The effects of whortleberry on ischemia reperfusion-induced myocardial injury in rats (original) (raw)
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Resveratrol against lung injury in an ischemia/reperfusion model of abdominal aortic rupture
Turkish Journal of Thoracic and Cardiovascular Surgery, 2021
Bu çalışmada sıçanlarda rüptüre abdominal aort anevrizması modelinde iskemi/reperfüzyon hasarının akciğerlerdeki etkileri incelendi ve resveratrolün olası koruyucu etkileri araştırıldı. Ça lış ma pla nı: Otuz iki erkek Sprague-Dawley sıçanı rastgele dört gruba ayrıldı: kontrol, iskemi/reperfüzyon, sham (iskemi/ reperfüzyon+solvent/dimetil sülfoksit) ve iskemi/reperfüzyon +resveratrol. İskemi/reperfüzyon uygulanan gruplarda abdominal aorta 60 dk. şokun ardından infrarenal ve iliyak bifurkasyon seviyelerinden vasküler klempler yerleştirildi. Toplamda 60 dk. iskemiyi takiben 120 dk. reperfüzyon uygulandı. İskemi/reperfüzyon + resveratrol grubuna, iskemiden 15 dk. önce ve reperfüzyondan hemen önce intraperitonal yoldan 10 mg/kg resveratrol uygulandı. Malondialdehit, glutatyon ve katalaz düzeyleri araştırıldı ve akciğer dokusunda histopatolojik değerlendirme yapıldı. Bul gu lar: Kontrol grubuna kıyasla iskemi/reperfüzyon ve iskemi/ reperfüzyon + dimetil sülfoksit gruplarında, malondialdehit düzeyleri arttı, katalaz düzeyleri azaldı ve glutatyon düzeylerinde anlamlı bir fark gözlenmedi. Resveratrol uygulanması ile malondialdehit düzeyleri azalırken, glutatyon düzeyleri arttı ve katalaz düzeyleri değişmedi. Ayrıca, iskemi/reperfüzyon ve iskemi/reperfüzyon + dimetil sülfoksit gruplarında gözlenen interstisyel alanlarda enflamasyon artışı, damarlarda hiyalin membran yapılarının birikimi, alveolar septal duvarında kalınlaşma ve cleaved caspase-3 pozitif apoptotik pnömositlerin sayısındaki ve akciğer histopatolojik tahribat skorundaki artış resveratrol uygulanması ile geriledi. So nuç: Bu bulgular, resveratrolün rüptüre abdominal aort anevrizması cerrahisinde iskemi/reperfüzyon nedeniyle gelişen akut akciğer hasarının önlenmesinde oksidatif tahribatı azaltarak koruyucu etkinliği olabileceğini göstermektedir.
Ankaferd® Kanama Durdurucunun Rat Abdominal Aort Kanama Modelinde Kanama Zamanına Etkisi
Kosuyolu Heart Journal, 2016
Introduction: The use of topical haemostatic agents has been popularised in recent years in many areas of surgery. We aimed to determine whether Ankaferd blood stopper ® (ABS) can shorten the bleeding time and decrease the amount of bleeding in a rat abdominal aortic bleeding model. Patients and Methods: A total of 40 rats were randomly divided into four groups (n= 10 in each group). Saline-and ABS-soaked gauzes were applied to rats that had not received any pre-treatment in Groups 1 and 2, while saline-and ABS-soaked gauzes were applied to rats that had received acetylsalicylic acid plus oral clopidrogel for 5 days in Groups 3 and 4. An incision of about 1 mm was made over the aorta just proximal to the iliac bifurcation. Mean bleeding time (MBT) and mean bleeding amount (MBA) were determined and compared. Results: There were no signifi cant differences in the age, weight and blood sample laboratory results of rats. Shorter MBT and less MBA were determined in ABS-soaked groups and the differences were statistically signifi cant. Conclusion: Ankaferd blood stopper ® has the potential to reduce the bleeding time and post-operative blood loss after a major aortic surgery.
