In vitro antimicrobial appraisal of the potentials of Morinda lucida against some selected bacteria (original) (raw)
Related papers
Possible antimicrobial activity of Morinda lucida stem bark, leaf and root extracts
African Journal of Biotechnology, 2014
Inhibitory activities of both aqueous and methanolic extracts of the root, stem bark, and leaf of Morinda lucida on Escherichia coli, Salmonella typhi, Salmonella paratyphi, and Salmonella typhorium was investigated in vitro. In vitro experiment was carried out using the agar well diffusion and disc diffusion methods with Gram-negative enterobacteria. M. lucida extracts were more active against all the tested bacteria than the standard antibiotics, chloramphenicol and ciprofloxacin even at the same concentrations of 5, 10 and 20 mg/ml. The results of this study show that the extracts of M. lucida has the potentials of inhibiting the growth of E. coli and Salmonella species, thereby suggesting its potency in the treatment of infections in which E. coli and Salmonella species are implicated.
Antibacterial and toxicity studies of aqueous extracts of Morinda lucida
Development Journal of Science and Technology Research (DJOSTER), 2013
The aqueous extract of the leaves of Morinda lucida which is used Igala traditional folk medicine in Nigeria for several febrile ailments was investigated for its antibacterial activity against Escherichia coli, Staphylococcus aureus, and Salmonella Typhi, and for its acute toxicity in albino rats. The antibacterial activity was determined by the zone of inhibition using agar well diffusion method and determining the minimum inhibitory concentration. The toxicity screening was done by randomising 28 healthy albino rats into seven groups of 4 animals each and treated them p.o. with a various doses of the crude extract in 5 ml of normal saline, the control group received equal aliquot of the normal saline. Investigation showed that the water extract of M. lucida was very active against all the test organisms with antibacterial activities equal to those of the most active standard antibiotic against each of the test organisms, gentamicin for S. Typhi , chloramphenicol for S. aureus and gentamicin for E. coli. The LD 50 of M. lucida is >5000mg/kg b. wt. using albino rats. There were no apparent pharmacotoxic signs observed in animals that received p.o. doses of M. lucida. There were no macroscopic changes in the internal organs of any of the treated rats. The investigation also showed that the PCV and blood chemistry parameters of animals treated with the extract of M. lucida were within the reference range. Aqueous extract of M. lucida thus has potential for the development of a safe broad spectrum antibiotic.
The purpose of this research work is to determine the antimicrobial activities of crude ethyl acetate and ethanolic extracts of Morinda lucida leaf, stem, and bark growing in Adekunle Ajasin University Botanical Garden against selected clinical isolates. The plant Morindal lucida falls under the family Rubiaceae known to have wide usage in traditional medicine. Morinda lucida is a tropical West African tree of medium-size about 18–25 m tall, the bark is grey to brown in color, flowers are white in color, the fruit is a drupe, seed is ellipsoid, yellowish and soft. The antibacterial potency of ethyl acetate and ethanolic extracts of Murinda lucida leaf, stem, and bark were tested against selected clinical isolates, the clinical isolates are Escherichia coli (ATCC 0157), Escherichia coli (ATCC 25922), Salmonella typhi, Candida albicans (ATCC 90029), Klebsiella pneumonia (ATCC 35657), Mycobacterium fortuitum (ATCC 6841). Mycobacterium smegmatis (ATCC 19420), Mycobacterium abscessus (ATCC 19977), Mycobacterium phlei (ATCC 19240), Staphylococcus aureus (ATCC 29213). Agar well diffusion method was used to measure the antibacterial activities of Morinda lucida against the clinical isolates. We observed that the, Morinda lucida has antimicrobial activity against the clinical isolates. It was also observed that, the ethanol extracts of leaf and bark has better antimicrobial activity compared to the ethyl acetate stem extract. But all the extracts exhibit various degrees of antimicrobial activities. Citation: Osuntokun OT (2015). Evaluation of antimicrobial activities of crude ethyl acetate and ethanol extracts of Morinda lucida leaf, stem, and bark growing in Adekunle Ajasin University Botanical Garden against selected clinical isolates.
