal herbs ware evaluated using the Folin-Ciocalteu method, were determined with an aluminum chloride colorimetric assay. The 2,2-diphenyl-1-picryl hydrazyl (DPPH) radical scavenging effect of the herbs was measured also spectrophotometrically, like the total phenolic and total flavonoid contents. Microbiology was investigated using the current ISO methods. The present paper shows by the results of total phenolic and total flavonoid contents, antioxidant activity and biological contaminants in medicinal herbs that they must be relatively safe for the patient (consumer).
Relationship Between Phenolic Content And Antioxidant Capacity In Seven Traditional Medicinal Plants
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Antioxidant potential of seven medicinal plants through total phenolic content using Folin - Ciocalteu's method has been studied in which five phenolic compounds were quantified by HPLC and their antioxidant activity was evaluated using DPPH radical scavenging and total antioxidant activity methods. Moreover, the correlation between their total phenolic content and chemical compositions with total antioxidant capacity was also analyzed. The data resulted from DPPH radical scavenging activities indicated that they displayed the good activities with low IC50 values. Importantly, L. rubra had the highest activities, approximately 32 times less than that of curcumin. Basex on the total antioxidant activity and HPLC analysis, the antioxidant capacity of H. parasitica wass found the highest among seven medicinal plants. The amount of the five phenolic compounds is closely correlated with either total phenolic compounds or total antioxidant capacity. Obviously, phenolic compounds were ...