2014
Özet– Kateter yoluyla aort kapağı yerleştirilmesi son yıllarda cerrahi açısından yüksek riskli, ciddi aort darlığı olan semptomatik hastalarda alternatif bir tedavi yöntemi haline gelmiştir. Ancak bu yeni tedavi yöntemi ile birlikte bazı ölümcül komplikasyonlar da görülmeye başlamıştır. Bunlardan biri işlem esnasında gelişen sol ana koroner arter ağzındaki tıkanıklıktır. Bu yazıda, kateter yoluyla aort kapağı yerleştirilmesi sırasında sol ana koronerde tama yakın akut tıkanma ve hemodinamik bozukluk gelişen ve başarılı balon anjiyoplasti ve stent işlemi uygulanan bir olgu sunuldu. Summary– Transcatheter aortic valve implantation is becoming increasingly popular as an alternative treatment technique for symptomatic patients with severe aortic stenosis, who are at high risk for surgery. However, with this revolutionary treatment modality, some fatal complications have also emerged. One of these fatal complications is the occlusion of the left main coronary artery at the time of proced...
OSMANGAZİ JOURNAL OF MEDICINE, 2019
Abdominal aortic aneurysm (AAA) has a high prevalence and surgical treatment is still the best option for many reasons. Surgical treatment needs abdominal blood flow to be discontinued, which leads to secondary complications caused by ischemiareperfusion (I/R) in different organs. Since distant organ damages are seen after surgery, abdominal ischemia reperfusion results in post-treatment deaths especially due to heart based problems. In this study, we aimed to investigate the possible alterations in action potential parameters of papillary muscle after abdominal I/R injury. Adult Wistar-Albino rats were divided into two groups randomly: SHAM group (only laparotomy was performed) and I/R group (abdominal aorta was clamped for 1 hour and reperfused for 2 hours). After the operational period, left ventricle papillary muscles were isolated and action potential (AP) recording experiments were carried out in-vitro. Significant hyperpolarization was seen in resting membrane potential in I/R group. There was no alteration in the general shape of the action potential after I/R. Some delayed-after-depolarizations were recorded, that suggests an impaired persistent Na+ channel activity when interpreted with resting membrane potential findings.This study shows that I/R does not affect the AP parameters except for resting membrane potential. However, it is also likely that the sodium-calcium exchanger (NCX) causes delayed arrhythmias after depolarization due to dysfunction of unknown causes.
Effects of Potentilla Fulgens as Prophylactic Agent in Intestinal Ischemia Reperfusion Injury
International Journal of Morphology, 2015
El objetivo fue investigar los efectos protectores de Potentilla fulgens sobre la lesion por isquemia-reperfusion en ratas albinas Wistar (n= 30) con un peso de 180 g. En el grupo 1, los animales fueron sometidos a laparotomia simulada sin lesion por isquemia-reperfusion. En el Grupo 2, los animales fueron sometidos a laparotomia y oclusion de las arteria mesenterica superior durante 30 min seguido de 20 min de reperfusion sin pretratamiento. El grupo Potentilla fulgens recibio 400 mg/kg/dia de P. fulgens por via intraperitoneal 5 dias antes de la lesion por isquemia-reperfusion. Hubo diferencias significativas entre el grupo de grupo con isquemia-reperfusion y el tratado con Potentilla fulgens (p<0,0001). En el analisis estadistico del nivel de malondialdehido (MDA), los datos se obtuvieron despues de una medicion respectiva en todos los grupos. Los grupos Potentilla fulgens y con isquemia-reperfusion tuvieron una disminucion significativade MDA (p<0,0001). En el periodo desp...