Phytochemical screening and antimicrobial study on the leaves of Morinda lucida (Rubiaceae)
An ethno-medicinal study was conducted through the means of an informal interview with an herbalist at Akim-Tafo, in the Eastern region of Ghana. Preliminary phytochemical tests carried out on the leaves of Morindalucida indicated the presence of saponins, anthraquinones, cardenolides, alkaloids, sterols and tannins. ThinLayer Chromatography and Column Chromatography method were used in the laboratory for the analysis of acrude extract from Morinda lucida. A total of nine components were isolated from Morinda lucida using asolvent system containing hexane and ethyl acetate (2:1) as the mobile phase and a stationary phase of silica gel.The crude leaf extract of Morinda lucida was investigated for inhibitory activity on Salmonella typhi,Pseudomonas aeruginosa and Staphylococcus aureus in vitro using the agar well diffusion method. Morindalucida showed inhibitory activity against Pseudomonas aeruginosa and Staphylococcus aureus with no activityagainst Salmonella typhi at a concentratio...
HAL (Le Centre pour la Communication Scientifique Directe), 2018
The essential oils (EOs) obtained by hydro-distillation of fruits of Morinda lucida (Rubiaceae) growing in Côte d'Ivoire were investigated. The oil was analyzed and characterized by GC and GC-MS. Analyses of the EOs led to the identification and quantification of 45 constituents. Characterization of the EOs revealed the predominance of (E)-phytol (14.80 %) and n-octanol (6.19 %) followed by β-caryophyllene (5.54 %) and 6,10,14-trimethyl pentandecan-2-one (5.48 %). The antibacterial activity of EOs from M. lucida was tested against three bacteria Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa using a disc-diffusion method and the minimal inhibitory concentration (MIC) values determined. The inhibition zones and MIC values were in the range of 11.6 to 24.3 mm and 32 to 256 μg/mL, respectively. The EOs showed good antibacterial activity against S. aureus and P. aeruginosa. The volatile oils extracted from M. lucida exhibited a higher activity than the antibiotics tested on P. aeruginosa.
International Journal of Scientific World, 2017
The leaves of two medicinal plants namely; Morinda lucida and Nauclea latifolia were investigated for their antibacterial, mineral and phytochemical properties. Results of the study showed that the aqueous and ethanolic extract of the two leaves had significant antibacterial activity against Escherichia coli, Staphylococcus aureus, Pseudomonas aeruginosa, Listeria monocytogenes and Proteus mirabilis. The biologically active constituents present in the extracts were cardiac glycosides, tannins, saponin, coumarins, reducing sugar, terpenoids, alkaloids, steroids and flavonoids. The percentage yield extracts of the respective plants were Morinda lucida leaves 12.9% for ethanol and 9.0% for aqueous extracts while Nauclea latifolia had 12.1% for ethanol and 8.4% for aqueous extracts. Results of the antibacterial activity analysis revealed that the ethanolic extracts of the two medicinal plants at different concentrations were more active against the test organisms named above than the aq...
The plant Morinda lucida falls under the family Rubiaceae known to have wide usage in traditional medicine. Morinda lucida is a tropical West African tree of medium-size about 18–25 m tall, the bark is grey to brown in colour, flowers are white in colour, the fruit is a drupe, seed is ellipsoid, yellowish and soft. The purpose of this research work is to determine the antimicrobial properties of Morinda lucida against Mycobacterium species, a very virulent and infectious organism, isolated from the Chest Hospital, Obafemi Awolowo University, Ile-Ife, Osun State, Nigeria. The method used to determine the antimicrobial potency of the plant extracts is the Agar well diffusion method. The antibacterial potency of Ethyl acetate and ethanol extracts of Morinda lucida leaf, stem, and bark were tested against mycobacterium species including Mycobacterium fortuitum (ATCC 6841), Mycobacterium smegmatis (ATCC 19420, Mycobacterium abscessus (ATCC 19977), and Mycobacterium phlei (ATCC 19240). All extracts exhibited various degree of antibacterial potency against the test organisms with the ethyl acetate extracts of the leaf and bark being the most active and ethanol extracts were the least active. The zone of inhibition of ethyl acetate leaf, bark and stem extracts range between 3.0 mm to 18.0 mm and the zone of inhibition of ethanol leaf, bark and stem extracts ranges between 1.0 mm to 10.0 mm respectively.