Koroner Angiografi İşlemi Esnasında Tesadüfen Saptanan Ciddi Aort Koarktasyonu
2014
Konjenital kalp hastaliklari icerisinde % 5-8 oraninda sikliga sahip aort koarktasyonuna bikuspit aort kapagi siklikla eslik etmektedir. Aort koarktasyonu tipik olarak cocukluk ve erken eriskin doneminin hastaligidir ve onarim yapilmazsa yasam suresi azalir. Onarim yapilmayan hastalarda olum; siklikla kalp yetersizligi, koroner arter hastaligi, aort rupturu/ diseksiyonu, enfektif endokardit/ endarterit veya serebral hemorajiye baglidir. Bu yazida, 60 yasinda cikan aort anevrizmasi, aort koarktasyonu ve bikuspid aort kapagi olan kararsiz angina pektoris ile acil servise gelen hasta takdim edilmistir
Effects of ukrain in rats with intestinal ischemia and reperfusion
Journal of Surgical Research, 2014
The purpose of this study is to investigate the potential protective effect of the ukrain on ischemia-reperfusion (IR) injury in rat intestine, which has not previously been studied. Thirty-one male Sprague-Dawley rats were randomly assigned to four groups, each consisting of eight rats as follows: (1) a sham group (S) (laparotomy, but no IR injury); (2) ukrain group (U) (no IR, and ukrain was administered intraperitoneally 1 h before laparotomy); (3) intestinal ischemia-reperfusion (II/R) group (30-min occlusion of the superior mesenteric artery then 2-h reperfusion); and (4) ukrain + II/R group (U + II/R) (30-min occlusion of the superior mesenteric artery then 2-h reperfusion; ukrain was administered intraperitoneally 1 h before IR). Serum total oxidant status (TOS) and total antioxidant status (TAS) were measured using Erel method. Oxidative stress index was calculated using the TOS/TAS ratio. TAS levels increased and TOS serum levels were also significantly decreased in the ukrain + IR group compared with the IR group (P = 0.000 and P = 0.015). In this study, we demonstrated for the first time in literature that ukrain helps to prevent intestinal tissue breakdown against II/R injury and that this effect can be achieved by antioxidant activities.
Green tea polyphenol extract attenuates ischemia/reperfusion injury of the gut
Naunyn-Schmiedeberg's Archives of Pharmacology, 2005
Various studies have clearly demonstrated that green tea catechins possess potent antioxidative properties, and the preventive effects against various oxidative diseases have been reported. The aim of this study was to investigate the effect of green tea extract on the tissue injury caused by ischemia/reperfusion (I/R) of the gut. I/R injury of the intestine was caused by clamping both the superior mesenteric artery and the celiac trunk for 45 min followed by release of the clamp allowing reperfusion for 1 h or 4 h. This procedure results in splanchnic artery occlusion (SAO) shock. Rats subjected to SAO developed a significant fall in mean arterial blood pressure, and only 10% of the animals survived for the entire 4-h reperfusion period. Surviving animals were sacrificed for histological examination and biochemical studies. Rats subjected to SAO displayed a significant increase in tissue myeloperoxidase (MPO) activity and malondialdehyde (MDA) levels, significant increases in plasma tumor necrosis factor (TNF)-α levels and marked injury to the distal ileum. Increased immunoreactivity to nitrotyrosine was observed in the ileum of rats subjected to SAO. Staining of sections of the ileum obtained from SAO rats with anti-intercellular adhesion molecule (ICAM-1) antibody and with anti-Pselectin antibody resulted in diffuse staining. Administration of green tea extract (20 and 10 mg kg −1 i.v.) 15 min prior to the onset of gut reperfusion significantly reduced in a dose-dependent manner the fall in mean arterial blood pressure, the mortality rate, infiltration of the reperfused intestine with polymorphonuclear neutrophils (MPO activity), lipid peroxidation (MDA levels), production of TNF-α, and histological evidence of gut injury. Administration of green tea extract also markedly reduced nitrotyrosine formation and the up-regulation of ICAM-1 and P-selectin during reperfusion. In order to clarify that green tea extract might be useful in the therapy of I/R injury, we also investigated the effect of green tea extract (20 mg kg −1 i.v.) when administered 5 min after the onset of gut reperfusion. Similar to the pretreatment approach, the post-treatment also significantly reduced the gut injury induced by I/R. These results demonstrate that green tea extract significantly reduces I/R injury of the intestine.
The objective of the current work aimed to investigate whether carvedilol is an effective antishock agent in the well established in vivo ischemia/reperfusion model of splanchnic artery occlusion shock. Also, to investigate the mechanism of this protection, such as antioxidant properties, preservation of endothelial function, and inhibition of neutrophil accumulation and adhesion. For this study, 36 rats weighing 120-200gm were selected. They were classified into 4 groups; control, splanchnic ischemia-reperfusion (SI/R), SI/R rats injected by 0.5mg/kg carvedilol and SI/R rats injected by 1.0 mg/kg carvedilol. Each group was formed of 9 rats. The results obtained showed that; hematocrite, tissue malondialdehyde (MDA), and tissue myeloperoxidase (MPO) were significantly increased (p<0.001) while plasma MDA and anti MPO were non-significantly increased in ischemic-reperfusion group compared with the control group. Comparative study of injected groups versus noninjected SI/R group, t...