This study aimed at evaluating the health benefits of popular Moringa oleifera leaf. The aqueous and methanolic extracts of the leaf at two different concentrations (1:1 and 1:2) was used to determine the phytochemical screening and its antibacterial activity. Escherichia coli, Streptococcus pneumonia and Staphlococcus aureus were used in this study, applying agar diffusion methods. The phytochemical screening indicated presence of secondary metabolites such as alkaloid, flavonoids, anthraquinones, tannins and phenol in both extracts making it to have antibacterial potentials. Both extract showed remarkable activity against the growth of the selected bacteria; nevertheless, the methanol extract had more antibacterial activity than the water extract, more so the extracts were discovered to be more active at higher concentration. The water extract was not active at low concentration, that is 1:1 but had diameter zone of inhibition of 10 mm each for 1:2 concentration. The minimum inhibition concentration (MIC) that inhibits these bacterial ranged between 1:4 and 1:16 and the minimum bactericidal concentration (MBC) that kills the growth of the bacterial isolates completely was 1:16. The result of this study showed that M. oleifera could be a valuable antibacterial drug in the treatment of infections caused by the test organisms.
Antimicrobial Activities and Phytochemical Investigation of Moringa Oleifera Lam. Leaf Extracts
https://www.ijrrjournal.com/IJRR\_Vol.4\_Issue.4\_April2017/Abstract\_IJRR002.html, 2017
Phytochemicals investigation and Antimicrobial activities of water and methanol extracts of Moringa Oleifera leaf were investigated in an attempt to evaluate its antimicrobial potentials. The inhibition was determined using Ditch method against four selected bacterial species and three fungal species. The phytochemical screening indicated the presence of alkaloids, flavonoids, glycoside, saponin, steroids, tannins and volatile oil. Both water and methanol extracts showed highest antifungal activity against S. cerevisiae with zones of inhibition of 9, 12 and 14 mm at the concentration of 60, 90 and 120 mg/ml respectively in water extract; and inhibition zones of 11, 15, 16 and 18 mm at the concentration of 30, 60, 90 and 120 mg/ml respectively in methanol leaves extract of M. oleifera. A flavus on the other hand was the resistant fungal species to the M. Oleifera extracts showing no visible zone of inhibition in water extract; and exhibiting inhibition in methanol with 7 and 9.5 mm zones of inhibition at 90 and 120 mg/ml respectively. The antifungal standard drug ketoconazole exhibited highest activity against C. albicans with 19 mm zone of inhibition, followed by S. cerevisiae 15 mm zone of inhibition. In methanol extracts against Pseudomonas aeruginosa shows the highest inhibition of 15.00 mm at the highest concentration (120mg/ml), while Escherichia coli and Micrococcus species had 14.00 mm and Staphylococcus aureus with least inhibition of 8.33mm at the lowest concentration (30mg/ml). The zones of inhibition of tetracycline on test bacteria showed that P. aeruginosa was the most susceptible with the diameter of 21 mm, followed by S. aureus with 20 mm. The results obtained provide a support for the utilization of this plant in traditional medicine and recommend its further investigation of it phytochemicals.
This study aimed at evaluating the health benefits of popular Moringa oleifera leaf. The aqueous and methanolic extracts of the leaf at two different concentrations (1:1 and 1:2) was used to determine the phytochemical screening and its antibacterial activity. Escherichia coli, Streptococcus pneumonia and Staphlococcus aureus were used in this study, applying agar diffusion methods. The phytochemical screening indicated presence of secondary metabolites such as alkaloid, flavonoids, anthraquinones, tannins and phenol in both extracts making it to have antibacterial potentials. Both extract showed remarkable activity against the growth of the selected bacteria; nevertheless, the methanol extract had more antibacterial activity than the water extract, more so the extracts were discovered to be more active at higher concentration. The water extract was not active at low concentration, that is 1:1 but had diameter zone of inhibition of 10 mm each for 1:2 concentration. The minimum inhibition concentration (MIC) that inhibits these bacterial ranged between 1:4 and 1:16 and the minimum bactericidal concentration (MBC) that kills the growth of the bacterial isolates completely was 1:16. The result of this study showed that M. oleifera could be a valuable antibacterial drug in the treatment of infections caused by the test organisms.